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Comparative untargeted proteomic analysis of ADME proteins and tumor antigens for tumor cell lines

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Acta Pharmaceutica Sinica B 2018年第2期8卷 252-260页

作者： Xiaomei Gu Qing Xiao Qian Ruan Yuezhong Shu Ashok Dongre ramaswamy Iyer W.Griffith Humphreys Yurong Lai Pharmaceutical Candidate Optimization Bristol-Myers Squibb Company Genomics Bristol-Myers Squibb Company

In the present study, total membrane proteins from tumor cell lines including HepG2, Hep3 B2,H226, Ovcar3 and N87 were extracted and digested with γLys C and trypsin. The resulting peptide lysate were pre-fractionated and subjected to untargeted quantitative proteomics analysis using a high resolution mass spectrometer. The mass spectra were processed by the Max Quant and the protein abundances were estimated using total peak area(TPA) method. A total of 6037 proteins were identified, and the analysis resulted in the identification of 2647 membrane proteins. Of those, tumor antigens and absorption,metabolism, disposition and elimination(ADME) proteins including UDP-glucuronosyltransferase,cytochrome P450, solute carriers and ATP-binding cassette transporters were detected and disclosed significant variations among the cell lines. The principal component analysis was performed for the cluster of cell lines. The results demonstrated that H226 is closely related with N87, while Hep3 B2 aligned with HepG2. The protein cluster of Ovcar3 was apart from that of other cell lines investigated. By providing for the first time quantitative untargeted proteomics analysis, the results delineated the expression profiles of membrane proteins. These findings provided a useful resource for selecting targets of choice for anticancer therapy through advancing data obtained from preclinical tumor cell line models to clinical outcomes.

关键词： Cancer cell lines Untargeted quantitative proteomics Tumor-associated membrane proteins Cytochrome P450 ABC transporters SLC transporters

Nature-inspired topographies on hydroxyapatite surfaces regulate stem cells behaviour

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Bioactive Materials 2021年第4期6卷 1107-1117页

作者： Yogambha ramaswamy Iman Roohani Young Jung No Genevieve Madafiglio Frank Chang Furong Zhao Zufu Lu Hala Zreiqat Biomaterials and Tissue Engineering Research Unit School of Biomedical EngineeringUniversity of SydneySydneyNSW2006Australia Australian Research Centre for Innovative BioEngineering University of SydneySydneyNSW2006Australia School of Chemistry Australian Centre for NanomedicineUniversity of New South WalesSydney NSWAustralia

Surface topography is one of the key factors in regulating interactions between materials and *** topographies presented to cells in vivo are non-symmetrical and in complex shapes,current fabrication techniques are limited to replicate these complex *** this study,we developed a microcasting technique and successfully produced imprinted hydroxyapatite(HAp)surfaces with nature-inspired(honeycomb,pillars,and isolated islands)*** in vitro biological performance of the developed non-symmetrical topographies was evaluated using adipose-derived stem cells(ADSCs).We demonstrated that ADSCs cultured on all HAp surfaces,except honeycomb patterns,presented well-defined stress fibers and expressed focal adhesion protein(paxillin)*** islands topographies significantly promoted osteogenic differentiation of ADSCs with increased alkaline phosphatase activity and upregulation of key osteogenic markers,compared to the other topographies and the control unmodified(flat)HAp *** contrast,honeycomb topographies hampered the ability of the ADSCs to proliferate and differentiate to the osteogenic *** work presents a facile technique to imprint nature-derived topographies on the surface of bioceramics which opens up opportunities for the development of bioresponsive interfaces in tissue engineering and regenerative medicine.

关键词： Stem cell Biomaterials Bioceramics Scaffolds Micropattern Topography

Hepatoprotective efficiency of methanol extract of red algae against chromium-induced oxidative damage in Wistar rats

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Journal of Coastal Life Medicine 2016年第7期4卷 541-546页

作者： Murugesan Subbiah bhuvaneswari Sundaresan Kalandar,Ameer Sivamurugan Vajiravelu Division of Algal Biotechnology and Bionano Technology Post Graduate and Research Department of BotanyPachaiyappa’s CollegeChennai600030.India Faculty of Medical Sciences Haramaya UniversityHararEthiopia Post Graduate and Research Department of Chemistry Pachaiyappa’s CollegeChennai600030India

Objective:To investigate the hepatoprotective activity of red algae Portieria hornemannii(Lyngbye)Silva(***)and Spyridia fusiformis Boergesen(***)by using the chromium treated rat liver as the animal ***:The extract of red algae at a dosage of 0.200 g/kg of whole body weight was orally administrated to Cr(VI)intoxicated rats for 28 consecutive *** effect of drug in rats was evaluated by comparing the degree of the production of enzymes responsible for antioxidant activity such lipid peroxidase,superoxide dismutase,catalase and reduced glutathione with Cr(VI)analogs in the absence of any secondary *** overall damage of liver was detected by measuring serum enzymes such as aspartate amino transferase and alanine aminotransferase activities which released into the blood from the damaged ***:It was observed that these enzyme levels were noticed in the animals treated with methanol extracts of red algae(200 mg/kg)through preventing the leakage of the above enzymes into the *** hepatoprotection obtained using LIV 52(standard reference drug)appeared relatively *** antihepatotoxic potential of red algae *** and *** might be due to their antioxidative and membrane stabilizing ***:Our results indicated that the extract of *** and *** obtained from methanol could be a promising hepatoprotective agent against chromium(VI)-induced liver damage.

关键词： Oxidative liver injury Red algae Chromium(VI) Hepatoprotection Lipid peroxidantion Antioxidant enzymes

A Novel Part-Winding Starting Method for 4-Pole Squirrel Cage Induction Motor

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Engineering（科研） 2010年第6期2卷 445-452页

作者： Vadugapalayam Ponnuvel Sakthivel Ramachandran bhuvaneswari Srikrishna Subramanian 不详

the paper has been removed due to the authors' personal reason.

关键词： Induction Motor Starting Part-Winding Starting Starting Torque Starting Current

Economic Design of Three-Phase Induction Motor by Particle Swarm Optimization

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Journal of Electromagnetic Analysis and Applications 2010年第5期2卷 301-310页

作者： Vadugapalayam P. Sakthivel Ramachandran bhuvaneswari Srikrishna Subramanian 不详

A Particle Swarm Optimization (PSO) based design of three-phase induction motors are proposed. The induction motor design is treated as a non-linear and multivariable constrained optimization problem. The annual material cost and the total annual cost of the motor are chosen as two different objective functions. The PSO is used to find a set of optimal design variables of the motor which are then used to predict performance indices and the objective functions. The proposed method is demonstrated for two sample motors, and it is compared with the genetic algorithm (GA) and the conventional design methods. The results show that the PSO-based method effectively solved the induction motor design problems and outperforms the other methods in both the solution quality and computation efficiency.

关键词： Design Optimization Induction Motor Particle Swarm Optimization

Barcelona Smart City: The Heaven on Earth(Internet of Things: Technological God)

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ZTE Communications 2015年第4期13卷 3-9页

作者： Somayya Madakam ramaswamy Ramachandran National Institute of Industrial Engineering

Cities are the most preferable dwelling places, having with better employment opportunities, educational hubs, medical services, recreational facilities, theme parks, and shopping malls etc. Cities are the driving forces for any national economy too. Unfortunately now a days, these cities are producing circa 70% of pollutants, even though they only oeeupy 2% of surface of the Earth. Pub- lic utility services cannot meet the demands of unexpected growth. The filthiness in cities causing decreasing of Quality of Life. In this light our research paper is giving more concentration on necessity of ＂ Smart Cities＂, which are the basis for civic centric services. This article is throwing light on Smart Cities and its important roles. The beauty of this manuscript is scribbling ＂Smart Cities＂ concepts in pictorially. Moreover this explains on ＂Barcelona Smart City＂ using lnternet of Things Technologies. It is a good example in urban paradigm shift. Braeelona is like the heaven on the earth with by providing Quality of Life to all urban citizens. The GOD is Interenet of Things.

关键词： smart cities Barcelona City Internet of Things smart mobility open access data

表皮生长因子/HER2抑制剂在非小细胞肺癌患者治疗中的作用综述

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中国肺癌杂志 2010年第4期13卷 363-369页

作者： ramaswamy GOVINDAN 南娟丁燕 Alvin J.Siteman Cancer Center Washington University School of Medicine 天津医科大学总医院天津市肺癌研究所天津市肺癌转移与肿瘤微环境重点实验室

晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)仍然是主要的全球健康问题。尽管可逆性表皮生长因子受体(epidermal growth factor receptor,EGFR)酪氨酸激酶抑制剂厄洛替尼可改善复发与再发NSCLC患者的生存期,但也存在明显的局限... 详细信息

晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)仍然是主要的全球健康问题。尽管可逆性表皮生长因子受体(epidermal growth factor receptor,EGFR)酪氨酸激酶抑制剂厄洛替尼可改善复发与再发NSCLC患者的生存期,但也存在明显的局限性,包括仅对少数患者亚群具有临床疗效、生存率较低及产生耐药性。EGFR和HER2的非可逆性抑制剂是临床开发的新型药物,有可能预防并克服第一代EGFR抑制剂的获得性耐药。

关键词： BIBW 2992 ErbB 厄洛替尼吉非替尼 HKI-272 受体酪氨酸激酶

In vitro cytotoxic studies of red algae Portieria hornemannii and Spyridia fusiformis against Dalton’s lymphoma ascite and Ehrlich ascite carcinoma cell lines

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Journal of Coastal Life Medicine 2016年第12期4卷 949-952页

作者： Murugesan Subbiah bhuvaneswari Sundaresan Thamizh Selvam Natarajan Sivamurugan Vajiravelu Division of Algal Biotechnology and Bionano Technology Post Graduate and Research Department of BotanyPachaiyappa’s CollegeChennai-600030India Central Ayurveda Research Institute for Neuromuscular and Musculo-Skeletal Disorders (CCRASMinistry of AyushGovt.of India)CheruthuruthyThrissurKerala-679531India Post Graduate and Research Department of Chemistry Pachaiyappa’s CollegeChennai 600030India

Objective:To study the in vitro cytotoxic activities of methanol extract of Portieria hornemannii(***)and Spyridia fusiformis(***)using Dalton’s lymphoma ascite and Ehrlich ascite carcinoma cell ***:The effect of cytotoxicity of *** and *** was evaluated with the concentrations(100 to 200μg/mL)and assessed for the antitumour activity *** selected cell lines using Trypan blue ***:The methanol extracts of *** and *** showed potent cytotoxic activity with IC_(50)values of(209.00±0.05)μg/mL and(190.00±0.05)μg/mL against the Dalton’s lymphoma ascite cell line and IC_(50)values of(190.00±0.05)μg/mL and(182.00±0.05)μg/mL against the Ehrlich ascite carcinoma cell line *** vitro cytotoxicity against the tested cancer cell lines showed strong activity by the abnormal activities of algal residue in the normal ***:The methanol solvent residue of red algae(*** and ***)could be a good *** would be a novel marine resource as a antitumor medicine demonstrated by cytotoxic studies that the above marine algae can be a potential candidate sources as antitumor drugs.

关键词： Cytotoxic activity Red algae Dalton’s lymphoma ascite cell line Ehrlich ascite carcinoma cell line

Significance of Impurity Mineral Identification in the Value Addition of Kaolin – A Case Study with Reference to an Acidic Kaolin from India

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Journal of Minerals and Materials Characterization and Engineering 2011年第11期10卷 1007-1025页

作者： S. ramaswamy P. Raghavan National Institute for Interdisciplinary Science and Technology (NIIST) Council of Scientific and Industrial Research (CSIR) Industrial Estate P.O Thiruvananthapuram-695 019 Kerala India

Kaolin or china clay is a versatile industrial mineral with wide technological applications and is abundantly available in India. The major mineral in kaolin is kaolinite (Al2O3.2SiO2.2H2O). The common ancillary / impurity minerals occurring with kaolin include parent rocks like feldspar and mica, quartz, ferruginous, titanoferrous and carbonaceous materials. The most deleterious impurities in kaolin are iron minerals which imparts colour to the white kaolin. Iron exists as oxides, hydroxides, oxy hydroxides, sulphides and carbonates along with iron stained quartz/anatase and mica in kaolin. Kaolin finds extensive applications in paper, paint, rubber, ceramics, plastics etc. One of the highest value additions for kaolin is as pigment in paper and paint industries. The optical properties are important for pigment applications and removal of the iron impurity is very important to improve this property. Extensive research has been carried out on the nature of iron impurities present in kaolin, which leads to the conclusion that iron is present as a part of the kaolinite or ancillary mineral (mica or titania) structure, which can be termed as “structural iron” or as independent iron minerals such as oxides, hydroxides, oxyhydroxides, sulphides and carbonates, which can be termed as “free iron” [1]. The present paper discusses the iron speciation studies carried out on a typical china clay sample collected from Koraput district of Orissa State in the Union of India. Studies have shown that the major impurity mineral species is in “pyritic” (Iron sulphide) form along with other hydroxides, oxyhydoxides and oxides of Iron. Presence of limonite is also observed in the sample. The identification/quantification of the impurity minerals have played a crucial role in the selection / modification and sequentialisation of beneficiation processes and subsequent processing studies have shown that the sample can be value added to ceramic grade.

关键词： Kaolin Impurity minerals Beneficiation Pyrite Value addition