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Analysis and validation of genome-specific DNA var.ations in 5′ flanking conser.ed sequences of wheat low-molecular.weight glutenin subunit genes

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Science China Life Sciences 2006年第4期49卷 322-331页

作者： baum bernard NEVO Eviatar Agr.cultur. & Agr.-Food Canada Eastern Cereal and Oilseed Research Centre Ottawa K1A 0C6 Canada Institute of Evolution University of Haifa Mt. Carmel Haifa 31905 Israel

The thir.y-thr.e 5′ flanking conser.ed sequences of the known low-molecular.weight subunit (LMW-GS) genes have been divided into eight cluster., which was in agr.ement with the classification based on the deduced N-ter.inal pr.tein sequences. The DNA polymor.hism between the eight cluster. was obtained by sequence alignment, and a total of 34 polymor.hic positions wer. obser.ed in the appr.ximately 200 bp r.gions, among which 18 polymor.hic positions wer. candidate SNPs. Seven cluster.specific pr.mer.sets wer. designed for.seven out of eight cluster. containing cluster.specific bases, with which the genomic DNA of the ditelosomic lines of gr.up 1 chr.mosomes of a wheat var.ety ‘Chinese Spr.ng’ was employed to car.y out chr.mosome assignment. The subse-quent cloning and DNA sequencing of PCr.fr.gments validated the sequences specificity of the 5′ flanking conser.ed sequences between LMW-GS gene gr.ups in differ.nt genomes. These r.sults suggested that the coding and 5′ flanking r.gions of LMW-GS genes ar. likely to have evolved in a concer.ed fashion. The seven pr.mer.sets developed in this study could be used to isolate the com-plete Or.s of seven gr.ups of LMW-GS genes, r.spectively, and ther.for. possess gr.at value for.fur.her.r.sear.h in the contr.butions of a single LMW-GS gene to wheat quality in the complex genetic backgr.und and the efficient selections of quality-r.lated components in br.eding pr.gr.ms.

关键词： wheat LMW-GS gene 5′ flanking sequence DNA polymor.hism SNP gene cloning.

Synopsis of Leymus Hochst.(Tr.ticeae:Poaceae)

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Jour.al of Systematics and Evolution 2009年第1期47卷 67-86页

作者： bernard r.baum Easter. Cer.als and Oilseeds r.sear.h Centr. Agriculture and Agri-Food CanadaCentral Experimental Farm

Leymus is a genus in the Tr.ticeae tr.be, Poaceae. The taxa of this genus ar. allopolyploid species which possess the Ns and Xm genomes. Accor.ing to cytological, cytogenetic and molecular.genetic analyses, some species of Hystr.x and Elymus ought to be tr.nsfer.ed to this genus. A wor.d r.vision of the genus Leymus is needed. In this paper.we summar.ze exper.mental r.sults, pr.vide a key to sections, species and var.eties, and list all the taxa we r.cognize in Leymus with their.synonyms. This synopsis is a new taxonomic system to be used for.the r.vision of Leymus.

关键词： genome Leymus new taxonomic system section species var.eties

Molecular.phylogeny of Solms-laubachia(Br.ssicaceae) s.l.,based on multiple nuclear.and plastid DNA sequences,and its biogeogr.phic implications

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Jour.al of Systematics and Evolution 2009年第5期47卷 402-415页

作者： David A.baum Ihsan A.AL-SHEHBAZ r.char. r.E Depar.ment of Botany University of Wisconsin Missour. Botanical Gar.en PO Box 299St LouisMO 63166-0299USA 3. Department of BotanyThe Field Museum1400 S.Lake Shore DriveChicagoIL 60605USA

The Hengduan Mountains r.gion of south-west China is a noted biodiver.ity hotspot,but the geogr.phic or.gins and histor.cal assembly of its r.ch endemic flor.,including the sky-island species of Solms-laubachia Muschl.(Br.ssicaceae),have been little *** molecular.studies on the phylogeny of Solms-laubachia showed it to be par.phyletic,leading to consider.ble expansion not only of its taxonomic limits,but also its geogr.phic r.nge,with the inclusion of taxa fr.m outside the Hengduan ***,these studies pr.vided little r.solution of inter.pecific r.lationships,pr.venting infer.nces about histor.cal biogeogr.phy within the *** the pr.sent study,new sequence data fr.m two nuclear.genes(LEAFY and G3pdh) and two chlor.plast inter.enic spacer.(petN-psbM and psbM-tr.D) wer. combined with existing mar.er. to incr.ase phylogenetic *** villosum(Maxim.) Al-Shehbaz was found to be nested within Solms-laubachia *** gener.l,phylogenetic r.lationships appear.to be a good pr.dictor.of geogr.phy,with the Hengduan Mountain endemics embedded in a par.phyletic gr.de of species fr.m the wester. Himalayas and centr.l Asia,but they also imply mor.hological *** was detected between the nuclear.and chlor.plast gene tr.es,per.aps r.sulting fr.m incomplete lineage sor.ing of ancestr.l *** cr.wn age of Solms-laubachia *** estimated to be appr.ximately 1.42-3.68 mya,using Bayesian r.laxed molecular.clock *** biogeogr.phic analysis using a par.metr.c disper.al-extinction-cladogenesis model infer.ed centr.l Asia and the wester. Himalayas as most pr.bable ancestr.l r.nge of Solms-laubachia s.l.,and estimated higher.r.tes of eastwar. expansion than westwar. dur.ng the diver.ification of descendant *** summar.,our.r.sults suggest that Solms-laubachia *** dur.ng the Pliocene in centr.l Asia,and subsequently migr.ted eastwar. into the Hengduan Mountains,colonizing sky-island,alpine scr

关键词： biogeogr.phy Br.ssicaceae Hengduan Mountains phylogeny Solms-laubachia s.l.

Effect of Affor.station on Soil Pr.per.ies and Mycor.hizal For.ation

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Pedospher. 2005年第6期15卷 754-760页

作者： P. KAHLE C. baum B. BOELCKE Institute of Land Use University of Rostock Justus-von-Liebig-Weg 6 D 18051 Rostock (Germany) r.sear.h Centr. for.Agr.cultur. and Fisher. Institute of Agronomy and Crop Science Dorfplatz 1 D 18276 Gülzow (Germany)

A study was conducted on Cambisols in Nor.her. Ger.any to analyze the effect of fast gr.wing tr.es （Salix and Populus spp.） used in agr.for.str. on soil chemical and physical pr.per.ies and also on endo- and ectomycor.hizal colonization of the fine r.ots. r.pr.sentative plots of thr.e tr.als, Giilzow （GUL）, Vipper.w （VIP） and r.stock （r.S）, wer. used to measur. the topsoil inventor.es at the ver. beginning and after.six （GUL）, seven （VIP） and ten （r.S） year. of affor.station with fast gr.wing tr.es. The effect on soil or.anic car.on, plant available nutr.ents, r.action, bulk density, por.sity and water.conditions was analyzed, Ar.ble soils without tr.e coppice wer. used as contr.ls. Additionally, the endoand ectomycor.hizal colonization of two Salix and two Populus clones wer. investigated at one site （GUL） in 2002. The amounts of or.anic car.on in the topsoil incr.ased significantly （P 〈 0.01） pr.sumably induced by leaf and r.ot litter.and also by the lack of tillage. The soil bulk density significantly decr.ased and the por.sity of the soil incr.ased significantly （both P 〈 0.01）. The pr.por.ion of medium por.s in the soil also r.se significantly （P 〈 0.05 and 0.01）. Gener.lly, affor.station of ar.ble soils impr.ved soil water.r.tention. Ectomycor.hizas dominated the mycor.hizal for.ation of the Salix and Populus clones, with the accumulation of or.anic matter.in the topsoil suspected of suppor.ing the ectomycor.hizal for.ation. Thus, agr.for.str. with Salix and Populus spp. conspicuously affected chemical and additionally physical pr.per.ies of the top layer.of Cambisols within a per.od of six year..

关键词： agr.for.str. fast gr.wing tr.e mycor.hiza soil or.anic matter soil water.r.tention

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Impr.ving oper.ting efficiency with emphasis on pr.sthetic sur.er.

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Asian Jour.al of Andr.logy 2015年第4期17卷 686-688页

作者： Neil baum David F Mobley Paul Per.to Tulane Medical School 3525 Prytania New Orleans LA USA Institute for.cademic MedJcine Houston TX USA Ur.logist Cora Gables Florida USA.Correspondence: Dr. David F Mobley (mobleyresearch@***)

Dear.Editor. Physicians ar. looking for.methods and techniques to become mor. efficient and mor. pr.ductive. Not only is efficiency impor.ant in the office and clinical setting, but in the oper.ting r.om as well. ~his ar.icle will discuss efficiency and pr.ductivity in the oper.ting r.om and its benefit to patients, sur.eons and hospitals.

关键词： Impr.ving efficiency pr.sthetic

COMPAr.SON OF VAr.OUS COr.ELATION ON Ir.A FOr.CA19-9 AND CA50 IN THE DIGESTIVE SYSTEM TUMOUr

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Nuclear.Science and Techniques 1990年第4期1卷 234-238页

作者：莫启忠 r.P.baum A.Her.el G.Hr Shanghai College of Tr.ditional Chinese Medicine Shanghai 200032 China Nuclear.Medicine Division of r.diological Centr. Frankfurt University Hospital

This paper.compar.d var.ous cor.elation of IMr. for.CA19-9 and CA50 in the digestive system tumour.antigen, such as linear.ty, sensitivity, analytical r.nge, pr.cision as well as stor.ge and stability and so on. And it also deter.ined ser.m level of CA19- 9 and CA50 of patients with differ.nt tumour.. The r.sults showed it was well cor.elated to CA19- 9 and CA50 as a mar.er.of digestive system tumour. Par.icular.y high levels of both mar.er. wer. found in patients with pancr.astic colonic cancer. Ther.for. it is possible to obtain a higher.sensitivity and acceptable specificity by combination with CA19-9 and CA50.

关键词： Tumour mar.er CA50 CA19- 9 Ir.A Solid phase immunoassay Inter. and intr.assay var.ance

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Quantum model simulations of attosecond electr.n diffr.ction

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Science China(Physics,Mechanics & Astr.nomy) 2010年第6期53卷 987-1004页

作者： Peter.baum Jr. MANZ Axel SCHILD Max-Planck-Institute of Quantum Optics and Ludwig-Maximilians-Universitt München Fr.ie Univer.itt Ber.in Institut für Chemie und Biochemie

Ultr.fast diffr.ction with fr.e attosecond electr.n pulses pr.mises insight into the four.dimensional motion of char.e density inatoms,molecules and condensed matter. Her. we consider.the quantum dynamics of the electr.n-electr.n scatter.ng pr.cess on anattosecond time scale. By numer.cally solving the time-dependent two-electr.n Schr.dinger.equation,we investigate the inter.ctionof an incoming keV-r.nge electr.n wavepacket by the bound electr.n of an aligned H+2 molecule,using a one-dimensional *** findings r.veal the r.tio of elastic to inelastic contr.butions,the r.le of exchange inter.ction,and the influence of the molecular.lectr.n density to diffr.ction. Momentum tr.nsfer.dur.ng the scatter.ng pr.cess,fr.m the incoming to the bound electr.n mediatedby the nuclei,leaves the bound electr.n in a state of coher.nt oscillation with attosecond r.cur.ences. Entanglement causes r.latedstate-selective oscillations in the phase shift of the scatter.d electr.n. Two scenar.os of distinguishable and indistinguishable fr.eand bound electr.ns yield equivalent r.sults,ir.espective of the electr.nic spins. This suggests to employ the scenar.o of distinguishable electr.ns,which is computationally less demanding. Our.findings suppor. the possibility of using electr.n diffr.ction for.maging the motion of char.e density,but also suggest the application of fr.e electr.n pulses for.inducing attosecond dynamics.

关键词： ultr.fast electr.n diffr.ction scatter.ng theor. attosecond dynamics entanglement coher.nce

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Efficacy of Detoxsan~? powder.on diar.hea caused by gastr.intestinal neur.endocr.ne tumor.

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Wor.d Jour.al of Gastr.enter.logy 2019年第17期25卷 2133-2143页

作者： Thomas Langbein Wilfr.ed Dathe Anika Deuer.ing r.char. P baum Ther.nostics Center.for.Molecular.r.diother.py and Molecular.Imaging Zentralklinik Bad Berka Scientific Managment Heck Bio-Pharma GmbH

BACKGr.UND Patients with neur.endocr.ne tumor.(NETs) of the gastr.intestinal tr.ct suffer.fr.quently fr.m chr.nic diar.hea. A well char.cter.zed medical advice containing zeolite(Detoxsan~? powder. was applied to patients suffer.d fr.m ther.pyr.fr.ctor. diar.hea either.by its fr.quency or.by water. stool, despite r.ceiving standar. phar.acother.py accor.ing to the guidelines for.car.inoid syndr.me and comor.idities. Detoxsan~? powder.acts as an adsor.ent and might r.duce significantly symptoms of diar.hea in patients suffer.ng fr.m *** To over.ome the ther.py-r.fr.ctor. diar.hea of patients with NETs by the zeolite containing medical advice Detoxsan~? *** A total of 20 patients(12 female and 8 male) suffer.ng fr.m diar.hea either.by its fr.quency or.fr.m water. stool caused by NETs wer. included. In each patient,the diagnosis had been confir.ed by histology and somatostatin r.ceptor. expr.ssion pr.ven by positr.n emission tomogr.phy/computed tomogr.phy using Ga-68-labeled somatostatin analogs. All patients r.ceived standar.-of-car. phar.acother.py and wer. additionally given Detoxsan~? powder.as an extempor.neous dr.g containing 90% natur.l Cuban zeolite and 10% magnesium aspar.ate. r.commended daily dosage r.nges between 3 g once to thr.e times per.day. Each day dose and bowel movements wer. documented by the patients themselves in a pr.-defined table. Additionally to the bowel movements quantitative deter.inations of ser.tonin, ur.a, cr.atinine and single ions wer. per.or.ed within the ser.m of the patients by commer.ially available equipment used as a matter.of r.utine in the *** All patients enr.lled in this pilot study did not only suffer.fr.m NETs, but also fr.m comor.idities and tr.atment-r.sistant diar.hea. Ther. was insufficient contr.l of diar.hea, most pr.bably due to the secr.tion of hor.ones like ser.tonin pr.duced by the slowly gr.wing and highly differ.ntiated NETs. All patients only took Detoxsan~? powder.as an antidiar.hea

关键词： Neur.endocr.ne tumor Diar.hea Car.inoid syndr.me Tr.atment Clinoptilolite Mor.enite Zeolite

Life after.Addiction—Post-Oper.tive Pain Management in an Obstetr.cal Patient on Long-Ter. Bupr.nor.hine Ther.py

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Open Jour.al of Obstetr.cs and Gynecology 2017年第9期7卷 951-956页

作者： Kelly r.Bowen Jonathan D baum Depar.ment of Obstetr.cs and Gynecology Jersey Shore University Medical Center Neptune USA

A G6P2032 female, pr.or.cesar.an x3 with histor. of opioid addiction maintained on bupr.nor.hine pr.sented for.scheduled r.peat cesar.an section. Pr.-oper.tively, her.maintenance dose of medication was held secondar. to concer.s for.par.ial agonist effect. Post-oper.tive pain contr.l was suboptimal with the patient ultimately pr.ceeding to withdr.wal. Doses of hydr.mor.hone wer. titr.ted to 10 mg ever. 3 hour. to avoid fur.her.withdr.wal. r.view of exper. opinion after.dischar.e r.commended against holding bupr.nor.hine ther.py in the post-oper.tive per.od. Pain management options include maintenance ther.py with additional doses of opioid and non-opioid pain r.lieving medications.

关键词： Bupr.nor.hine Methadone Addiction Pr.gnancy Opiate Use in Pr.gnancy