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First Characterization of <i>Escherichia coli</i>Strains Isolated from Wildlife Griffon Vulture (<i>Gyps fulvus</i>) in the Southeast of Spain

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Open Journal of Veterinary Medicine 2014年第12期4卷 329-333页

作者： azucena mora Nieves Ortega Elena Garcia Susana Viso Mónica G. Candela Ghizlane Dahbi Francisco Cuello María Rosa Caro Laboratorio de Referencia de E. coli (LREC) Departamento de Microbiología y Parasitología Facultad de Veterinaria Universidad de Santiago de Compostela Lugo Spain Departamento de Sanidad Animal Facultad de Veterinaria Campus de Espinardo Universidad de Murcia Murcia Spain

The aim of the present study was to characterize a collection of Escherichia coli strains isolated from asymptomatic griffon vulture (Gyps fulvus) during a reintroduction program in the southeast of Spain, in order to establish if griffon vulture could play a role in the spread of resistant or potentially pathogenic E. coli strains. For this purpose, 14 E. coli strains obtained from 10 griffon vulture were studied to establish their serotypes, phylogroups, virulencegene profiles and antimicrobial resistances. High heterogeneity was observed within the 14 strains isolated which belonged to three phylogroups (A, B1 and D), 8 serogroups (O2, O21, O29, O60, 073, O78, O103 and O141) and 13 different serotypes. Out of 34 genes screened, we have detected eight virulence genes that are typical of extraintestinal pathogenic E. coli (ExPEC) (fimH, fimAvMT78, iroN, iucD, cvaC, iss, traT and tsh);however, none of the studied strains showed the ExPEC status. The 14 strains were also analyzed for the production of extended-spectrum beta-lactamases (ESBLs) and for antimicrobial resistances. None of the 14 strains were ESBL-producing E. coli, but high resistance-prevalences to ampicillin and cotrimoxazole were detected. To our knowledge, this is the first characterization of E. coli strains isolated from griffon vulture and although they did not show high virulencegene scores, they showed cotrimoxazole resistance.

关键词： Antibiotic Resistance APEC Escherichia coli ExPEC Griffon Vulture

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Effects of "Candidatus Liberibacter solanacearum"(haplotype B)on Bactericera cockerelli fitness and vitellogenesis

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Insect Science 2020年第1期27卷 58-68页

作者： Angelica Albuquerque Tomilhero Frias Freddy Ibanez azucena Mendoza William Mario de Carvalho Nunes Cecilia Tamborindeguy Department of Entomology Texas A&M University412 Heep CenterCollege StationTexasUSA DEM-Depto.de Agronomia Nucleo de Pesquisa em Biotecnologia AplicadaMaringaBrazil

"Candidatus Liberibacter solanacearum"(Lso)are phloem-restricted and unculturable Gram-negative *** five haplotypes have been identified worldwide;but only haplotypes A and B are associated with the vector Bactericera cockerelli(Sulc.)in the *** studies showed that Lso-infection reduces *** reproductive output and that Lso haplotype B is more pathogenic than Lso haplotype *** understand the interaction of Lso haplotype B and ***,the fitness of Lso-free and Lso B-infected insects,and the expression of vitellogenin(5cKg7-like),a gene involved directly in the insect reproduction were *** differences in the number of eggs oviposited,and the total number of progeny nymphs and adults were found among crosses of insects with or without *** differences in sex proportions were found between Lso B-infected and Lso-free crosses:a higher proportion of Fi adult females were obtained from Lso B-infected mothers.A significant reduction of BcVgl-like was observed in crosses performed with Lso B-infected females compared to the Lso-free *** female cohorts of different age,a significant reduction of BcVgl-like expression was measured in 7-d-old Lso B-infected females(virgin and mated)compared with 7-d-old Lso-free females(virgin and mated),*** reduction of BcVgl-like transcript was associated with a lower number of developing oocytes observed in female5s reproductive ***,this study represents the first step to understand the interaction of Lso B with ***,highlighting the effect of Lso B infection on egg production,BcVgl-like expression,and oocyte development.

关键词： feminization insect-pathogen interaction oviposition psyllids reproduction vitellogenesis

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Cyclone as a Sugar Cane Bagasse Dryer

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Chinese Journal of Chemical Engineering 2004年第6期12卷 826-830页

作者： Jefferson Luiz Gomes Correa Daniel Rezende Graminho Maria Aparecida Silva Silvia azucena Nebra Faculty of Mechanical Engineering State University of Campinas P.O. Box 6122 Campinas/SP 13083-970 BrazilFaculty of Mechanical Engineering State University of Campinas P.O. Box 6122 Campinas/SP 13083-970 BrazilFaculty of Chemical Engineering State University of Campinas P.O. Box 6066 Campinas/SP 13083-970 BrazilFaculty of Mechanical Engineering State University of Campinas P.O. Box 6122 Campinas/SP 13083-970 Brazil

Drying of sugar cane bagasse was theoretically and experimentally studied ina cyclone. The experiments were carried out using hot air as drying agent. The influence of thecyclone conical part was studied. It was shown that the conical part has a great influence on theparticle residence time and, consequently, on moisture reduction. Experimental results were alikeindustrial ones. CFX 4.4 from AEA Technology was used to simulate some experiments. Simulated andexperimental results were close and showed that the presented model leads to a good prediction.

关键词： cyclone cyclonic dryer sugar cane bagasse particle residence time dryer energy

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Fluorescence as a Tool to Study Lipid-Protein Interactions: The Case of α-Synuclein

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Open Journal of Biophysics 2013年第1期3卷 112-119页

作者： azucena Gonzalez-Horta Brenda Gonzalez Hernandez Abelardo Chavez-Montes Faculty of Biology University Autonoma de Nuevo Leon Mexico

During the past 20 years there has been a remarkable growth in the use of fluorescence in the biological sciences. Fluorescence is now a dominant methodology used extensively in biochemistry, biophysics, biotechnology, medical diagnostics, flow cytometry, DNA sequencing and genetic analysis to name a few. It is one of the most powerful methods to study protein folding, dynamics, assembly and interactions as well as membrane structure. α-Synuclein belongs to the class of intrinsically disordered proteins lacking of a well-folded structure under physiological conditions. The conversion of α-synuclein from a soluble monomer to an insoluble fibril may underlie the neurodegeneration associated with Parkinson’s disease (PD). Although the exact mechanism of α-synuclein toxicity is still unknown, it has been proposed that disturbs membrane structure, leading to increased membrane permeability and eventual cell death. This review highlights the significant role played by fluorescence techniques in unraveling the nature of interactions between α-synuclein and membranes and its implications in PD.

关键词： Fluorescence Spectroscopy Lipid-Protein Interactions α-Synuclein

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Bats and their vital ecosystem services:a global review

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Integrative Zoology 2022年第1期17卷 2-23页

作者： Leidy azucena RAMÍREZ-FRÁNCEL Leidy Viviana GARCÍA-HERRERA Sergio LOSADA-PRADO Gladys REINOSO-FLÓREZ Alfonso SÁNCHEZ-HERNÁNDEZ Sergio ESTRADA-VILLEGAS Burton KLIM Giovany GUEVARA Programa de Doctorado en Ciencias Biológicas&Grupo de Investigación en Zoología(GIZ) Facultad de CienciasUniversidad del TolimaIbaguéColombia Programa para la Conservación de los Murciélagos de Colombia BogotáD.C.Colombia Departamento de Biología&Grupo de Investigación en Zoología(GIZ) Facultad de CienciasUniversidad del TolimaIbaguéColombia Departamento de Matemáticas y Estadística Facultad de CienciasUniversidad del TolimaIbaguéColombia Yale School of the Environment Yale UniversityNew HavenConnecticutUSA Smithsonian Tropical Research Institute BalboaRepublic of Panama Department of Natural History Royal Ontario MuseumTorontoOntarioCanada

Bats play crucial ecosystem services as seed dispersers,pollinators,controllers of insects,and nutrient ***,there has not been a thorough global review evaluating these roles in bats across all biogeographical regions of the *** reviewed the literature published during the last two decades and identified 283 relevant studies:78 dealt with the control of potential insect pests by bats,80 related to the suppression of other arthropods,60 on the dispersal of native or endemic seeds,11 dealt with the dispersal of seeds of introduced plants,29 on the pollination of native or endemic plants,1 study on pollination of introduced plants,and 24 on the use of guano as *** literature search showed that queries combining the terms“seed dispersal,”“insectivorous bats,”“nectarivorous bats,”“use of guano,”and“ecosystem services”returned 577 studies,but half were experimental in *** found that the evaluation of ecosystem services by bats has been mostly conducted in the Neotropical and Palearctic *** detect differences across relevant studies,and to explain trends in the study of ecosystem services provided by bats,we performed generalized linear mixed models(GLMM)fitted with a Poisson distribution to analyze potential differences among sampling *** identified 409 bat species that provide ecosystem services,752 insect species consumed by bats and 549 plant species either dispersed or pollinated by *** review summarizes the importance of conserving bat populations and the ecological services they provide,which is especially important during the current pandemic.

关键词： chiroptera ecosystem services frugivory,guano,insectivory nectarivory pest control pollination seed dispersal

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怎么就没信号了呢

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初中生学习(高) 2012年第12期 10-11页

作者： azucena 不详

你坐着火车出了城,打着电话聊着天儿,吃着火锅唱着歌儿。突然,手机没信号了!面对突然掉线的手机,和丝毫没有复苏迹象的信号格数,先别忙着抓狂,让我们先来搞清楚你是怎么把信号弄丢的吧!信号争夺战若手机也有眼睛,那么在它看来,这个世界... 详细信息

你坐着火车出了城,打着电话聊着天儿,吃着火锅唱着歌儿。突然,手机没信号了!面对突然掉线的手机,和丝毫没有复苏迹象的信号格数,先别忙着抓狂,让我们先来搞清楚你是怎么把信号弄丢的吧!信号争夺战若手机也有眼睛,那么在它看来,这个世界就是一张巨大的网格,其上分布着各个手机信号发射塔,有的很显眼,有的则藏得比较深。这些发射塔都有一定的覆盖范围。这种范围一般呈圆形,在开阔的乡野地带,其半径可达几千米,而在城市则往往只有不到500米。覆盖区域之间有时会有重叠,这样无论你走到哪里都不至于打不出电话。

关键词：发射塔覆盖范围

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怎么就没信号了呢

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天天爱学习（四年级） 2014年第19期 28-29页

作者： azucena

你坐着火车出了城，打着电话聊着天儿，吃着火锅唱着歌儿.突然，手机没信号了!面对突然掉线的手机，和丝毫没有复苏迹象的信号格数，别·陋着抓狂，让我们先来搞清楚你是怎么把信号弄丢的吧!信号争夺战若手机也有眼睛，那么在它看来，... 详细信息

你坐着火车出了城，打着电话聊着天儿，吃着火锅唱着歌儿.突然，手机没信号了!面对突然掉线的手机，和丝毫没有复苏迹象的信号格数，别·陋着抓狂，让我们先来搞清楚你是怎么把信号弄丢的吧!信号争夺战若手机也有眼睛，那么在它看来，这个世界就是一张巨大的网格，其上分布着各个手机信号发射塔，有的很显眼，有的则藏得比较深。

关键词：手机信号争夺战电话

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Endoscopic response to tumor necrosis factor inhibitors predicts long term benefits in Crohn's disease

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World Journal of Gastroenterology 2019年第14期25卷 1764-1774页

作者： Ignacio Alfaro Maria Carme Masamunt Nuria Planell Alicia López-García Jesús Castro Marta Gallego Rebeca Barastegui Angel Giner Alejandro Vara azucena Salas Elena Ricart Julián Panés Ingrid Ordás Gastroenterology Department Hospital Clínic de Barcelona Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) University of Barcelona

BACKGROUND Identifying predictors of therapeutic response is the cornerstone of personalized *** To identify predictors of long-term mucosal healing(MH) in patients with Crohn's disease(CD) treated with tumor necrosis factor α(TNF-α) *** Prospective single center study. Consecutive patients with clinically active CD requiring treatment with a TNF-α inhibitor were included. A baseline segmental CD Endoscopic Index of Severity(CDEIS) ≥ 10 in at least one segment or the presence of ulcerations were required for inclusion. Clinical, biological and endoscopic data were obtained at baseline, weeks 14 and 46. Endoscopic response(ER) was defined as a decrease ≥ 50% from baseline CDEIS and MH as partial CDEIS ≤ 5 in all *** Of 62 patients were included. At baseline, median CD Activity Index and CDEIS were 201 and 6.7, respectively with a significant reduction after one year of treatment(53 and 3.0 respectively, P < 0.001). At week 14, 56% of patients achieved ER and 34% MH. At week 46, the corresponding percentages were 52%and 44%. Baseline disease characteristics or biomarkers did not predict MH. A decrease from baseline CDEIS at week 14 of at least 80% was the best predictor of MH at week 46(59% sensitivity and 91% specificity; area under the curve =0.778).CONCLUSION Clinical and biomarker data are not useful predictors of response to TNF-αinhibitors in CD, whereas ER to induction therapy, defined as 80% reduction in global CDEIS, is a robust predictor of long-term MH. Achievement of this endoscopic endpoint may be considered as a therapeutic target for anti-TNF-αtherapy.

关键词： Crohn’s disease Endoscopy Mucosal healing Crohn’s Disease Endoscopic Index of Severity Tumor necrosis factor

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Association between Metabolic Syndrome and Erythrocyte Fatty Acid Profile in Mexican Adolescents: A Trans Fatty Acid Approach

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Food and Nutrition Sciences 2013年第9期4卷 51-58页

作者： Martínez-Razo Gabriel Martínez-Basila azucena Salas-Fernández Alejandra Maldonado-Hernández Jorge Mass Spectrometry Laboratory Research Unit in Medical Nutrition Pediatric Hospital National Medical Center 21st Century Mexican Institute of Social Security Mexico City Mexico.

The type of fat consumed in the Mexican diet could predispose to the development of Metabolic Syndrome (MS) which has been associated with an increased risk to develop cardiovascular disease and type 2 diabetes mellitus. Our study included adolescents between 12 and 16 years of age, divided in two groups: Control Group (n = 31) and MS Group (n = 44). Waist circumference, blood pressure, fasting glucose, triglycerides, and HDL-cholesterol were determined. Erythrocytes’ fatty acids methyl esthers were quantified using gas chromatography with ionized flame detector. We identified 16 fatty acids (FA) with chain lengths from C12 to C24, with emphasis in four trans FA (TFA) isomers: vaccenic (C18:1n7t), elaidic (C18:1n9t), linoelaidic (C18:2n6t), and conjugated linoelaidic acids (C18:2n7t). MS Group had a less proportion of: myristic (C14), palmitoleic (C16:1), C18:1n7t, and linoleic acids (C18:2);and a higher one of C18:1n9t, C18:2n7t, and nervonic acids (C24:1) when compared to the control group. C24:1 and C18:1n9t had a significant positive association with MS (OR = 14.17 and OR = 12.94, respectively);whereas C14 (OR = 0.14), C18:1n7t (OR = 0.14), and C18:2 (OR = 0.22) appear to have a protective effect against the disease. The proportion of specific FAs in erythrocytes’ membranes differs between adolescents with MS and healthy controls;these FA not only showed a strong association with MS, but also correlated with most of its individual components. Interestingly, TFA displayed an antagonic behavior;while C18:1n9t had a strong association with MS, apparently C18:1n7t confers a protective effect;these results suggest that analyzing each TFA separately will constitute a more accurate approach to determine the role of TFAs in the pathogenesis of MS or other related metabolic disorders.

关键词： Metabolic Syndrome Fatty Acid Profile Erythrocyte Membrane

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Biochar from grape pomace,a waste of vitivinicultural origin,is effective for root-knot nematode control

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Biochar 2023年第1期5卷 519-541页

作者： Ángela Martínez-Gómez Maria Fe Andrés Ángel Barón-Sola Fernando E.Díaz-Manzano Ibraheem Yousef Ismael F.Mena Elena Díaz Óscar Gómez-Torres azucena González-Coloma Luis E.Hernández Carolina Escobar Facultad de Ciencias Ambientales y Bioquímica Universidad de Castilla-La ManchaToledoSpain Departamento de Protección Vegetal Instituto Ciencias Agrarias CSICMadridSpain Departamento de Biología Universidad Autónoma de MadridMadridSpain CELLS ALBA Carrer del Llum 2-2608290Cerdanyola del VallèsBarcelonaSpain Departamento de Ingeniería Química Universidad Autónoma de MadridMadridSpain Faculty of Chemical Sciences&Technologies Chemical Engineering DepartmentAv.Camilo JoséCela1213071University of Castilla-La ManchaCiudad RealSpain

Root-knot nematodes(RKNs)are obligate endoparasites that feed on their host plants to complete its life cycle,representing a major threat to agriculture and economy *** development of new management strategies becomes essential as effective chemical nematicides are progressively being ***,we analysed grape pomace-derived biochars,pyrolysed at 350℃(BC350)and 700℃(BC700),focusing on their potential for RKN *** thermal treatment of grape pomace caused an increase in the concentration of carbon and plant macro-and micronutrients,which were largely present in a water-soluble *** radiation-based Fourier transform infrared microspectroscopy data showed a general loss of carboxylic functional groups during pyrolysis,partially contributing to the alkalinisation of both biochars,mostly in *** electron microscopy coupled with energydispersive X-ray spectroscopy analysis revealed a highly porous structure filled with different crystals composed of elements such as K,Ca,Mg,P,Si or Al,which could be a suitable environment for the growth of ***-derived aqueous extracts showed phytotoxicity to tomato seedlings at high concentrations,and disappeared upon dilution,but no toxic effect was observed on the nematode’s infective ***,the infective and reproductive traits of a Meloidogyne javanica population in tomato were significantly reduced(*** masses and eggs per plant)in washed-biochar-treated soil in pots(0.75%;BC350W).Therefore,the large amount of grape waste generated after wine production can be transformed into a valuable product such as biochar,effective for RKNs control,thus reducing the waste management problem and contributing to a circular economy.

关键词： Biochar Grape pomace Tomato Root-knot nematode control Meloidogyne spp.

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