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Effect of group size and regrouping on physiological stress and behavior of dairy calves

Journal of Integrative Agriculture 2023年第3期22卷 844-852页

作者： LYU Jing WANG Chao ZHAO Xun-wu MIAO Er-yu WANG Zhi-peng XU Yuan bai xiu-juan BAO Jun College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030P.R.China College of Wildlife and Protected Area Northeast Forestry UniversityHarbin 150040P.R.China

Mixing or regrouping of calves from different pens is a common animal management practice on the farm, which frequently occurs after weaning and has a negative effect on calve welfare. Social integration before regrouping may relieve stresses, but more evidences are needed to verify this hypothesis. The present study aimed to investigate acute physiological and behavioral variations of individually-or group-housed calves after being introduced into a mixed group. A total of 132 postnatal calves were randomly divided into groups of 1, 3, 6 and 12 animals(S, G3, G6, and G12;6 replicates in each group) until 59 days of age. At 60 days of age, every two replicates from different groups(S, G3, G6 and G12)were introduced in a larger pen which containing 44 of the aboved experimental calves. Before and after regrouping,physiological parameters of stress, including heart rate(HR), saliva cortisol(S-CORT), saliva secretory immunoglobulin A(SIgA), interleukin-2(IL-2), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) levels, and behavioral responses were recorded. After regrouping, HR and S-CORT increased immediately(P<0.05), and higher(P<0.05) levels of such molecules were found in S calves compared to those in group-housed calves. Levels of SIgA and IL-2 were decreased(P<0.05), and the lowest(P<0.05) IL-2 values were found in S calves compared to those in group-housed calves. In addition, the introduced calves displayed a distinct behavior, including altered active and rest time, which was associated with negative emotions triggered by the novel surroundings. Allogrooming, play, exploration behaviors and lying time were increased significantly(P<0.05) in group-housed calves than those in S calves. Conversely, self-grooming, aggressive behaviors, standing and walking time were increased(P<0.05) in S calves than those in group-housed calves. These findings suggest that individually-housed calves may be more susceptible to stressors arising from regrouping than grouphoused c

关键词： calf regrouping group size behavior stress welfare

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Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions

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Journal of Forestry Research 2011年第1期22卷 123-126页

作者： LIU Zong-yue NING Fang-yong YANG Hong-yan ~ WEI Lai bai xiu-juan Animal science and technology Institute of Northeast Agriculture University Harbin China.

Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis （BSA） in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into sequence-characterized amplified regions （SCAR） marker for rapid detection of this disease. A single RAPD marker A8 amplified a specific band of 263bp in self-biting minks, which was designated as SRA8-250, and non-specific band of 315bp in both self-biting and healthy minks. The sequences of the bands exhibited 75% and 88% similarity to Canis familiarizes major histocompatibility complex （MHC） class II region and Macaca mulatta MHC class I region, respectively. A SCAR marker SCAR-A8 was designed for the specific fragment SRA8-250 and validated in 30 self-biting minks and 30 healthy minks. Positive amplification of SCAR-A8 was detected in 24 self-biting minks and 12 healthy minks. χ2 test showed significant difference （p〈0.01） in the detection rate between the two groups. This indicated that SRA8-250 can be used as a positive marker to detect self-biting disease in minks. Furthermore, the finding that self-biting disease links with MHC genes has significant implications for the mechanism of the disease.

关键词： mink random amplified polymorphic DNA self-biting sequence characterized amplified region

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Effects of Different Feeding Methods on Behaviors, Immunities and Growth Performances of Suckling Calves

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Journal of Northeast Agricultural University(English Edition) 2020年第1期27卷 40-48页

作者： bai xiu-juan Su Hang Bao Jun Lv Jing College of Animal Sciences and Technology Northeast Agricultural UniversityHarbin 150030China

Behaviors,immunities and growth performances of suckling calves to either fed milk through barrel feeding or artificial nipple feeding were investigated in this ***-two newborn female suckling Holstein calves were selected and divided into two treatments which fed regular milk through milk barrels or artificial *** behaviors′parameters were measured on 14,29 and 44 *** weight and body size of the calves were recorded on 15,30 and 45 *** indicators were collected in quiet situation on 15,30 and 45 *** with the barrel feeding method,calves fed by artificial nipples had less non-nutritive behaviors,stronger growth performances and higher immune *** nipples gave calves sucking conditions and reduced the motivation of calves to suck each other,which might promote the development of complex stomachs,allow food to be better digested and more *** also reduced cross-infection indirectly which increased the calves'immunities.

关键词： growth performance behavior immunity artificial nipple barrel

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CⅡTA基因编码区不同单倍型cDNA的功能研究

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中华微生物学和免疫学杂志 2009年第6期29卷 544-547页

作者：柏秀娟张绪清中国人民解放军总医院南楼神经内科北京100853 第三军医大学西南医院全军感染病研究所重庆400038

目的研究MHCⅡ类反式激活因子（CⅡTA）基因编码区非同义单核苷酸多态性（SNP）位点C19170G（Leu45Val）和C30799G（Ala500Gly）构成的4种不同单倍型eDNA的功能。方法将4种不同单倍型的真核表达载体和空载体分别转染至HeLa细胞。用RT—... 详细信息

目的研究MHCⅡ类反式激活因子（CⅡTA）基因编码区非同义单核苷酸多态性（SNP）位点C19170G（Leu45Val）和C30799G（Ala500Gly）构成的4种不同单倍型eDNA的功能。方法将4种不同单倍型的真核表达载体和空载体分别转染至HeLa细胞。用RT—PCR和间接细胞免疫荧光技术检测未经转染的HeLa细胞、转染4种真核表达载体及空载体的HeLa细胞C1ITAmRNA与3种HLAⅡ类分子（HLA-DR、DP、DQ）的表达，并用流式细胞技术对其表达的3种HLAⅡ类蛋白进行定量分析。结果未经转染和转染空载体的HeLa细胞均无CⅡTA mRNA和3种HLAⅡ类分子的表达，而转染4种不同单倍型真核表达载体的HeLa细胞均出现CⅡTA mRNA表达，并表达3种HLAⅡ类分子。证实了转染4种不同单倍型真核表达载体后的HeLa细胞3种HLAU类分子表达水平差异无统计学意义（P均〉0．05）。结论中国人CⅡTA基因编码区这两个SNP位点的多态性（2个位点氨基酸的改变）不影响CⅡTA反式激活HLAⅡ类基因表达的能力。

关键词： MHCⅡ类反式激活因子单核苷酸多态性单倍型 HLAⅡ类分子

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提高阳极一级品率的措施

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轻金属 2010年第8期 50-52,61页

作者：白秀娟中国铝业股份公司青海分公司青海大通810108

通过分析指出影响预焙阳极一级品率的主要因素,结合生产实践提出了现阶段提高预焙阳极一级品率的各项措施。

关键词：预焙阳极品级率灰分理化性能

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Genetic Diversity Analysis Among Populations of Mink from China by Inter-simple Sequence Repeat(ISSR) Markers

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Journal of Northeast Agricultural University(English Edition) 2013年第3期20卷 47-54页

作者： Yang Hong-yan Fu Jing Ning Fang-yong Du Zhi-heng bai xiu-juan College of Animal Sciences and Technology Northeast Agricultural University

Inter-simple Sequence Repeat （ISSR） analysis was applied to assess the genetic diversity within and among five populations of mink from Liaoning Province. A total of 20 primers were screened, five selected primers produced 35 discernible bands, with 30 （85.71%） being polymorphic, indicating high genetic diversity at the species level. The highest genetic diversity was observed in the brown mink population, whereas the lowest diversity was found in the standard-pitchy mink population. Based on genetic distance （1972）, a dendrogram was constructed by using UPGMA algorithm, and five populations were divided into two major groups. Group I consisted of only the standard-pitchy mink population, and Group II included other four populations, in Group II, sapphire mink was close to brown mink population. The results of genetic differentiation indicated that the genetic differentiation degree between populations was lower and the genetic variation primarily came from within populations. This paper showed that ISSR technique was a reliable tool that could be used to study genetic diversity in the mink.

关键词： mink ISSR genetic diversity

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美洲黑貂5-HT_(1A)受体基因多态性与自咬行为的相关性

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动物学杂志 2008年第6期43卷 51-56页

作者：林宁白秀娟东北农业大学生命科学学院哈尔滨150030 东北农业大学动物科技学院哈尔滨150030

以美洲黑貂(Mustela vison)5-羟色胺1A(5-HT1A)受体基因作为影响其自咬行为的可能候选基因,分析该基因对美洲黑貂自咬行为的影响。本实验采用单链构象多态性(PCR-SSCP)方法进行了多态性检测,并对该基因的不同基因型与自咬行为的关系进... 详细信息

以美洲黑貂(Mustela vison)5-羟色胺1A(5-HT1A)受体基因作为影响其自咬行为的可能候选基因,分析该基因对美洲黑貂自咬行为的影响。本实验采用单链构象多态性(PCR-SSCP)方法进行了多态性检测,并对该基因的不同基因型与自咬行为的关系进行分析。结果表明,在美洲黑貂群体中5-HT1A受体基因136位点发生碱基突变(T-G)的SNPs对美洲黑貂自咬行为性状无影响(χ2=1.3936,P>0.2),在287位点发生(C-G)突变对美洲黑貂的自咬行为有一定的影响(χ2=3.7694,P<0.2)。

关键词：美洲黑貂 5-HT1A 受体基因自咬行为 PCR—SSCP

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多喷嘴射水式喷射器加热性能分析

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重庆大学学报（自然科学版） 2011年第6期34卷 60-63页

作者：童明伟蔡琴白秀娟重庆大学动力工程学院重庆400044

为研究多喷嘴射水式喷射器的加热性能,提出了分析喷射器加热系数的计算模型,对喷射器加热性能的影响因素进行了分析,并根据理论分析和实验数据得出了不同运行参数对其加热性能的影响规律。研究结果表明,多喷嘴射水式喷射器具有很好的加... 详细信息

为研究多喷嘴射水式喷射器的加热性能,提出了分析喷射器加热系数的计算模型,对喷射器加热性能的影响因素进行了分析,并根据理论分析和实验数据得出了不同运行参数对其加热性能的影响规律。研究结果表明,多喷嘴射水式喷射器具有很好的加热性能,喷射器的加热系数随入口水温的升高而减小,随蒸汽压力和引射系数的升高而增大,并且计算和实验得到的各运行参数对喷射器加热性能的影响规律是基本一致的。

关键词：喷射器多喷嘴加热系数引射系数

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梅花鹿生长激素基因单核苷酸多态与产茸量性状的相关性

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遗传 2007年第3期29卷 337-342页

作者：杜智恒白秀娟东北农业大学动物科技学院哈尔滨150030

以梅花鹿的生长激素基因(GH)作为候选基因,分析该基因对梅花鹿产茸量性状的影响。以吉林农业大学鹿场提供的梅花鹿为实验群体,采用单链构象多态性(PCR-SSCP)和DNA测序的方法检测了GH基因单核苷酸多态性(SNPs),针对该群体的特点建立合适... 详细信息

以梅花鹿的生长激素基因(GH)作为候选基因,分析该基因对梅花鹿产茸量性状的影响。以吉林农业大学鹿场提供的梅花鹿为实验群体,采用单链构象多态性(PCR-SSCP)和DNA测序的方法检测了GH基因单核苷酸多态性(SNPs),针对该群体的特点建立合适的统计分析模型,并进行了GH基因多态性与产茸量的相关分析。结果表明,GH基因对梅花鹿的产茸量有一定影响。G→A突变产生的3种基因型间的第五锯产茸量存在一定的差异(P<0.2),BB基因型个体在第五锯的产茸量与AA基因型个体之间有一定的差异(P<0.2)。

关键词：梅花鹿 GH基因单核苷酸多态产茸量性状

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Masking quantum information in multipartite systems via Fourier and Hadamard matrices

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Communications in Theoretical Physics 2025年第2期77卷 101-111页

作者： Chen-Ming bai Meng-Ya Wang Su-juan Zhang Lu Liu Department of Mathematics and Physics Shijiazhuang Tiedao University

Quantum information masking（QIM） is a crucial technique for protecting quantum data from being accessed by local subsystems. In this paper, we introduce a novel method for achieving1-uniform QIM in multipartite systems utilizing a Fourier matrix. We further extend this approach to construct an orthogonal array with the aid of a Hadamard matrix, which is a specific type of Fourier matrix. This allows us to explore the relationship between 2-uniform QIM and orthogonal arrays. Through this framework, we derive two distinct 2-uniform quantum states,enabling the 2-uniform masking of original information within multipartite ***, we prove that the maximum number of quantum bits required for achieving a2-uniformly masked state is 2~n-1, and the minimum is 2n-1+ 3. Moreover, our scheme effectively demonstrates the rich quantum correlations between multipartite systems and has potential application value in quantum secret sharing.

关键词： multipartite systems quantum information masking Fourier matrix orthogonal arrays

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