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Age-Triggered and Dark-Induced Leaf Senescence Require the bHLH Transcription Factors PIF3, 4, and 5

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Molecular Plant 2014年第12期7卷 1776-1787页

作者： Yi Song Chuangwei Yang Shan Gao Wei Zhang Lin Li benke kuai State Key Laboratory of Genetic Engineering Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433People's Republic of China National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai) Institute of PlantPhysiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 People's Republic of China

Leaf senescence can be triggered and promoted by a large number of developmental and environmental fac- tors. Numerous lines of evidence have suggested an involvement of phytochromes in the regulation of leaf senescence, but the related signaling pathways and physiological mechanisms are poorly understood. In this study, we initially identi- fied phytochrome-interacting factors （PIFs） 3, 4, and 5 as putative mediators of leaf senescence. Mutations of the PIF genes resulted in a significantly enhanced leaf longevity in age-triggered and dark-induced senescence, whereas overexpressions of these genes accelerated age-triggered and dark-induced senescence in Arabidopsis. Consistently, loss-of-function of PIF4 attenuated dark-induced transcriptional changes associated with chloroplast deterioration and reactive oxygen species （ROS） generation. ChlP-PCR and DualoLuciferase assays demonstrated that PIF4 can activate chlorophyll degradation regulatory gene NYE1 and repress chloroplast activity maintainer gene GLK2 by binding to their promoter regions. Finally, dark-induced ethylene biosynthesis and ethylene-induced senescence were both dampened in pif4, suggesting the involvement of PIF4 in both ethylene biosynthesis and signaling pathway. Our study provides evidence that PIF3, 4, and 5 are novel positive senes- cence mediators and gains an insight into the mechanism of light signaling involved in the regulation of leaf senescence.

关键词： phytochrome-interacting factor (PIF) leaf senescence chloroplast deterioration NYEI/SGR1 GLK2 ethylene.

Reverse Genetic Identification of CRN1 and its Distinctive Role in Chlorophyll Degradation in Arabidopsis

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Journal of Integrative Plant Biology 2010年第5期52卷 496-504页

作者： Guodong Ren Qian Zhou Shouxin Wu Yufan Zhang Lingang Zhang Jirong Huang Zhenfei Sun benke kuai State Key Laboratory of Genetic Engineering Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433China National Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institute for Biological SciencesChinese Academy of Sciences Shanghai 200032 China

Recent identification of NYE1/SGR1 brought up a new era for the exploration of the regulatory mechanism of Chlorophyll （Chl） degradation. Cluster analysis of senescence associated genes with putative chloroplast targeting sequences revealed several genes sharing a similar expression pattern with NYE1. Further characterization of available T-DNA insertion lines led to the discovery of a novel stay-green gene CRN1 （Co-regulated with NYE1）. Chl breakdown was significantly restrained in crnl-1 under diversified senescence scenarios, which is comparable with that in acdl-20, but much more severe than that in nyel- 1. Notably, various Chl binding proteins, especially trimeric LHCP II, were markedly retained in crnl-1 four days after dark-treatment, possibly due to a lesion in disassociation of protein-pigment complex. Nevertheless, the photochemical efficiency of PSII in crnl-1 declined, even more rapidly, two days after dark-treatment, compared to those in Col-0 and nyel-1. Our results suggest that CRN1 plays a crucial role in Chl degradation, and that loss of its function produces various side-effects, including those on the breakdown of Ch-protein complex and the maintenance of the residual photosynthetic capability during leaf senescence.

关键词： Senescence Degradation Genes Complexing Darkness Available days Proteins Protein products

REF6 promotes lateral root formation through de-repression of PIN1/3/7 genes

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Journal of Integrative Plant Biology 2019年第4期61卷 383-387页

作者： Xiaolei Wang Jiong Gao Shan Gao Zhongpeng Li benke kuai Guodong Ren State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant Biology School of Life Sciences Fudan University School of Life Sciences Qilu Normal University

The H3K27 methyltransferase CLF inhibits lateral root(LR) formation through depositing the repressive H3K27me3 mark to the chromatin of PIN1, a key polar auxin transporter gene. Here, we show that the H3K27me3 demethylase REF6 promotes lateral root primordium initiation and LR emergence. REF6 directly binds to the chromatin of PIN1/3/7. Dysfunction in REF6 results in increased levels of H3K27me3 on PIN1/3/7 and suppressed expression of PIN genes. Genetic analysis of the clf ref6 double mutant revealed an antagonistic action between CLF and REF6, in terms of LR *** findings indicate that H3K27 methylation and demethylation activities are likely coordinated to ensure proper LR organogenesis.

关键词： REF6 PIN1/3/7 genes^FA

ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis

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Molecular Plant 2016年第9期9卷 1272-1285页

作者： Shan Gao Jiong Gao Xiaoyu Zhu Yi Song Zhongpeng Li Guodong Ren Xin Zhou benke kuai State Key Laboratory of Genetic Engineering School of Life Sciences Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan University Shanghai 200438 China These authors contributed equally to this article.

Chlorophyll （Chl） degradation is an integral process of leaf senescence, and NYE1/SGR1 has been demonstrated as a key regulator of Chl catabolism in diverse plant species. In this study, using yeast one-hybrid screening, we identified three abscisic acid （ABA）-responsive element （ABRE）-binding transcription factors, ABF2 （AREB1）, ABF3, and ABF4 （AREB2）, as the putative binding proteins of the NYE1 promoter. Through the transactivation analysis, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrated that ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo. ABA is a positive regulator of leaf senescence, and exogenously applied ABA can accelerate Chl degradation. The triple mutant of the ABFs, abf2abf3abf4, as well as two ABA-insensitive mutants, abil-1 and snrk2.2/2.3/2.6, exhibited stay-green phenotypes after ABA treatment, along with decreased induction of NYE1 and NYE2 expression. In contrast, overexpression of ABF4 accelerated Chl degradation upon ABA treatment. Interestingly, ABF2/3/4 could also activate the expression of two Chl catabolic enzyme genes, PAO and NYCl, by directly binding to their promoters. In addition, abf2abf3abf4 exhibited a functional stay-green phenotype, and senescence-associated genes （SAGs）, such as SAG29 （SWEET15）, might be directly regulated by the ABFs. Taken together, our results suggest that ABF2, ABF3, and ABF4 likely act as key regulators in mediating ABA-triggered Chl degradation and leaf senescence in general in Arabidopsis.

关键词： abscisic acid ABF transcription factor chlorophyll degradation leaf senescence Arabidopsisthaliana

The NPR1-WRKY46-WRKY6 signaling cascade mediates probenazole/salicylic acid-elicited leaf senescence in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2021年第5期63卷 924-936页

作者： Dingyu Zhang Zheng Zhu Jiong Gao Xin Zhou Shuai Zhu Xiaoyan Wang Xiaolei Wang Guodong Ren benke kuai State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China Institute of Neurology Huashan HospitalFudan UniversityShanghai 200040China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan UniversityShanghai 200438China

Endogenous salicylic acid(SA) regulates leaf senescence, but the underlying mechanism remains largely unexplored. The exogenous application of SA to living plants is not efficient for inducing leaf senescence. By taking advantage of probenazole(PBZ)-inducedbiosynthesisof endogenous SA, we previously established a chemical inducible leaf senescence system that depends on SA biosynthesis and its core signaling receptor NPR1 in Arabidopsis thaliana. Here,using this system, we identified WRKY46 and WRKY6 as key components of the transcriptional machinery downstream of NPR1 signaling. Upon PBZ treatment, the wrky46 mutant exhibited significantly delayed leaf senescence. We demonstrate that NPR1 is essential for PBZ/SA-induced WRKY46 activation, whereas WRKY46 in turn enhances NPR1 expression. WRKY46 interacts with NPR1 in the nucleus, binding to the W-box of the WRKY6 promoter to induce its expression in response to SA signaling. Dysfunction of WRKY6 abolished PBZ-induced leaf senescence, while overexpression of WRKY6 was sufficient to accelerate leaf senescence even under normal growth conditions, suggesting that WRKY6 may serve as an integration node of multiple leaf senescence signaling pathways. Taken together,these findings reveal that the NPR1-WRKY46-WRKY6 signaling cascade plays a critical role in PBZ/SA-mediated leaf senescence in Arabidopsis.

关键词： leaf senescence NPR1 probenazole salicylic acid WRKY46 WRKY6

NON-YELLOWING2 （NYE2）, a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis

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Molecular Plant 2016年第4期9卷 624-627页

作者： Wu, Shouxin Li, Zhongpeng Yang, Lifeng Xie, Zuokun Chen, Junyi Zhang, Wei Liu, Tianqi Gao, Shan Gao, Jiong Zhu, Yihua Xin, Jiwen Ren, Guodong kuai, benke Fudan Univ Sch Life Sci Fudan Inst Plant Biol State Key Lab Genet Engn Shanghai 200438 Peoples R China Huazhong Agr Univ Coll Plant Sci & Technol Natl Key Lab Crop Genet Improvement Wuhan 430070 Peoples R China

Dear Editor,Plant senescence is an integral part of plant development, with old organs senescing in association with the emergence and development of nascent ones. Rapid chlorophyll （Chl） degradation, the most prominent event during green organ senescence, is proposed to be a key detoxification process to facilitate massive nutrient remobilization （Lim et al., 2007）.

关键词： senescence integral development

拟南芥叶片衰老前后叶绿素降解代谢基因表达和酶积累的模式及其影响因素分析

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复旦学报:自然科学版 2023年第5期62卷 565-574页

作者：窦锦慧陈俊毅鲁凡吴声栋蒯本科张鼎宇复旦大学遗传工程国家重点实验室上海200438 复旦大学生命科学学院上海200438

由叶绿素降解引起的叶片衰老褪绿不仅是植物衰老最直观的性状,还是植物进行营养动员的关键事件。近年来,进入衰老窗口(senescence window)后的叶片中叶绿素降解代谢酶基因(Chl catabolic genes,CCGs)的表达调控已经得到了较为充分的研究... 详细信息

由叶绿素降解引起的叶片衰老褪绿不仅是植物衰老最直观的性状,还是植物进行营养动员的关键事件。近年来,进入衰老窗口(senescence window)后的叶片中叶绿素降解代谢酶基因(Chl catabolic genes,CCGs)的表达调控已经得到了较为充分的研究,但在未衰老叶片中对CCGs表达模式的研究还鲜见报道。本研究中,我们分析了主要叶绿素降解代谢酶(Chl Catabolic Enzymes,CCEs)及其编码基因CCGs在自然衰老以及未衰老的拟南芥叶片中的表达模式。研究结果显示,在叶片自然衰老过程中,CCGs剧烈上调表达且CCEs蛋白逐渐累积,以催化衰老细胞中叶绿素的大量降解;值得注意的是,在长日照培养条件下,未衰老叶片中CCGs的表达存在显著的波动,其表达量在光照开启后开始上升,10:00~16:00之间达到峰值后逐渐下降。我们的分析显示,CCGs表达量的波动很有可能是光-暗交替导致的,与CCA1介导的节律信号没有明显的相关性。我们的研究结果提示,外界光信号对未衰老叶片中CCGs的表达调控起至关重要的作用。

关键词：绿色器官衰老叶绿素降解光信号叶绿素降解代谢酶叶绿素降解代谢酶基因

Cross-species identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence

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Cross-species identification of a novel chloroplast protein ...

不同大球盖菇菌株主要农艺性状和营养成分综合评价

2007中国植物生理学会全国学术会议

作者： Guodong Ren Kun An Yang Liao Xiao Zhou Yajun Cao benke kuai School of Life Sciences,Fudan University,220 Handan Rd,Shanghai 200433

＜正＞A dramatic increase of chlorophyll（Chl）degradation occurs during senescence of vegetative plant organs and fruit *** the biochemical pathway of Chl degradation has long been proposed,little is known about its regulatory *** of Chl degradation-disturbed mutants and subsequently isolation of responsible genes would greatly facilitate the elucidation of the regulation of Chl degradation. Here,we describe a non-yellowing mutant of Arabidopsis,nye1-1,in which 50% Chls was retained,

关键词： Chlorophyll degradation Leaf Senescence nyel-1 AtNYE1

来源： cnki会议评论

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食用菌学报 2022年第3期29卷 41-49页

作者：郝海波赵静杨慧张佳欣魏云辉蒯本科张津京陈辉上海市农业科学院食用菌研究所农业农村部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点实验室上海201403 复旦大学生命科学学院上海200438 上海光明森源生物科技有限公司上海201408 江西省农业科学院农业应用微生物研究所江西南昌330200

对13个大球盖菇(Stropharia rugosoannulata)菌株的农艺性状和营养成分进行综合评价。农艺性状分析结果表明:菌株DQ-1、DQ-4和DQ-5菌丝生长速度较快;菌株DQ-1和DQ-6出菇较早,单菇质量较大,平均单菇质量分别为(51.12±33.92)g和(51.23±3... 详细信息

对13个大球盖菇(Stropharia rugosoannulata)菌株的农艺性状和营养成分进行综合评价。农艺性状分析结果表明:菌株DQ-1、DQ-4和DQ-5菌丝生长速度较快;菌株DQ-1和DQ-6出菇较早,单菇质量较大,平均单菇质量分别为(51.12±33.92)g和(51.23±34.44)g;菌丝生长速度与出菇时间不存在相关性,而单菇质量与子实体长度、菌柄长度存在极显著相关性。子实体营养成分分析结果表明:菌株DQ-2、DQ-6和DQ-10总蛋白含量较高,菌株DQ-3、DQ-11和DQ-13总糖和多糖含量较高;菌株DQ-6粗脂肪含量最低,菌株DQ-1中粗纤维含量最高。各菌株子实体氨基酸含量为42.81~86.90 mg·g^(-1),其中菌株DQ-3、DQ-6和DQ-8必需氨基酸和呈味氨基酸含量较高。对大球盖菇必需氨基酸进行评分结果表明,甲硫氨酸+半胱氨酸为第一限制氨基酸,苏氨酸含量最高。综合评价结果表明:菌株DQ-6出菇最早、单菇质量较大、总蛋白和氨基酸含量较高,可进一步开展中试和示范栽培,而菌株DQ-3、DQ-8、DQ-10和DQ-13可作为选育高营养成分菌株的育种材料。

关键词：大球盖菇农艺性状营养成分相关性