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Agronomical selection on loss-of-function of GIGANTEA simultaneously facilitates soybean salt tolerance and early maturity

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Journal of Integrative Plant Biology 2022年第10期64卷 1866-1882页

作者： Lidong Dong Zhihong Hou Haiyang Li Zhaobo Li Chao Fang Lingping Kong Yongli Li Hao Du Tai Li Lingshuang Wang Milan He Xiaohui Zhao Qun Cheng Fanjiang Kong baohui liu Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou Higher Education Mega Center230 Waihuanxi RoadGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqingChina National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China College of Agriculture Jilin Agricultural Science and Technology UniversityJilin 132101China

Salt stress and flowering time are major factors limiting geographic adaptation and yield productivity in soybean(Glycine max).Although improving crop salt tolerance and latitude adaptation are essential for efficient agricultural production,whether and how these two traits are integrated remains largely unknown.Here,we used a genome-wide association study to identify a major salt-tolerance locus controlled by E2,an ortholog of Arabidopsis thaliana GIGANTEA(GI).Loss of E2 function not only shortened flowering time and maturity,but also enhanced salt-tolerance in soybean.E2 delayed soybean flowering by enhancing the transcription of the core flowering suppressor gene E1,thereby repressing Flowering Locus T(FT)expression.An E2 knockout mutant e2^(CR) displayed reduced accumulation of reactive oxygen species(ROS)during the response to salt stress by releasing peroxidase,which functions in ROS scavenging to avoid cytotoxicity.Evolutionary and population genetic analyses also suggested that loss-of-function e2 alleles have been artificially selected during breeding for soybean adaptation to high-latitude regions with greater salt stress.Our findings provide insights into the coupled selection for adaptation to both latitude and salt stress in soybean;and offer an ideal target for molecular breeding of early-maturing and salt-tolerant cultivars.

关键词： floweringtime GIGANTEA ROS saltstress soybean

Molecular mechanisms for the photoperiodic regulation of flowering in soybean

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Journal of Integrative Plant Biology 2021年第6期63卷 981-994页

作者： Xiaoya Lin baohui liu James LWeller Jun Abe Fanjiang Kong Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510642China Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China School of Natural Sciences University of TasmaniaHobartTasmania 7001Australia Research Faculty of Agriculture Hokkaido UniversitySapporo 060-8589Japan

Photoperiodic flowering is one of the most important factors affecting regional adaptation and yield in soybean(Glycine max). Plant adaptation to long-day conditions at higher latitudes requires early flowering and a reduction or loss of photoperiod sensitivity;adaptation to short-day conditions at lower latitudes involves delayed flowering, which prolongs vegetative growth for maximum yield potential. Due to the influence of numerous major loci and quantitative trait loci(QTLs), soybean has broad adaptability across latitudes. Forward genetic approaches have uncovered the molecular basis for several of these major maturity genes and QTLs. Moreover, the molecular characterization of orthologs of Arabidopsis thaliana flowering genes has enriched our understanding of the photoperiodic flowering pathway in soybean. Building on early insights into the importance of the photoreceptor phytochrome A, several circadian clock components have been integrated into the genetic network controlling flowering in soybean: E1, a repressor of FLOWERING LOCUS T orthologs, plays a central role in this network. Here, we provide an overview of recent progress in elucidating photoperiodic flowering in soybean, how it contributes to our fundamental understanding of flowering time control, and how this information could be used for molecular design and breeding of high-yielding soybean cultivars.

关键词： molecular-designed breeding photoperiodic flowering soybean

Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35

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Neural Regeneration Research 2012年第9期7卷 652-658页

作者： Mingmin Yan Shanping Mao Huimin Dong baohui liu Qian Zhang Gaofeng Pan Zhiping Fu Department of Neurology Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China Department of Neurosurgery Renmin Hospital Wuhan University Wuhan 430060 Hubei Province China

PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.

关键词： Schisandrin B PC12 cells amyloid β-protein 25-35 amyloid precursor protein vacuolar protein sorting 35 neural protection

神经内镜联合显微镜微血管减压术治疗原发性三叉神经痛的疗效观察

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中国临床解剖学杂志 2024年第2期42卷 208-212页

作者：沈江江刘志红刘宝辉湖北省武穴市第一人民医院湖北武穴435400 武汉大学人民医院湖北武汉430060

目的探讨神经内镜联合显微镜行微血管减压术治疗原发性三叉神经痛的临床疗效。方法回顾分析78例行微血管减压术治疗的原发性三叉神经痛患者资料,其中常规显微镜组(A组)36例,神经内镜联合显微镜微血管减压术(B组)42例,分析比较两组术中... 详细信息

目的探讨神经内镜联合显微镜行微血管减压术治疗原发性三叉神经痛的临床疗效。方法回顾分析78例行微血管减压术治疗的原发性三叉神经痛患者资料,其中常规显微镜组(A组)36例,神经内镜联合显微镜微血管减压术(B组)42例,分析比较两组术中责任血管发现率、手术并发症及术后疗效。结果B组责任血管发现率(95.2%)明显高于A组责任血管发现率(80.6%)、治愈率(97.6%)高于A组(83.3%),两者差异均有统计学意义(P0.05)。结论神经内镜联合显微镜微血管减压术治疗三叉神经痛有助于避免责任血管遗漏,提高责任血管发现率和手术治愈率。

关键词：原发性三叉神经痛微血管减压术神经内镜显微镜

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大数据技术在食品安全监管风险预警中存在的问题以及解决措施

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食品安全导刊 2024年第3期 9-11页

作者：刘宝慧烟台市蓬莱区市场监督管理局执法大队烟台蓬莱265600

食品安全监管风险预警是食品安全监管中的重要内容。由于食品安全监管中存在数据来源渠道不畅、数据类型多样、数据价值难以挖掘等问题,在现行风险预警模式下,将大数据技术引入食品安全风险监管风险预警工作中,能有效提升预警效能。本... 详细信息

食品安全监管风险预警是食品安全监管中的重要内容。由于食品安全监管中存在数据来源渠道不畅、数据类型多样、数据价值难以挖掘等问题,在现行风险预警模式下,将大数据技术引入食品安全风险监管风险预警工作中,能有效提升预警效能。本文在阐述大数据技术在食品安全风险评估中的优势基础上,分析了大数据技术在食品安全风险监管中存在的问题,并提出相应的解决措施。

关键词：大数据技术食品安全监管风险预警

Two soybean homologues of TERMINAL FLOWER 1 control flowering time under long day conditions

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The Crop Journal 2023年第3期11卷 704-712页

作者： Lingshuang Wang Chun Lin Bohui Li Tong Su Shichen Li Haiyang Li Fanglei He Chuanjie Gou Zheng Chen Yanan Wang Jun Qin baohui liu Fanjiang Kong Lin Yue Sijia Lu Chao Fang Guangdong Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina College of Agriculture and Biotechnology Yunnan Agricultural UniversityKunming 650201YunnanChina National Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Cereal&Oil Crop Hebei Academy of Agricultural and Forestry SciencesKey Laboratory of Crop Genetics and Breeding of HebeiShijiazhuang 050035HebeiChina

Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean.After whole genome duplications,about 75%of genes being represented by multiple copies in soybean.There are four TERMINAL FLOWER 1(TFL1)genes in soybean,and the TFL1b(Dt1)has been characterized as the determinant of stem growth habit.The function of other TFL1 homologs in soybean is still unclear.Here,we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants.We investigated that TFL1c and TFL1d could physically interact with the b ZIP transcription factor FDc1 and bind to the promoter of APETALA1a(AP1a).RNA-seq and q RT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean.The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions.Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield.

关键词： Soybean TFL1c TFL1d Flowering time

Perspectives on the Application of Genome-Editing Technologies in Crop Breeding

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Molecular Plant 2019年第8期12卷 1047-1059页

作者： Kai Hua Jinshan Zhang Jose Ramon Botella Changle Ma Fanjiang Kong baohui liu Jian-Kang Zhu Shanghai Center for Plant Stress Biology CAS Center of Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China School of Agriculture and Food Sciences University of QueenslandBrisbaneQLD 4072Australia Shandong Provincial Key Lab of Plant Stress School of Life SciencesShandong Normal UniversityJinanChina School of Life Sciences Guangzhou UniversityGuangzhouChina Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA

Most conventional and modern crop-improvement methods exploit natural or artificially induced genetic variations and require laborious characterization of the progenies of multiple generations derived from time-consuming genetic crosses.Genome-editing systems,in contrast,provide the means to rapidly modify genomes in a precise and predictable way,making it possible to introduce improvements directly into elite varieties.Here,we describe the range of applications available to agricultural researchers using existing genome-editing tools.In addition to providing examples of genome-editing applications in crop breeding,we discuss the technical and social challenges faced by breeders using genome-editing tools for crop improvement.

关键词： genome editing crop breeding mutations base editing plants

Parallel selection of distinct Tof5 alleles drove the adaptation of cultivated and wild soybean to high latitudes

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Molecular Plant 2022年第2期15卷 308-321页

作者： Lidong Dong Qun Cheng Chao Fang Lingping Kong Hui Yang Zhihong Hou Yongli Li Haiyang Nan Yuhang Zhang Qingshan Chen Chunbao Zhang Kun Kou Tong Su Lingshuang Wang Shichen Li Haiyang Li Xiaoya Lin Yang Tang Xiaohui Zhao Sijia Lu baohui liu Fanjiang Kong Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510405China College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing 163000China Department of Agriculture Northeast Agricultural UniversityHarbin 150000China Soybean Research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China National Center for Soybean Improvement National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing 210095China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081China

Photoperiod responsiveness is a key factor limiting the geographic distribution of cultivated soybean and its wild ancestor.In particular,the genetic basis of the adaptation in wild soybean remains poorly understood.In this study,by combining whole-genome resequencing and genome-wide association studies we identified a novel locus,Time of Flowering 5(Tof5),which promotes flowering and enhances adaptation to high latitudes in both wild and cultivated soybean.By genomic,genetic and transgenic analyses we showed that Tof5 en-codes a homolog of Arabidopsis thaliana FRUITFULL(FUL).Importantly,further analyses suggested that different alleles of Tof5 have undergone parallel selection.The Tof5H1 allele was strongly selected by humans after the early domestication of cultivated soybean,while Tof5H2 allele was naturally selected in wild soybean,and in each case facilitating adaptation to high latitudes.Moreover,we found that the key flowering repressor E1 suppresses the transcription of Tof5 by binding to its promoter.In turn,Tof5 physically associates with the promoters of two important FLOWERING LOCUS T(FT),FT2a and FT5a,to upregulate their transcription and promote flowering under long photoperiods.Collectively,ourfindings provide insights into how wild soybean adapted to high latitudes through natural selection and indicate that cultivated soybean underwent changes in the same gene but evolved a distinct allele that was artificially selected after domestication.

关键词： soybean wild natural selection artificially selection latitude adaptation flowering time

A recent retrotransposon insertion of J caused E6 locus facilitating soybean adaptation into low latitude

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Journal of Integrative Plant Biology 2021年第6期63卷 995-1003页

作者： Chao Fang Jun liu Ting Zhang Tong Su Shichen Li Qun Cheng Lingping Kong Xiaoming Li Tiantian Bu Haiyang Li Lidong Dong Sijia Lu Fanjiang Kong baohui liu Innovative Center of Molecular Genetics and Evolution School of Life SciencesGuangzhou UniversityGuangzhou 510006China The Innovative Academy of Seed Design Key Laboratory of Soybean Molecular Design BreedingNortheast Institute of Geography and Agroecologythe Chinese Academy of SciencesHarbin 150000China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical Gardenthe Chinese Academy of SciencesGuangzhou 510650China National Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China

Soybean(Glycine max) is an important legume crop that was domesticated in temperate regions.Soybean varieties from these regions generally mature early and exhibit extremely low yield when grown under inductive short-day(SD) conditions at low latitudes. The long-juvenile(LJ) trait, which is characterized by delayed flowering and maturity,and improved yield under SD conditions, allowed the cultivation of soybean to expand to lower latitudes. Two major loci control the LJ trait: J and E6. In the current study, positional cloning, sequence analysis, and transgenic complementation confirmed that E6 is a novel allele of J, the ortholog of Arabidopsis thaliana EARLY FLOWERING 3(ELF3). The mutant allele e6^(PG), which carries a Ty1/Copia-like retrotransposon insertion, does not suppress the legume-specific flowering repressor E1, allowing E1 to inhibit Flowering Locus T(FT) expression and thus delaying flowering and increasing yields under SD conditions. The e6^(PG)allele is a rare allele that has not been incorporated into modern breeding programs.The dysfunction of J might have greatly facilitated the adaptation of soybean to low latitudes. Our findings increase our understanding of the molecular mechanisms underlying the LJ trait and provide valuable resources for soybean breeding.

关键词： E6 long-juvenile(LJ)trait J Ty1/copia-like retrotransposon