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Internal phosphate starvation signaling and external phosphate availability have no obvious effect on the accumulation of cadmium in rice

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Journal of Integrative Agriculture 2019年第9期18卷 2153-2161页

作者： WANG xue-qing RUAN Wen-yuan YI Ke-ke Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences

Phosphorus(P)is a limiting nutrient element for *** obtain maximum crop yield,P fertilizer is often over-applied,which leads to accelerating exhaustion of phosphate resources and serious environmental *** the application of P fertilizer and enhancing the P utilization efficiency of crops are significant for the sustainable development of ***(Cd)contamination in rice is another serious agricultural ***,whether reducing the application of P fertilizer and enhancing the P utilization efficiency of crops will increase the risk of Cd accumulation in crops remains *** this study,we are aiming to elucidate the relationship between Cd and P in rice from physiological and genetic *** this purpose,the wild type(WT)rice plants and phosphate(Pi)-starvation signaling repressed mutant phr2 were used to analyze the relationship between Cd and ***,we found that Cd stress could promote P accumulation and induce Pi-starvation signaling in WT and phr2 shoots under Pi-sufficient condition in a PHOSPHATE STARVATION RESPONSE 2(PHR2)independent ***,the expression level of Cd transporter of OsNramp5 and the uptake speed of Cd2+were not obviously changed under Pi-sufficient and Pi-deficient ***,our Cd determination results showed that the Cd concentrations in WT and phr2 were not obviously changed under Pi-sufficient and Pi-deficient *** results indicate that the external P availability and internal Pi-starvation signaling cannot obviously affect the accumulation of Cd in rice seedling.

关键词： cadmium phosphorus Cd-accumulation Pi-starvation signaling Pi-availability

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Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第6期12卷 436-447页

作者： Yuan-fei ZHOU Xian-yin ZHANG qing-zhong xue Department of Agronomy College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Pei＇ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile （PTGMS） genes, has been widely exploited for commercial seed production for ＂two-line＂ hybrid rice in China. One PTGMS gene from Pei＇ai64S, pmsl（t）, was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat （SSR） and insert and deletion （In-Del） markers. Using linkage analysis for the F2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei＇ai64S and 93-11 （indica restorer） lines, the pmsl（t） gene was delimited to the region between the RM21242 （0.2 cM） and YF11 （0.2 cM） markers on the short arm of chromosome 7. The interval containing the pmsl（t） locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research （TIGR） Genomic Annotation. based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction （RT-PCR） results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pmsl（t）. These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pmsl（t） gene itself.

关键词： Super hybrid rice pmsl(t) Photoperiod and thermo-sensitive male sterile gene Genetic mapping

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Studies on the Rice LEAF INCLINATION1 （LC1）, an IAA-amido Synthetase, Reveal the Effects of Auxin in Leaf Inclination Control

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Molecular Plant 2013年第1期6卷 174-187页

作者： Shu-qing Zhao Jing-Jing Xiang Hong-Wei xue National Key Laboratory of Plant Molecular Genetics institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences ChineseAcademy of Sciences Shanghai 200032 China

The angle of rice leaf inclination is an important agronomic trait and closely related to the yields and archi- tecture of crops. Although few mutants with altered leaf angles have been reported, the molecular mechanism remains to be elucidated, especially whether hormones are involved in this process. Through genetic screening, a rice gain-of- function mutant leaf inclination1, Icl-D, was identified from the Shanghai T-DNA Insertion Population （SHIP）. Phenotypic analysis confirmed the exaggerated leaf angles of Icl-D due to the stimulated cell elongation at the lamina joint. LC1 is transcribed in various tissues and encodes OsGH3-1, an indole-3-acetic acid （IAA） amido synthetase, whose homolog of Arabidopsis functions in maintaining the auxin homeostasis by conjugating excess IAA to various amino acids. Indeed, recombinant LC1 can catalyze the conjugation of IAA to Ala, Asp, and Asn in vitro, which is consistent with the decreased free IAA amount in Icl-D mutant. Icl-D is insensitive to IAA and hypersensitive to exogenous BR, in agreement with the microarray analysis that reveals the altered transcriptions of genes involved in auxin signaling and BR biosynthesis. These results indicate the crucial roles of auxin homeostasis in the leaf inclination control.

关键词： rice leaf inclination auxin GH3.

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New Approach to Find Exact Solutions to Classical Boussinesq System

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Communications in Theoretical Physics 2005年第4X期44卷 597-603页

作者： ZHI Hong-Yan ZHAO xue-Qin ZHANG Hong-qing Department of Applied Mathematics Dalian University of Technology Dalian 116024 China Department of Mathematics Qufu Normal University Qufu 273165 China

In this paper, based on a new system of three Riccati equations, we give a new method to construct more new exact solutions of nonlinear differential equations in mathematical physics. The classical Boussinesq system is chosen to illustrate our method. As a consequence, more families of new exact solutions are obtained, which include solitary wave solutions and periodic solutions.

关键词： classical Boussinesq system solitary solution periodic solution

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Nickel-catalyzed cross-coupling reaction of alkynyl bromides with Grignard reagents

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Chinese Chemical Letters 2014年第9期25卷 1296-1300页

作者： qing-Han Li Yong Ding xue-Jun Yang College of Chemistry and Environmental Protection Engineering Southwest University for Nationalities

We describe a convenient method for the synthesis of 1,2-disubstituted acetylenes via a cross-coupling reaction of （bromoethynyl）benzene with Grignard reagents. The reaction of （bromoethynyl）benzene （1 mmol） with Grignard reagent （1.3 mmol） mediated by NiCl2 （4 mol%） and （p-CH3Ph）3P （8 mol%） in THF could produce 1,2-disubstituted acetylenes in good yields at room temperature.

关键词： 1,2-Disubstituted acetylene Nickel (Bromoethynyl)benzene Grignard reagent Cross-coupling

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Effects of concave and convex substrate curvature on cell mechanics and the cytoskeleton

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Chinese Chemical Letters 2017年第4期28卷 818-826页

作者： Shuang-Shuang Chen xue-Min Lu qing-Hua Lu School of Chemistry and Chemical Engineering State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong University Shanghai 200240 China

In order to understand how cells respond to concave and convex subcellular surface structures,colloidal crystal array and honeycomb-structured surfaces composed of highly ordered hexagonal units with completely inverse curvature were fabricated via facile self-assembly and breath figure approaches,*** influence of hexagonal surface curvature on cell fate was subsequently *** underwent more extensive spreading on the convex colloidal crystal array surface,while adhesive forces were higher on the concave honeycomb *** behaviors of cells on the different surfaces were investigated by comparing cell morphology,cellular adhesive force and cytoskeleton *** results revealed comprehensive differences in cell behavior between those on concave honeycomb surfaces and convex colloidal crystal arrays.

关键词： Colloidal crystal array Honeycomb structure Substrate curvature Cytoskeleton Adhesive force

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Local and regional flood frequency analysis based on hierarchical bayesian model in Dongting Lake basin,China

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Water Science and Engineering 2019年第4期12卷 253-262页

作者： Yun-biao Wu Lian-qing xue Yuan-hong Liu College of Hydrology and Water Resources Hohai UniversityNanjing 210098China Department of Fundamental Education Wanjiang University of TechnologyMaanshan 243031China College of Water and Architectural Engineering Shihezi UniversityShihezi 832003China

This study developed a hierarchical bayesian(HB)model for local and regional flood frequency analysis in the Dongting Lake basin,in *** annual maximum daily flows from 15 streamflow-gauged sites in the study area were analyzed with the HB *** generalized extreme value(GEV)distribution was selected as the extreme flood distribution,and the GEV distribution location and scale parameters were spatially modeled through a regression approach with the drainage area as a *** Markov chain Monte Carlo(MCMC)method with Gibbs sampling was employed to calculate the posterior distribution in the HB *** results showed that the proposed HB model provided satisfactory bayesian credible intervals for flood quantiles,while the traditional delta method could not provide reliable uncertainty estimations for large flood quantiles,due to the fact that the lower confidence bounds tended to decrease as the return periods ***,the HB model for regional analysis allowed for a reduction in the value of some restrictive assumptions in the traditional index flood method,such as the homogeneity region assumption and the scale invariance *** HB model can also provide an uncertainty band of flood quantile prediction at a poorly gauged or ungauged site,but the index flood method with L-moments does not demonstrate this uncertainty ***,the HB model is an effective method of implementing the flexible local and regional frequency analysis scheme,and of quantifying the associated predictive uncertainty.

关键词： Flood frequency analysis Hierarchical bayesian model Index flood method Generalized extreme value distribution Dongting Lake basin

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46,XX male sex reversal syndrome

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Asian Journal of Andrology 2004年第2期6卷 165-167页

作者： Jian-HongLi Tian-HuaHuang xue-WuJiang qing-DongXie ResearchCenterforReproductiveMedicine SecondAffiliatedHospitalDepartmentofPediatricSurgery ShantouUniversityMedicalCollegeShantou515041China

1 Case report46, XX male sex reversal syndrome is a rare anomaly with the characteristics of discordant chromosomal and gonadal sex. Since 1988, 5 children, aged 2-7 years, with normai height and body weight, were admitted to this hospital with hypospadias and chordee (1 perineal, 2 penoscrotal and l penile curvature with short urethra anomaly) in 4, obesity and hirsutism in 1, cryptorchid-

关键词： 46, XX male sex reversai chromosome hypospadias chordee cryptorchidism hirsutism

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Identification and Expression of Floral Organ Homeotic Genes from Alpinia oblongifolia (Zingiberaceae)

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Journal of Integrative Plant Biology 2009年第2期51卷 155-166页

作者： Yong-Mei Xia xue-Mei Gao qing-Jun Li Xishuangbanna Tropical Botanical Garden the Chinese Academy of Sciences Menglun Mengla Yunnan 666303 China

Current understanding of the classical ABC model of floral development has provided a new set of characters to evaluate floral evolution. However, what is still lacking is a clear assessment of this genetic program across monocots. Here, to investigate the evolution of members of class A and B genes in monocots, we report the sequence characteristic and transcript expression of three new MADS-box genes in Alpinia oblongifolia Hayata. Sequence and phylogenetic analysis reveals that these genes are FUL-like and AP3-1ike. Therefore, they were termed AoFL1, AoFL2 and AoAP3. AoFL1 contains the FUL motif, but AoFL2 lacks this motif. Their expression revealed by in situ hybridization may reflect the ancestral function of FUL-like genes in the specification of inflorescence and floral meristems. The AoAP3 gene contains two conserved motifs, the PI-derived and paleoAP3 motifs. The AoAP3 transcripts located to the corolla and stamen, and hybridization signals were detected in the central whorl. These expression patterns suggest that the functions of homologous organ identity genes are diversified in A. oblongifolia. The implications of these findings on the conservation of homologous gene function are discussed.

关键词： ABC model floral organ identity in situ hybridization MADS-box genes phylogenetic analysis.

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Evaluation of Vestibular Function in Diagnosis of Vestibular Schwannomas

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Current Medical Science 2021年第4期41卷 661-666页

作者： xue-qing ZHANG Tai-sheng CHEN Wei WANG Department of Otorhinolaryngology Head and Neck Surgery Tianjin First Central HospitalTianjin300192China Institute of Otolaryngology of Tianjin Tianjin300192China Key Laboratory of Auditory Speech and balance Medicine Tianjin300192China Key Clinical Discipline of Tianjin(Otolaryngology) Tianjin300192China Otolaryngology Clinical Quality Control Centre Tianjin300192China

Vestibular schwannomas(VS)are benign tumors of the vestibular *** common first symptoms are hearing loss and tinnitus,followed by imbalance,vertigo,and facial nerve *** subjective symptoms of VS patients are not consistent with the severity of vestibular lesions and the results of vestibular tests,which often interfere with clinicians’***,the main screening and diagnostic methods for VS are audiometry and magnetic resonance imaging(MRI),ignoring the evaluation of vestibular function at the source of pathological *** the development and improvement of vestibular evaluation technology and its wide application in the clinic,modern vestibular examination technology can reflect the severity and frequency of vestibular lesions and compensation from multiple perspectives,providing an objective basis for the diagnosis and treatment of vestibular *** this report,we review the results and characteristics of vestibular tests in VS patients and further clarify the clinical value of vestibular function assessment in the diagnosis and treatment of VS.

关键词： vestibular schwannomas vestibular function test vestibular compensation vertigo

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