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中国人兽共患病杂志 2005年第3期21卷 193-196页

作者：高琪周华云 Cooper R.D 李凤华苏云普朱国鼎曹俊 beebe n.w 江苏省寄生虫病防治研究所无锡214064 Australia Army Malaria Institute 辽宁省疾病预防控制中心河南省疾病预防控制中心 Institute for the Biotechnology of Infectious Disease University of TechnologySydneyAustralia

目的　建立一种能鉴别赫坎按蚊种团内不同近缘种按蚊的基因鉴别技术。方法　依据赫坎按蚊种团内中华按蚊、嗜人按蚊、雷氏按蚊和八代按蚊 4种近缘种按蚊ITS2 区段的基因序列特征设计特异性引物 ,建立一种能鉴别赫坎按蚊种团内中华按蚊... 详细信息

目的　建立一种能鉴别赫坎按蚊种团内不同近缘种按蚊的基因鉴别技术。方法　依据赫坎按蚊种团内中华按蚊、嗜人按蚊、雷氏按蚊和八代按蚊 4种近缘种按蚊ITS2 区段的基因序列特征设计特异性引物 ,建立一种能鉴别赫坎按蚊种团内中华按蚊、嗜人按蚊、雷氏按蚊和八代按蚊 4种近缘种按蚊的简便的PCR基因鉴别技术 ,并采用传统的形态学分类方法和新建立的基因鉴别技术对从辽宁、河南省以及在朝鲜现场捕获的按蚊分别进行了形态学和基因鉴别及比较。结果与结论　新建立的基因鉴别技术能准确鉴别赫坎按蚊种团内中华按蚊、嗜人按蚊、雷氏按蚊和八代按蚊 4种近缘种按蚊。具有比传统的形态学分类方法准确 ,比已有的PCR-RFLP蚊种基因鉴别技术更简便、价廉和省时的特点。

关键词：嗜人按蚊中华按蚊八代按蚊雷氏按蚊核糖体核酸内转录间隔2 聚合酶链反应

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Microphysiological systems for solid tumor immunotherapy:opportunities and challenges

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Microsystems & nanoengineering 2023年第6期9卷 77-106页

作者： Sara Abizanda-Campo María Virumbrales-Muñoz Mouhita Humayun Ines Marmol David J.beebe Ignacio Ochoa Sara Oliván Jose M.Ayuso Department of Dermatology University of Wisconsin-MadisonMadisonWIUSA University of wisconsin Carbone Cancer Center MadisonWIUSA Department of Biomedical Engineering University of WisconsinMadisonWIUSA Tissue Microenvironment Lab(TME lab) Aragón Institute of Engineering Research(I3A)University of ZaragozaZaragozaSpain Instituto de Investigación Sanitaria Aragón(IISA) ZaragozaSpain Centro Investigación Biomédica en Red.Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)ZaragozaSpain Department of Obstetrics and Gynecology University of Wisconsin-MadisonMadisonWIUSA Department of Biological Engineering Massachusetts Institute of Technology CambridgeCambridgeMAUSA Department of Pathology&Laboratory Medicine University of WisconsinMadisonWIUSA

Immunotherapy remains more effective for hematologic tumors than for solid *** of the main challenges to immunotherapy of solid tumors is the immunosuppressive microenvironment these tumors generate,which limits the cytotoxic capabilities of immune effector cells(e.g.,cytotoxic T and natural killer cells).This microenvironment is characterized by hypoxia,nutrient starvation,accumulated waste products,and acidic ***-hijacked cells,such as fibroblasts,macrophages,and T regulatory cells,also contribute to this inhospitable microenvironment for immune cells by secreting immunosuppressive cytokines that suppress the antitumor immune response and lead to immune ***,there is a strong interest in developing n.w drugs and cell formulations that modulate the tumor microenvironment and reduce tumor cell immune *** systems(MPSs)are versatile tools that may accelerate the development and evaluation of these therapies,although specific examples showcasing the potential of MPSs remain *** in microtechnologies have led to the development of sophisticated microfluidic devices used to recapitulate tumor *** resulting models,also kn.wn as microphysiological systems(MPSs),are versatile tools with which to decipher the molecular mechanisms driving immune cell antitumor cytotoxicity,immune cell exhaustion,and immune cell exclusion and to evaluate n.w targeted ***,we review existing microphysiological platforms to study immuno-oncological applications and discuss challenges and opportunities in the field.

关键词： immunotherapy versatile solid

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玻璃体切割术增加晶状体高氧中暴露:一种核性白内障形成的可能机制

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世界核心医学期刊文摘（眼科学分册） 2005年第7期1卷 11-12页

作者： Holekamp n.M. beebe D.C. 司冰心 Holekamp Barnes Retina Institute 1600 South Brentwood Boulevard St. Louis MO 63144 United States

Purpose: To report vitreous oxygen tension before, immediately after, and at longer times after vitrectomy. Design: A prospective, interventional consecutive case series. Methods: Oxygen.was measured using an optical oxygen sensor in patients undergoing vitrectomy. Intraoperatively, oxygen measurements were taken before and after vitrectomy in two intraocular locations: adjacent to the lens and in the mid- vitreous. Results: Sixty- nine eyes underwent oxygen tension measurements at the time of vitrectomy. In baseline eyes, oxygen tension in the vitreous was low, measuring 8.7± 0.6 mm Hg adjacent to the lens and 7.1± 0.5 mm Hg in the mid- vitreous. The difference between the two locations was statistically significant (Pndicating that vitreous gel maintains an intraocular oxygen gradient. Immediately after vitrectomy, oxygen tension in the fluid- filled eye was higher, measuring 69.6± 4.8 mm Hg adjacent to the lens and 75.6± 4.1 mm Hg in the mid- vitreous. There was no statistically significant oxygen gradient between the two locations. The difference in oxygen tension pre- and postvitrectomy is highly statistically signifi- cant (P ns, P n eyes with a history of vitrectomy and previous removal of the vitreous gel, the intraocular oxygen tension.was significantly higher than in eyes with a formed vitreous gel undergoing a first vitrectomy (Pns, Pnclusion: Vitrectomy surgery significantly increases intraocular oxygen tension during and for prolonged periods after surgery. This exposes the crystalline lens to abnormally high oxygen and may lead to nuclear cataract formation.

关键词：玻璃体切割术核性白内障高氧氧张力玻璃体手术干预性手术期间氧传感器于术测量数值

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