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Sphere-forming corneal cells repopulate dystrophic keratoconic stroma:Implications for potential therapy

World Journal of Stem Cells 2020年第1期12卷 35-54页

作者： Himanshu Wadhwa Salim Ismail Jennifer J McGhee bert van der werf Trevor Sherwin Department of Ophthalmology Faculty of Medical and Health SciencesThe University of AucklandAuckland 1023New Zealand Department of Epidemiology and Biostatistics School of Population HealthFaculty of Medical and Health SciencesThe University of AucklandAuckland 1023New Zealand

BACKGROUND Keratoconus is a degenerative corneal disease characterised by aberrant cell behaviour and loss of matrix that can result in vision *** extracted from peripheral corneas can form stem cell-enriched spheres,which have shown the potential to repopulate the normal peripheral corneal stroma in vitro upon sphere implantation but have not been previously studied in keratoconic *** To investigate the therapeutic potential of stem cell-enriched spheres formed from extracted peripheral human corneal cells when introduced to keratoconic *** Stem cell-enriched spheres were formed from extracts of normal cadaveric human peripheral corneal *** spheres were implanted into incisions created in full thickness and onto the surface of 10μm thin sections of keratoconic and normal stromal tissues in *** sections were used to maximise use of limited keratoconic tissue available for *** cells were stained with Calcein-AM and visualised with stereo and fluorescence microscopy to assess survival and behaviours between the time of implantation day 0 and 14 d(D14)from *** cells in implanted tissues were characterised for stem cell and differentiation markers using immunohistochemistry and droplet digital PCR to assess the potential implications of these characteristics in the use of spheres in keratoconus *** Spheres were successfully implanted into full-thickness central corneal tissue and onto the surface of 10μm thin en face tissue *** observable differences were seen in sphere migration,proliferation or differentiation in keratoconic tissue compared to normal between day 0 and *** stained positively with Calcein-AM up to *** migration increased from day 0 to D14,occurring radially in three dimensions from the sphere and in alignment with tissue *** proliferation marker,EdU,was detected at day *** spheres stained positively for putative stem cell markersΔNp63

关键词： Keratoconus Cell culture Immunohistochemistry Quantitative PCR Digital PCR Spheroid Holoclone Neurosphere Regeneration

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CROP DIVERSITY AND SUSTAINABLE AGRICULTURE:MECHANISMS,DESIGNS AND APPLICATIONS

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Frontiers of Agricultural Science and Engineering 2021年第3期8卷 359-361页

作者： Long LI Wopke van der werf Fusuo ZHANG National Academy of Agriculture Green Development College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Centre for Crop Systems Analysis Wageningen University and ResearchDroevendaalsesteeg 16708 PB Wageningenthe Netherlands

Intensive monoculture agriculture has contributed greatly to global food supply over many decades,but the excessive use of agricultural chemicals(fertilizers,herbicides and pesticides)and intensive cultivation systems has resulted in negative side effects,such as soil erosion,soil degradation,and non-point source pollution[1].To many observers,agriculture looms as a major global threat to nature conservation and *** noted in the Global Biodiversity Outlook 4[2],the drivers associated with food systems and agriculture account for around 70%and 50%of the projected losses by 2050 of terrestrial and freshwater biodiversity,respectively[3].

关键词： Outlook pesticides agriculture

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Fine mapping of multiple interacting quantitative trait loci using combined linkage disequilibrium and linkage information

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第11期8卷 787-791页

作者： LEE Sang Hong van der werf J.H.Julius School of Rural Science and Agriculture University of New England Armidale New South Wales 2351 Australia

Quantitative trait loci (QTL) and their additive, dominance and epistatic effects play a critical role in complex trait variation. It is often infeasible to detect multiple interacting QTL due to main effects often being confounded by interaction effects. Positioning interacting QTL within a small region is even more difficult. We present a variance component approach nested in an empirical Bayesian method, which simultaneously takes into account additive, dominance and epistatic effects due to multiple interacting QTL. The covariance structure used in the variance component approach is based on combined linkage disequilibrium and linkage (LDL) information. In a simulation study where there are complex epistatic interactions between QTL, it is possible to simultaneously fine map interacting QTL using the proposed approach. The present method combined with LDL information can efficiently detect QTL and their dominance and epistatic effects, making it possible to simultaneously fine map main and epistatic QTL.

关键词： Quantitative trait loci (QTL) Combined linkage disequilibrium and linkage (LDL) information Epistatic effects

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A conceptual framework and an empirical test of complementarity and facilitation with respect to phosphorus uptake by plant species mixtures

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Pedosphere 2022年第2期32卷 317-329页

作者： Chunjie LI Thomas W.KUYPER Wopke van der werf Junling ZHANG Haigang LI Fusuo ZHANG Ellis HOFFLAND College of Resources and Environmental Science National Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China Wageningen University Soil Biology GroupP.O.Box 476700 AA WageningenThe Netherlands Wageningen University Centre for Crop Systems AnalysisP.O.Box 4306700 AK WageningenThe Netherlands Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources Key Laboratory of Grassland Resource(IMAU)Ministry of EducationCollege of GrasslandResources and EnvironmentInner Mongolia Agricultural UniversityHohhot 010018China

Plant species have different traits for mobilizing sparingly soluble phosphorus (P) resources,which could potentially lead to overyielding in P uptake by plant species mixtures compared to monocultures due to higher P uptake as a result of resource (P) partitioning and ***,there is circumstantial evidence at best for overyielding as a result of these *** (the outcome) is easily confused with underlying mechanisms because of unclear *** aimed to define a conceptual framework to separate outcome from underlying mechanisms and test it for facilitation and complementarity with respect to P acquisition by three plant species combinations grown on four *** conceptual framework describes both mechanisms of complementarity and facilitation and outcomes (overyielding of mixtures or no overyielding) depending on the competitive ability of the species to uptake the mobilized ***/chickpea mixtures were grown in pots on two calcareous soils mixed with calcium-bound P (CaP) and phytate P (PhyP).Cabbage/faba bean mixtures were grown on both acid and neutral soils mixed with P-coated iron (hydr)oxide (FeP) and ***/maize mixtures were grown on all four *** carboxylate concentration and acid phosphatase activity (mechanisms) as well as plant P uptake and biomass (outcome) were determined for monocultures rhizosphere and species *** of P uptake occurred in millet/chickpea mixtures on one calcareous *** found no indications for P acquisition from different P sources,neither in millet/chickpea,nor in cabbage/faba bean *** and faba bean on the neutral soil differed in rhizosphere acid phosphatase activity and carboxylate concentration,but showed no *** and maize,with similar root exudates,showed overyielding (the observed P uptake being 22%higher than the expected P uptake) on one calcareous *** concluded that although differences in plant physiological tr

关键词： carboxylate intercrop phosphatase phosphorus acquisition phosphorus mobilization phoshorus source rhizosphere root exudation

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Ellipticity on spaces with higher singularities

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Science China Mathematics 2017年第11期60卷 2053-2076页

作者： CHANG der-Chen SCHULZE bert-Wolfgang Department of Mathematics and Statistics Georgetown University Department of Mathematics Fu Jen Catholic University Institute of Mathematics University of Potsdam

We study corner-degenerate pseudo-differential operators of any singularity order and develop ellipticity based on the principal symbolic hierarchy, associated with the stratification of the underlying space. We construct parametrices within the calculus and discuss the aspect of additional trace and potential conditions along lower-dimensional strata.

关键词： pseudo-differential operators operator-valued symbols Fourier and Mellin transforms

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Intercropping enables a sustainable intensification of agriculture

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Frontiers of Agricultural Science and Engineering 2020年第3期7卷 254-256页

作者： Wopke van der werf Chunjie LI Wen-Feng CONG Fusuo ZHANG Plant Sciences Crop Systems Analysis GroupWageningen University and Research6708PB Wageningenthe Netherlands College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China Soil Biology Group Wageningen University and Research6700AA Wageningenthe Netherlands

Intercropping is the cultivation of more than one crop species on a single parcel of land. Intercropping seeks toexploit species complementarities to capture more of the available light, water and nutrient resources, and thusincrease combined crop yield[1]. Intercropping is well known in China, where smallholder farmers practice a greatdiversity of species combinations to increase their yields[2]. Figure 1 illustrates intercropping as done by a farmer inGansu Province, China, who chose to combine wheat, soybean and maize. This three-way combination offersseveral species complementarities. First, the growing period of wheat ends earlier than that of soybean and maize,so the soybean and maize can use all the light, water and nutrient resources of the land after wheat harvest. With thewheat covering only around half of the area, the plants will still produce about 70% of the normal yield for wheatgrown as a sole crop, because the wheat has virtually no competition for resources early on, resulting in greatercapture of light, water and nutrient resources in the intercrop than in a sole crop[3]. Furthermore, soybean and maizehave a complementarity for nitrogen acquisition, with maize requiring nitrogen from soil, but soybean being able to fixit from the air. Therefore, this combination can reduce fertilizer requirements.

关键词： soybean Figure enable

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Resource use efficiency, ecological intensification and sustainability of intercropping systems

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Journal of Integrative Agriculture 2015年第8期14卷 1542-1550页

作者： MAO Li-li ZHANG Li-zhen ZHANG Si-ping Jochem B Evers Wopke van der werf WANG Jing-jing SUN Hong-quan SU Zhi-cheng Huub Spiertz College of Agronomy and Biotechnology China Agricultural University College of Agricultural Resources and Environmental Sciences China Agricultural University Institute of Cotton Research Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology Centre for Crop Systems Analysis (CSA) Department of Plant Sciences Wageningen University China Institute of Water Resources and Hydropower Research

The rapidly growing demand for food, feed and fuel requires further improvements of land and water management, crop productivity and resource-use *** field experimentation and crop growth modelling during the past five decades made a great leap forward in the understanding of factors that determine actual and potential yields of *** research field of production ecology developed concepts to integrate biological and biophysical processes with the aim to explore crop growth potential in contrasting *** understand the potential of more complex systems（multi-cropping and intercropping） we need an agro-ecosystem approach that integrates knowledge derived from various disciplines： agronomy, crop physiology, crop ecology, and environmental sciences（soil, water and climate）.Adaptation of cropping systems to climate change and a better tolerance to biotic and abiotic stresses by genetic improvement and by managing diverse cropping systems in a sustainable way will be of key importance in food *** accelerate sustainable intensification of agricultural production, it is required to develop intercropping systems that are highly productive and stable under conditions with abiotic constraints（water, nutrients and weather）.Strategies to achieve sustainable intensification include developing tools to evaluate crop growth potential under more extreme climatic conditions and introducing new crops and cropping systems that are more productive and robust under conditions with abiotic *** paper presents some examples of sustainable intensification management of intercropping systems that proved to be tolerant to extreme climate conditions.

关键词： abiotic stress farming systems over-yielding resource use efficiency sustainability

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COMPARING PERFORMANCE OF CROP SPECIES MIXTURES AND PURE STANDS

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Frontiers of Agricultural Science and Engineering 2021年第3期8卷 481-489页

作者： Wopke van der werf Lizhen ZHANG Chunjie LI Ping CHEN Chen FENG Zhan XU Chaochun ZHANG Chunfeng GU Lammert BASTIAANS David MAKOWSKI TjeerdJan STOMPH Wageningen University Centre for Crop Systems Analysis6700 AK Wageningenthe Netherlands College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193China College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China College of Agronomy/Sichuan Engineering Research Center for Crop Strip Intercropping System/Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture and Rural Affairs of ChinaChengdu 611130China Tillage and Cultivation Research Institute Liaoning Academy of Agricultural SciencesShenyang 110161China INRAe AgroParisTechUniversitéParis-SaclayUnit Applied Mathematics and Computer Science(UMR MIA 518)16 rue Claude Bernard F-75231 ParisFrance

Intercropping is the planned cultivation of species mixtures on agricultural *** has many attributes that make it attractive for developing a more sustainable agriculture,such as high yield,high resource use efficiency,lower input requirements,natural suppression of pests,pathogens and weeds,and building a soil with more organic carbon and *** is needed which species combinations perform best under different circumstances and which management is suitable to bring out the best from intercropping in a given production *** literature is replete with case studies on intercropping from across the globe,but evidence synthesis is needed to make this information ***-analysis requires a careful choice of metric that is appropriate for answering the question at hand,and which lends itself for a robust *** paper reviews some metrics that may be used in the quantitative synthesis of literature data on intercropping.

关键词： intercropping species mixtures metaanalysis metrics indicators

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LIGHT INTERCEPTION AND USE EFFICIENCY DIFFER WITH MAIZE PLANT DENSITY IN MAIZE-PEANUT INTERCROPPING

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Frontiers of Agricultural Science and Engineering 2021年第3期8卷 432-446页

作者： Qi WANG Zhanxiang SUN Wei BAI Dongsheng ZHANG Yue ZHANG Ruonan WANG Wopke van der werf Jochem B.EVERS Tjeerd-Jan STOMPH Jianping GUO Lizhen ZHANG State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological ScienceBeijing 100081China China Agricultural University College of Agricultural Resources and Environmental SciencesBeijing 100193China Wageningen University Centre for Crop Systems Analysis(CSA)Droevendaalsesteeg 16708 PB Wageningenthe Netherlands Tillage and Cultivation Research Institute Liaoning Academy of Agricultural SciencesShenyang 110161China Shanxi Agricultural University College of AgricultureTaigu 030800China

Intercropping increases crop yields by optimizing light interception and/or use *** intercropping combinations and metrics have been reported,the effects of plant density on light use are not well ***,we examined the light interception and use efficiency in maize-peanut intercropping with different maize plant densities in two row configurations in semiarid dryland agriculture over a two-year *** field experiment comprised four cropping systems,i.e.,monocropped maize,monocropped peanut,maize-peanut intercropping with two rows of maize and four rows of peanut,intercropping with four rows of maize and four rows of peanut,and three maize plant densities(3.0,4.5 and 6.0 plants m^(-1) row)in both monocropped and intercropping *** mean total light interception in intercropping across years and densities was 779 MJ·m^(-2),5.5%higher than in monocropped peanut(737 MJ·m^(-2))and 7.6%lower than in monocropped maize(843 MJ·m^(-2)).Increasing maize density increased light interception in monocropped maize but did not affect the total light interception in the *** years the LUE of maize was 2.9 g·MJ–1 and was not affected by cropping system but increased with maize plant *** LUE of peanut was enhanced in intercropping,especially in a wetter *** yield advantage of maize-peanut intercropping resulted mainly from the LUE of *** results will help to optimize agronomic management and system design and provide evidence for system level light use efficiency in intercropping.

关键词： dryland agriculture light interception light use efficiency maize-peanut intercropping semiarid

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昆虫杆状病毒流行病模拟模型及Java模拟软件(英文)

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环境昆虫学报 2011年第3期33卷 283-301页

作者：张文军 Wopke van der werf 庞义中山大学生命科学学院广州510275 荷兰王国瓦哈宁根大学

研究昆虫杆状病毒流行病模拟模型,对确定基因工程改造杆状病毒的主攻方向,明确病毒病田间流行的机制与关键因素,以及制定生物防治策略,均具有重要的理论与实践意义。本研究研制了用于昆虫杆状病毒流行病模拟的数学模型和Java模拟软件,... 详细信息

研究昆虫杆状病毒流行病模拟模型,对确定基因工程改造杆状病毒的主攻方向,明确病毒病田间流行的机制与关键因素,以及制定生物防治策略,均具有重要的理论与实践意义。本研究研制了用于昆虫杆状病毒流行病模拟的数学模型和Java模拟软件,该模型包括描述种群动态的一个微分方程组,描述气温变化、作物生长及病毒动态的若干模型等。模拟软件用工具包JDK和JavaScript开发,由主计算类、图形类、结果显示类、参数输入界面类、警告信息类、主页、用户指南页、版权页、计数页等组成。在参数文件中编入有关作物、病毒、害虫等方面的参数,输入初始的各龄健康、染病虫量、叶面积、病毒密度等,运行后可输出各龄健康、染病及病死的虫量,作物损失,病毒积累等动态,以及图形等等。该模型适用于各种杆状病毒,各种有叶作物,各种食叶性的全变态昆虫。应用该模拟模型,对温度、病毒施用虫龄、病毒施用时间、病毒施用剂量等进行了灵敏度分析,得到了一些重要结论。

关键词：昆虫杆状病毒流行病模拟模型灵敏度分析 Java软件

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