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Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue

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Science China Chemistry 2012年第8期55卷 1657-1662页

作者： bae sang-woo CHANG woo-Jin KOO Yoon-Mo HA Sung Ho Department of Biological Engineering Inha University Incheon 402-751 Republic of Korea Department of Mechanical Engineering School of Freshwater Sciences University of Wisconsin-Milwaukee Milwaukee WI 53201-0413 USA Department of Chemical Engineering and Nano-Bio Technology Hannam University Daejeon 305-811 Republic of Korea

The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies （IBs）. Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids （RTILs） on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme re- folding yield was obtained in l-ethyl-3-methylimidazolium tetrafluoroborate （[EMIM][BF4]） containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate （[BMIM][MS]） containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intrarnolecular disulfide bonds. For dilution-based refolding of lysozyme, the opti- mum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [PMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 ℃.

关键词： protein refolding room temperature ionic liquid dilution refolding lysozyme hydrophobicity sulfur

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Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels,Orai1 and TRPV3

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Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 580-585页

作者： Joo Hyun Nam Hyo Won Jung Young-Won Chin woo Kyung Kim Hyo sang bae Department of Physiology College of MedicineChannelopathy Research Center(CRC)Dongguk University Department of Herbology College of Korean MedicineDongguk University College of Pharmacy Dongguk University Department of Internal Medicine Graduate School of MedicineChannelopathy Research Center(CRC)Dongguk University Department of Sasang Constitutional Medicine College of Korean MedicineDongguk University

Objective: To examine the effects of Tribulus terrestris L.(T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic ***: The 70% methanol extract of T. terrestris was prepared. Human HEK293 T cells with over-expressed calcium release-activated calcium channel protein 1(Orai1),transient receptor potential vanilloid 1, or transient receptor potential vanilloid 3(TRPV3)were treated with T. terrestris extract. Modulation of ion channels was measured using a conventional whole-cell patch-clamp ***: T. terrestris extract(100 mg/m L) significantly inhibited Orai1 activity in Orai1-stromal interaction molecule 1 co-overexpressed HEK293 T cells. In addition, T. terrestris extract significantly increased the TRPV3 activity compared with 2-Aminoethyl diphenylborinate(100 mmol/L), which induces the full activation of ***: Our results suggest that T. terrestris extract may have a therapeutic potential for recovery of abnormal skin barrier pathologies in atopic dermatitis through modulating the activities of calcium ion channels, Orai1 and TRPV3. This is the first study to report the modulatory effect of a medicinal plant on the function of ion channels in skin barrier.

关键词： Tribulus terrestris L. Atopic dermatitis Ion channels ORAI1 TRPV3

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Clinical implications and perspectives of portal venous circulating tumor cells in pancreatic cancer

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World Journal of Gastrointestinal Oncology 2023年第4期15卷 632-643页

作者： Sung woo Ko Seung bae Yoon Department of Internal Medicine College of MedicineThe Catholic University of KoreaSeoul 06591South Korea Department of Internal Medicine Eunpyeong St.Mary’s HospitalSeoul 03312South Korea

Despite recent improvements in the diagnosis and treatment of pancreatic cancer(PC),clinical outcomes remain ***,there are no effective prognostic or predictive biomarkers or options beyond carbohydrate antigen 19-9 for personalized and precise *** tumor cells(CTCs),as a member of the liquid biopsy family,could be a promising biomarker;however,the rarity of CTCs in peripheral venous blood limits their clinical *** the first venous drainage of PC is portal circulation,the portal vein can be a more suitable location for the detection of *** ultrasound-guided portal venous sampling of CTCs is both feasible and *** studies have suggested that the detection rate and number of CTCs may be higher in the portal blood than in the peripheral *** counts in the portal blood are highly associated with hepatic metastasis,recurrence after surgery,and *** phenotypic and genotypic properties measured in the captured portal CTCs can help us to understand tumor heterogeneity and predict the prognosis of *** sample sizes and heterogeneous CTC detection methods limit the studies to ***,a large number of prospective studies are needed to corroborate portal CTCs as a valid biomarker in PC.

关键词： Circulating tumor cell Pancreatic cancer Portal vein Outcomes Prognosis Survival

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Stress-induced cardiomyopathy with electrocardiographic ST-segment elevation in a patient with pneumothorax

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World Journal of Emergency Medicine 2020年第4期11卷 260-262页

作者： Jun woo Cho Chi Hoon bae Department of Thoracic and Cardiovascular Surgery School of MedicineCatholic University of DaeguDaeguRepublic of Korea

Dear editor,In many cases,S T-segment changes on electrocardiogram(ECG)are suggestive of acute coronary syndrome,but there may be other causes,[12]such as stress induced ***,reports of such changes in patients with pneumothorax are very rare.[3-1]Here,we report a case of ST-segment elevation due to stress-induced cardiomyopathy in a patient with pneumothorax.

关键词： cardiomyopathy acute patient

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Estimation of carbon storage based on individual tree detection in Pinus densiflora stands using a fusion of aerial photography and LiDAR data

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Science China(Life Sciences) 2010年第7期53卷 885-897页

作者： KIM So-Ra KWAK Doo-Ahn LEE woo-Kyun SON Yowhan bae sang-Won KIM Choonsig YOO Seongjin Division of Environmental Science and Ecological Engineering Korea University Forest Practice Research Center Korea Forest Research Institute Departement of Forest Resources Jinju National University

The objective of this study was to estimate the carbon storage capacity of Pinus densiflora stands using remotely sensed data by combining digital aerial photography with light detection and ranging(LiDAR) data.A digital canopy model(DCM),generated from the LiDAR data,was combined with aerial photography for segmenting crowns of individual *** eliminate errors in over and under-segmentation,the combined image was smoothed using a Gaussian filtering *** processed image was then segmented into individual trees using a marker-controlled watershed segmentation *** measuring the crown area from the segmented individual trees,the individual tree diameter at breast height(DBH) was estimated using a regression function developed from the relationship observed between the field-measured DBH and crown *** above ground biomass of individual trees could be calculated by an image-derived DBH using a regression function developed by the Korea Forest Research *** carbon storage,based on individual trees,was estimated by simple multiplication using the carbon conversion index(0.5),as suggested in guidelines from the Intergovernmental Panel on Climate *** mean carbon storage per individual tree was estimated and then compared with the field-measured *** study suggested that the biomass and carbon storage in a large forest area can be effectively estimated using aerial photographs and LiDAR data.

关键词： LiDAR aerial photograph region growing marker-control watershed segmentation carbon storage

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Carbon and nitrogen dynamics in a Pinus densiflora forest with low and high stand densities

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Journal of Plant Ecology 2013年第5期6卷 368-379页

作者： Nam Jin Noh Choonsig Kim sang Won bae woo Kyun Lee Tae Kyung Yoon Hiroyuki Muraoka Yowhan Son Division of Environmental Science and Ecological Engineering Korea UniversitySeoul 136-713Republic of Korea River Basin Research Center Gifu UniversityGifu 501-1193Japan Department of Forest Resources Gyeongnam National University of Science and TechnologyJinju 660-758Republic of Korea Department of Forest Conservation Korea Forest Research InstituteSeoul 130-712Republic of Korea

Aims Understanding carbon(C)and nitrogen(N)dynamics and their dependence on the stand density of an even-aged,mature forest provides knowledge that is important for forest *** study investigated the differences in ecosystem total C and N storage and flux between a low-density stand(LD)and a high-density stand(HD)and examined the effects of stand density on aboveground net primary productivity(ANPP),total belowground C allocation(TBCA)and net ecosystem production(NEP)in a naturally regenerated,65-to 75-year-old Pinus densiflora *** *** LD(450 trees ha^(−1))and HD(842 trees ha^(−1))were established in an even-aged,mature *** forest in September *** forest had been naturally regenerated following harvesting,and the stand density was naturally maintained without any artificial management such as *** diameter at breast height(DBH≥5.0cm)of all live stems within the stands was measured yearly from 2007 to *** compare C and N storage and fluxes in LD and HD,C and N pools in aboveground and belowground biomass,the forest floor,coarse woody debris(CWD)and soil;soil CO_(2) efflux(R_(S));autotrophic respiration(R_(A));litter production;and soil N availability were ***,ANPP,TBCA and NEP were estimated from plot-based measurement *** Findings Ecosystem C(Mg C ha^(−1))and N(Mg N ha^(−1))storage was,respectively,173.0±7.3(mean±SE)and 4.69±0.30 for LD and 162±11.8 and 4.08±0.18 for *** were no significant differences in C and N storage in the ecosystem components,except for soils,between the two *** contrast,there were significant differences in aboveground ANPP and TBCA between the two stands(P<0.05).Litterfall,biomass increment and R_(S) were major C flux components with values of,respectively,3.89,3.74 and 9.07 Mg C ha^(−1) year^(−1) in LD and 3.15,2.94 and 7.06 Mg C ha^(−1) year^(−1) in ***-based NEP(Mg C ha^(−1) year^(−1))was 4.18 in LD and 5.50 in *** the even-aged,mature P

关键词： aboveground net primary productivity net ecosystem production Japanese red pine stand density total belowground carbon allocation

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SUSTAINABLE VALUE ON CONSTRUCTION PROJECTS AND LEAN CONSTRUCTION

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Journal of Green Building 2008年第1期3卷 156-167页

作者： Jin-woo bae Yong-woo Kim Dept.of Civil and Environmental Engineering College of Engineering130e Architecture HallUniversity of WashingtonSeattleWA 98195 Dept.of Construction Management College of Architecture and Urban Planning120h Architecture HallUniversity of WashingtonSeattleWa 98195

Lean production is a systemic approach to meeting customer expectations, whatever they value, by reducing *** fi rst glance, lean then could only contribute to sustainability, while sustainability is achieved only if the customervalues sustainability. The paper has two objectives. One is to examine whether sustainability is feasible using leanproduction with sustainability as an added value. The second objective is to examine how current lean constructiontools and methods impact the construction and operation of sustainable facilities. The authors also suggest how theselean construction tools and methods have evolved to contribute to green construction. For this study, the authorsinterviewed lean adopters and conducted literature surveys on lean principles and methods, and its impacts on theeconomic, social and environmental sustainability.

关键词： lean construction sustainable construction sustainable assessment sustainability

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Systematic Revision of Trilobites from the Middle Ordovician(Darriwilian) Klimoli Formation of the Zhuozishan Area,Inner Mongolia,China

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Acta Geologica Sinica(English Edition) 2016年第6期90卷 1955-1975页

作者： LEE Seung-bae LEE Dong-Chan woo Jusun ZHANG Xingliang Geology Division Korea Institute of Geoscience and Mineral Resources Department of Earth Science Education Chungbuk National University Division of Polar Earth-System Sciences Korea Polar Research Institute Early Life Institute and State Key Laboratory of Continental Dynamics Department of GeologyNorthwest University

New morphologic information permits systematic revision of trilobites from the Middle Ordovician Klimoli Formation of the Zhuozishan area, Ordos Basin, Inner Mongolia. The new assemblage is composed of 10 species of the Raphiophoridae, Nileidae, Asaphidae, and Telephinidae.？ An asaphid, aff. Mioptychopyge lashachungensis （previously Paraptychopyge lashachungensis） displays an intermediate morphology between the Chinese nobiliasaphine genera Mioptychopyge and Zhenganites. The pygidial doublure is regarded as the most significant character to differentiate Symphysurus klimoliensis （previously Nileus klimoliensis） of the Nileidae from such closely allied taxa as Poronileus. A nileid, cf. Peraspis kujandensis displays typical nileid morphology, unlike the type species, Peraspis lineolata, which might turn out to be an asaphid. Ampyx gongwusuensis sp. nov. of the Raphiophoridae is the first record of the genus in the Zhuozishan area and reveals morphologic details that might be employed to resolve Ampyx taxonomy in China. Morphologic differences between A. gongwusuensis and Abulbaspis ordosensis might represent a case of sexual dimorphism.

关键词： Invertebrate paleontology Trilobita systematics Klimoli Formation Darriwilian Zhuozishan Inner Mongolia

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Effects of upper lid blepharoplasty on visual quality in patients with lash ptosis and dermatochalasis

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International Journal of Ophthalmology(English edition) 2016年第9期9卷 1320-1324页

作者： Scoung Hyun An sang wook Jin Yoon Hyung Kwon Won Yeol Ryu woo Jin Jeong Hee bae Ahn Department of Ophthalmology Dong-A University College of Medicine Busan 602-715 Korea

AIM：To assess the effects of upper lid blepharoplasty on visual ***：Seventy-three eyelids of 39 patients were subjected to upper lid ***- and postoperative contrast sensitivity,visual acuity,vertical palpebral aperture and the degree of lash ptosis were ***：The mean log contrast sensitivities under photopic（P =0.017） and scotopic conditions（P =0.009）were improved after surgery,and these differences were *** degree of lash ptosis was also decreased after blepharoplasty（P〈0.001）.CONCLUSION：In our study,a significant increase in contrast sensitivity was found after *** results suggest that upper lid blepharoplasty can be helpful for improving visual quality.

关键词： blepharoplasty visual quality contrast sensitivity

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Dendritic cell-based immunotherapy for colon cancer using an HLA-A＊0201-restricted cytotoxic T-lymphocyte epitope from tumor-associated antigen 90K

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Cellular & Molecular Immunology 2013年第3期10卷 275-282页

作者： Ji Hee Lee Myong-Suk Park Jun-Eul Hwang sang-Hee Cho woo-Kyun bae Hyun-Jeong Shim Dae-Eun Kim Ik-Joo Chung Department of Hematology-Oncology Chonnam National University Medical School Gwangju Korea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National University Gwangiu Korea

Tumor-associated antigen 90K is implicated in cell-cell and cell-extracellular matrix adhesion through its interaction with galectin-3 and integrin-β 1 and is highly expressed in malignant tissues, making it a novel target for the development of new immunotherapies. We investigated a potential immunotherapy treatment for colon cancer using 90K-specific cytotoxic T lymphocytes induced by autologous dendritic cells and pulsed with 90K peptides. We selected three peptides （90K3s1, 90K5 and 90K523） that bind to HLA-A＊0201 molecules on the basis of their binding affinity, as determined by a peptide-T2 binding assay. Dendritic cells pulsed with 90K peptides resulted in the efficient generation of mature dendritic cells and exhibited enhanced T-cell stimulation and polarization of naive T cells toward Thl. Dendritic cells pulsed with 90K peptides generated potent cytotoxic T-lymphocytes that lysed T2 cells loaded with each 90K peptide, and 90K＋/HLA-A2＋ colon cancer cell lines, including HCT116 and SW480, in a dose-dependent and HLA-A＊O2Ol-restricted manner. No killing was observed in 90K＋/HLA-A2- DLD1 or 90K-/HLA-A2- K562 cells. Therefore, we believe that cytotoxic T-lymphocytes stimulated by 90K peptide-pulsed dendritic cells naturally recognize the 90K peptide presented by colon cancer cells in the context of HLA-A2, and kill 90K-positive tumor cells. Dendritic cells pulsed with 90K peptides led to the induction of granzyme B and perforin positive CD8＋ T cells against HCT116 and SW480 cells, but not DLD1 cells. In conclusion, 90K-specific cytotoxic T lymphocytes, generated by stimulating T cells with 90K peptide-pulsed dendritic cells, could be useful effector cells for the immunotherapv treatment of colon cancer.

关键词： 9OK colon cancer dendritic cells immunotherapy peptide

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