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牙龈卟啉单胞菌超声提取物对hPDLSCs表达VEGF的影响

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实用口腔医学杂志 2007年第2期23卷 176-179页

作者：侯建霞邵磊 Peter M Loomer barron hong 北京大学口腔医学院牙周科 100081 东风汽车公司襄樊医院口腔科美国加利福尼亚大学旧金山分校牙周科

目的:观察牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)超声提取物对人牙周膜干细胞(human per-iodontal ligament stem cells,hPDLSCs)表达血管内皮生长因子(vascular endothelial growth factor,VEGF)及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的影响。方法:标准厌氧培养环境下培养Pg,收集细菌菌落,离心,制备细菌的超声提取物,然后以不同的浓度加入hPDLSCs的培养液中。培养6d,分别观察第2、4、6天时hPDLSCs的增殖情况。提取蛋白并用Western Blotting法检测VEGF和MMP-9的表达。结果:与对照组相比,第2天时,10μg/ml和100μg/mlPg轻度促进hPDLSCs的生长,第4天时,10μg/ml和100μg/mlPg则明显抑制hPDLSCs的生长(P<0.05),第6天时,3种浓度的Pg均明显抑制hPDLSCs的生长(P<0.05);1μg/ml和10μg/mlPg上调VEGF的表达,100μg/mlPg明显抑制VEGF的表达;3种浓度的Pg均上调hPDLSCs中MMP-9的表达。结论:作为牙周炎病原菌,大量的Pg可能通过抑制hPDLSCs的生长增殖,抑制局部血管重建以及加速细胞外基质的降解而降低了牙周组织的自我修复能力。

关键词：牙周膜干细胞牙龈卟啉单胞菌血管内皮生长因子基质金属蛋白酶

Ericoid mycorrhizal fungus enhances microcutting rooting of Rhododendron fortunei and subsequent growth

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Horticulture Research 2020年第1期7卷 931-941页

作者： Xiangying Wei Jianjun Chen Chunying Zhang hong Liu Xiuxia Zheng Jingli Mu Institute of Oceanography Minjiang University350108 FuzhouFujian ProvinceChina Department of Environmental Horticulture and Mid-Florida Research and Education Center University of FloridaIFASApopkaFL 32703USA Shanghai Engineering Research Center of Sustainable Plant Innovation Shanghai Botanical Garden200231 ShanghaiChina State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University350002 FuzhouFujian ProvinceChina

Adventitious root(AR)formation is a unique feature of plant reproduction and plays a vital role in crop production as many horticultural and forestry plants are propagated through cuttings.A growing number of reports have shown that microbes,particularly mycorrhizal fungi are able to promote AR formation,but the underlying mechanisms remain largely *** study established an in vitro culture system and investigated AR formation in microcuttings of Rhododendron fortunei *** with Oidiodendron maius barron Om19,an ericoid mycorrhizal fungus *** and precursors involved in the biosynthesis of indole-3-acetic acid(IAA)in Om19 mycelium were ***19 was able to produce a large quantity of tryptophan(Trp)and also indole-3-pyruvate(IPA)and IAA,indicating that IAA biosynthesis in Om19 could be through a Trp-dependent *** inoculation of Om19,ARs were quickly formed in *** related genes were activated in ARs,and Om19 effectively colonized the ***3,a key gene in plant biosynthesis of IAA and genes involved in nitrogen(N)uptake and metabolism,phosphorus(P)uptake were highly *** absorbed significantly greater quantity of mineral nutrients,and their growth was substantially enhanced compared to the control plants without Om19 inoculation.A working model for Om19 enhanced AR formation was *** rapid formation of ARs in cuttings could be due in part to the induction of IAA biosynthesized by Om19 and also attributed to Trp catalyzed biosynthesis of IAA in *** formation,in turn,provided Om19 preferred sites for *** study suggested that in addition to promoting AR formation,Om19 could potentially be used as a new biofertilizer for enhancing production of ericaceous plants,such as blueberry,cranberry,and rhododendron.

关键词： metabolism culture attributed

The integrative analysis of microRNA and mRNA expression in Apis mellifera following maze-based visual pattern learning

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Insect Science 2014年第5期21卷 619-636页

作者： Qiu-hong Qin Zi-Long Wang Liu-Qing Tian Hai-Yan Gan Shao-Wu Zhang Zhi-Jiang Zeng Honeybee Research Institute Jiangxi Agricultural University Nanchang China ARC Center of Excellence in Vision Science Research School of Biology The Australian National University Canberra ACT Australia

The honeybee （Apis mellifera） is a social insect with strong sensory capacity and diverse behavioral repertoire and is recognized as a good model organism for studying the neurobiological basis of learning and memory. In this study, we analyzed the changes in microRNA （miRNA） and messenger RNA （mRNA） following maze-based visual learning using next-generation small RNA sequencing and Solexa/lllumina Digital Gene Expression tag profiling （DGE）. For small RNA sequencing, we obtained 13 367 770 and 13 132 655 clean tags from the maze and control groups, respectively. A total of 40 differentially expressed known miRNAs were detected between these two samples, and all of them were up-regulated in the maze group compared to the control group. For DGE, 5 681 320 and 5 939 855 clean tags were detected from the maze and control groups, respectively. There were a total of 388 differentially expressed genes between these two samples, with 45 genes up-regulated and 343 genes down-regulated in the maze group, compared to the control group. Additionally, the expression levels of 10 differentially expressed genes were confirmed by quantitative reverse transcription polymerase chain reaction （qRT-PCR） and the expression trends of eight of them were consistent with the DGE result, although the degree of change was lower in amplitude. The integrative analysis of miRNA and mRNA expression showed that, among the 40 differentially expressed known miRNAs and 388 differentially expressed genes, 60 pairs of miRNA/mRNA were identified as co-expressed in our present study. These results suggest that both miRNA and mRNA may play a pivotal role in the process of learning and memory in honeybees. Our sequencing data provide comprehensive miRNA and gene expression information for maze-based visual learning, which will facilitate understanding of the molecular mechanisms of honeybee learning and memory.

关键词： Apis mellifera Y-maze learning and memory microRNA DGE differential expression

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一种大功率连续波组件的研制

中国电子科学研究院学报 2018年第1期13卷 67-71页

作者：张永慧沈红汪邦金中国电子科技集团公司第三十八研究所安徽合肥230088

高功率、高效率、高性价比特点的LDMOS功率组件在微波系统中需求广泛,是微波功率系统的核心部件。设计了P波段LDMOS功率组件,论述功率组件的组成与研制,概述功率组件的结构与热设计。使用ADS软件进行仿真优化设计,运用巴伦和阻抗变换方... 详细信息

高功率、高效率、高性价比特点的LDMOS功率组件在微波系统中需求广泛,是微波功率系统的核心部件。设计了P波段LDMOS功率组件,论述功率组件的组成与研制,概述功率组件的结构与热设计。使用ADS软件进行仿真优化设计,运用巴伦和阻抗变换方法设计了功率模块的匹配电路。解决了大功率连续波条件下功率组件设计与可靠性等问题。

关键词：功率组件 LDMOS 热设计

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穿透性角膜移植的手术配合

当代护士（中旬刊） 2009年第7期16卷 49-50页

作者：王燕群肖秀英程梅史小红广东省珠海市人民医院五官科

总结21例应用真空环钻行穿透性角膜移植术的手术配合经验。包括术前准备、术中配合与观察，术后护理等。认为护士应有熟练的配合技巧，术前做好患者的准备和物品准备，术中密切配合，是手术顺利完成的保证。

关键词：角膜移植真空环钻护理

Performance and calibration of quark/gluon-jet taggers using 140 fb^(-1) of pp collisions at √s=13 TeV with the ATLAS detector

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Chinese Physics C 2024年第2期48卷 1-25页

作者： G. Aad B. Abbott K. Abeling N.J. Abicht S.H. Abidi A. Aboulhorma H. Abramowicz H. Abreu Y. Abulaiti B.S. Acharya C. Adam Bourdarios L. Adamczyk S.V. Addepalli M.J. Addison J. Adelman A. Adiguzel T. Adye A.A. Affolder Y. Afik M.N. Agaras J. Agarwala A. Aggarwal C. Agheorghiesei A. Ahmad F. Ahmadov W.S. Ahmed S. Ahuja X. Ai G. Aielli A. Aikot M. Ait Tamlihat B. Aitbenchikh I. Aizenberg M. Akbiyik T.P.A. Åkesson A.V. Akimov D. Akiyama N.N. Akolkar K. Al Khoury G.L. Alberghi J. Albert P. Albicocco G.L. Albouy S. Alderweireldt M. Aleksa I.N. Aleksandrov C. Alexa T. Alexopoulos F. Alfonsi M. Algren M. Alhroob B. Ali H.M.J. Ali S. Ali S.W. Alibocus M. Aliev G. Alimonti W. Alkakhi C. Allaire B.M.M. Allbrooke J.F. Allen C.A. Allendes Flores P.P. Allport A. Aloisio F. Alonso C. Alpigiani M. Alvarez Estevez A. Alvarez Fernandez M. Alves Cardoso M.G. Alviggi M. Aly Y. Amaral Coutinho A. Ambler C. Amelung M. Amerl C.G. Ames D. Amidei S.P. Amor Dos Santos K.R. Amos V. Ananiev C. Anastopoulos T. Andeen J.K. Anders S.Y. Andrean A. Andreazza S. Angelidakis A. Angerami A.V. Anisenkov A. Annovi C. Antel M.T. Anthony E. Antipov M. Antonelli F. Anulli M. Aoki T. Aoki J.A. Aparisi Pozo M.A. Aparo L. Aperio Bella C. Appelt A. Apyan N. Aranzabal S.J. Arbiol Val C. Arcangeletti A.T.H. Arce E. Arena J-F. Arguin S. Argyropoulos J.-H. Arling O. Arnaez H. Arnold G. Artoni H. Asada K. Asai S. Asai N.A. Asbah J. Assahsah K. Assamagan R. Astalos S. Atashi R.J. Atkin M. Atkinson H. Atmani P.A. Atmasiddha K. Augsten S. Auricchio A.D. Auriol V.A. Austrup G. Avolio K. Axiotis G. Azuelos D. Babal H. Bachacou K. Bachas A. Bachiu F. Backman A. Badea P. Bagnaia M. Bahmani A.J. Bailey V.R. Bailey J.T. Baines L. Baines O.K. Baker E. Bakos D. Bakshi Gupta V. Balakrishnan R. Balasubramanian E.M. Baldin P. Balek E. Ballabene F. Balli L.M. Baltes W.K. Balunas J. Balz E. Banas M. Bandieramonte A. Bandyopadhyay S. Bansal L. Barak M. Barakat E.L. Barberio D. Barberis M. Barbero M.Z. Barel K.N. Barends T. Barillari M-S. Barisits T. Barklow P. Baron D.A. Baron Mo The ATLAS Collaboration

The identification of jets originating from quarks and gluons,often referred to as quark/gluon tagging,plays an important role in various analyses performed at the Large Hadron Collider,as Standard Model measurements and searches for new particles decaying to quarks often rely on suppressing a large gluon-induced *** paper describes the measurement of the efficiencies of quark/gluon taggers developed within the ATLAS Collaboration,using √s=13 TeV proton–proton collision data with an integrated luminosity of 140 fb^(-1) collected by the ATLAS *** taggers with high performances in rejecting jets from gluon over jets from quarks are studied:one tagger is based on requirements on the number of inner-detector tracks associated with the jet,and the other combines several jet substructure observables using a boosted decision tree.A method is established to determine the quark/gluon fraction in data,by using quark/gluon-enriched subsamples defined by the jet *** in tagging efficiency between data and simulation are provided for jets with transverse momentum between 500 GeV and 2 TeV and for multiple tagger working points.

关键词： ATLAS JET QUARK GLUON TAGGING