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β-Estradiol 17-acetate enhances the in vitro vitality of endothelial cells isolated from the brain of patients subjected to neurosurgery

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Neural Regeneration Research 2023年第2期18卷 389-395页

作者： Sonia Guzzo Pasquale De Bonis barbara Pavan Luciano Fadiga Department of Neuroscience and Rehabilitation Section of PhysiologyUniversity of FerraraFerraraItaly Center for Translational Neurophysiology of Speech and Communication(CTNSC) Italian Institute of Technology(IIT)FerraraItaly Department of Translational Medicine and for Romagna University of FerraraFerraraItaly

In the current landscape of endothelial cell isolation for building in vitro models of the blood-brain barrier,our work moves towards reproducing the features of the neurovascular unit to achieve glial compliance through an innovative biomimetic coating technology for brain chronic *** hypothesized that the autologous origin of human brain mic rovascular endothelial cells(hBMECs)is the first requirement for the suitable coating to prevent the glial inflammato ry response trigge red by foreign *** re,this study established a new procedure to preserve the in vitro viability of hBMECs isolated from gray and white matter specimens taken from neurosurge ry *** adult hBMECs is generally considered a challenging task due to the difficult survival ex vivo and progressive reduction in proliferation of these *** addition of 10 nMβ-estradiol 17-acetate to the hBMEC culture medium was found to be an essential and discriminating factor promoting adhesion and proliferation both after isolation and thawing,suppo rting the well-known protective role played by estrogens on *** particular,β-estradiol 17-acetate was critical for both freshly isolated and thawed female-derived hBMECs,while it was not necessary for freshly isolated male-derived hBMECs;however,it did countera ct the decay in the viability of the latter after *** tumo r-free hBMECs were thus cultured for up to 2 months and their growth efficiency was assessed befo re and after two periods of *** pite the thermal stress,the hBMECs remained viable and suitable for re-freezing and storage for several *** approach increasing in vitro viability of hBMECs opens new perspectives for the use of cryopreserved autologous hBMECs as biomimetic therapeutic tools,offering the potential to avoid additional surgical sampling for each patient.

关键词： β-estradiol 17-acetate 17β-estradiol cryopreservation gender-specific gray matter human brain microvascular endothelial cells surgical resections vascular protection white matter

Vascular architecture:is it a helpful histopathological biomarker for hepatocellular carcinoma?

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第4期8卷 217-220页

作者： GRIZZI Fabio FRANCESCHINI barbara FIAMENGO barbara RUSSO Carlo DIOGUARDI Nicola Laboratories of Quantitative Medicine Istituto Clinico Humanitas IRCCS20089 RozzanoMilanItaly Department of Pathology Istituto Clinico Humanitas IRCCS20089 RozzanoMilanItaly

Hepatocellular carcinoma(HCC) remains one of the major public health problems throughout the *** originally associated with tumorigenic processes,liver angiogenesis has also been observed in the context of different liver in-flammatory,fibrotic,and ischemic *** we investigate the fractal dimension as a quantitator of non-Euclidean two-dimensional vascular geometry in a series of paired specimens of primary HCC and surrounding non-tumoral tissue,and discuss why this parameter might provide additional information regarding cancer *** application of fractal geometry to the measurement of liver vascularity and the availability of a computer-aided quantitative method can eliminate errors in visual interpretation,and make it possible to obtain closer-to-reality numerals that are compulsory for any measurement process.

关键词： Liver Angiogenesis Cancer Fractals Geometry Biomarkers

Commentary on "The BRICS Council of Exercise and Sport Science(BRICSCESS)——A new era has dawned" that has been published on JSHS

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Journal of Sport and Health Science 2019年第6期8卷 505-506页

作者： barbara E.Ainsworth College of Health Solutions Arizona State University School ofKinesiology Shanghai University of Sport

In 2018,the Journal of Sport and Health Science published an opinion article by Uvinha et all that introduced the readers to the BRICS Council of Exercise and Sport Science(BRICSCESS).BRICS is an acronym coined in 201... 详细信息

关键词： BRICS letter Sport

Innovation in geoprocessing for a Digital Earth

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International Journal of Digital Earth 2018年第1期11卷 3-6页

作者： barbara Hofer Carlos Granell Lars Bernard Interfaculty Department of Geoinformatics-Z_GIS University of SalzburgSalzburgAustria Geospatial Technologies Research Group(GEOTEC) Universitat Jaume ICastellón de la PlanaSpain Chair of Geoinformatics Technische Universität DresdenDresdenGermany

Online geoprocessing,the provision and the use of spatial analysis functionality on the Web,is a powerful technological approach to fully realise the vision of a Digital *** Digital Earth objectives include the integration of information across diverse domains in order to address environmental and societal challenges as well as the interactive and exploratory provision of information to a wide audience(Craglia et al.2008).

关键词： interactive Earth Web

Forensic Readiness: Emerging Discipline for Creating Reliable and Secure Digital Evidence

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Journal of Harbin Institute of Technology(New Series) 2015年第1期22卷 1-8页

作者： barbara Endicott-Popovsky Nicolai Kuntze Carsten Rudolph Institute of Technology University of Washington Fraunhofer Institute of Technology

Traditional approaches to digital forensics reconstruct events within digital systems that often are not built for the creation of evidence; however,there is an emerging discipline of forensic readiness that examines what it takes to build systems and devices that produce digital data records for which admissibility is a requirement. This paper reviews the motivation behind research in this area,a generic technical solution that uses hardware-based security to bind digital records to a particular state of a device and proposed applications of this solution in concrete,practical scenarios. Research history in this area,the notion of secure digital evidence and a technical solution are discussed. A solution to creating hardware-based security in devices producing digital evidence was proposed in 2012. Additionally,this paper revises the proposal and discusses three distinct scenarios where forensic readiness of devices and secure digital evidence are relevant. It shows,how the different requirements of the three scenarios can be realized using a hardware-based solution. The scenarios are:lawful interception of voice communication,automotive black box,precise farming. These three scenarios come from very distinctive application domains. Nevertheless,they share a common set of security requirements for processes to be documented and data records to be stored.

关键词： digital forensics forensic readiness calibration d

STABILIZATION, REFORMATION AND MELTING OF POLY(L-LACTIDE) CRYSTALLITES

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Chinese Journal of Polymer Science 2007年第1期25卷 83-94页

作者： Tai-Yon Cho barbara Heck Gert Strobl Physikalisches Institut Albert-Ludwigs-Universitt Freiburg 79104 FreiburgGermany

The large size of the crystallites in poly（L-lactide） and the low growth rate enable detailed time- and temperaturedependent X-ray scattering studies of the ordering processes to be carded out. A layer located intermediate between crystals and melt-like regions is observed which finally takes on crystalline order. Recrystallization processes during heating change the complete stack structure rather than the crystallites individually and produce voids in the stacks, Establishment of a new stable structure after a temperature jump in the melting range can be followed in time. DSC experiments indicate times of melting of the order of minutes.

关键词： Poly(L-lactide) Polymer crystallization Small angle X-ray scattering Semicrystalline structure.

Science Editing in America:An Overview

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科技与出版 2003年第4期 58-61页

作者： barbara Gastel MD Department of Journalism Texas A&M University 4111 TAMU College Station Texas778434111

In America，science editing appears to be anincreasingly recognized field. In what settings do American science editors work，and what kinds of work do they do? What is their educational background?

关键词： in html Science Editing in America

Diet and functional dyspepsia: Clinical correlates and therapeutic perspectives

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World Journal of Gastroenterology 2020年第5期26卷 456-465页

作者： Marcella Pesce Martina Cargiolli Sara Cassarano barbara Polese barbara De Conno Laura Aurino Nicola Mancino Giovanni Sarnelli Department of Clinical Medicine and Surgery “Federico II”University of NaplesNaples 80131Italy GI Physiology Unit University College London HospitalLondon NW12BUUnited Kingdom

Hypervigilance and symptoms anticipation,visceral hypersensitivity and gastroduodenal sensorimotor abnormalities account for the varied clinical presentation of functional dyspepsia(FD)*** patients recognize meals as the main triggering factor;thus,dietary manipulations often represent the first-line management strategy in this cohort of ***,scarce quality evidence has been produced regarding the relationship between specific foods and/or macronutrients and the onset of FD symptoms,resulting in nonstandardized nutritional *** dietary advises are indeed empirical and often lead to exclusion diets,reinforcing in patients the perception of“being intolerant”to food and self-perpetuating some of the very mechanisms underlying dyspepsia physiopathology(i.e.,hypervigilance and symptom anticipation).Clinicians are often uncertain regarding the contribution of specific foods to dyspepsia physiopathology and dedicated professionals(i.e.,dietitians)are only available in tertiary referral *** in turn,can result in nutritionally unbalanced diets and could even encourage restrictive eating behaviors in severe *** this review,we aim at evaluating the relationship between dietary habits,macronutrients and specific foods in determining FD *** will provide an overview of the evidence-based nutritional approach that should be pursued in these patients,providing clinicians with a valuable tool in standardizing nutritional advises and discouraging patients from engaging into indiscriminate food exclusions.

关键词： Functional dyspepsia Dietary habits Food intolerances Fermentable oligosaccharides,disaccharides,monosaccharides and polyols Gluten-sensitivity Diet

The influence of time and temperature on molecular gut content analysis： Adalia bipunctata fed with Rhopalosiphum padi

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Insect Science 2007年第5期14卷 353-358页

作者： STUART MCMILLAN ANNA-KARIN KUUSK ANNA CASSELLUNDHAGEN barbara EKBOM Department of Ecology Swedish University of Agricultural Sciences Box 7044 750 07 Uppsala Sweden

Gut content analysis is a useful tool when studying arthropod predator-prey interactions. We used polymerase chain reaction （PCR） technique to examine how detection of prey DNA in the gut content of predators was influenced by digestion time and temperature. Such knowledge is critical before applying PCR-based gut content analysis to field collected predators. Larvae of the two-spotted ladybeetle （Adalia bipunctata L.） were fed with the bird cherry-oat aphid （Rhopalosiphum padi L.） at either 21℃ or 14℃. After consuming one aphid, the predators were allowed to digest the prey for a range of time periods up to 24 hours. The influence of temperature on A. bipunctata feeding behavior was also recorded. From the fed larvae, total DNA was extracted and PCR reactions with R. padi specific primers were run. The number ofA. bipunctata that tested positive for R. padi DNA was negatively related to the length of digestion time. Temperature influenced larval feeding behavior but did not have a significant effect on R. padi DNA detection. After pooling the data from both temperature treatments we estimated the time point when R. padi DNA could be amplified from 50% of the fed A. bipunctata by PCR to be 4.87 hours. With such a rapid decrease in prey DNA detection success, positive PCR reactions will most likely be the result of predation events occurring shortly before capture. If a defined digestion temperature range has proven not to influence prey detection, PCR data obtained from predators collected within that particular range can be interpreted in the same way.

关键词： Adalia bipunctata aphidophagous predator prey DNA detection success gut content analysis PCR Rhopalosiphum padi