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Symmetrically dispersed spectroscopic singlemolecule localization microscopy

Light(Science & Applications) 2020年第1期9卷 1154-1163页

作者： Ki-Hee Song Yang Zhang benjamin brenner Cheng Sun Hao F.Zhang Department of Biomedical Engineering Northwestern University2145 Sheridan Rd.EvanstonIL 60208USA Department of Mechanical Engineering Northwestern University2145

Spectroscopic single-molecule localization microscopy(sSMLM)was used to achieve simultaneous imaging and spectral analysis of single molecules for the first *** sSMLM fundamentally suffers from a reduced photon budget because the photons from individual stochastic emissions are divided into spatial and spectral ***,both spatial localization and spectral analysis only use a portion of the total photons,leading to reduced precisions in both *** improve the spatial and spectral precisions,we present symmetrically dispersed sSMLM,or SDsSMLM,to fully utilize all photons from individual stochastic emissions in both spatial and spectral *** achieved 10-nm spatial and 0.8-nm spectral precisions at a total photon budget of *** with the existing sSMLM using a 1:3 splitting ratio between spatial and spectral channels,SDsSMLM improved the spatial and spectral precisions by 42% and 10%,respectively,under the same photon *** also demonstrated multicolour imaging of fixed cells and three-dimensional single-particle tracking using *** enables more precise spectroscopic single-molecule analysis in broader cell biology and material science applications.

关键词： dispersed spectroscopic stochastic

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Boosting T cell immunity against cytomegalovirus:a potential strategy combating human aging and age-related diseases

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4417-4418页

作者： Matthias Wirth Walburgis brenner Oliver H.Kramer Department of Hematology Oncology and Cancer ImmunologyCampus Benjamin FranklinCharite-Universitatsmedizin Berlincorporate member of Freie Universitat Berlin and Humboldt-Universitat zu BerlinBerlinGermany University Medical Center Gottingen Department of GeneralVisceral and Pediatric SurgeryGottingenGermany German Cancer Consortium(DKTK) German Cancer Research Center(DKFZ)HeidelbergGermany Clinic for Obstetrics and Women's Health University Medical CenterJohannes Gutenberg University MainzLangenbeckstr.155131 MainzGermany Department of Toxicology University Medical CenterMainzGermany

In a recent publication in Cell,Hasegawa and colleagues reveal that cytotoxic CD4-positive cytotoxic T lymphocytes(CD4+CTLs)eliminate senescent fibroblasts with a surface expression of the immunologically important human leukocyte antigen class Il(HLA-)and the human cytomegalovirus glycoprotein B(HCMV-gB).1 This implies that a virome-immune cell interaction controls the fate of human skin cells that are linked to the aging process and associated diseases.

关键词： immunity cytomegalovirus cytotoxic

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