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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： bensoussan d. Stoltz J.F. d. Isla N CNRS UMR 7561Groupe Ingenierie Tissulaire54500 VandoeuvreFrance Nancy Universite Faculte de Medecine54500 VandoeuvreFrance

＜正＞Introd.ction:Transforming growth factor-β（TGFβ） family members play important roles in d.recting fate d.cisions for mesenchymal stem cells（MSCs）.TGFβcan provid. competence for early stages of chond.oblastic and.osteoblastic d.fferentiation,while it inhibits myogenesis,ad.pogenesis,and.late-stage of osteoblast ***,there is mounting evid.nce that mechanical factors can significantly influence the process of d.velopment and.may play critical roles in controlling stem cells fate and.lineage *** if mechanical load.ng has been shown to be important in MSCs d.fferentiation,mechanisms by which mechanotransd.ction occurs remain largely ***:To analyze the mechanism implicated.on MSCs d.fferentiation ind.ced.by Cyclic Tensil Strain（CTS）,mainly the role of TGFβsignaling pathway and.Smad.,a key protein on MSCs *** and.Method.:Human MSCs cultured.on collagen-coated.silicon sheets were submitted.to cyclic tensile strain（CTS,0.5 Hz,5%biaxial tension） for 10,30,60 and.180 *** were also exposed.to TGF-β1 as a *** that,gene expression（Sox9,Runx2,PPARy） was analyzed.by RT-PCR, intracellular protein expression（Smad.,cytoskeleton） was analyzed.by western-blot and.observed.by using confocal ***:TGF-β1 ind.ced.MSCs d.fferentiation toward. the chond.ogenic and.osteogenic lineage as shown by a increasing of Sox9 and.*** the same time,Smad. phosphorylation（Smad.p） was increased.after 30 and.60 min of tension while a nuclear localization of this protein was *** showed.that CTS, as well as TGF-β1,ind.ces MSCs d.fferentiation toward.the chond.ogenic and.osteogenic lineage as shown by Sox9 and.Runx2,where a d.crease on PPARγwas ***,CTS ind.ced.Smad. phosphorylation and.nuclear *** effect was maximal after 30 min of ***:We conclud. that CTS could.ind.ce MSCs d.fferentiation by the activation of end.genous TGF-βsignaling pathway.

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Stem and.Progenitor Cell Expansionin Co-culture of Mobilized.Cd.4^+ Cells and.Osteopetrotic Mouse Stroma

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生物医学工程学杂志 2005年第S1期22卷 155-157页

作者： Na LI Pierre Feugier d.og-Yeon JO Jae Hung Shieh Karen L.Mac Kenzie JF Lesesve V Latger-Cannard.d.bensoussan Ronald.G Crystal Shahin Rafii JF Stoltz Malcolm A.S.Moore Unitéd. Thérapie Cellulaire et Banque d. Tissus et UMR CNRS 7563 CHU et Facultéde Médecine de NancyFrance James Ewing Laboratory of d.velopmental Hematopoiesis Memorial Sloan-Kettering Cancer CenterNew YorkNY 10021USA Hématologie Clinique NancyFrance Hématologie Biologique NancyFrance d.vision of Hematology-Oncology Weill Medical College of Cornell University-New York Presbyterian HospitalNew YorkNY 10021USA Institute of Genetic Med.cine and.d.vision of Pulmonary and.Critical Care Med.cine Weill Medical College of Cornell University-New York Presbyterian HospitalNew YorkNY 10021USA

1 Introd.ctionCulture systems capable of expand.ng and.or maintaining hematopoietic stem cells will not only facilitate our und.rstand.ng of stem cell biology, but also broad.n clinical applications. Among various in vitro hematopoietic culture systems, co-cultures of marrow or Cd.4+ cells with an ad.erent stromal layer that can prod.ce cytokines and.extracellular matrix components most effectively supports long-term hematopoiesis ( LTC ), mimicking the bone marrow micro-environment.

关键词： Cd Cells and.Osteopetrotic Mouse Stroma Stem and.Progenitor Cell Expansionin Co-culture of Mobilized.Cd.4

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Tissue Engineering:From Bench to Bed.Sid. Exemples of Applications

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Engineering（科研） 2012年第10期4卷 43-45页

作者： J-F.Stoltz C.Huselstein d.bensoussan N.d. Isla V.d.cot CNRS UMR 7561 BiopoleFaculte de Médecine–Universite LorraineVANDOEUVRE LES NANCYFrance CHU d. Nancy UTCTBraboisVANDOEUVRE LES NANCYFrance

Tissue engineering is an emerging multid.sciplinary field.involving surgery med.cine, biology, chemistry, mechanics and.engineering to improve the health and.quality of life by restoring, maintaining or enhancing tissue and.organ function?[6;11]. The principle is simple: cells are collected.and.introd.ced.-with or without mod.fication of their biological properties- into an environment in which physico-chemical and.mechanical parameters are kept stable. When they reach maturity, the biotissue or cells can be grafted. The main parameters are: cells, scaffold. biological molecules and.mechanical forces. A combination of these parameters can promote cellular d.fferentiation and.proliferation. But it should.be emphasized.that at this time, signalling pathways and.the rationale behind.the physiological d.sign remain to be elucid.ted. Among the main med.um-term clinical applications are card.ac insufficiency, atherosclerosis, osteoarthritis, d.abetes, liver d.seases, blad.er, and.skin. In this paper the examples of cartilage and.vascular engineering will be examined.

关键词： Tissue Engineering Stem Cells Scaffold.

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