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bull spermatozoa selected by thermotaxis exhibit high DNA integrity,specific head morphometry,and improve ICSI outcome

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Journal of Animal Science and biotechnology 2023年第3期14卷 1001-1011页

作者： Sara Ruiz-Diaz Rosane Mazzarella Paula Navarrete-Lopez raul Fernandez-Gonzalez Celia de Frutos Maria Maroto Claudia Cucala Paula beltran-brena Marta Lombo Dimitrios Rizos Alfonso Gutierrez-Adan department of Animal Reproduction INIA-CSIC28040 MadridSpain Mistral Fertility Clinics S.L Clinica TambreCalle Tambre828002 MadridSpain

background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DNA fragmentation(SDF)in mice,humans,and *** study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by *** We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of *** these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 ***,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially *** The results showed lower SDF(χ^(2),Prameters(length and width,Prated in comparison to those *** distribution of sperm subpopulations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated *** performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),Pbull sperm selection by thermotaxis has a much higher D

关键词： ART bovine Sperm morphometry Sperm selection Thermotaxis

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Rotational and translational domains of beta precipitate in aged binary Mg-Ce alloys

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Journal of Magnesium and Alloys 2021年第3期9卷 1039-1056页

作者： b.Z.Sun H.X.Zhang Y.Dong J.X.Ren Y.Tian G.M.Xie J.Tan Y.H.Sun Na Xiao School of Materials Science and Engineering Northeastern UniversityShenyang 110819China State Key Lab Rolling&Automat Northeastern UniversityShenyang 110819China Key Laboratory for Anisotropy and Texture of Materials Ministry of EducationNortheastern UniversityShenyang 110819China Analytical and Testing Center Northeastern UniversityShenyang 110819China

The structural evolution fromβ_(1)(Mg_(3)Ce)toβ(Mg_(12)Ce)precipitates,which takes place at the over-aged stage of binary Mg-Ce alloys,are investigated by high-angle annular dark-field scanning transmission electron *** structural transformation mainly occurs in the{111}_(β1)crystallographic planes,where the newly formedβlattices exhibit two categories of domain structures,namely rotational and translational *** rotational domain is composed of threeβdomains(β_(ra),β_(rb)andβ_(RC)),which are related by a 120°rotation with respect to each other around the 111_(β1)axis of theirβ_(1)parent ***{111}_(β1)crystallographic planes can provide four sets of sublattices with the same orientation for an initial nucleation ofβ*** leads to the formation of four translationalβdomains(β_(TA),β_(Tb),β_(TC)andβ_(TD)),among which any two differ by a vector of 1/6112_(β1).We deduce theoretically that there exist twenty-fourβdomains during this ***,considering the interfacial misfit,only one-third of domains can grow up and eventually formsβ***,a majority ofβribbons overlap partiallyβ_(1)plate,which is beneficial to relax interfacial strain amongβ,β_(1)andα-Mg matrix(α/β/β_(1)).The configuration of multipleβdomains can effectively regulate interfacial misfit ofα/βandβ/β_(1),which are responsible for enhancing the hardness and strength of Mg-Ce ***,this study aims to provide some clues to improve the over-aged performance of magnesium alloys by constructingβdomains and optimizing theα/β/β_(1)interface.

关键词： Magnesium alloys HAADF-STEM Rotational domain Translational domain Phase transition Interfacial misfit

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Expanded Relative Operator Entropies and Operator Valued α-Divergence

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Journal of Mathematics and System Science 2015年第6期5卷 215-224页

作者： Hiroshi Isa Masatoshi ito Eizaburo Kamei Hiroaki Tohyama MasayukiWatanabe Maebashi Institute of Technology Department of Integrated Design Engineering Maebashi Japan 1-1-3 Sakuragaoka Kanmakicho Kitakaturagi-gun Nara Japan Maebashi Institute of Technology Department of Life Science and Informatics Maebashi Japan

For strictly positive operators A and b, and for x ∈ [0,1] and r ∈[-1,1], we investigate the operator power mean A#x,rb=A1/2{（1-x）/＋x（a-1＊2bA-1/2）r}1/ra1/2 If r = O, this is reduced to the geometric operator mean A#x,rb=A1/2{（1-x）/＋x（a-1＊2bA-1/2）r}1/ra1/2 Since A #0,r b = A and A #l,r b = b, weregard A#t,rb as apath combining A and *** aim is to show the essential properties of St,r （AIb）. The Tsallis relative operator entropy by Yanagi, Kuriyama and Furuichi can also be expanded, and by using this, we can give an expanded operator valued a-divergence and obtain its properties.

关键词： Operator power mean relative operator entropy Tsallis relative operator entropy operator valued a-divergence

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SARS-CoV-2 interaction with Siglec-1 mediates trans-infection by dendritic cells

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Cellular & Molecular Immunology 2021年第12期18卷 2676-2678页

作者： Daniel Perez-Zsolt Jordana Muñoz-basagoiti Jordi Rodon Marc Elosua-bayes Dàlia raïch-Regué Cristina Risco Martin Sachse Maria Pino Sanjeev Gumber Mirko Paiardini Jakub Chojnacki Itziar Erkizia Xabier Muñiz-Trabudua Ester ballana Eva Riveira-Muñoz Marc Noguera-Julian Roger Paredes benjamin Trinité Ferran Tarrés-Freixas Ignacio blanco Victor Guallar Jorge Carrillo Juliàblanco Amalio Telenti Holger Heyn Joaquim Segalés bonaventura Clotet Javier Martinez-Picado Júlia Vergara-Alert Nuria Izquierdo-Useros IrsiCaixa AIDS Research Institute 089BadalonaSpain IRTA Centre de Recerca en Sanitat Animal(CReSAIRTA-UAB)Campus de la UAB08193Bellaterra(Cerdanyola del Vallès)Spain CNAG-CRG Centre for Genomic Regulation(CRGBarcelona Institute of Science and Technology(BIST)08028BarcelonaSpain Centro Nacional de biotecnología CSIC28049MadridSpain Division of Microbiology and Immunology Yerkes National Primate Research CenterEmory UniversityAtlantaGAUSA department of Pathology and Laboratory Medicine School of MedicineEmory UniversityAtlantaGeorgiaUSA Division of Pathology Yerkes National Primate Research CenterEmory UniversityAtlantaGeorgiaUSA Germans Trias i Pujol Research Institute(IGTP) Can Ruti Campus089BadalonaSpain barcelona Supercomputing Center(bSC) 08034BarcelonaSpain Catalan Institution for Research and Advanced Studies(ICREA) 08010BarcelonaSpain University of Vic-Central University of Catalonia(UVic-UCC) Vic08500Spain department of Integrative Structural and Computational biology Scripps ResearchLa JollaCA92037USA Universitat Pompeu Fabra(UPF) BarcelonaSpain UAb CReSA(IRTA-UAB)Campus de la UAB08193Bellaterra(Cerdanyola del Vallès)Spain departament de Sanitat i Anatomia Animals Facultat de VeterinàriaUAB08193Bellaterra(Cerdanyola del Vallès)Spain

Antigen-presenting cells(APCs)may be resistant to SARS-CoV-2 infection but still contribute to viral *** such as sialic acid-binding Ig-like lectin 1(Siglec-1/CD169)mediate the attachment of viruses to ***,we show that APCs effectively capture SARS-CoV-2 within compartments via recognition of *** receptor interacts with sialylated gangliosides on membranes of SARS-CoV-2 variants.

关键词： infection Antigen media

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