检索结果-南通市图书馆

The double mutations of acvr2aa and acvr2ba leads to muscle hypertrophy in zebrafish

Aquaculture and Fisheries 2023年第6期8卷 706-712页

作者： Jinyuan Che Cunjie Hu Qi Wang Chunxin Fan Yufeng Si Xiaoling Gong baolong bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China

Activin A receptor,type II(Acvr2)is a member of the transforming growth factor beta receptor family and can function as a negative regulator of skeletal muscle mass.Acvr2 plays an important role in regulating muscle development that can inhibit skeletal muscle growth in mice.However,there is very little research reported on the function of acvr2 in muscle development of teleost.In this study,we analyzed the effect of acvr2aa and acvr2ba on muscle development in zebrafish.Growth rates of WT and acvr2a^(-/-b-/-)􀀀were measured from juvenile stage to adult stage.In addition,effects of acvr2 on skeletal muscle were tested in histological,protein and molecular levels.As a result,acvr2a^(-/-b-/-)􀀀exhibited a wider body trunk than WT and showed a significant increase in body weight and width from two months old.Histological analysis of skeletal muscle indicated that the size of muscle fiber in acvr2a^(-/-b-/-)􀀀(female:1809±123μm^(2);male:2261±130μm^(2))was larger than that in WT(709.8±49μm^(2);815±53μm^(2)).In addition,western blot of fast MyLc protein showed the protein synthesis of acvr2a^(-/-b-/-)􀀀are increased.Besides,Histological analysis of heart showed the ventricle area is aslo increased in acvr2a^(-/-b-/-)􀀀.Our results demonstrated acvr2 attends the development of muscle fiber and will cause muscle hypertrophy when they were knocked out in zebrafish.In conclusion,acvr2 in zebrafish can control the development of muscle fibers during posthatch growth.

关键词： acvr2 Muscle fiber Hypertrophy Zebrafish Skeletal muscle growth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

omd基因对斑马鱼骨骼矿化的影响

引用

上海海洋大学学报 2024年第1期33卷 30-42页

作者：王琪车金远鲍宝龙上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306

omd基因编码骨调蛋白,可以调控人类骨骼的矿化。目前关于omd基因对鱼类骨骼矿化的作用尚不清楚。为了探究omd基因对鱼类骨骼的影响,调查了omd基因在斑马鱼不同发育阶段和不同组织的表达,并利用CRISPR/Cas9基因编辑技术,成功构建斑马鱼om... 详细信息

omd基因编码骨调蛋白,可以调控人类骨骼的矿化。目前关于omd基因对鱼类骨骼矿化的作用尚不清楚。为了探究omd基因对鱼类骨骼的影响,调查了omd基因在斑马鱼不同发育阶段和不同组织的表达,并利用CRISPR/Cas9基因编辑技术,成功构建斑马鱼omd基因突变体(omd^(-/-))。结果表明:omd基因在斑马鱼体内随着发育表达量逐渐增加,在脊柱和肌肉中表达较高;相比野生型斑马鱼,omd^(-/-)品系斑马鱼的骨骼形态和尾部肌肉结构未发现明显变化,脊柱的钙含量下调59.81%;与野生型相比,omd^(-/-)纯合敲除品系斑马鱼平均游泳距离下降33.93%,平均游泳速度下降39.44%,相对静止的时间增加88.26%,游泳能力下降。这些结果表明,omd基因缺失影响斑马鱼脊柱的矿化,并在一定程度上影响了斑马鱼的活动能力。

关键词： omd 斑马鱼骨骼骨骼矿化游泳

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

aldh3a2a敲除对斑马鱼体色的影响

引用

上海海洋大学学报 2024年第2期33卷 317-326页

作者：卢培钰冯毅栋鲍宝龙上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306

本研究探究了aldh3a2a在斑马鱼色素细胞发育中的意义及其对醛代谢和肝脏健康的影响。利用CRISPR/Cas9技术,成功构建aldh3a2a敲除纯合突变家系,导致黑色素细胞、黄色素细胞和虹彩细胞数量减少,csf1ra、pnp4a、itk下调,黑色素合成通路关... 详细信息

本研究探究了aldh3a2a在斑马鱼色素细胞发育中的意义及其对醛代谢和肝脏健康的影响。利用CRISPR/Cas9技术,成功构建aldh3a2a敲除纯合突变家系,导致黑色素细胞、黄色素细胞和虹彩细胞数量减少,csf1ra、pnp4a、itk下调,黑色素合成通路关键基因mitfa、tyrp1b和kita下调。转录组分析揭示了基因表达谱系的变化,差异基因scd、lpla和aox5在类固醇生物合成途径中下调,指示aldh3a2a对类固醇生物合成的关键作用。对视黄醇代谢通路的转录组分析和qpcr联合研究结果表明,aldh3a2a可以调控raldh2、rxrab以及aox5,表明其对视黄酸受体的信号传导有一定影响,同时aldh3a2a可以调控钾离子通道蛋白kcns3b,指示其对色素细胞的影响与钾离子信号传导有关。GO和KEGG富集分析发现突变体的细胞周期、同源重组、RNA降解、RNA转运、p53信号通路、真核生物核糖体生物发生、DNA复制、碱基切除修复、氨基酰基-tRNA生物合成、细胞质DNA传感通路、类固醇生物合成、错配修复、类固醇激素生物合成和醛酸盐代谢等通路也有着广泛影响。此外,aldh3a2a基因敲除会导致体内醛积累明显,产生细胞毒性进而引发肝脏体积异常增大、肝脏内出现空泡,体质量增加的表征,类似于Sjögren-Larsson综合征的症状,揭示了aldh3a2a在斑马鱼体内的作用及其与人类遗传疾病的相关性,将有助于更深入地了解其在斑马鱼和人类中的功能和潜在的治疗靶点。

关键词： aldh3a2a基因敲除色素细胞视黄醇代谢通路视黄酸代谢

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RseB影响溶藻弧菌感染花鲈的能力

引用

上海海洋大学学报 2024年第1期33卷 9-17页

作者：周家宇姜维王雨果陈小辉罗土炎鲍宝龙上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306 福建闽威实业股份有限公司福建福鼎355200 福建省农业科学院农业质量标准与检测技术研究所福建福州350003

为研究RseB基因缺失对溶藻弧菌(Vibrio alginolyticus)感染花鲈(Lateolabrax maculatus)能力的影响,评估花鲈感染后的LD_(50)、定殖能力和免疫响应。结果显示,溶藻弧菌野生株LD_(50)为2.013×10^(5) cfu/mL,RseB缺失溶藻弧菌LD_(50)为5.526×10^(5) cfu/mL,RseB缺失溶藻弧菌毒力下降63.6%;溶藻弧菌主要在花鲈的鳃部、肠道和皮肤定殖,相对野生菌株,RseB缺失溶藻弧菌定殖肠道的空泡化程度更低,鳃组织结构的损坏程度更低;分别对野生株与RseB缺失溶藻弧菌感染的花鲈头肾组织进行转录组测序,发现与免疫相关的差异表达基因一共有236个,其中包括了122个显著上调的免疫基因和114个显著下调的免疫基因,大部分显著富集在Th1和Th2细胞分化、B细胞受体信号通路、T细胞受体信号通路等特异性免疫通路上,部分基因显著富集在与细胞凋亡相关的通路以及与炎症相关的NF-kappa B信号通路中。结果表明:RseB缺失溶藻弧菌能引起宿主细胞的免疫反应且毒力明显降低。

关键词：溶藻弧菌花鲈 Rse基因致病性免疫响应

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

暗纹东方鲀组织内TTX含量与钠离子通道基因表达特征分析

引用

上海海洋大学学报 2024年第2期33卷 308-316页

作者：王婧王润林梦娇王亚宁鲍宝龙龚小玲上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学水产科学国家级实验教学示范中心上海201306 中国水产科学研究院黄海水产研究所山东青岛266071 青岛大学生命科学学院山东青岛266071

为了探究暗纹东方鲀(Takifugu obscurus)组织内河鲀毒素(Tetrodotoxin,TTX)含量与含毒组织电压门控钠离子通道基因表达特征。分别采用酶联免疫法(ELISA)测定暗纹东方鲀肝脏、心脏等8个组织TTX含量,实时荧光定量(RT-qPCR)方法检测电压门... 详细信息

为了探究暗纹东方鲀(Takifugu obscurus)组织内河鲀毒素(Tetrodotoxin,TTX)含量与含毒组织电压门控钠离子通道基因表达特征。分别采用酶联免疫法(ELISA)测定暗纹东方鲀肝脏、心脏等8个组织TTX含量,实时荧光定量(RT-qPCR)方法检测电压门控钠离子通道α亚基基因家族成员scn1aa,scn1ab,scn4aa,scn4ab,scn5aa,scn5ab,scn8aa,scn8ab(以下简称“电压门控钠离子通道基因”)在各组织中的相对表达特征。结果显示:各组织中TTX含量由高到低为脑(9.521±2.816)μg/g、心脏(4.271±1.129)μg/g、鳃(1.586±0.527)μg/g、肌肉(1.494±0.938)μg/g、肝脏(0.913±0.206)μg/g、皮肤(0.902±0.235)μg/g、肠道(0.894±0.215)μg/g和眼(0.864±0.287)μg/g,脑、心脏组织为弱毒性,肝脏、皮肤、肌肉、眼、肠道、鳃组织为微毒性。RT-qPCR结果显示scn1aa、scn1ab、scn4ab、scn5aa、scn5ab、scn8aa、scn8ab基因均在肝脏组织的相对表达量最高,scn4aa基因仅在肌肉组织表达,皮肤组织仅有scn4ab基因表达,且scn4ab基因在所有检测组织中均有表达,表达具有广泛性。钠离子通道基因在斑马鱼和暗纹东方鲀组织表达特征存在差异。

关键词：暗纹东方鲀电压门控钠离子通道河鲀毒素荧光定量PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Population genetic structure in wild and aquaculture populations of Hemibarbus maculates inferred from microsatellites markers

引用

Aquaculture and Fisheries 2017年第2期2卷 88-93页

作者： Linlin Li Hungdu Lin Wenqiao Tang Dong Liu baolong bao Jinquan Yang Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China The Affiliated School of National Tainan First Senior High School Tainan 701TaiwanChina

The objective of this study was to investigate 4 aquaculture populations Shanghai(SH),Hangzhou(HZ),Kaihua(KH)and Xianju(XJ)and one wild population Yingshan(YS)of spotted barbell(Hemibarbus maculates)to assess their genetic diversity level and investigate the genetic structure of the populations.The dendrogram and STRUCTURE revealed that the populations XJ,KH,and HZ jointly formed one cluster,to which the populations SH and YS were sequentially adhered.The genetic diversity of the cultured populations maintained better,possible due to favourable hatchery conditions that decreased the effect of environmental selection present in wild populations.The results of the present study will contribute to the management of spotted barbell genetic resources,but also demonstrates how the genetic diversity of freshwater species is vulnerable to human activity.

关键词： Population structure jointly

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic and morphological differences between yellowtail kingfish (Seriola lalandi) from the Bohai Sea, China and the Southern Ocean, Australia

引用

Aquaculture and Fisheries 2021年第3期6卷 260-266页

作者： Qiaoyun Ai Longlong Sang Hongxin Tan Xuxiong Huang baolong bao Chenhong Li Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution Shanghai Ocean UniversityShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquaculture Shanghai Ocean UniversityShanghai Science and Technology CommitteeShanghai201306China Key Laboratory of Freshwater Aquatic Genetic Resources Shanghai Ocean UniversityMinistry of Agriculture and Rural Affairs of PR ChinaShanghai201306China Shanghai University Knowledge Service Platform Shanghai Ocean UniversityAquatic Animal Breeding CenterZF1206Shanghai201306China

The aquaculture of yellowtail kingfish(Seriola lalandi)has expanded around the globe,including China.Genetic resource of Chinese S.lalandi urgently needs to be assessed for improving production.Here,we collected wild S.lalandi samples from the Bohai Sea,China and evaluated its genetic diversity based on 17,690 nuclear loci.A population from the Southern Ocean,Australia was used for comparison.The analyses showed that the Chinese and Australian S.lalandi formed two completely distinct clusters and there was no genetic introgression from the Australian S.lalandi into the Chinese S.lalandi population.The genetic diversity is slightly lower,but comparable in Chinese versus Australian yellowtail kingfish(Chinese population,expected heterozygosity:0.19,observed heterozygosity:0.19,nucleotide diversity:0.19±0.09;Australian population,expected heterozygosity:0.23,observed heterozygosity:0.22,nucleotide diversity:0.22±0.11).Overall,our results indicated that Chinese S.lalandi could be a potential subject for genetic breeding programs.We also investigated morphological characters and developed molecular markers for population identification.Comparison of meristic characters between the Chinese population and the Australian population revealed that main shape difference were in the number of dorsal spines,dorsal fin rays and the number of upper gill rakers.Geometric morphology based on eight landmarks also revealed significant difference between the two populations including the distance between the tip of snout to origin of pelvic fin and the distance between the tip of snout to origin of pectoral fin.These morphological characters can be used for easy identification of the Chinese S.lalandi.

关键词： Seriola lalandi Population structure Genetic diversity Geometric morphology Molecular markers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gonadal development and silvering of the Japanese Eel(Anguilla japonica)in the Yangtze River during downstream migration

引用

Aquaculture and Fisheries 2017年第4期2卷 173-178页

作者： Xiaoling Gong Dehai Wang baolong bao Qian Zhang Xinglong Jiang Liping Liu The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China Engineering Research Center of Eel Industrial Technology Ministry of EducationJimei UniversityXiamen 361021China

The Yangtze River and its branches are important habitats for the Japanese Eel(Anguilla japonica),however,there are few data on gonadal development and silvering indices of eels during the downstream spawning migration.This study investigates gonadal development in 121 Japanese eels caught downstream from the Jingjiang Reach of the Yangtze River in October 2012.We found that in females the ovaries of most of the out-migrating population developed earlier than the testes in males,that is,the ovaries reached stage 3(F3)or early stage 4(eF4),while the testes were at stage 2(M2)or stage 3(M3).Gonadal development in eels collected in 2012 was earlier than that of samples caught from the same region in 2007e2008 and from the Pearl River Estuary in 1972e1973.The average age,body size and gonadal-somatic index of females were significantly greater than those in males.For both females and males,there were no significant differences between the two stages of gonadal development in any of the morphological indices,which indicates that gonadal development of the Japanese Eel is independent of body size.These results help us to understand the spawning population of Japanese Eels in the Yangtze River.

关键词： Japanese eel Anguilla japonica Spawning migration Gonadal development Morphological indices

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The role of nitric oxide and neuronal nitric oxide synthase in zebrafish(Danio rerio)shoaling

引用

Aquaculture and Fisheries 2021年第6期6卷 565-573页

作者： Rachit Penglee Lei Gao Yajuan Huang Liping Liu Sukkrit Nimitkul baolong bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China Department of Aquaculture Faculty of FisheriesKasetsart UniversityChatuchakBangkok10900Thailand

Nitric oxide(NO)-the product of arginine metabolism catalyzed by nitric oxide synthases(NOS)-is a well-known neurotransmitter which plays an important role in metabolism and amino acid transportation in the nervous system.In particular,it can inhibit monoamine neurotransmitter transportation which affects animal behavior,especially social behavior.Shoaling-is a one kind of social behavior.It is a behavior that individual fish choose to join with their group within two factors;food and predation risk.Shoaling fish has quickly responded to predator and increased the change in feeding competition.In addition,shoaling also effect to stress response on stock density of aquaculture system.The effect of NO molecular signaling on the dopamine pathway was investigated using zebrafish(Danio rerio)as a model organism.Our aim was to understand the role of NOS and NO in shoaling behavior,which is typical of zebrafish.The concentration of NO in the zebrafish brain was modulated using a knockout for the neuronal NOS gene,and NO production was induced through treatment with L-arginine.The existence of NO in the zebrafish brain was confirmed by using a fluorescent probe.Dopamine concentration in the brain was measured by UPLC tandem mass spectrometer.We measured shoaling cohesion of all individual fish of D.rerio,using average distance between all pairs of fish(nearest neighbor distance)and analyzed tracking by Zebralab ViewPoint software.Collectively,our results suggest that a lower level of NO was associated with a higher level of dopamine,which in turn leads to the shoaling behavior.

关键词： Nitric oxide Neuronal nitric oxide synthase Shoaling Dopamine transporter Neurotransmitter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Eye location, cranial asymmetry, and swimming behavior of different variants of Solea senegalensis

引用

Aquaculture and Fisheries 2020年第4期5卷 182-186页

作者： Jubin Xing Zhonghe Ke Liping Liu Chenhong Li Xiaoling Gong baolong bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China Fishery Technical Extension Station of Zhuji City ZhujiZhejiang311800China

Many flatfish species,such as Scophthalmus maximus,Paralichthys olivaceus,Solea senegalensis,are extensively cultured worldwide.In flatfishes,eye migration varies among different species and even within the same species.The genetic mechanism underlying eye migration is still unclear,and it is the key to improve metamorphosis in flatfish aquaculture and understand flatfish evolution.In this study,the high frequency of eye location variants in an artificial S.senegalensis population allowed us to investigate the eye preference during migration;completeness of the eye migration;and other related left-right asymmetric characteristics,such as asymmetrical skull remodeling and lying down on one side.The different frequencies of eye preference during migration,completeness of the eye migration,and settling side suggest that they are determined by different genotypes and imply independent evolutionary events during flatfish evolution.We found that the swimming time may be related to the completeness of eye migration,and further the migration of the eye,the more time the fish spent at the sea bottom.The left-right asymmetric degrees of not only the frontal bones and lateral ethmoids but also the jaw bone,including the premaxillary,maxillary,and dentary bones,depend on the completeness of eye migration.Finally,we proposed a hypothesis for the asymmetric origin of flatfish that provides reasonable explanations for the selective advantages of primitive flatfish species.

关键词： Flatfish Eye migration Left/right asymmetry Senegalese sole

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：