检索结果-南通市图书馆

An Evaluation of Metronidazole Degradation in a Plasma-Assisted Rotating Disk Reactor Coupled with TiO2 in Aqueous Solution

在线全文

学校读者我要写书评

暂无评论

Engineering 2021年第11期7卷 1603-1610页

作者： Yong Cai Guang-Wen Chu Yong Luo Meng-jun Su bao-ju wang bao-Chang Sun Jian-Feng Chen State Key Laboratory of Organic–Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Research Center of the Ministry of Education for High-Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China

Pollution involving pharmaceutical components in bodies of water is an increasingly serious environmental *** discharge for the degradation of antibiotics is an emerging technology that may be relevant toward addressing this *** this work,a plasma-assisted rotating disk reactor(plasma-RDR)and a photocatalyst—namely,titanium dioxide(TiO_(2))—were coupled for the treatment of metronidazole(MNZ).Discharge uniformity was improved by the use of a rotating electrode in the plasma-RDR,which contributed to the utilization of ultraviolet(UV)light radiation in the presence of TiO_(2).The experimental results showed that the degradation efficiency of MNZ and the concentration of generated hydroxyl radicals respectively increased by 41%and 2.954 mg∙L^(-1) as the rotational speed increased from 0 to 500 r∙min^(-1).The synergistic effect of plasma-RDR plus TiO_(2) on the generation of hydroxyl radicals was *** intermediate products were identified using three-dimensional(3D)excitation emission fluorescence matrices(EEFMs)and liquid chromatography-mass spectrometry(LC-MS),and a possible degradation pathway is proposed *** plasma-catalytic process has bright prospects in the field of antibiotics degradation.

关键词： Plasma-assisted rotating disk reactor Pulsed discharge Metronidazole Degradation TiO_(2)

Association of CX3CL1 and CX3CR1 Expression with Liver Fibrosis in a Mouse Model of Schistosomiasis

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2020年第6期40卷 1121-1127页

作者： Pan ZHANG bao-ju wang jun-zhong wang Xu-mao XIE Qiao-xiao TONG Department of Emergency Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Infectious Diseases Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Immunopathological mechanisms of schistosomiasis,a debilitating parasitic disease,are still *** this study,we investigated the involvement of CX3C chemokine ligand 1(CX3CL1)and its sole receptor CX3CR1 in the development of liver fibrosis in *** animal model of schistosomiasis was established by infection of C57BL/6 mice with Schistosoma japonicum cercariae;mice injected with carbon tetrachloride(CCl4)were used as positive control of liver *** 4 and 8 weeks,the degree of liver lesions was assessed by hematoxylin and eosin staining,serum levels of hyaluronic acid(HA)were analyzed by a chemiluminescence immunoassay,liver fibrosis was evaluated by immunohistochemistry analysis ofα-smooth muscle actin(α-SMA)expression,and CX3CL1 and CX3CR1 expression in the liver was measured by immunohistochemistry and real-time *** results showed that at 8 weeks after Schistosoma infection,serum HA levels were increased andα-SMA-expressing cells appeared in the liver,indicating ***3CL1-and CX3CR1-positive cells were observed in the outer layer of granulomas formed around Schistosoma eggs in liver tissues,which was consistent with the significant upregulation of hepatic CX3CL1 and CX3CR1 mRNA expression at 4 and 8 weeks ***,correlation analysis revealed positive association between CX3CL1 and CX3CR1 expression and serum HA levels at 8 weeks post-infection,indicating a link between fibrogenesis and the CX3CL1/CX3CR1 axis in *** conclusion,our data suggest the involvement of CX3CL1 and CX3CR1 in the progression of liver fibrosis caused by Schistosoma infection.

关键词： schistosomiasis Schistosoma japonicum liver fibrosis CX3CL1 CX3CR1 hyaluronic acid

Preparation of Pd/c-Al_(2)O_(3)/nickel foam monolithic catalyst and its performance for selective hydrogenation in a rotating packed bed reactor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2022年第1期35卷 311-319页

作者： Hai-Long Liao bao-ju wang Ya-Zhao Liu Yong Luo Jie-Xin wang Guang-Wen Chu Jian-Feng Chen State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Research Center of the Ministry of Education for High Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China

Selective hydrogenation plays an important role in chemical industries,yet its selectivity is usually limited by the mass *** this work,the enhanced hydrogenation selectivity was achieved in a rotating packed bed(RPB)reactor with excellent mass transfer *** to be used under the centrifugal filed,a monolithic catalyst Pd/c-Al_(2)O_(3)/nickel foam suiting for the shape and size of the rotor of RPB reactor was prepared by the electrophoretic deposition *** mechanical strength of the catalyst can meet the requirement of high centrifugal force in the *** hydrogenation selectivity in the RPB reactor using the 3-methyl-1-pentyn-3-ol hydrogenation system was 3–8 times higher than that in a stirred tank reactor under similar *** work proves the feasibility of intensifying the selectivity of hydrogenation process in the RPB reactor.

关键词： Monolithic catalyst Rotating packed bed reactor Hydrogenation Selectivity

Expression of PTEN,PPM1A and P-Smad2 in hepatocellular carcinomas and adjacent liver tissues

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第34期13卷 4554-4559页

作者： Shu-Kun Wu bao-ju wang Yan Yang Xin-Hua Feng Xi-Ping Zhao Dong-Liang Yang Dong-LiangYang Division of Clinical Immunology Tongji Hospitai-Tongji Medical College Huazhong University of Science andTechnology Wuhan 430030 Hubei Province China Center of Experimental MedicineResearch Tongji Hospital Tongji Medical College HuazhongUniversity of Science and Technology Wuhan 430030 HubeiProvince China Department of Molecular and Cellular Biology Baylor College of Medicine Houston TX 77030 United States Department of infectious Disease Hubei ProvinceCorps Hospital The Chinese People's Armed Police ForcesWuhan 430061 Hubei Province China

AIM： To investigate the expressions of PTEN, PPMIA and P-Smad2 in hepatocellular carcinoma （HCC） and their significance.METHODS： The expressions of PTEN, PPMIA and P-Smad2 in 31 HCC tissues, 25 adjacent liver tissues and 13 non-tumor liver tissues were detected by using Envision immunohistochemical technique. RESULTS： The positive expression （64.52%） and staining intensity （4.19 ± 3.31） of PTEN in the cytoplasm of HCC were significantly lower and weaker than those in the adjacent or non-tumor liver tissues （97.37%, 7.88 ± 0.93; 100%, 7.77 ± 0.93, respectively）（P 〈 0.05）, and its staining intensity in the cytoplasm of HCC, which belongs to Edmondson pathologic grades Ⅱ-Ⅲ and above, was also lower than that of grade I and I-Ⅱ. Furthermore, its location in the nucleus or cytoplasm of liver cells was negatively correlated with the progression of liver disease （r = -0.339, P = 0.002）; most of PPMIA might be only expressed in the nucleus of adjacent liver tissues, non-HCC tissues or Edmondson grade I and I - Ⅱ HCC, but it was mainly expressed in the cytoplasm of HCC with Edmondson grade ≥ Ⅱ, weakly or negatively expressed in the nucleus （P 〈 0.05）, and its location was negatively correlated with the progression of liver disease （r = -0.45, P = 0.0000）. P-Smad2, which was mostly located in the nucleus and cytoplasm of grade I and I -Ⅱ HCC, surrounding or non-tumor liver tissues, was only in the nucleus of HCC with Edmondson grade Ⅱ and above （P 〈 0.001）, and its location was positively correlated with the disease progression （r = 0.224, P = 0.016）. Spearman correlation analysis revealed that P-Smad2 was significantly negatively correlated with PTEN and PPMIA （r = -0.748, P = 0.000; r = -0.366, P = 0.001, respectively）; and PTEN and PPMIA were positively correlated with HCC carcinogenesis （r = 0.428, P = 0.000）.CONCLUSION： The aberrant location of expression and staining intensity of PTEN, PPMIA and P-Smad2 in HCC and their relationship might have an impact on the p

关键词： Hepatocellular carcinoma PhosphorylatedSmad2 PTEN PPMIA

Immunostaining of PD-1/PD-Ls in liver tissues of patients with hepatitis and hepatocellular carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第28期17卷 3322-3329页

作者： bao-ju wang jun-Jie bao jun-Zhongwang Yang wang Min Jiang Ming-You Xing Wan-Guang Zhang jun-Ying Qi Michael Roggendorf Meng-Ji Lu Dong-Liang Yang Division of Clinical Immunology TongjiHospital of Tongji Medical College Huazhong University of Sci-ence and Technology Wuhan 430030 Hubei Province China Department of Clinical Laboratory GuangzhouWomen and Children＇s Medical Center Guangzhou 510055Guangdong Province China Department of Microbiology TongjiMedical College Huazhong University of Science and Technol-ogy Wuhan 430015 Hubei Province China Department of Infectious Disease Tongji Hospital of Tongji Medical College Huazhong Universityof Science and Technology Wuhan 430030 Hubei ProvinceChina Hepatic Surgery Center Tongji Hospitalof Tongji Medical College Huazhong University of Science andTechnology Wuhan 430030 Hubei Province China Institute of Virology Univer-sity Hospital Essen Hufelandstrasse 55 45122 Essen Germany

AIM: To investigate the expression of programmed death (PD)-1,PD ligand 1 (PD-L1) and PD-L2 in liver tissues in the context of chronic hepatitis and hepatocellular carcinoma (HCC).METHODS: Liver biopsies and HCC specimens from patients were collected and histologically *** expression of PD-1,PD-L1,and PD-L2 in biopsy specimens of chronic hepatitis and HCC specimens was evaluated by immunohistochemical *** association between the expression level of PD-1,PD-L1,and PD-L2 and clinical and pathological variables was analyzed ***: Expression of PD-1 was found in liverinfiltrating *** contrast,PD-L1 and PD-L2 were expressed in non-parenchyma liver cells and tumor *** expression of PD-L1 was significantly correlated with hepatitis B virus infection (1.42 ± 1.165 vs 0.50 ± 0.756,P = 0.047) and with the stage of HCC (7.50 ± 2.121 vs 1.75 ± 1.500 vs 3.00 ± 0.001,P = 0.018).PD-1 and PD-Ls were significantly up-regulated in HCC specimens (1.40 ± 1.536 vs 5.71 ± 4.051,P = 0.000;1.05 ± 1.099 vs 4.29 ± 3.885,P = 0.004;1.80 ± 1.473 vs 3.81 ± 3.400,P = 0.020).CONCLUSION: PD-L1 may contribute to negative regulation of the immune response in chronic hepatitis ***-1 and PD-Ls may play a role in immune evasion of tumors.

关键词： Hepatitis B virus Programmed death-1 Programmed death ligands Hepatitis Hepatocellular carcinoma

Replication of clinical hepatitis B virus isolate and its application for selecting antiviral agents for chronic hepatitis B patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第22期14卷 3490-3496页

作者： Yin-Ping Lu Tao Guo bao-ju wang Ji-Hua Dong Jian-Fang Zhu Zhao Liu Meng-Ji Lu Dong-Liang Yang Department of Virology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Division of Clinical Immunology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Institute of Virology Duisburg-Essen University Essen 45122 Germany

AIM: To establish a cell model harboring replicative clinical hepatitis B virus (HBV) isolates and evaluate its application in individualized selection of anti-HBV agents for chronic hepatitis B (CHB) patients. METHODS: The full-length HBV genomic DNA from 8 CHB patients was amplified by polymerase chain reaction (PCR). All the patients were treated with lamivudine for at least seven months and finally became resistant to lamivudine. The amplified HBV DNA fragments were inserted into pHY106 vectors by Sap Ⅰ?digestion. The recombinant plasmids containing 1.1 copies of HBV genome were transiently transfected into Huh7 cell line, and the levels of HBsAg, HBeAg and intercellular HBV replicative intermediates were determined by ELISA and Southern blot analysis, respectively, with or without lamivudine and adefovir treatment. The antiviral treatment with adefovir was administered to the patients and analyzed in parallel. RESULTS: A total of 25 independent HBV isolateswere obtained from the sera of 8 patients, each patient had at least two isolates. One isolate from each individual was selected and subcloned into pHY106 vector, including 5 isolates with YVDD mutation and 3 isolates with YIDD mutation. All recombinant plasmids harboring HBV isolates were transfected into Huh7 cells. The results indicated that HBV genome carried in HBV replicons of clinical HBV isolates could effectively replicate and express in Huh7 cells. Adefovir, but not lamivudine, inhibited HBV replication both in vitro and in vivo, and in vitro inhibition was dose-dependent. CONCLUSION: The novel method described herein enables individualized selection of anti-HBV agents in clinic and is useful in future studies of antiviral therapy for CHB.

关键词： Hepatitis B virus Chronic hepatitis B Hepatitis B virus isolate Antiviral agents

Role of iron ore in enhancing gasification of iron coke:Structural evolution,influence mechanism and kinetic analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 58-69页

作者： Jie wang Wei wang Xuheng Chen junfang bao Qiuyue Hao Heng Zheng Runsheng Xu School of Chemistry and Materials Science Hubei Engineering UniversityXiaogan 432000China Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education Wuhan University of Science and TechnologyWuhan 430081China Institue of Ironmaking Technology Baosteel Central Research InstituteShanghai 201999China State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

The utilization of iron coke provides a green pathway for low-carbon *** uncover the influence mechanism of iron ore on the behavior and kinetics of iron coke gasification,the effect of iron ore on the microstructure of iron coke was ***,a comparative study of the gasification reactions between iron coke and coke was conducted through non-isothermal thermogravimetric *** findings indicate that compared to coke,iron coke exhibits an augmentation in micropores and specific surface area,and the micropores further extend and *** provides more adsorption sites for CO_(2) molecules during the gasification process,resulting in a reduction in the initial gasification temperature of iron *** the heating rate in non-isothermal gasification can enhance the reactivity of iron *** metallic iron reduced from iron ore is embedded in the carbon matrix,reducing the orderliness of the carbon structure,which is primarily responsible for the heightened reactivity of the carbon *** kinetic study indicates that the random pore model can effectively represent the gasification process of iron coke due to its rich pore ***,as the proportion of iron ore increases,the activation energy for the carbon gasification gradually decreases,from 246.2 kJ/mol for coke to 192.5 kJ/mol for iron coke 15wt%.

关键词： low-carbon ironmaking iron coke gasification structural evolution kinetic model

Construction and Characterization of a Hepatitis B Virus Replicon

在线全文

学校读者我要写书评

暂无评论

中国病毒学 2007年第1期22卷 8-13页

作者： Yin-ping LU bao-ju wang Ji-hua DONG Zhao LIU Shi-he GUAN Meng-ji LU Dong-liang YANG Department of Virology Union Hospital of Tongji Medical College Huazhong University of Science and Tech- nology Wuhan 430022 P R. China Division of Clinical Immunology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 P. R. China Institute of Virology Duisburg-Essen University Essen 45147 Germany

建立复制细胞肝炎 B 病毒(HBV ) 当模特儿并且在抗病毒的药评估决定它的应用程序，我们构造了表情包含了 HBV 染色体的 1.3 个拷贝，并且在 Huh7 房间在短暂 transfection 以后测量了病毒的复制的水平的 plasmid。我们然后观察了抗病毒... 详细信息

建立复制细胞肝炎 B 病毒(HBV ) 当模特儿并且在抗病毒的药评估决定它的应用程序，我们构造了表情包含了 HBV 染色体的 1.3 个拷贝，并且在 Huh7 房间在短暂 transfection 以后测量了病毒的复制的水平的 plasmid。我们然后观察了抗病毒的药管理的效果。HBV (ayw ) 基因碎片的 1.3 褶层被 PCR 和限制 endonuclease 消化克隆进 pCR2.1。recombinant plasmid 是进 Huh7 房间， HBsAg， HBeAg 和 HBV 的短暂 transfected 在 Huh7 房间的上层清液的 DNA 被 ELISA 和即时 PCR 分别地测量；细胞内部的 HBV replicative 中介和细胞内部的 HBV 抄本被南部的污点和北污点分别地检测。adefovir 的抗病毒的效果，新奇 anti-HBV 核苷酸类似物，在这个细胞的模型系统被评估。结果显示 HBV replicon 的 recombinant plasmid 成功地被构造；在 plasmid pHBV1.3 带的 HBV 染色体能高效地复制并且被表示在哈 7 个房间， adefovir 能在这个细胞的模型，和抑制禁止 HBV 复制是剂量依赖者。结论是 HBV replicon，能在 hepatoma 房间高效地开始病毒的复制，可以是在 HBV 复制和抗病毒的药的学习的一个有用工具。关键词肝炎 B 病毒 - 传染 replicon - 表示向量 CLC 数字 R373 基础条款：国家自然科学基础(No.30271170， No.30170889 ) 。

关键词： Hepatitis B virus Infectious replicon Expression vector

Antiviral Effect of Interferon-Induced Guanylate Binding Protein-1 against Coxsackie Virus and Hepatitis B Virus B3 in Vitro

在线全文

学校读者我要写书评

暂无评论

中国病毒学 2007年第3期22卷 193-198页

作者： Yin-ping LU　 bao-ju wang　 Ji-hua DONG　 Zhao LIU　 Shi-he GUAN　 Meng-ji LU 　 Dong-liang YANG Department of Virology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Division of Clinical Immunology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Institute of Virology Duisburg-Essen University Essen 45147 Germany

Guanylate binding protein-1(GBP-1)is an interferon-induced *** observe its antiviral effect against Hepatitis B virus(HBV)and Coxsackie virus B3(CVB3),we constructed an eukaryotic expression vector of human GBP-1(hGBP-1).Full-length encoding sequence of hGBP-1 was amplified by long chain RT-PCR and inserted into a pCR2.1 vector,then subcloned into a pCDNA3.1(-)*** hGBP-1 plasmids and pHBV1.3 carrying 1.3-fold genome of HBV were contransfected into HepG2 cells,and inhibition effect of hGBP-1 against HBV replication was *** cells transfected with recombinant hGBP-1 plasmids were challenged with CVB3,and viral yield in cultures were *** results indicated that recombinant eukaryotic expression plasmid of hGBP-1 was constructed successfully and the hGBP-1 gene carried in this plasmid could be efficiently expressed in HepG2 cells and Hela ***-1 inhibit CVB3 but not HBV replication in *** results demonstrate that hGBP-1 mediates an antiviral effect against CVB3 but not HBV and perhaps plays an important role in the interferon-mediated antiviral response against CVB3.

关键词：柯萨奇病毒干扰素乙型肝炎病毒抗滤过性病原体鸟苷酸结合蛋白质