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Genetic variability of Aspergillus terreus from dried grapes

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Advances in Bioscience and Biotechnology 2010年第4期1卷 345-353页

作者： banu narasimhan Madhivathani Asokan 不详

RAPD was used to examine the genetic variability among five isolates of Aspergillus terreus spp.. Two random primers were selected for the RAPD assay PG01–5’ CAGGTGTTGC 3’ and PG02–5’ CTGGACAGAC 3’ (Progen Technologies). The characterization of Aspergillus terreus species have been mostly applied on the basis of morphology, phenotype and physiology. DNA Polymorphisms are based on differences in DNA sequences and have advantages over protein polymorphisms. But morphological characterization besides molecular tools will remain a basic and powerful key in the identification of Aspergillus terreus species. The objective of the present study was to isolate the fungal contaminants from dried grapes and compare the genomic profile of the Aspergillus terreus speices isolated from the dried grapes, through RAPD analysis. In the present study with primer PG 01 four different discriminations was there among the A. terreus isolates. There was a homology of genotype between the isolates 1 & 3. And with primer PG 02 four different discriminations were there and there was a homology between 1 & 3. The predominant type was type I in primer I & II. The other isolates belonged to 2, 3 and 4. No similarity was detected for isolates 3, 4 and 5 indicating great genomic diversity of A. terreus.

关键词： Aspergillus Terreus Dried Grapes Genetic Variability RAPD-PCR.

DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis

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Advances in Bioscience and Biotechnology 2010年第2期1卷 136-144页

作者： Swarnakaran Hemalatha narasimhan banu 不详

Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. In the present study, antibiotic resistance, heavy metal tolerance &molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. Bacillus cereus resistant to Penicillin (10 units/ml) and Amoxycillin (10 µg/ml);sensitive to Cobalt and Mercury even at 1 mM concentration were determined. All the isolate had plasmid ranging in number from 3-5 and their molecular weight ranged from 3.0 kb-2.0 kb. Plasmid DNA band specific to 6,557 bp has been found in Bacillus cereus isolated from sewage water, air, fresh water, sea water and milk.

关键词： Bacillus Cereus Antibiotic Resistance Heavy Metal Tolerance RFLP DNA Finger Printing

Enumeration of microbial contaminants in sachet water: a public health challenge

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Health 2010年第6期2卷 582-588页

作者： narasimhan banu Himabindu Menakuru 不详

Accessibility and availability of fresh clean water is a key to sustainable development and essential element in health, food production and poverty reduction. In the present study, we have collected water sachet containing CM/L number and they were analysed for physical and bacteriological nature. The organisms isolated in this study were Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas vesicularis and Pseudomonas aeruginosae. The harmful effects of these isolates were evidenced by antibiotic resistance, heavy metal tolerance and antibacterial activity. They were resistant to the antibiotics like amoxiclav, methicillin, chloramphenicol and streptomycin. They showed tolerance to the heavy metals at 5 mM conc. except for lead. For antibacterial activity, they were tested against human pathogens Klebsiella pnemoniae, Proteus mirabilis, Micrococcus leuteus and Salmonella paratyphium. But at the same time these organisms could be exploited for the industrial production of amylase, protease and cellulase.

关键词： Sachet Water Bacterial Contaminants Pathogens Industrially Useful Bacteria

Aflatoxin B1 contamination in sunflower oil collected from sunflower oil refinery situated in Karnataka

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Health 2010年第8期2卷 973-987页

作者： narasimhan banu JohnPaul Muthumary 不详

In the present study, the aflatoxin B1 contamination at various stages of oil refining and in refined oil were carried out. This was subsequently compared with commercial vegetable oil samples. Among the 23 different sunflower oil samples were tested, 10 of them showed posi-tive results to AFB1 and the remaining 13 showed negative results to AFB1. All the refined oil samples were free from AFB1 contamination.

关键词： Aflatoxin B1 Refined Oil Raw Oil Filtered Oil Sunflower Oil Edible Oil

A Study on Biosorption Potential of <i>Aspergillus</i>sp. of Tannery Effluent

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Advances in Bioscience and Biotechnology 2014年第10期5卷 853-860页

作者： Arasappan Sugasini Kalyanaraman Rajagopal narasimhan banu Department of Biotechnology Vels University Chennai India

Chromium toxicity is one of the major environmental pollutants. As conventional methods for heavy metal removal are very expensive, bioremediation using fungi is extensively explored. Therefore in the present investigation, out of 23 fungi isolated from tannery effluent, 4 Aspergillus species namely A. terreus, A. tamarii, A. flavus and A. niger were selected for evaluating chromium tolerance and biosorption potential. Growth of the fungi was tested using various concentrations of Potassium dichromate (Cr VI) viz., 1 mM, 3 mM and 5 mM amended in potato dextrose agar. For all the fungi, growth was observed only in 1 mM and 3 mM. No growth was observed in 5 mM. Biosorption efficiency of both live and alkali pretreated Aspergillus species was compared. In the present study, alkali pretreated form exhibited highest biosorption efficiency than the live biomass in which A. terreus showed highest chromium biosorption potential compared to the other fungi.

关键词： Aspergillus Chromium Alkali Pretreated Biosorption Live Biomass