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Cryo-EM structure of a tetrameric photosystem I from Chroococcidiopsis Ts-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium

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Plant Communications 2022年第1期3卷 53-70页

作者： Dmitry A.semchonok jyotirmoy Mondal Connor j.Cooper Katrina schlum Meng Li Muhamed Amin Carlos O.s.sorzano Erney Ramirez-Aportela Panagiotis L.Kastritis Egbert j.Boekema Albert Guskov barry D.Bruce Groningen Biomolecular sciences&Biotechnology Institute University of GroningenGroningenthe Netherlands Biochemistry&Cellular and Molecular Biology Department University of TennesseeKnoxvilleTNUSA Program in Genome science and Technology University of TennesseeKnoxvilleTNUSA Bredesen Center for Interdisciplinary Research&Education University of TennesseeKnoxvilleTNUSA Department of sciences University College GroningenGroningenthe Netherlands Biocomputing Unit National Center for Biotechnology(CSIC)Darwin 3Campus Universidad Auto´noma de MadridCantoblanco28049 MadridSpain Universidad CEU san Pablo Campus Urb.Monteprı´ncipeBoadilla del Monte28668 MadridSpain Institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany Microbiology Department University of TennesseeKnoxvilleTNUSA Present address:Institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany school of Oceanography University of WashingtonSeattleWAUSA

Photosystem I(PsI)is one of two photosystems involved in oxygenic *** of cyanobacteria exists in monomeric,trimeric,and tetrameric forms,in contrast to the strictly monomeric form of PsI in plants and *** tetrameric organization raises questions about its structural,physiological,and evolutionary *** we report the3.72 A˚resolution cryo-electron microscopy structure of tetrameric PsI from the thermophilic,unicellular cyanobacterium Chroococcidiopsis *** structure resolves 44 subunits and 448 cofactor *** conclude that the tetramer is arranged via two different interfaces resulting from a dimer-of-dimers *** localization of chlorophyll molecules permits an excitation energy pathway within and between adjacent *** analysis reveals conserved regions in the PsaL subunit that correlate with the oligomeric *** PsI may function as a key evolutionary step between the trimeric and monomeric forms of PsI organization in photosynthetic organisms.

关键词： cryo-EM photosystem I non-heterocyst-forming cyanobacteria Chroococcidiopsis evolution of chloroplast high light adaptation

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Abdominal ultrasonography of the pediatric gastrointestinal tract

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World journal of Radiology 2016年第7期8卷 656-667页

作者： Heather I gale Michael s Gee sjirk j Westra Katherine Nimkin Department of Radiology Division of Pediatric Radio-logy Massachusetts General Hospital

Ultrasound is an invaluable imaging modality in the evaluation of pediatric gastrointestinal pathology; it can provide real-time evaluation of the bowel without the need for sedation or intravenous contrast. Recent improvements in ultrasound technique can be utilized to improve detection of bowel pathology in children: Higher resolution probes, color Doppler, harmonic and panoramic imaging are excellent tools in this setting. Graded compression and cine clips provide dynamic information and oral and intravenous contrast agents aid in detection of bowel wall pathology. Ultrasound of the bowel in children is typically a targeted exam; common indications include evaluation for appendicitis, pyloric stenosis and intussusception. Bowel abnormalities that are detected prenatally can be evaluated after birth with ultrasound. Likewise, acquired conditions such as bowel hematoma, bowel infections and hernias can be detected with ultrasound. Rare bowel neoplasms, vascular disorders and foreign bodies may first be detected with sonography, as well. At some centers, comprehensive exams of the gastrointestinal tract are performed on children with inflammatory bowel disease and celiac disease to evaluate for disease activity or to confirm the diagnosis. The goal of this article is to review up-to-date imaging techniques, normal sonographic anatomy, and characteristic sonographic features of common and uncommon disorders affecting the gastrointestinal tract in children.

关键词： Ultrasound Pediatric Gastrointestinal tract Bowel Enteritis

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Late Mesozoic and Cenozoic thermotectonic evolution along a transect from the north China craton through the Qinling orogen into the Yangtze craton,central China

Late Mesozoic and Cenozoic thermotectonic evolution along a ...

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中国科学院地质与地球物理研究所2006学术年会

作者： Asaf Raza Kyoungwon Min barry P.Kohn Peter W.Reiners Richard A.Ketcham Andrew j.W.Gleadow school of Earth sciences University of MelbourneMelbourneAustralia Department of Geology and Geophysics Yale UniversityNew HavenConnecticutUSA Department of Geological sciences University of Texas at AustinAustinTexasUSA

Cretaceous and Cenozoic reactivation of the Triassic Qinling-Dabie orogen between the north China and Yangtze cratons resulted from the combined effects of Pacific subduction-back-arc extension in east China and collisions in west *** report new apatite fission track and apatite and zircon(U-Th)/He data from east Qinling along a >400-km-long N-s transect from Huashan through the Qinling orogen to *** ages show a general pattern of younging *** major cooling phases are defined by modeling the multiple thermochronologic data *** first phase occurred locally in the North and south Qinling during the late Triassic to early jurassic,following heating associated with the Triassic Yangtze subduction and exhumation of the Wudang metamorphic core complex on the cratonal edge.A second phase represents regional exhumation between 100 and 60 Ma,coeval with rifting marked by the Late Cretaceous-Eocene(K2-E) red bed deposition in eastern China and possibly indicating a link with Pacific subduction-back-arc extension in eastern China;however,it may also have been superimposed by eastward tectonic escape resulting from the Lhasa-West Burma-Qiangtang-Indochina *** third cooling phase was initiated at 5 Ma exclusively in the north Qinling and in the footwall of the graben-bounding normal fault of the Weihe graben in the Lesser *** suggest the third phase was related to reactivation of the Qinling fault system as a result of eastward tectonic escape imposed by the India-Asia collision at 0 Ma.

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颅缝早闭症相关实验荧光定量PCR内参基因的选择研究

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组织工程与重建外科杂志 2012年第3期8卷 140-145页

作者：杨娴娴 jodie T Hatfield susan j Hinze 穆雄铮 Peter j Anderson barry C Powell 上海交通大学医学院附属第九人民医院整复外科上海市200011 澳大利亚颅颌面外科中心阿德莱德妇女儿童健康研究中心复旦大学医学院附属华山医院整形外科上海市200040

目的探讨用于颅缝早闭症相关实验的荧光定量PCR的最佳内参基因。方法应用与颅骨成骨、颅缝闭合相关的3组实验标本,分别为:Fgfr2cC342Y/+Crouzon综合征小鼠颅缝组织、颅缝早闭患者体外培养颅缝原代细胞和体外培养的小鼠Kusa 4b 10成骨细... 详细信息

目的探讨用于颅缝早闭症相关实验的荧光定量PCR的最佳内参基因。方法应用与颅骨成骨、颅缝闭合相关的3组实验标本,分别为:Fgfr2cC342Y/+Crouzon综合征小鼠颅缝组织、颅缝早闭患者体外培养颅缝原代细胞和体外培养的小鼠Kusa 4b 10成骨细胞株。以常用的候选管家基因作为研究对象,利用geNorm软件对RT-qPCR结果进行比较分析,以筛选出在不同组织细胞中表达最为稳定的管家基因作为内参。结果Fgfr2cC342Y/+小鼠颅缝组织,Cyc1-Gapdh-Canx为最佳组合;颅缝早闭症患者体外培养颅缝原代细胞,18s rRNA和ATP5B为最佳组合;体外培养的小鼠Kusa 4b10成骨细胞株,18s rRNA和Canx为最佳组合。选择不同内参基因对目的基因RT-qPCR的结果影响显著。结论在设计RT-qPCR实验之前,应针对所选择的标本类型与种类进行管家基因稳定性分析,以增加结果的可信度。

关键词：颅缝早闭症荧光定量聚合酶链反应geNorm分析

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塞拉利昂太古宙基底中不一致的Rb—sr和Pb—Pb全岩等时年龄的意义

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华北地质 1982年第1期 67-76页

作者： R.D.Beckinsale N.H.gale R.j.Pankhurst A.Macfarlane M.j.Crow j.W.Arthurs A.F.Wilkinson 李顺智英国地质科学研究所地球化学局英国牛津大学地质矿产系英国剑桥大学南极观察站英国地质科学研究所对外局英国地质科学研究所对外局英国地质科学研究所对外局英国地质科学研究所对外局

英国地质科学研究所一个研究队（I.G.s）,通过对塞拉利昂北部的野外制图和构造研究,建立了西非克拉通太古宙地层层序,划分出一个较老的利欧尼亚（Leonian）花岗岩—绿岩区,它在利比里亚构造—变质事件结束时形成的,一个花岗岩—绿岩区... 详细信息

英国地质科学研究所一个研究队（I.G.s）,通过对塞拉利昂北部的野外制图和构造研究,建立了西非克拉通太古宙地层层序,划分出一个较老的利欧尼亚（Leonian）花岗岩—绿岩区,它在利比里亚构造—变质事件结束时形成的,一个花岗岩—绿岩区之前就经受过一次构造—变质事件。在法杜古区,在具有利欧尼亚构造的花岗片麻岩中,得到在统计学上是合理的但不一致的Pb—Pb和Rb—sr全岩等时年龄分别为2959±50百万年和2753±61百万年（误差2σ）。这些年龄是和塞拉利昂其他地区的利欧尼亚和利比里亚构造放射年龄可对比,可以认为,法杜古区的Rb—sr全岩等时年龄已受到利比里亚事件的影响。法杜古片麻岩的Pb—Pb全岩等时年龄和一个先前测定的（经重新计算并作部分检查）取自塞拉利昂东南花岗片麻岩中的Rb—sr全岩等时年龄为2980±80百万年,为利欧尼亚构造—变质事件提供了一个确切的年龄,约2970百万年。利欧尼亚花岗岩类的。87sr/86sr初始值和现代单阶段模式U238/Pb204值很难与地幔的值相区分,但并不排除这种可能性,即这些花岗岩类是来自地壳或下部地壳只经历了一个短暂时期的那些母质中。法杜古区花岗片麻岩的2753±61百万年的Rb—sr全岩等时年龄为塞拉利昂北部的利比里亚事件提供了一个确切的年龄。在野外划分出比利欧尼亚更老的（即老于2970百万年）一套岩石,但还没有测定同位素年龄。

关键词：全岩变质事件 Pb 塞拉利昂 Rb 片麻岩变质岩 sr 等时年龄绿岩带全岩等时线年龄利比里亚太古宙太古代前寒武纪

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澳大利亚国家保护地规划历程及其借鉴

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风景园林 2017年第7期24卷 57-64页

作者：王祝根李晓蕾史蒂芬·j·巴里南京工业大学南京210009 澳大利亚昆士兰州环境与遗产保护部墨尔本3031

澳大利亚国家保护地是基于澳大利亚国土范围内特有的生态、物种和景观资源保护而建立的自然资源保护地,对澳大利亚保护国家生态环境和生物多样性发挥了核心作用。澳大利亚国家保护地体系建设是促进保护地规划系统化发展的重要支撑,其规... 详细信息

澳大利亚国家保护地是基于澳大利亚国土范围内特有的生态、物种和景观资源保护而建立的自然资源保护地,对澳大利亚保护国家生态环境和生物多样性发挥了核心作用。澳大利亚国家保护地体系建设是促进保护地规划系统化发展的重要支撑,其规划建设的过程、经验和思路具有积极的参考价值。通过对发展过程的梳理,将澳大利亚国家保护地规划历程划分为5个阶段,对其建设背景、过程和核心内容做了总结,在此基础上对其国家保护地与国家公园的协同规划矛盾做了分析,对其实施的体系建设策略做了解读。协调好国家自然保护区、国家公园以及国家风景名胜区三者间的关系并在此基础上建立完善的体系建立机制是中国国家公园体制建设的前提条件与关键问题。基于以上思考,本文从指导框架、保护体系、规划合作以及监管机制4个层次对澳大利亚国家自然保护地体系建设的借鉴意义做了总结。

关键词：国家保护地国家公园自然保护区

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专家对VsAT未来的展望

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成都信息工程学院学报 1993年第2期15卷 115-123页

作者： Leonard s.Golding joseph Rinde Andrew j.Viterbi Peter P.Nuspl Richard W.jestin Pierre Bartholome joseph N.Pelton K.M.sundara Murthy barry G.Evans 王春芳

自从VsAT(天线口径非常小的地球站)建立和发展以来,使很多领域不断取得进步。这些领域包括使用超大规模集成技术的技术合成,射频单元集成和批量生产技术、天线的改进和批量生产技术、对新用户和网络协议的支持、用户控制管理选择、高功... 详细信息

自从VsAT(天线口径非常小的地球站)建立和发展以来,使很多领域不断取得进步。这些领域包括使用超大规模集成技术的技术合成,射频单元集成和批量生产技术、天线的改进和批量生产技术、对新用户和网络协议的支持、用户控制管理选择、高功率低损耗放大器、网络监控新技术、发展新应用领域等。在过去的五年中。

关键词： VsAT 地球站射频单元天线口径话音业务网络监控网络协议批量生产网络层协议公共信道

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OCEANOGRAPHIC OBsERVATIONs DURING gale

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journal of Oceanology and Limnology 1987年第4期5卷 309-317页

作者： Larry P.Atkinson,Thomas j.Berger Department of Oceanography Old Dominion University Norfolk VA 23508 U.s.A Department of Oceanography Old Dominion University Norfolk VA 23508 U.s.A

Results of oceanographic observations during gale (Genesis of Atlantic Lows Experiment) are shown. The response of the thermohaline structure of the winds and cooling on the southeast U.s. shelf is described. The mean vertical heat flux over the shelf was calculated at certain stations. However, no attempt was made, in the absense of current meter data, to determine how much of the flux was due to advection or how much due to cooling or other causes.

关键词：卜工 OO IOP OCEANOGRAPHIC OBsERVATIONs DURING gale than Figure OVO OU

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CCs Research Development and Deployment in a Clean Energy Future： Lessons from Australia over the Past Two Decades

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Engineering 2017年第4期3卷 477-484页

作者： Peter j. Cook CO2CRC Ltd.& Peter Cook Centre for Carbon Capture and storage Research The University of Melbourne Melbourne VIC 2010 Australia

There is widespread, though by no means universal, recognition of the importance of carbon capture and storage （CCs） as a carbon mitigation technology. However, the rate of deployment does not match what is required for global temperatures to stay well below 2℃. Although some consider the hurdles to achieving the widespread application of CCs to he almost insurmountable, a more optimistic view is that a great deal is now known about CCs through research, demonstration, and deployment. We know how to do it; we are confident it can be done safely and effectively; we know what it costs; and we know that costs are decreasing and will continue to do so. We also know that the world will need CCs as long as countries, companies, and communities continue to use fossil fuels for energy and industrial processes. What is lacking are the necessary policy drivers, along with a technology-neutral approach to decrease carbon emissions in a cost-effective and timely manner while retaining the undoubted benefits of ready access to reliable and secure electricity and energy-intensive industrial products. In this paper, Australia is used as an example of what has been undertaken in CCs over the past 20 years, particularly in research and demonstration, hut also in international collaboration. Progress in the large-scale deployment of CCs in Australia has been too slow. However, the world＇s largest storage project will soon be operational in Australia as part of the Gorgon liquefied natural gas （LNG） project, and investigations are underway into several large-scale CCs Flagship program opportunities. The organization and progress of the Cooperative Research Centre for Greenhouse Gas Technologies （CO2CRC） Otway Project, which is currently Australia＇s only operational storage project, is discussed in some detail because of its relevance to the commercial deployment of CCs. The point is made that there is scope for building on this Otway activity to investigate more broadly （through the p

关键词： Carbon dioxide Carbon capture and storage Otway Australia

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An Earth-mass planet in a time of COVID-19:KMT-2020-BLG-0414Lb

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Research in Astronomy and Astrophysics 2021年第9期21卷 243-261页

作者： Weicheng Zang Cheongho Han Iona Kondo jennifer C.Yee Chung-Uk Lee Andrew Gould shude Mao Leandro de Almeida Yossi shvartzvald Xiangyu Zhang Michael D.Albrow sun-ju Chung Kyu-Ha Hwang Youn Kil jung Yoon-Hyun Ryu In-Gu shin sang-Mok Cha Dong-jin Kim Hyoun-Woo Kim seung-Lee Kim Dong-joo Lee Yongseok Lee Byeong-Gon Park Richard W.Pogge john Drummond Thiam-Guan Tan joséDias do Nascimento júnior Dan Maoz Matthew T.Penny Wei Zhu Ian A.Bond Fumio Abe Richard barry David P.Bennett Aparna Bhattacharya Martin Donachie Hirosane Fujii Akihiko Fukui Yuki Hirao Yoshitaka Itow Rintaro Kirikawa Naoki Koshimoto Man Cheung Alex Li Yutaka Matsubara Yasushi Muraki shota Miyazaki Greg Olmschenk Clément Ranc Nicholas j.Rattenbury Yuki satoh Hikaru shoji stela Ishitani silva Takahiro sumi Daisuke suzuki Yuzuru Tanaka Paul j.Tristram Tsubasa Yamawaki Atsunori Yonehara Andreea Petric Todd Burdullis Pascal Fouqué Department of Astronomy Tsinghua UniversityBeijing 100084China Department of Physics Chungbuk National UniversityCheongju 28644Republic of Korea Department of Earth and space science Graduate School of ScienceOsaka UniversityToyonakaOsaka 560-0043Japan Center for Astrophysics j Harvard&smithsonian 60 Garden St.CambridgeMA 02138USA Korea Astronomy and space science Institute Daejon 34055Republic of Korea University of science and Technology Korea(UST)217 Gajeong-ro Yuseong-guDaejeon 34113Republic of Korea Max-Planck-Institute for Astronomy Königstuhl 1769117 HeidelbergGermany Department of Astronomy Ohio State University140 W.18th Ave.ColumbusOH 43210USA National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Universidade Federal do Rio Grande do Norte(UFRN) Departamento de F´1sica59078-970NatalRNBrazil Department of Particle Physics and Astrophysics Weizmann Institute of ScienceRehovot 76100Israel University of Canterbury Department of Physics and AstronomyPrivate Bag 4800Christchurch 8020New Zealand school of space Research Kyung Hee UniversityYonginKyeonggi 17104Republic of Korea Possum Observatory PatutahiGisbourneNew Zealand Perth Exoplanet survey Telescope PerthAustralia school of Physics and Astronomy Tel-Aviv UniversityTel-Aviv 6997801Israel Department of Physics and Astronomy Louisiana State UniversityBaton RougeLA 70803 USA Canadian Institute for Theoretical Astrophysics University of Toronto60 St George StreetTorontoON M5S 3H8Canada Institute of Natural and Mathematical sciences Massey UniversityAuckland 0745New Zealand Institute for space-Earth Environmental Research Nagoya UniversityNagoya 464-8601Japan Code 667 NASA Goddard Space Flight CenterGreenbeltMD 20771USA Department of Astronomy University of MarylandCollege ParkMD 20742USA Department of Physics University of AucklandPrivate Bag 92019AucklandNew Zealand Department of Earth and Planetary science Graduate School of ScienceThe University of Tokyo7-3-1 Hongo

We report the discovery of KMT-2020-BLG-0414 Lb,with a planet-to-host mass ratio q2=0.9-1.2×10-5=3-4 q⊕at 1σ,which is the lowest mass-ratio microlensing planet to *** with two other recent discoveries(4?q/q⊕?6),it fills out the previous empty sector at the bottom of the triangular(log s,log q)diagram,where s is the planet-host separation in units of the angular Einstein radiusθ***,these discoveries call into question the existence,or at least the strength,of the break in the mass-ratio function that was previously suggested to account for the paucity of very low-q *** to the extreme magnification of the event,Amax~1450 for the underlying single-lens event,its light curve revealed a second companion with q3~0.05 and|log s3|~1,i.e.,a factor~10 closer to or farther from the host in *** measurements of the microlens parallaxπE and the angular Einstein radiusθE allow estimates of the host,planet and second companion masses,(M1,M2,M3)~(0.3 M⊙,1.0 M⊕,17 Mj),the planet and second companion projected separations,(a⊥,2,a⊥,3)~(1.5,0.15 or 15)au,and system distance DL~1 *** lens could account for most or all of the blended light(I~19.3)and so can be studied immediately with high-resolution photometric and spectroscopic observations that can further clarify the nature of the *** planet was found as part of a new program of high-cadence follow-up observations of high-magnification *** detection of this planet,despite the considerable difficulties imposed by COVID-19(two KMT sites and OGLE were shut down),illustrates the potential utility of this program.

关键词： gravitational lensing:micro

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