检索结果-南通市图书馆

Investigation of HCAR2 antagonists as a potential strategy to modulate bovine leukocytes

journal of Animal Science and Biotechnology 2024年第4期15卷 1567-1577页

作者： Laman K.Mamedova Kirby C.Krogstad Paiton O.McDonald Laxman Pokhrel Duy H.Hua Evan C.Titgemeyer barry j.bradford Department of Animal Science Michigan State UniversityEast LansingMichigan 48824USA Department of Animal Sciences and Industry Kansas State UniversityManhattanKS 66506USA Comparative Medicine and Integrative Biology East LansingMI 48824USA Department of Chemistry Kansas State UniversityManhattanKS 66506USA

Background Dairy cows experiencing ketosis after calving suffer greater disease incidence and are at greater risk of leaving the herd. In vitro administration of beta-hydroxybutyric acid(BHBA;the primary blood ketone) has inhibitory effects on the function of bovine leukocytes. BHBA is a ligand of HCAR2 and the activation of these receptors promotes an anti-inflammatory response which may be related with immunosuppression observed in transition dairy cattle. The objective of this study was to identify and test antagonists for HCAR2 in bovine immune cells cultured with *** We observed expression of HCAR2 at the protein level within lymphocytes, monocytes, and granulocytes. The proportion of cells expressing HCAR2 tended to be greater in mid-lactation compared to early lactation cows;the increase was a result of increased proportion of T and B cells expressing HCAR2. Stimulation of HCAR2 with niacin or BHBA promoted Ca^(2+) mobilization in neutrophils and mononuclear cells. Mononuclear cells treated with BHBA had diminished intracellular Ca^(2+) responses when HCAR2 was knocked down by si RNA silencing, indicating Ca^(2+) mobilization was mediated by HCAR2 signaling. Two candidate antagonists for HCAR2, synthesized from niacin(NA-1 and NA-5), were tested;monocytes and neutrophils pre-treated with NA-1 and NA-5 had reduced Ca^(2+) mobilization after incubation with BHBA. Furthermore, NA-5 but not NA-1 prevented BHBA-associated reductions in cyclic *** We demonstrated that HCAR2 is present on bovine leukocytes and has greater expression later in lactation. We confirmed that BHBA and niacin derived HCAR2 antagonists alter bovine leukocyte activity. Our results demonstrate that both BHBA and niacin affect bovine leukocyte Ca^(2+) mobilization in a HCAR2-dependent manner.

关键词： Flow cytometry GPR109A HM74A Ketosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Postpartum meloxicam administration alters plasma haptoglobin, polyunsaturated fatty acid, and oxylipid concentrations in postpartum ewes

引用

journal of Animal Science and Biotechnology 2020年第4期11卷 1047-1060页

作者： Katie EOlagaray barry j.bradford Lorraine M.Sordillo jeffery C.Gandy Laman K.Mamedova Turner H.Swartz Trey D.jackson Emma K.Persoon Caitlin S.Shugart Curtis R.Youngs Department of Animal Sciences and Industry Kansas State UniversityManhattan 66506USA College of Veterinary Medicine Michigan State University2265K Anthony HallEast LansingMI 48824-1225USA Department of Animal Science Iowa State UniversityAmesIAUSA.

Background: Postpartum inflammation is a natural and necessary response;however, a dysfunctional inflammatory response can be detrimental to animal productivity. The objective of this study was to determine the effects of a non-steroidal anti-inflammatory drug(meloxicam) on ewe postpartum inflammatory response, ewe plasma polyunsaturated fatty acid and oxylipid concentrations, and lamb ***: After lambing, 36 Hampshire and Hampshire × Suffolk ewes were sequentially assigned within type of birth to control(n = 17) or meloxicam orally administered on d 1 and 4 of lactation(MEL;90 mg, n = 19). Milk and blood samples were collected on d 1(prior to treatment) and d 4. Milk glucose-6-phosphate was not affected by MEL. Plasma haptoglobin(Hp) concentrations were less for MEL ewes;control ewes with greater d 1 Hp concentrations had elevated Hp on d 4, but this was not the case for MEL-treated ewes. Treatment with MEL increased plasma arachidonic acid concentration by more than 4-fold in ewes rearing singles but decreased concentrations of 9,10-dihydroxyoctadecenoic acid, prostaglandin F2α, 8-iso-prostaglandin E2, and 8,9-dihydroxyeicosatetraenoic acid. Nine oxylipids in plasma had interactions of treatment with d 1 Hp concentration,all of which revealed positive associations between d 1 Hp and d 4 oxylipid concentrations for CON, but neutral or negative relationships for MEL. MEL decreased 13-hydroxyoctadecadienoic acid:13-oxooctadecadienoic acid ratio and tended to increase 9-hydroxyoctadecadienoic acid:9-oxooctadecadienoic acid ratio(both dependent on d 1 values), indicating progressive metabolism of linoleic acid-derived oxylipids occurred by enzymatic oxidation after MEL treatment. Meloxicam reduced oxylipids generated across oxygenation pathways, potentially due to an improved redox ***: Postpartum MEL treatment of ewes decreased plasma concentrations of Hp and several oxylipids,with the greatest impact in ewes with biomarkers reflecting a greate

关键词： Eicosanoid Inflammation Lactation Nonsteroidal anti-inflammatory drug Sheep

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Restricted nutrient intake does not alter serum-mediated measures of implant response in cell culture

引用

journal of Animal Science and Biotechnology 2014年第1期5卷 80-87页

作者： Christopher D Reinhardt Tiffany L Lee Daniel U Thomson Laman K Mamedova barry j.bradford Department of Animal Sciences and Industry 232 Weber Hall Kansas State University Department of Clinical Sciences A-111 Mosier Hall Kansas State University

Background： During nutritional stress, reduced intake may reduce the efficacy of anabolic implants. This study was conducted to evaluate basic cellular responses to a growth promotant implant at two intake levels. Methods： Sixteen crossbred steers （293 ± 19.3 kg） were used to evaluate the impact of anabolic implants in either an adequate or a restricted nutritional state. Steers were trained to individual Calan gates, and then randomly assigned to 1 of 4 treatments in a 2 × 2 factorial arrangement. Treatments consisted off presence or absence of an anabolic growth implant （Revalor-XS, 200 mg TBA and 40 mg estradiol; IMPLANT or CONTROL） and a moderate energy, pelleted, starting cattle diet fed at either 2.0 × or 1.0 × maintenance energy （NEM） requirements （HIGH or LOW）. Serum （d O, 14, and 28） was used for application to bovine muscle satellite cells. After treatment with the serum （20% of total media） from the trial cattle, the satellite cells were incubated for 72 h. Protein abundance of myosin heavy chain （MHC）, phosphorylated extracellular signal-related kinase （phospho-ERK）, and phosphorylated mammalian target of rapamycin （phospho-mTOR） were analyzed to determine the effects of implant, intake, and their interaction （applied via the serum）. Results： Intake had no effect on MHC （P = 0.85） but IMPLANT increased （P 〈 0.01） MHC abundance vs. CONTROL. Implant status, intake status, and the interaction had no effect on the abundance of phospho-ERK （P〉0.23）. Implanting increased phospho-mTOR （P 〈 0.01） but there was no effect （P 〉 0.51） of intake or intake x implant. Conclusions： The nearly complete lack of interaction between implant and nutritional status indicates that the signaling molecules measured herein respond to implants and nutritional status independently. Furthermore, results suggest that the muscle hypertrophic effects of anabolic implants may not be mediated by circulating IGF-1.

关键词： Beef cattle Implant Muscle Myosin heavy chain Nutrient restriction Satellite cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

用改进逆境萌发的滲透引发来熟练掌握种子水分关系

引用

种子 1987年第6期 68-74页

作者： Kent j.bradford ,陈暖

对许多一年生作物来讲,从种植到定植这段时间,是生产的关键时期。直播作物的出苗和齐苗,对最终产量和质量有很大影响,特别是蔬菜。为获得理想植物群体,精耕细作保证种植的粒粒种子都能定植。此外,价格昂贵的

关键词：种子含水量发根等式 MPa 膨压渗透调节种子含水率紧张压相对紧涨度 RWC PEG 种子引发逆境

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

被忽视的经济增长机遇——中美商业服务贸易

引用

新金融评论 2013年第3期 136-155页

作者： j.bradford jensen 本刊编辑乔治敦大学商学院

当前中美之间有一项重要的商贸机会——商业服务贸易一直被忽视。美国已经连续多年存在大量贸易逆差,亟需扩大出口,尤其是对那些经济增长较快的发展中国家的出口。对中国来说,提高制造业的价值链就需要获得工程、设计、开发、检验、市... 详细信息

当前中美之间有一项重要的商贸机会——商业服务贸易一直被忽视。美国已经连续多年存在大量贸易逆差,亟需扩大出口,尤其是对那些经济增长较快的发展中国家的出口。对中国来说,提高制造业的价值链就需要获得工程、设计、开发、检验、市场营销、广告推广、物流和配送等多方面的高效尖端服务。目前中国的商业服务业规模还比较小,收费也比较高。虽然长期来看,教育可以解决这个问题,但短期来看,进口服务是一个合适的选择。很多服务都是可交易的,但中国对服务业贸易设置的壁垒还是较多。中美之间的商业服务贸易可开发的潜力很大,这也将给两个国家带来好处。当然,抓住这个机遇就必须要减少政策障碍。

关键词：商业服务服务业贸易政策障碍

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Global climate change is confounding species conservation strategies

引用

Integrative Zoology 2012年第2期7卷 158-164页

作者： Harold KOOPOWITZ bradford A.HAWKINS Department of Ecology and Evolutionary Biology University of CaliforniaIrvineCaliforniaUSA

Most organisms face similar problems with respect to their conservation in the face of global climate ***,we examine probable effects of climate change on the hyperdiverse plant family *** the 20th century,the major concerns for orchid conservation revolved around unsustainable harvest for the orchid trade and,more importantly,land conversion from natural ecosystems to those unable to support wild orchid pop*** conversion included logging,fire regimes and forest conversions to agricultural ***though those forms of degradation continue,an additional suite of threats has emerged,fueled by global climate *** climate change involves more than responses of orchid populations to increases in ambient *** temperature induces secondary effects that can be more significant than simple changes in *** these new threats are extended and prolonged fire seasons,rising sea levels,increases in cyclonic storms,seasonal climate shifts,changes in orthographic wind dew point and increased *** long-term outlook for orchid biodiversity in the wild is dismal,as it is for many animal groups,and we need to start rethinking strategies for conservation in a rapidly changing world.

关键词： biodiversity climate change conservation biology ex situ conservation orchids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

论美中双边投资协定的重要性:潜在经济效益

引用

新金融评论 2017年第3期 97-112页

作者： Lee Branstetter Britta Glennon j.bradford jensen 卡内基梅隆大学乔治城大学

虽然中国在经济开放方面已经取得了重大进展,包括允许私营企业、国际贸易和外商投资等进入市场,但这一进程还远未结束。外商直接投资中国服务性行业的壁垒相对较高,从而降低了服务行业生产力,抬高了价格,限制了中国消费者和企业的选择,... 详细信息

虽然中国在经济开放方面已经取得了重大进展,包括允许私营企业、国际贸易和外商投资等进入市场,但这一进程还远未结束。外商直接投资中国服务性行业的壁垒相对较高,从而降低了服务行业生产力,抬高了价格,限制了中国消费者和企业的选择,最终抑制了经济增长。美中双边投资协定(BIT)则能在消除外商直接投资壁垒、促进两国经济发展上发挥重要作用。一个高标准的BIT将对深化开放外资以及提高中国服务性行业的竞争与发展水平产生重大影响。加大外商直接投资或将成为缩小中国第三产业和国际先进标准间生产力鸿沟的契机。若放开本土竞争与实施BIT同时进行,那么一个高标准的BIT将在极大程度上提升中国经济增长潜力。尽管评估BIT的影响较为困难,我们参考了一系列最新研究报告估算潜在收益范围。我们的研究表明签订BIT可能带来巨大收益。

关键词：美中双边投资协定服务业外商直接投资

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Robustness Assessment and Adaptive FDI for Car Engine

引用

International journal of Automation and computing 2008年第2期5卷 109-118页

作者： Mahavir Singh Sangha j.barry Gomm Control Systems Research Group School of EngineeringLiverpool John Moores University

A new on-line fault detection and isolation （FDI） scheme proposed for engines using an adaptive neural network classifier is evaluated for a wide range of operational modes to check the robustness of the scheme in this paper. The neural classifier is adaptive to cope with the significant parameter uncertainty, disturbances, and environment changes. The developed scheme is capable of diagnosing faults in on-line mode and the FDI for the closed-loop system with can be directly implemented in an on-board crankshaft speed feedback is investigated by diagnosis system （hardware）. The robustness of testing it for a wide range of operational modes including robustness against fixed and sinusoidal throttle angle inputs, change in load, change in an engine parameter, and all these changes occurring at the same time. The evaluations are performed using a mean value engine model （MVEM）, which is a widely used benchmark model for engine control system and FDI system design. The simulation results confirm the robustness of the proposed method for various uncertainties and disturbances.

关键词： On-board fault diagnosis automotive engines adaptive neural networks (ANNs) fault classification robustness assessment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

鹦鹉经济学 002

引用

21世纪经济报道

作者： j.bradford DeLong 刘波

据说19世纪早期的英国经济学家麦克库洛赫说过，只要教会一个鹦鹉说“供应和需求”，它就会成为一名合格的政治经济学家。事实上，如果经济学家真的是这样就好了，其实很多经济学家还没有那鹦鹉聪明。　　举个例子，现在美国很多人在...

据说19世纪早期的英国经济学家麦克库洛赫说过，只要教会一个鹦鹉说“供应和需求”，它就会成为一名合格的政治经济学家。事实上，如果经济学家真的是这样就好了，其实很多经济学家还没有那鹦鹉聪明。　　举个例子，现在美国很多人在说，政府采取的缓解失业的措施不会奏效?

关键词：

来源： cnki报纸评论

在线全文

cnki报纸

学校读者我要写书评

暂无评论

Multitarget stool DNA for colorectal cancer screening:A review and commentary on the United States Preventive Services Draft Guidelines

引用

World journal of Gastrointestinal Oncology 2016年第5期8卷 450-458页

作者： barry M Berger Bernard Levin Robert j Hilsden Medical Affairs Exact Sciences Corporation Scientific Advisory Board Exact Sciences Departments of Medicine and Community Health Sciences University of Calgary

Multitarget stool DNA(mt-sDNA) testing was approved for average risk colorectal cancer(CRC) screening by the United States Food and Drug Administration and thereafter reimbursed for use by the Medicare program(2014).The United States Preventive Services Task Force(USPSTF) October 2015 draft recommendation for CRC screening included mt-s DNA as an "alternative" screening test that "may be useful in select clinical circumstances",despite its very high sensitivity for early stage *** evidence supporting mt-s DNA for routine screening use is *** clinical efficacy of mt-s DNA as measured by sensitivity,specificity,life-years gained(LYG),and CRC deaths averted is similar to or exceeds that of the other more specifically recommended screening options included in the draft document,especially those requiring annual testing *** a population with primarily irregular screening participation,tests with the highest point sensitivity and reasonable specificity are more likely to favorably impact CRC related morbidity and mortality than those depending on annual *** paper reviews the evidence supporting mt-s DNA for routine screening and demonstrates,using USPSTF's modeling data,that mt-s DNA at three-year intervals provides significant clinical net benefits and fewer complications per LYG than annual fecal immunochemical testing,high sensitivity guaiac based fecal occult blood testing and 10-year colonoscopy screening.

关键词： Colorectal cancer screening Multitarget stool DNA Stool DNA The United States Preventive Services Task Force Cancer Intervention Surveillance Modeling Network Fecal immunological technique Modeling Interval

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：