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Seed protein, oil, fatty acids, and minerals concentration as affected by foliar K-glyphosate applications in soybean cultivars

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Agricultural Sciences 2012年第6期3卷 848-853页

作者： manju Pande Mudlagiri b. Goli Tyneiseca Epps Nacer bellaloui Crop Genetics research Unit USDA-ARS MS 38776 USA Department of Natural Sciences and Environmental Health Mississippi Valley State University MS USA

Previous studies showed that glyphosate (Gly) may chelate cation nutrients, including potassium (K), which might affect the nutritional status of soybean seed. The objective of this study was to evaluate seed composition (protein, oil, fatty acids, and minerals) as influenced by foliar applications of K + Gly. A greenhouse experiment was conducted at Mississippi Valley State University, using two glyphosate-resistant soybean cultivars DK 4968 and Pioneer 95Y70 grown in a randomized complete block design. The treatments were foliar applications of K alone, Gly alone, K + Gly combined, and nontreated control (C). A single application of potassium (1.75% as K2SO4) was applied, and Gly was applied at a rate of 0.75 ae/ha at V5 stage. Leaf samples were harvested one week after treatment (1WAT) and 3WAT. Mature seeds were collected at harvest maturity (r8). The results showed that K, nitrogen (N), and phosphorus (P) concentrations increased in leaves in K alone and K + Gly treatments at 1WAT, but significantly increased at 3WAT in all treatments. The concentration of iron (Fe) and zinc (Zn) showed a decrease in leaf concentration in Gly and K + Gly treatments compared to C. boron (b) concentration increased in Gly treatment. Seed protein percentage was higher in all treatments in cultivar DK 4968, and the increase was about 4.0% in K treatment, 6.9% in Gly treatment, and 3.5% in K + Gly treatment compared to C. The opposite trend was observed in oil concentration, especially in Gly treatment where the percentage decrease was 11.2% compared to C. Stearic fatty acid was significantly higher in K + Gly treatment compared to K treatment for DK 4968. A higher percentage increase in linolenic acid was observed in DK 4968 in K treatment (an increase of 24.5%) and in K + Gly treatment (an increase of 29.5%) compared to C. In Pioneer 95Y70, the decrease in oil was 2.7% in K treatment and 2.3% in K + Gly treatment compared to C. Stearic acid in Pioneer 95Y70 was significantly highe

关键词： Fatty Acids Glyphosate Nutrition Oil Potassium Protein Seed Composition Soybean

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Effects of Soil Applications of Micro-Nutrients and Chelating Agent Citric Acid on Mineral Nutrients in Soybean Seeds

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Agricultural Sciences 2015年第11期6卷 1404-1411页

作者： Mudlagiri b. Goli manju Pande Nacer bellaloui Daniele De Wrachien Department of Natural Sciences and Environmental Health Mississippi Valley State University Itta Bena MS USA USDA ARS Crop Genetics Research Unit Stoneville MS USA Department of Agricultural Engineering State University of Milano Milano Italy

Micro-nutrient deficiency in soil results in crop yield loss and poor seed quality. Correcting this deficiency is normally done by foliar or soil application. The objective of this research was to determine the effects of soil applications of five micro-nutrients (Mn, Cu, Zn, Mo, and b) alone and in combination with a chelating agent citric acid (CA) on soybean leaf and seed nutrients. Source of micro-nutrient compounds were MnCl2, CuCl2, ZnCl2, MoO3, and H3bO3. Our hypothesis was that micro-and macro-nutrients may be transported to leaves and then to seeds at different rates. They may interact synergistically or competitively during the uptake process. A greenhouse experiment was conducted at Mississippi Valley State University, Itta bena, Mississippi, USA. Soybean cultivar, bolivar (maturity group V), was applied with micro-nutrients-chelating agent citric acid at V3 and r3 (pod initiation) stage. The results showed that applications of Cu, Zn, b and Mo increased three unrolled trifoliate leaves Cu, Zn, b by 26.5%, 13.8%, 113% and Mo increased to 179 mg/kg, respectively in the leaves. Also, the application of “Cu, Zn, b and Mo increased Cu, Zn, b by 55.5%, 8.2%, 28.6% and Mo increased to 202 mg/kg” respectively in soybean seeds. Application of Mn had no direct effect on increasing Mn either in leaves or in seeds, however, Mn and Mn + CA treatment affected other mineral contents. Application of Cu, Zn, Mo, b and CA increased macro-nutrients K, N, P, Mg, and S. Irrespective of the applications, the nutrient increase trend in seed was Na > Fe > Zn > Mn > b > Cu > Mo. However, Mo application resulted in the following seed nutrient accumulation pattern: Na > Mo > Fe > Zn > Mn > b > Cu. This may suggest that Mo had higher mobility to seeds than other micro-nutrients. Combination of soil application of Mo + CA increased Mo in leaves at V3 stage;however, Mo + CA soil application during pod-filling stage had no significant effects on Mo accumulation in seeds. The current r

关键词： Chealting Agent Citric Acid Micro-Element Applications Seed Minerals Soybean Seeds

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Structural,magnetic,and acidic properties of cobalt ferrite nanoparticles synthesised by wet chemical methods

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Journal of Advanced Ceramics 2015年第3期4卷 199-205页

作者： manju KUrIAN Smitha THANKACHAN Divya S.NAIr Aswathy E.K. Aswathy bAbU Arathy THOMAS binu KrISHNA K.T. Department of Chemistry Mar Athanasius CollegeKothamangalam-686666India Department of Physics Mar Athanasius CollegeKothamangalam-686666India

A detailed investigation on the effect of preparation method on the structural,magnetic,and acidic properties of cobalt ferrite nanoparticles prepared by sol-gel and co-precipitation is *** acid and ethylene glycol were used as gelling agents,while sodium hydroxide and aqueous ammonia were used as precipitating *** resulting ferrites were calcined at 450℃ and 750℃.Sharper X-ray diffraction(XrD)peaks were observed for the samples calcined at 750℃,indicating greater crystallinity of the samples calcined at higher *** crystallite sizes fell in the ranges of 7.1-21.1 nm and 30.4-42.1 nm for the samples calcined at 450℃ and 750℃,*** infrared spectra revealed two main absorption bands,the high frequency bandν1 around 600 cm^(-1) and the low frequency bandν2 around 400 cm^(-1) arising from stretching vibrations of the oxygen bond with the metal in the tetrahedral(A)and octahedral(b)sites in the spinel *** of particles was observed in the scanning electron microscopy(SEM)*** parameters of CoFe_(2)O_(4) nanoparticles greatly depended on calcination temperature and preparation *** temperature programmed desorption(TPD)measurements indicated that weak acid sites predominate medium strength sites,while the number of strong acid sites is the *** acidity decreased for the samples calcined at higher *** results underline the effect of preparation conditions on the morphology,crystallite size,and magnetic properties of nano ferrites.

关键词： nano ferrites sol-gel technique co-precipitation magnetic property surface acidity

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The role of Metal Ions in Protein and Fatty Acids biosynthesis in Soybean under Micronutrients Application to Soil

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Agricultural Sciences 2018年第6期9卷 741-749页

作者： Mudlagiri b. Goli Pande manju Kibet Daniel Nacer bellaloui Daniele De Wrachien Natural Sciences and Environmental Heath Mississippi Valley State University Itta Bena MS USA USDA Agricultural Research Service Crop Genetics Research Unit Stoneville MS USA Department of Agricultural Engineering State University Milan Italy

The present study is part of our ongoing investigation to study the role of trace elements on soybean seed composition (protein, oil, and fatty acids). This study was conducted to study the effects of five trace elements (Mn, Cu, Zn, Mo, b). The treatments of Mn, Cu, Zn, Mo, and b were chlorides, except Mo as oxide, and b as boric acid. The treatments were Mn, Cu, Zn, Mo, and b alone and in combination with the chelating agent citric acid (CA), for example Mn + CA, Cu + CA, and Zn + CA. Soybean cultivar (bolivar with maturity group V) was grown in a repeated greenhouse experiment in a randomized complete block design. The compounds were applied to three-week-old soybean plants at V3 (vegetative) and at r3 (beginning of seed-pod initiation) stages. The plants were allowed to grow until maturity under greenhouse conditions. The harvested seeds were analyzed for mineral, protein, and fatty acid contents. results showed that Mn, Cu, and b treatments increased seed protein, while Zn, Mo, Cu + CA, and b + CA decreased the protein. Treatments of Zn, Mo, CA, Cu + CA, Zn + CA, Mo + CA, and b + CA increased the oil. Treatments of Mn and Cu decreased the oil. The Cu and b treatments increased oleic acid by 8.0% and 7.4%, respectively for Cu and b. Treatments of Mn, Mo, CA, and Mn + CA, Cu + CA, Zn + CA, Mo + CA, and b + CA decreased oleic acid by 0.6% to 14.4%. Treatments of Cu, Zn, Mo, b, CA, Mn and their combination with CA increased linoleic acid by 1.3% to 6.5%. Our goal was to identify the trace elements that would make desirable alteration in the seed composition qualities.

关键词： Soybean Cu Mn Zn Mo and b Citric Acid (CA) Protein Oil and Fatty Acids

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The Effects of Essential Minerals in Different Cations and Anion Forms on Assimilation of Macro and Micronutrients in Soybean Leaves

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Agricultural Sciences 2020年第3期11卷 332-341页

作者： Goli Mudlagiri Pande manju Mann Marderius Natural Sciences and Environmental Health Mississippi Valley State University Itta Bena MS USA

In our present study, we have investigated the acquisition of minerals into soybean leaves as affected by application of various minerals in different forms of positive and negative ions. A greenhouse experiment was conducted including the following 11 treatments applied to the soil: potassium nitrate (KNO3), potassium iodide (KI), potassium chloride (KCl), sodium chloride (NaCl), calcium, magnesium and ammonium nitrates [Ca(NO3)2, Mg(NO3)2 and NH4NO3] and ammonium dihydrogen phosphate NH4(H2PO4), Na2SO4, ammonium sulfate (NH4)2SO4, and disodium phosphate Na2(HPO4). besides essential minerals, KI was also included as part of this comprehensive study, since according to some reports, molecular iodine (I2) and KI may have positive effect on growth and increased stress tolerance in some species of plants. Soybean seeds were sown in pots arranged in twelve groups/3 replicates in each group including control. At V3 stage the plants were treated with 30 mmol/pot of the proper chemicals. Ten days after the treatments, leaves were sampled and were dried, analyzed for 5 macro (K, P, S, Ca & Mg) and micronutrients (b, Cu, Fe, Mn, Zn and Na). The results showed that of all the 11 treatments, KI (T4) application increased the maximum number of mineral nutrients in leaves, which increased K by 31.9, Mg by 8, P by 60.8, S by 38.7 respectively. KI treatment also reported increased micro minerals by b, Cu, and Zn were 53.6%, 36.3% and 10.8% respectively. Similarly, KNO3 (T1) application increased several macro and micronutrients in leaves. We made an attempt to investigate the effects of various other cations like K1+ vs Na1+, and NH+ 4, Ca2+ vs Mg2+;and Anion comparisons I1- vs NO1-3, Cl1-, H2PO14;?SO2-4? ?vs (HPO4)2- in the present investigation. After reviewing our results, of all the 11 (eleven), treatments studied in our present study, T1 (KNO3), T4 (KI) and T10 Ca(NO3)2 applications proved to be the most efficient treatments by affecting the uptake of several minerals in soyb

关键词： Cations Anions Macronutrients Micronutrients Essential Minerals

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<i>In Vitro</i>Anticancer Activity of Plant-Derived Cannabidiol on Prostate Cancer Cell Lines

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Pharmacology & Pharmacy 2014年第8期5卷 806-820页

作者： manju Sharma James b. Hudson Hans Adomat Emma Guns Michael E. Cox Department of Pathology & Laboratory Medicine University of British Columbia Vancouver Canada The Vancouver Prostate Centre 2660 Oak St. Jack Bell Research Centre Vancouver Canada The Vancouver Prostate Centre 2660 Oak St. Jack Bell Research Centre Vancouver Canada Department of Urologic Sciences Faculty of Medicine University of British Columbia Vancouver Canada

Cannabinoids, the active components of Cannabis sativa Linnaeus, have received renewed interest in recent years due to their diverse pharmacologic activities such as cell growth inhibition, anti-inflammatory effects and tumor regression, but their use in chemotherapy is limited by their psychotropic activity. To date, cannabinoids have been successfully used in the treatment of nausea and vomiting, two common side effects that accompany chemotherapy in cancer patients. Most non-THC plant cannabinoids e.g. cannabidiol and cannabigerol, seem to be devoid of psychotropic properties. However, the precise pathways through which these molecules produce an antitumor effect have not yet been fully characterized. We therefore investigated the antitumor and anti-inflammatory activities of cannabidiol (CbD) in human prostate cancer cell lines LNCaP, DU145, PC3, and assessed whether there is any advantage in using cannabis extracts enriched in cannabidiol and low in THC. results obtained in a panel of prostate cancer cell lines clearly indicate that cannabidiol is a potent inhibitor of cancer cell growth, with significantly lower potency in non-cancer cells. The mrNA expression level of cannabinoid receptors Cb1 and Cb2, vascular endothelial growth factor (VEGF), PSA (prostate specific antigen) are significantly higher in human prostate cell lines. Treatment with Cannabis extract containing high CbD down regulates Cb1, Cb2, VEGF, PSA, pro-inflammatory cytokines/chemokine IL-6/IL-8. Our overall findings support the concept that cannabidiol, which lacks psychotropic activity, may possess anti-inflammatory property and down regulates both cannabinoid receptors, PSA, VEGF, IL-6 and IL-8. High CbD cannabis extracts are cytotoxic to androgen responsive LNCaP cells and may effectively inhibit spheroid formation in cancer stem cells. This activity may contribute to its anticancer and chemosensitizing effect against prostate cancer. Cannabidiol and other non-habit forming cannabinoids c

关键词： Prostate Cancer Androgen receptor Cannabidiol (CbD) Anti-Inflammatory Cb1 Cb2 Prostate Cancer Cell Lines

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EFFECTS OF POPULATION AND INDUSTrIALIZATION ON THE DEGrADATION OF bIOMASS AND ITS rEGENErATION bY AFFOrESTATION: A MATHEMATICAL MODEL

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生物数学学报 1988年第1期 1-9页

作者： J.b.Shukla V.N.Pal O.P.Misra manju Agarwal Ajai Shukla Departmant of Mathematics I.I.T Kanpur India Departmant of Mathematics I.I.T Kanpur India Departmant of Mathematics I.I.T Kanpur India Departmant of Applied Science Institute of Eng.and Techn Lucknow India Nodal Centre for National Technical Manpower Information System I. I. T. Kanpur India

The bio-mass(Food, Fodder and Forestry, etc. ) in a habitat has beencontinuously depleting due to rapid industrialization and over-population leadingto various kinds of undesirable effects on our environment and on land masscausing deep concern to planners and management experts. In this paper, therefore, a mathematical model for degradation of bio-massis presented to study the effects of industrialization and associated growth ofpopulation, It is shown that with the increase in the levels of these factors,the bio-mass resource may not last long. It has also been pointed out mathematicallythat the level of the biomass can be maintained at desired equilibrium level bya suitable afforestation programme.

关键词： A MATHEMATICAL MODEL EFFECTS OF POPULATION AND INDUSTrIALIZATION ON THE DEGrADATION OF bIOMASS AND ITS rEGENErATION bY AFFOrESTATION

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A Polymerase Chain reaction based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate reducing bacteria

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Journal of Environmental Protection 2014年第12期5卷 1111-1115页

作者： Anita Shete Vikram Ghole manju raina bikash Aich Aavanira biotech Pvt. Ltd. Pune India Department of biochemistry University of Pune Pune India Ministry of Environment & Forests New Delhi India

Genetic profiling of environmentally important organisms is very essential for easy identification of biodegrading bacteria. In the previous study, we have reported the perchlorate biodegrading bacteria and characterized them by biochemical analysis and 16 S sequencing. We have observed a very similar isolates of Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium perchlorate [1] 08D0C9EA79F9bACE118C8200AA004bA90b02000000080000000E0000005F005200650066003300390038003100390037003300340037000000 . In this study, we report PCr based DNA fingerprinting technique to generate the genomic signature of these closely related group of Arthrobacter species. This study also effectively generates unique genomic signature for each of these isolates that has potential for use in molecular monitoring as well as for tracking genomic variation and rearrangements.

关键词： AFLP—Amplified Fragment Length Polymorphism Arthrobacter Ammonium Perchlorate bio-Degradation

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Effects of chelating agents on protein, oil, fatty acids, and minerals in soybean seed

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Agricultural Sciences（农业科学） 2012年第4期

作者： Mudlagiri b. Goli manju Pande Nacer bellaloui

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Seed protein, oil, fatty acids, and minerals concentration as affected by foliar K-glyphosate applications in soybean cultivars

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Agricultural Sciences（农业科学） 2012年第6期

作者： manju Pande Mudlagiri b. Goli Tyneiseca Epps Nacer bellaloui

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