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Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous roots Extracts of Carica papaya L. and Cocos nucifera L.

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Advances in Microbiology 2023年第4期13卷 165-180页

作者： wendkouni leila.ma.ie esther belem-ka.ré Vincent Ouédraogo Bagora Bayala Alimata Bancé Estelle Ouédraogo Boubacar Yaro Lazare belemnaba Moussa Compaoré martin Kiendrébeogo Noufou Ouédraogo Department of Traditional Medicine and Pharmacopoeia-Pharmacy Health Sciences Research Institute Ouagadougou Burkina Faso Laboratory of Biochemistry and Applied Chemistry (LABIOCA) Doctoral School of Science and Technology Joseph KI-ZERBO University Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) Doctoral School of Science and Technology Joseph KI-ZERBO University Ouagadougou Burkina Faso International research Laboratory: Environnement Santé Sociétés (IRL 3189 ESS) CNRST/CNRS/UCAD/UGB/USTTB Ouagadougou Burkina Faso

Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC50 = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC50 = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.

关键词： Triterpenes and Sterols Content Antibacterial Biofilm Quorum Sensing Anti-Proliferative Medicinal Plants

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Anti-Inflammatory and Antioxidant Properties of the Aqueous Extracts of the Leaves of Opilia amentacea (Opiliaceae)

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Pharmacology & Pharmacy 2023年第9期14卷 329-347页

作者： rainatou Boly Abdoul Gilchrist Laurent Boly wendkouni leila.ma.ie esther belem-ka.ré kadiatou Tata Traoré Boukaré kaboré Ollo Youl Latifatou Sawadogo mathieu Nitièma Moumouni Koala Noufou Ouédraogo Estella Noëla Hoho Youl maminata Traoré-Coulibaly Laboratoire de recherche-Développement/Phytomédicaments et Médicaments (Lr-D/PM) Institut de Recherche en Sciences de la Santé (IRSS) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Ecole Doctorale Sciences et Technologie Laboratoire de Chimie Organique et Physique Appliquée (LCOPA) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Ecole Doctorale Sciences et Techniques Laboratoire de Biochimie et Chimie Appliquées (LABIOCA) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Ecole Doctorale Sciences de la Santé Laboratoire de Développement du Médicament (LADME) Centre de Formation de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM) Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Opilia amentacea (Opiliaceae) is a woody plant with multiple medicinal claimed effects. The present study aimed to assess the anti-inflammatory and antioxidant activities of the decoction and macerate extracts from the leaves of Opilia amentacea. Moreover, acute toxicity and phytochemical analysis were performed. The acute toxicity was evaluated on NMrI mice at 2000 mg/kg bw. The anti-inflammatory activity was studied using the carrageenan-induced mouse paw edema and the lipoxygenase inhibition assay. The radical scavenging (DPPH and ABTS), ferric-reducing antioxidant power (FrAP), and lipid peroxidation (LPO) assays were used to measure the antioxidant capacity of the extracts. Qualitative and quantitative methods served for identifying and quantifying the extract’s phytoconstituents. The decoction demonstrated low acute toxicity;the lethal dose was therefore estimated to be superior to 2000 mg/kg bw. The extracts significantly reduced the mouse paw’s thickness at 600 mg/kg bw. The extracts developed weak radical scavenging and lipid peroxidation inhibitory effects. However, the macerate showed a high ability (664.90 ± 0.71 mol Ascorbic Acid Equivalent/g dry extract) to reduce the ferric ions. Saponins, sterols, triterpenes, and flavonoids were qualitatively detected in the two extracts. Total phenolics (TP) and total flavonoids (TF) were found abundant in the extracts, especially the decoction (TP content (TPC) = 94.03 ± 2.66 mg GAE/g;TF content (TFC) = 35.05 ± 0.32 mg QE/g). Strong positive correlations existed between ferric-reducing capacity and TPC (r = 0.959) for the macerate, while TFC was mainly involved in the DPPH radical scavenging of the two extracts. Instead, most correlations were negative between the polyphenol compounds and the anti-inflammatory assays. The results indicate potent in vivo anti-inflammatory and in vitro antioxidant effects of the aqueous extracts from the leaves of Opilia amentacea. Further studies are needed to find the anti-inflamm

关键词： Opilia amentacea Aqueous Extracts Acute Toxicity Anti-Inflammatory Antioxidant

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Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on the Isolated Uterus of Mice

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Pharmacology & Pharmacy 2023年第8期14卷 252-270页

作者： belem-ka.ré wendkouni leila.ma.ie esther Nitiéma mathieu Odjo Blondine Saïdath maman Noura Oumarou Yaro Boubacar kaboré Boukaré Somda Gaëtan Donzéo Traoré Tata kadiatou Koala Moumouni Ilboudo Sylvain Ouédraogo Moussa ka.ré Elie Ouédraogo Noufou Laboratoire de recherche-Développement de Phytomédicaments et Médicaments (Lr-D/PM) Institut de Recherche en Sciences de la Santé (IRSS/CNRST) Ouagadougou Burkina Faso Laboratoire du Développement du Médicament (LADME)/Centre de Formation de Recherche et d’Expertises en Sciences du Médicament (CEA-CFOREM) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Laboratoire de Chimie Organique et de Physique Appliquée (LCOPA) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Agence Nationale pour la Sécurité Sanitaire de l’Environnement de l’Alimentation du Travail et des Produits de Santé (ANSSEAT) Ouagadougou Burkina Faso

Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FrAP, and LPO). results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A2 was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation. No significant difference was noted in the phospholipase inhibition between the extracts and betamethasone. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC50 value of 55.82 ± 1.15 μg/mL. The lethal dose (LD50) of extracts was estimated to be superior to 2000 mg/kg body weight (bw). The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC50) was 5.33 × 10−8 ± 0.32 mg/mL for oxytocin, 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate childbirth.

关键词： Anastatica hierochuntica Phytoconstituents Antioxidant Anti-Inflammatory Uterine Contractility

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幽门螺杆菌治疗后长期使用质子泵抑制剂与胃癌发生的风险:以人群为基础的研究

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英国医学杂志中文版 2019年第7期22卷 422-422页

作者： ka Shing Cheung esther W Chan Angel Y S Wong Lijia Chen lan C K Wong Wai Keung Leung 彭浩(译) 不详应急总医院普外肿瘤科

目的质子泵抑制剂(PPIs)与胃萎缩恶化相关,尤其是在幽门螺杆菌(HP)感染者中。我们确定了在接受抗HP治疗的HP感染者中,PPIs使用与胃癌(GC)之间的相关性。设计本研究以中国香港全区健康资料库为基础。我们首先确认出自2003至2012年间接受... 详细信息

目的质子泵抑制剂(PPIs)与胃萎缩恶化相关,尤其是在幽门螺杆菌(HP)感染者中。我们确定了在接受抗HP治疗的HP感染者中,PPIs使用与胃癌(GC)之间的相关性。设计本研究以中国香港全区健康资料库为基础。我们首先确认出自2003至2012年间接受过以克拉霉素为基础的三联疗法门诊处方的成年人。排除标准:使用该处方治疗失败的患者,HP治疗后12个月内确诊为GC的患者,以及治疗后发生胃溃疡者。GC排除前的6个月内开始PPIs或H2受体阻滞剂(H2rA)治疗,以避免原始偏倚。通过C。x比例风险模型的倾向分数调整,评估GC发生风险与质子泵抑制剂二者之间的关系。H2rA作为阴性对照暴露。结果共63 397名合格受试者入选,在平均7.6年的随访时间中,有153(0.24%)名发生GC。PPIs使用与GC发生风险增高相关[危害比(Hr)2.44,95%可信区间(C/)1.42-4.20],而H2rA无相关性(“r 0.72,95%CI 0.48-1.07)0GC发生的风险随PPIs使用时间的延长而增高(PPIs使用时间/年22年和年的GC发生风险分别为:Hr 5.04.95%CI 1.23-20.61;6.65,1.62-27.26和8.34,95%CI 2.02-34.41)。PPIs使用相比非PPIs使用,调整的绝对风险差为每10000人年超过4.29 GC(95%C/1.25-9.54)。结论即使在HP根除治疗后,长期使用质子泵抑制剂仍与GC的发生风险增加相关。

关键词： Cox比例风险模型质子泵抑制剂治疗失败幽门螺杆菌胃癌发生 H2受体阻滞剂人群 HP根除治疗

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