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Characterization,expression dynamics,and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

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Journal of Oceanology and Limnology 2024年第1期42卷 187-200页

作者： Xinming GAO binbin FENG Chen DU Congcong hou Shan JIN Daojun TANG Junquan ZHU Key Laboratory of Applied Marine biotechnology by the Ministry of Education and Key Laboratory of Marine biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China College of Ecology Lishui UniversityLishui 323000China

Mitochondria undergo morphological changes during spermatogenesis in some *** mechanism and role of mitochondrial morphology regulation,however,remain somewhat *** this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta *** full-length cDNA of the *** opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino *** Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting *** and protein expression was higher in the coelomic fluid(a site of spermatid development)of male *** and increased first and then decreased from March to ***,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in *** *** Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic ***,Pe-OPA1 was mainly detected in the spermatid *** experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in *** *** results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.

关键词： optic atrophy protein 1(OPA1) spermiogenesis Phascolosoma esculenta mitochondrial dynamics spermatid

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Endoplasmic reticulum stress-induced NLRP3 inflammasome activation as a novel mechanism of polystyrene microplastics (PS-MPs)-induced pulmonary inflammation in chickens

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Journal of Zhejiang University-Science b(biomedicine & biotechnology) 2024年第3期25卷 233-243页

作者： Hongmin LU Tiantian GUO Yue ZHANG Dewang LIU Lulu hou Chengxue MA Mingwei XING College of Wildlife and Protected Area Northeast Forestry UniversityHarbin 150040China

Microplastics(MPs)have attracted growing attention worldwide as an increasingly prevalent environmental *** addition,chicken meat is currently the most widely consumed kind of poultry in the global *** demand for chicken is on the rise both at home and *** a result,the safety of chicken raising has also received significant *** lungs play an essential role in the physiological activities of chickens,and they are also the most vulnerable *** injury is difficult to repair after the accumulation of contaminants,and the mortality rate is high,which brings huge economic losses to *** research on the toxicity of MPs has mainly focused on the marine ecosystem,while the mechanisms of toxicity and lung damage in chickens have been poorly ***,this study explored the effects of exposure to polystyrene microplastics(PS-MPs)at various concentrations for 42 d on chicken ***-MPs could cause lung pathologies and ultrastructural abnormalities,such as endoplasmic reticulum(ER)swelling,inflammatory cell infiltration,chromatin agglutination,and plasma membrane ***,PS-MPs increased the expression of genes related to the heat shock protein family(Hsp60,Hsp70,and Hsp90),ER stress signaling(activating) transcription factor 6(ATF0),ATF4,protein kinase RNA-like ER kinase(PERK),and eukaryotic translation initiation factor 2 subunitα(eIF2a),pyroptosis-related genes(NOD)-,LRR-and pyrin domain-containing protein 3(NLRP3),apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),interleukin-1β(IL-1β),cysteinyl aspartate-specific proteinase 1(Caspasel),and gasdermin-D(GSDMD),and the inflammatory signaling pathway(nuclear factor-kb(NF-kb),inducible nitric oxide synthase(iNOS),and cyclooxygenase-2(COX-2).The above results showed that PS-MP exposure could result in lung stress,ER stress,pyroptosis,and inflammation in *** findings provide new scientific clues for further research o

关键词： Polystyrene microplastics Endoplasmic reticulum stress Lung NLRP3 inflammasome Inflammation

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Promoted monolithic perovskite/organic tandem solar cells through elaborate manipulation of light transmission and carrier tunneling in interconnect junction

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Science China Chemistry 2024年第9期67卷 3131-3139页

作者： Rongbo Wang Jiawei Zhang Juntao Zhao Ya Wang Yi Ding Ying Zhao Xiaodan Zhang Guofu hou Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage CenterSolar Energy Conversion CenterNankai UniversityTianjin 300350China Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Tianjin 300350China Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Tianjin 300350China State Key Laboratory of Photovoltaic Materials and Solar Cells Tianjin 300350China

Monolithic perovskite/organic tandem solar cells(TSCs)have emerged as promising thin film solar *** is recognized that interconnect junction plays a pivotal role in tandem ***,wide bandgap Cs_(0.25)FA_(0.75)Pb(I_(0.6)br_(0.4))_(3)perovskite top-cell and narrow bandgap PM6:Y6:PC_(61)bM ternary organic bottom-cell were integrated in this study with several kinds of thin metal interconnect layers,which provides feasibility to elaborately manipulate light transmission and carrier tunneling process in interconnect *** is confirmed that,in comparison with Au,employing an Ag interconnect layer elevates integrated transmittance of light in longer wavelength regions,mainly because of the alleviated screening effect with a lower free electron concentration,which offers sufficient light harvest for the ***,established energy barriers with moderate height afford convenient extraction and recombination for both holes and ***,the performance of TSCs is promoted ***,an innovative Ag/Au double interconnect layer is proposed accordingly,which can preserve exceptional conductivity and light transmission and further reduce barrier height,especially for hole tunneling,by optimizing the band alignment between the interconnect layer and ***,the monolithic perovskite/organic TSC with a striking efficiency of 23.26% is *** a word,this study can pave a general approach toward high-performance TSCs integration.

关键词： tandem solar cells band alignment perovskite organic solar cells interconnect junction

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Laser constructed vacancy-rich TiO_(2-x)/Ti microfiber via enhanced interfacial charge transfer for operando extraction-SERS sensing

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Chinese Chemical Letters 2024年第9期35卷 187-192页

作者： Ying hou Zhen Liu Xiaoyan Liu Zhiwei Sun Zenan Wang Hong Liu Weijia Zhou Institute for Advanced Interdisciplinary Research(iAIR) School of Chemistry and Chemical EngineeringUniversity of JinanJi’nan 250022China State Key Laboratory of Crystal Materials Shandong UniversityJi’nan 250100China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China

Semiconductor-molecule surface-enhanced Raman scattering(SERS),especially the stronger interfacial charge transfer process(ICTP),represents a frontier in the field of SERS with spectral reproducibility and unparalleled ***,through a laser microfabrication method in situ,the free-standing,super hydrophilic and vacancy-rich TiO_(2-x)/Ti is successfully *** blue TiO_(x)/Ti(b-TiO_(x)/Ti)as preconcentrated substrate,a nanomolar-level limit of detection of 12 nmol/L at 1385 cm–1,is confirmed using crystal violet(CV)bacteriostat as a model under 532 nm ***,the results demonstrate that the SERS enhancement mechanism is via the moderate adulteration of oxygen vacancy,which leads to a narrow value of band gap and increases the ICTP of substrate to *** a hand-held extractor assembled with b-TiO_(x)/Ti microfiber,the operando analysis of mixtures distributed information excited in different parts of Asian carp is facilely *** work guides the controlled synthesis of vacancy-rich TiO_(2-x)/Ti nanostructure and its application in ultrasensitive extraction-SERS *** also provides the direction for the rapid and operando transmission of biological information with temporal and spatial concentration distribution in human tissues by highly sensitized materials.

关键词： Defect engineering TiO_(2-x)/Ti microfiber Laser microfabrication Surface-enhanced Raman scattering Solid phase microextraction Operando sensing

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Effect of micro-clearance structure on the collapse of individual liquid hydrogen bubbles

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Energy Storage and Saving 2024年第3期3卷 231-241页

作者： Shaohang Yan Qi Zhao Tianwei Lai Yu hou School of Energy and Power Engineering Xi’an Jiaotong UniversityXi’an710049China MOE Key Laboratory of Cryogenic Technology and Equipment Xi’an Jiaotong UniversityXi’an710049China

Cavitation occurs in the micro-clearance of liquid-hydrogen-lubricated bearings owing to the pressure drop caused by high-speed *** pressure undulation caused by cavitation collapse results in bearing surface erosion and significantly affects the bearing *** this study,a modified Z-G-b cavitation model was used to study the crushing process of a single liquid hydrogen bubble in a shear *** Fourier transform(FFT)and wavelet transform(WT)were applied to study the frequency characteristics of the pressure,mass transfer rate,and vapor mass fraction during bubble rupture in shearing *** obtain a deeper insight into the details of the effect of the shear micro-clearance structure on bubble collapse,the relationship between the flow field energy,attenuation rate,and frequency was *** proper orthogonal decomposition(POD)and dynamic mode decomposition(DMD)methods were used to analyze the energy of each order mode of the flow *** analysis results of the bubble vibration intensity with respect to time and frequency provide a theoretical basis for the optimization of the bearing structure.

关键词： Liquid hydrogen bubble collapse Shearing micro-clearance Wavelet transform Flow field analysis

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Experimental investigation on pyrolysis products and pore structure characteristics of organic-rich shale heated by supercritical carbon dioxide

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Petroleum Science 2024年第4期21卷 2393-2406页

作者： bai-Shuo Liu Chuan-Jin Yao Jia-Long Qi Ya-Qian Liu Liang Xu Jing-Xuan hou State Key Laboratory of Deep Oil and Gas China University of Petroleum (East China) Key Laboratory of Unconventional Oil & Gas Development (China University of Petroleum (East China)) Ministry of Education School of Petroleum Engineering China University of Petroleum (East China)

The efficient pyrolysis and conversion of organic matter in organic-rich shale,as well as the effective recovery of pyrolysis shale oil and gas,play a vital role in alleviating energy *** state of carbon dioxide（CO2） in the pyrolysis environment of shale reservoirs is the supercritical *** unique supercritical fluid properties not only effectively heat organic matter,displace pyrolysis products and change shale pore structure,but also achieve carbon storage to a certain *** samples were made into powder and three sizes of cores,and nitrogen（N2） and supercritical carbon dioxide（ScCO2）pyrolysis experiments were performed at different final pyrolysis *** properties and mineral characteristics of the pyrolysis products were studied based on gas chromatography analysis,Xray diffraction tests,and mass spectrometry ***,the pore structure characteristics at different regions of cores before and after pyrolysis were analyzed using N2adsorption tests to clarify the impact of fracturing degree on the pyrolysis *** results indicate that the optimal pyrolysis temperature of Longkou shale is about 430℃.Compared with N2,the oil yield of ScCO2pyrolysis is *** pyrolysis oil obtained by ScCO2extraction has more intermediate fractions and higher relative molecular *** ScCO2can effectively improve the pore diameter of shale and its effect is better than that of *** micropores are produced in shale after pyrolysis,and the macropores only are generated in ScCO2pyrolysis environments with temperatures greater than 430℃.The pore structure has different development characteristics at different pyrolysis temperatures,which are mainly affected by the pressure holding of volatile matter and products *** to the surface of the core,the pore development effect inside the core is *** the decrease in core size,the pore diameter,specific surface area,and pore volume of cores all increase after pyrolysis.

关键词： Organic-rich shale Supercritical carbon dioxide In-situ pyrolysis Pore structure Core size and region

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Enhanced photocatalytic performance of bi_(2)O_(2)CO_(3)/bi_(4)O_(5)br_(2)/reduced graphene oxide Z-schemehe terojunction via a one-pot room-temperature synthesis

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Journal of Environmental Sciences 2024年第4期138卷 418-427页

作者： Xiaoge Wu Lei Yan Rongrong Qin Qikai Zhang Wei Yang Xiaozhi Wang Yongcai Zhang Min Luo Jianhua hou College of Environmental Science and Engineering Yangzhou UniversityYangzhouJiangsu 225009China Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization Nanjing 210095China beijing Xinfeng Aerospace Equipment Co. Ltd.Beijing 100854China beijing System Design Institute of Electro-Mechanic Engineering Beijing 100005China College of Chemistry and Chemical Engineering Ningxia UniversityYinchuanNingxia 750021China

bi_(2)O_(2)CO_(3)(bOC)/bi_(4)O_5br_(2)(bOb)/reduced graphene oxide(rGO)Z-scheme heterojunction with promising photocatalytic properties was synthesized via a facile one-pot room-temperature ***-thin nanosheets of bOC and bOb were grown in situ on r *** formed 2D/2D direct Z-scheme heterojunction of bOC/bOb with oxygen vacancies(OVs)effectively leads to lower negative electron reduction potential of bOb as well as higher positive hole oxidation potential of bOC,showing improved reduction/oxidation ***,rGO is an acceptor of the electrons from the conduction band of *** dual roles significantly improve the transfer performance of photo-induced charge carriers and accelerate their *** layered nanosheet structure,rich OVs,high specific surface area,and increased utilization efficiency of visible light,the multiple synergistic effects of bOC/bOb/rGO can achieve effective generation and separation of the electron-holes,thereby generating more·O_(2)^(-)and h^(+).The photocatalytic reduction efficiency of CO_(2)to CO(12.91μmol/(g·hr))is three times higher than that of bOC(4.18μmol/(g·hr)).Moreover,it also achieved almost 100%removal of Rhodamine b and cyanobacterial cells within 2 hours.

关键词： bi_(2)O_(2)CO_(3) bi_(4)O_(5)br_(2) Reduced graphene oxide Z-scheme heterojunction Photocatalysis CO_(2)reduction

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Thermo-Flow Performances for the Main-Auxiliary Integrated Natural Draft Dry Cooling System

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Journal of Thermal Science 2024年第3期33卷 1010-1025页

作者： ZHANG Zongyang hou Yichen Zhou Hanyu JIA He CHEN Lei KONG Yanqiang WANG Weijia YANG Lijun DU Xiaoze Key Laboratory of Power Station Energy Transfer Conversion and System of Ministry of Education School of Energy Power and Mechanical EngineeringNorth China Electric Power UniversityBeijing102206China

Recently,natural draft dry cooling system with the main-auxiliary integrated air-cooled heat exchangers in the up and lower layers,has drawn attention to the electric power *** research firstly develops two physical models for the integrated cooling system,namely Case A and Case *** Case A,the main air-cooled heat exchanger is arranged in the upper layer and the auxiliary air-cooled heat exchanger arranged in the lower layer,while in Case b,the two heat exchanger systems are arranged in the opposite *** then,directing at the engineering TMCR and TRL 1 working conditions,the unit-local-overall thermo-flow characteristics of Case A and Case b are obtained and compared by numerical *** findings show that,for the auxiliary air-cooled exchanger,Case A has obviously higher cooling performances than Case b,with the difference varying from 5.46%to 7.55%.Whereas,for the main air-cooled exchanger,Case b shows the recovered cooling performances,with the difference changing from 1.15%to 2.99%.Case A is preferably recommended to the engineering application in consideration of more strict cooling demand of the auxiliary cooling ***,this research will provide some theoretical guidelines for the design and construction of the main-auxiliary integrated natural draft dry cooling system.

关键词： integrated dry cooling system main air-cooled heat exchanger auxiliary air-cooled heat exchanger upper layer and lower layer thermo-flow performances

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Corrosion behavior of Graphene Nanosheets Reinforced Magnesium Matrix Composites in Simulated body Fluids

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Acta Metallurgica Sinica(English Letters) 2024年第3期37卷 525-536页

作者： Liwen Chen Jianhui Jing Lulu Zhang Jing Li Weipeng Chen Limin Li Yuan Zhao Hua hou Yuhong Zhao School of Materials Science and Engineering Collaborative Innovation Center of Ministry of Education and Shanxi Province for High-Performance Al/Mg Alloy MaterialsNorth University of ChinaTaiyuan 030051China Shanxi Key Laboratory of Advanced Manufacturing Technology School of Mechanical EngineeringNorth University of ChinaTaiyuan 030051China School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China Institute of Materials Intelligent Technology Liaoning Academy of MaterialsShenyang 110004China

Magnesium(Mg)alloy is considered as a promising biodegradable implant material but restricted to rapid ***,the new strategies based on thixomolding process had been explored to utilize the outstanding anti-permeability of graphene nanosheets(GNPs)while inhibit its galvanic corrosion with the matrix,so as to improve the corrosion resistance of *** agglomerate of GNPs with 0.9 wt%content is the main reason for the deterioration of corrosion performance due to the formation of micro-galvanic *** grain refinement of composites with 0.6 wt%content had positive effects on the better corrosion *** process adjusting,the unique distributions of GNPs along grain boundaries play a vital role in improving the corrosion *** can be ascribed to the following mechanisms:(I)The barriers can be established between the Mg matrix and corrosive medium,hence blocking the charge transfer at the interface;(II)The GNPs can effectively promote apatite deposition on the Mg matrix,leading to form dense apatite layers and prevent the further invasion of SbF;(III)The GNPs acting as reinforcements exists in the corrosion layer and apatite layer,impede the apatite layer falling off from the Mg *** findings broaden the horizon for biomedical applications in Mg matrix composites to realize desired performances.

关键词： Magnesium matrix composite Graphene nanosheets Corrosion resistance

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Recent Advances in the Electrochemical Defluorinative Transformations of C-F bonds

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Chinese Journal of Chemistry 2024年第16期42卷 1913-1928页

作者： Qinhui Wan Rui-Xue Liu Zhongyi Zhang Xia-Die Wu Zhong-Wei hou Lei Wang Advanced Research Institute and Schoolof Pharmaceutical Sciences Taizhou UniversityJiaojiangZhejiang 318000China Department of Chemistry Southern University of Science and TechnologyhenzhenGuangdong 518055China College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistry and Material Technology Ministryof EducationHangzhou Normal UniversityHangzhouZhejiang 311121China

Organic fluorine compounds are ubiquitous and pivotally important organic molecules,yet their activation and transformation have long been a formidable challenge due to the high energy and low reactivity of C-F *** electrosynthesis,an environmentally benign synthetic method in organic chemistry,enables a myriad of chemical transformations without the need for external redox *** recent years,organic electrochemistry has emerged as a powerful tool for achieving the activation and transformation of C-F bonds in fluorine-containing *** review aims to succinctly recapitulate the latest advancements in the electrochemical defluorinative transformations of C-F bonds and to delve into the reaction design,mechanistic insights,and developmental prospects ofthese methods.

关键词： Organic electrochemistry Electroreduction Defluorination C-F bond activation Organic fluorine compounds

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