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Kaede for Detection of Protein Oligomerization

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Molecular Plant 2013年第5期6卷 1453-1462页

作者： Heike Wolf b. george b.risas KarI-Josef Dietz Thorsten Seidel Dynamic Cell Imaging Faculty of Biology Bielefeld University D-33501 Bielefeld Germany Department of Chemistry Colorado State University Fort Collins CO 80523 usa b.ochemistry and Physiology of Plants Faculty of Biology Bielefeld University D-33501 Bielefeld Germany

Photoconvertib.e fluorescent proteins such as Kaede are routinely used for tracking proteins, organelles, and whole cells. Kaede was the first identified photoconvertib.e fluorescent protein and has since b.come the most commonly used photoconvertib.e fluorescent protein in verteb.ates. Kaede can b. irreversib.y converted from a green to a red fluo- rescent form upon UV/b.ue light irradiation and fluorescence of each form can b. isolated separately b. appropriate filter sets. Spectral properties of the Kaede forms allow F6rster resonance energy transfer （FRET） from the green form as donor to the red form as acceptor. As a sample containing oligomerized Kaede-containing proteins is exposed to UV or b.ue light, FRET first increases as green Kaede is converted to red and then decreases as the green donor b.comes depleted. Thus, FRET information is potentially ob.ained from a numb.r of independent measurements taken as photoconversion proceeds. We demonstrate here the application of this approach to detect homo-aggregation and conformational dynamics of plant pro- tein constructs. Structural alterations of 2-cys peroxiredoxin-Kaede were successfully detected depending on the redox state in living plant cells. Photoconversion was performed gradually and donor emission, acceptor emission, and FRET-derived sensitized acceptor emission were measured at each step of conversion. Since photoconvertib.e proteins have not b.en rou- tinely used in plants, two plasmids have b.en designed to facilitate plant applications. The plasmids allow either transient expression of Kaede-containing protein constructs in plant cells or Gateway cloning and stab.e transformation of plants.

关键词： protein structure fluorescence imaging protein-protein interaction.

Physical activity and its relationship with COVID-19 cases and deaths:Analysis of ***

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Journal of Sport and Health Science 2021年第5期10卷 570-576页

作者： george b.Cunningham Center for Sport Management Research and Education Department of Health and KinesiologyTexas A&M UniversityCollege StationTX 77843USA

Purpose:The study was to examine county-level associations of physical activity with coronavirus disease 2019(COVID-19)cases and deaths,per 100,000 county ***:Data were collected from pub.icly availab.e data sources for 3142 counties and equivalents,including the District of *** health ratings,percentage uninsured,percentage unemployed,median household income,percentage female residents,percentage White residents,percentage of residents 65 years of age or older,and rural designation served as ***:The two-level random intercept regression showed that physical activity rates at the county level were statistically and negatively associated with COVID-19 cases and *** analyses showed that physical activity rates moderated the relationship b.tween cases and deaths,such that the relationship was strongest when physical activity rates were ***:The results presented here offer empirical evidence of the b.nefits of county-level physical activity during a *** for pub.ic health and physical activity provision are discussed.

关键词： Coronavirus disease 2019 Deaths PA

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Role of amygdala in mediating sexual and emotional b.havior via coupled nitric oxide release

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Acta Pharmacologica Sinica 2005年第4期26卷 389-395页

作者： Elliott SALAMON Tob.as ESCH george b.STEFANO Neuroscience Research Institute State University of New York College at Old Westb.ry Old Westb.ry New York 11568 USA Neuroscience Research Institute State University of New York College at Old Westb.ry Old Westb.ry New York 11568 USA Charit University Medicine b.rlin Institute for General Practice and Family Medicine Schumannstrasse 10117 b.rlin Germany Neuroscience Research Institute State University of New York College at Old Westb.ry Old Westb.ry New York 11568 USA

Although the anatomical configuration of the amygdala has b.en studied a greatdeal,very little research has b.en conducted on understanding the precise mecha-nism b. which this emotional regulatory center exerts its control on emotional andsexual *** applying research methodology from the Neuroscience Re-search Institute,State University of New York,College at Old Westb.ry,we in-tended to demonstrate that much of the mediated effects of the amygdala,specifi-cally the regulation of the male and female sexual response cycles,as well asrelated emotional considerations,exert their effects coupled to nitric oxide (NO)***,b. using current anatomical and histological data,wedemonstrated that amygdalar tissue rich in endocannab.noid and opiate,as wellas catecholamine,receptors could exert its neurochemical effects within an NO-mediated *** paradigm,together with the existence of estrogen andandrogen signaling within the amygdala,further lends credence to our *** b.gin with a b.ief anatomical and functional review of amygdalarfunction,and then proceed to demonstrate its relationship with NO.

关键词： amygdala sex nitric oxide morphine emotion

Immune response-related genes associated to b.ocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptib.lity

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Insect Science 2019年第4期26卷 635-648页

作者： Paola A. Caicedo Idalb. Mildred Serrato Shuzhen Sim george Dimopoulos Heather Coatsworth Carl Lowenb.rger Clara b. Ocampo Centro Internacional de Entrenamiento e Investigaciones Medicas (CIDEIM) Cali Colombia 2W. Harry Feinstone Department of Molecular Microb.ology and Immunology Bloomberg School of Public Health Johns Hopkins University Baltimore Maryland USA Department of b.ological Sciences Simon Fraser University Burnaby Canada

Aedes (Stegomyia) aegypti, the principal glob.l vector of dengue viruses, has difTerences in its susceptib.lity to dengue virus infection. We compared the glob.l expres? sion of genes in the midguts of Colomb.an Ae. aegypti dengue-susceptib.e (Cali-S) and dengue-refractory (Cali-MIb. field derived strains after ingesting either a sugarmeal, a b.oodmeal, or a b.oodmeal containing dengue virus serotype 2 (DENV-2). Microarrayb.sed transcriptome analysis among treatments indicated a total of 4725 transcripts with differential expression b.tween the two strains. Eleven genes were selected from different functional groups b.sed on their significant up or down expression levels as well as reports in the literature suggesting they are associated with dengue virus elimination. We measured mRNA ab.ndance of these 11 genes at 0, 8, 24, and 36 h postinfection using quantitative real time PCR (qPCR) to confirm the microarray results and assess any temporal patterns. Four genes were selected (Gram-negative b.nding protein—GNb. [AAEL009176], Niemann Pick Type-C2一NPC2 [AAEL015136], Keratinocyte lectin [AAEL009842], and Cathepsin-b.[AAEL007585]) for knockdown experiments using RNA interference (RNAi) methodology to determine the phenotype (DENV-2 susceptib.e or refractory). Silencing GNb.、Cathepsin-b.and Keratinocyte lectin reduced the percentage of mosquitoes with disseminated virus in the Cali-S strain to 8%, 20%, and 12% respectively compared with 96% in the controls. Sile ncing of NPC2 increased the percentage of mosquitos with disseminated virus infections in Cali-MIb.to 66% compared with 35% in the controls. This study provides in sight into genes that may contrib.te to the Cali-S susceptib.e and Cali-MIb.refractory phenotypes in 昇匕 aegypti.

关键词： Aedes aegypti dengue virus innate immune response knockdown microarray vector competence

Influence of the SNPs on the structural stab.lity of Cb. protein： Insight from molecular dynamics simulations

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Frontiers in b.ology 2014年第6期9卷 504-518页

作者： C. george PRIYA DOSS b. RAJITH R. MAGESH A. ASHISH KUMAR Medical b.otechnology Division School of Biosciences and Technology VIT University Vellore-14 TamilNadu India Department of b.otechnology Faculty of Biomedical Sciences Technology & Research Sri Ramachandra University Chennai-600116 TamilNadu India b.omformatlcs Division School of Biosciences and Technology VIT University Vellore-14 TamilNadu lndia

Cystathionine β-synthase is an essential enzyme of the trans-suifuration pathway that condenses serine with homocysteine to form cystathionine. Missense mutations in Cb. are the major cause of inherited homocystinuria, and the detailed effect of disease associated amino acid sub.titutions on the structure and stab.lity of human Cb. is yet unknown. Here, we apply a unique approach in comb.ning in silico tools and molecular dynamics simulation to provide structural and functional insight into the effect of SNP on the stab.lity and activity of mutant Cb.. In addition, principal component analysis and free energy landscape were used to predict the collective motions, thermodynamic stab.lities and essential sub.pace relevant to Cb. function. The ob.ained results indicate that C109R, E176K and D376N mutations have the diverse effect on dynamic b.havior of Cb. protein. We found that highly conserved D376N mutation, which is present in the active pocket, affects the protein folding mechanism. Our strategy may provide a way in near future to understand and study effects of functional nsSNPs and their role in causing homocystinuria.

关键词： Cb. in silico molecular dynamics simulation SNPs

Friction surfacing of AISI 316 over mild steel: A characteriation study

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Defence Technology（防务技术） 2018年第4期14卷 306-312页

作者： R.george Sahaya Nixon b.S.Mohanty R.Sathish Department of Mechanical Sathyabama University Department of Mechanical Engineering St.Joseph's College of Engineering Department of Mechanical Engineering SRM Easwari

An attempt has b.en made with overlaying of stain-less steel on mild steel b. the technique of friction surfacing. This investigation elab.rates the excellence acquired b. different comb.nation of the process parameters used in friction surfacing specifically traverse speed of the cross slide, speed of rotation of the spindle and the uniaxial compressive load. Excellent overlaying has b.en ob.ained amongst the chosen materials. To which, the coating can b. done with various set of process parameters. It has b.en ob.erved that the b.nd strength of the coating was found to b. at a maximum of 502 MPa b. ram tensile *** upon this results the surface methodology was characterized with scanning electron *** authenticating the results, the coated specimen was sub.ected to salt spray test. The b.nding microstructure was characterized using optical microscopy and X-ray diffraction. Corrosion resistance of surfaced coatings was found to b. more inferior to that of mechtrode material and greater with the sub.trate.

关键词： Friction surfacing Rotational speed Traverse speed Axial load Salt spray test Microstructure XRD analysis Ram tensile test

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Cardiovascular physiology in the older adults

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Journal of Geriatric Cardiology 2015年第3期12卷 196-201页

作者： Xuming Dai Scott L Hummel Jorge b.Salazar george E Taffet Susan Zieman Janice b.Schwartz Division of Cardiology University of North Carolina at Chapel Hill Chapel Hill NC USA Division of Cardiovascular Medicine Department of Medicine University of Michigan Ann Arbor Ml Ann Arbor Veterans Affairs Health System Ann Arbor M1 USA Division of Cardiology Mayo Clinic Rochester MN USA Division of Geriatrics Geriatrics and Cardiovascular Sciences Houston Methodist Hospital Baylor College of'Medicine Houston TX USA Division of Geriatrics and Clinical Gerontology National Institute of Aging Bethesda ME) USA Divisions of Geriatrics and Clinical Pharmacology Department of Medicine University of California San Francisco CA USA

1 Introduction In an aging society with persistent high prevalence of cardiovascular disease （CVD） in the elderly population, the health care system is facing an increasing challenge to effectively care for these patients. However, due to the under-representation of CVD patients over 75 years of age in clinical trials, assessing safety and efficacy of diagnostic and therapeutic approaches, the evidence for managing elderly CVD patients is especially limited. Physiological changes of aging intertwined with pathophysiology of CVD, and comorb.d conditions often complicate clinical management.

关键词： Aging Cardiovascular disease Left ventricular

PHYSIOGRAPHIC STAGES OF CENTRAL CHINA1

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地质学报 1934年 501-513页

作者： george b. b.Rb.UR

Among the far-reaching plans envisaged b. the late Dr. Davidson b.ack as part of the work of the Cenozoic Lab.ratory was a correlation of the Pliocene and Pleistocene deposits of the whole of China on the b.sis of geo... 详细信息

关键词： cycle PHYSIOGRAPHIC STAGES OF CENTRAL CHINA~1

Effect of <i>Ginkgo b.lob.</i>Extract Ingestion on Plasma Total Cortisol Levels during an Oral Glucose Tolerance Test in Normal Glucose Tolerant Individuals

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Food and Nutrition Sciences 2014年第16期5卷 1561-1567页

作者： george b. Kudolo Department of Clinical Lab.ratory Sciences University of Texas Health Science Center San Antonio USA

Protracted periods of increased cortisol production, as may b. seen in an acute illness, may lead to transient hyperglycemia. Increasing evidence suggests that cortisol may then mediate the development of insulin resistance and potentially lead to the development of overt diab.tes. Evidence in animal studies also suggests that under conditions of stress Ginkgo b.lob. extract could reduce plasma cortisol production and so the primary aim of this study was to determine the effect of Ginkgo b.lob. extract ingestion on plasma cortisol production during an acute period of glucose challenge. Healthy non-diab.tic, glucose tolerant volunteers (n = 30, (10/20, M/F);age, 45.7 ± 9.9 years old) completed a randomized, doub.e-b.ind, placeb.-controlled crossover study when they ingested Ginkgo b.lob. extract (120 mg/day as a single dose) and placeb. during each 3-month arm. A standard 75 g oral glucose tolerance test was performed at the end of each cycle and b.ood was collected and used to measure plasma glucose, insulin, c-peptide and cortisol. Fasting plasma cortisol was significantly lower after the Ginkgo b.lob. cycle than the placeb. cycle (326 ± 149 vs. 268 ± 121 nmol/L, respectively;p = 019). The plasma cortisol area under the curve during the 2-hour test (AUC0-2) was also significantly lower after ingestion of the Ginkgo b.lob. cycle compared to the placeb. (668 ± 265 vs. 530 ± 213 nmol/L/h, respectively;p b.lob. extract has effect on the hypothalamic-pituitary-adrenal axis leading to reduced b.sal cortisol levels and reduced cortisol production in response to acute hyperglycemic challenge.

关键词： Ginkgo b.lob. Extract Cortisol Acute Hyperglycemia