检索结果-南通市图书馆

Different Thermostability of Skeletal Muscle Glyceraldehyde-3-phosphate Dehydrogenase from Hibernating and Euthermic Jerboa (Jaculus orientalis)

引用

Acta Biochimica et Biophysica Sinica 2003年第10期35卷 891-896页

作者： IDDAR Abdelghani,CAMPOS Luis A.,SANCHO Javier,SERRANO aurelio,SOUKRI Abdelaziz Laboratoire de Biochimie Departamento de Bioquímica y Biología Molecular y Celular Departamento de Bioquímica y Biología Molecular y Celular Instituto de Bioquímica Vegetal y Fotosíntesis Laboratoire de Biochimie Unitè de Gènie Enzymatique et Biologie Molèculaire Facultè des Sciences-Ain Chock Casablanca Morocco Universidad de Zaragoza Zaragoza Spain Universidad de Zaragoza Zaragoza Spain CSIC-Universidad de Sevilla Sevilla Spain Unitè de Gènie Enzymatique et Biologie Molèculaire Facultè des Sciences-Ain Chock Casablanca Morocco

In previous study, we demonstrated that the specific activity of D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.***.1.12) in skeletal muscle of induced hibernating jerboa (hibernating GAPDH) was 3-4 folds lower than that of the one in the skeletal muscle of the euthermic jerboa (euthermic GAPDH). A significant decrease in both GAPDH protein and GapC mRNA levels occurs when hibernating, but the purified hibernating GAPDH is less active than the euthermic GAPDH. To investigate the physico-chemical basis of this lower activity, the behaviour during thermal inactivation of skeletal muscle GAPDH from hibernating and euthermic tissues was examined by a variety of spectroscopic techniques, including fluorescence emission, circular dichroism and ultraviolet absorption. A clear resistance to thermal denaturation was observed in the hibernating GAPDH compared with the euthermic GAPDH. The different temperature of denaturation found in these proteins by both fluorimetry and circular dichroism indicates that there might exist conformational changes of GAPDH upon hibernation that could affect the stability of this enzyme.

关键词： glyceraldehyde-3-phosphate dehydrogenase hibernation thermal denaturation circular dichroism spectroscopy protein stability

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Total plasma homocysteine and methylenetetrahydrofolate reductase C677T polymorphism in patients with colorectal carcinoma

引用

World Journal of Gastroenterology 2006年第38期12卷 6128-6132页

作者： Sandra Battistelli aurelio Vittoria Massimo Stefanoni Camilla Bing Franco Roviello Dipartimento di Chirurgia e Specialità Chirurgiche Università degli Studi Policlinico Le Scotte Viale Bracci Siena 53100 Italy Dipartimento di Medicina Clinica e Scienze Immunologiche Università degli Studi Policlinico Le Scotte Viale Bracci Siena 53100 Italy Dipartimento di Medicina e Chirurgia d’ Urgenza e di Accettazione Ospedale Civile di Gorizia Italy Dipartimento di Patologia Umana ed Oncologia U.O. Chirurgia Oncologica Università degli Studi Policlinico Le Scotte Viale Bracci Siena 53100 Italy

AIM: To investigate the behaviour of total plasma homocysteine (tHcy) and its most common genetic determinant defect, the methylenetetrahydrofolate reductase C677T (C677TMTHFR) polymorphism in patients with early stage colorectal carcinoma. METHODS: tHcy was quantified by Abbott IMx immunoassay; screening for C677TMTHFR substitution was performed by PCR and restriction analysis. RESULTS: The frequency of the C/T and T/T genotypes of the C677TMTHFR gene polymorphism did not differ between the groups. The mean tHcy was statistically higher in cancer patients than in control subjects carrying the same C/C or C/T genotype, whereas there was no difference in the T/T homozygous carriers of the two groups. tHcy was significantly higher in the T/T homozygous carriers than in C/C and C/T genotype carriers. CONCLUSION: The statistically significant increase of tHcy observed in C/C and C/T genotype carriers among our cancer patients is related to substrate consumption dependent on the tumor cell proliferation rate, whereas the tHcy increase observed in T/T genotype carriers of both groups probably depends on the enzymatic deficit of the homocysteine conversion to methionine and/or on the folate deficiency.

关键词： Homocysteine Colorectal cancer Methylenet etrahydrofolate reductase C677T polymorphism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Core-softened potentials, multiple liquid-liquid critical points, and density anomaly regions： An exact solution

引用

Frontiers of physics 2018年第1期13卷 85-94页

作者： Eduardo O. Rizzatti Marco aurelio A. Barbosa Marcia C. Barbosa Instituto de Fisica Universidade Federal do Rio Grande do Sul Porto Alegre-RS Brazil Programa de Pos-Graduacao em Ciencia de Materiais Universidade de Brasilia Planaltina-DF Brazil

The pressure versus temperature phase diagram of a shoulder-like potential is exactly computed in one system of particles interacting through a multiscale dimension. The N-shoulder potential exhibits N density anomaly regions in the phase diagram if the length scales can be connected by a convex curve The result is analyzed in terms of the convexity of the Gibbs free energy.

关键词： density anomalies regions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comprehensive control for unified power quality conditioners

引用

Journal of Modern Power Systems and Clean Energy 2017年第4期5卷 609-619页

作者： Miguel OCHOA-GIMéNEZ aurelio GARCíA-CERRADA Juan Luis ZAMORA-MACHO Gamesa Electric S.A. 28823 CosladaMadridSpain School of Engineering Comillas Pontifical University28015 MadridSpain

This paper presents a comprehensive control strategy for unified power quality conditioners(UPQCs) to compensate for both voltage and current quality *** controllers for the series and shunt components of the UPQC are, equally, divided into three blocks: à main controller, which deals with the fundamental-frequency issues such as active and reactive power flow;` harmonic controller, which ensures zero-error tracking while compensating voltage and current harmonics;′ the set-point generation block, which handles the different control objectives of the UPQC. The controller design procedure has been simplified to the selection of three parameters for each converter. Furthermore, the proposed strategy can be implemented measuring only four variables, which represents a reasonable number of sensors. In addition, a pulse width modulation(PWM)-based modulation with fixed switching frequency is used for both converters. The proposed control strategy has been validated experimentally under different conditions, including grid-frequency variations.

关键词： Unified power quality conditioner(UPQC) Powerquality Harmoniccontrol Pulse width modulation(PWM)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adalimumab in ulcerative colitis: Two cases of mucosal healing and clinical response at two years

引用

World Journal of Gastroenterology 2009年第30期15卷 3814-3816页

作者： Manuel Barreiro-de Acosta aurelio Lorenzo Juan Enrique Dominguez-Muoz Department of Gastroenterology University Hospital of Santiago de CompostelaC/Choupanas/nE-15706-Santiago de CompostelaSpain

Infliximab (IFX) is currently the only biologic therapy used in the treatment of moderate-to-severe ulcerative colitis (UC). In the years to come, more biologic therapies will have a role in the management of moderate-to-severe UC. We report on two patients with steroid-dependent UC who, due to adverse reactions to IFX, have been under therapy with adalimumab for two years. Both patients received concomitant immunosuppressive treatment. Long term clinical remission and mucosal healing are described.

关键词： Adalimumab Ulcerative colitis Mucosalhealing Maintenance treatment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcriptome Analysis Provides Novel Insights into Salt Stress Response in Two Egyptian Rice Varieties with Different Tolerance Levels

引用

Rice science 2022年第6期29卷 499-502页

作者： Shehab MOHAMED Iovene MARINA Ciancio aurelio Colagiero MARIANTONIETTA Finetti-Sialer MARIELLA Rice Research and Training Center Field Crops Research InstituteAgriculture Research CenterSakha Kafr El-Sheikh 33717Egypt Institute of Biosciences and Bioresources National Research CouncilBari 70126Italy Sustainable Plant Protection Institute National Research CouncilBari 70126Italy

The response of rice to salt stress(200 mmol/L NaCl)was investigated at the transcription level in Egyptian varieties Giza 177(salt sensitive variety)and Giza 178(salt tolerant variety).We applied a genome-wide RNA-Se... 详细信息

关键词： varieties Egyptian stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prevalence of factor V Leiden and prothrombin G20210A in patients with gastric cancer

引用

World Journal of Gastroenterology 2006年第26期12卷 4179-4180页

作者： Sandra Battistelli Massimo Stefanoni Alberto Genovese aurelio Vittoria Roberto Cappelli Franco Roviello Dipartimento di Chirurgia e Specialità Chimrgiche Policlinico Le Scotte Viale Bracci53100 SienaItaly Dipartimento di Medicina Clinica e Scienze Immunologiche Policlinico Le Scotte Viale Bracci53100 SienaItaly Dipartimento di Medicina Interna Cardiovascolare e Geriatrica Policlinico Le ScotteViale Bracci53100 SienaItaly Dipartimento di Patologia Umana ed Oncologia Policlinico Le Scotte Viale Bracci53100 SienaItaly

AIM： To analyze the prevalence of the two commonest thrombophilic mutations, factor V Leiden and prothrombin G20210A, in patients with gastric cancer. METHODS： One hundred and twenty-one patients with primary gastric carcinoma and 130 healthy subjects, comparable for age and sex, were investigated. Factor V Leiden was detected by using polymerase chain reaction and restriction enzyme digestion, and prothrombin G20210A gene mutation by allele-specific PCR. RESULTS： Among the 121 cancer patients, factor V Leiden was found in 4 cases （GA genotype： 3.3%） and prothrombin G20210A in 10 cases （GA genotype： 8.3%）. Of the 130 control subjects, factor V Leiden was detected in 6 cases （GA genotype： 4.6%） and prothrombin G20210A in 8 cases （GA genotype： 6.1%）. No double heterozygous carriers of both mutations were found in either group. The prevalence of both factor V Leiden and prothrombin G20210A variant was not statistically different between the cancer patients and the healthy subjects. CONCLUSION： Our study suggests that, in gastric cancer, the risk factors of thrombophilic cancer state are on acquired rather than on a genetic basis and that prothrombin G20210A does not seem to be a cofactor in gastric cancer pathogenesis.

关键词： Gastric cancer Genetic polymorphism Factor V Leiden Prothrombin G20210A

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Influence of obesity and bariatric surgery on gastric cancer

引用

Cancer Biology & Medicine 2016年第2期13卷 269-276页

作者： Anna Carolina Batista Dantas Marco aurelio Santo Roberto de Cleva Rubens Antonio Aissar Sallum Ivan Cecconello Department of Digestive Surgery University of Sao Paulo School of Medicine Sao Paulo-SP 05403-000 Brazil

Esophageal and gastric cancer(GC) are related to obesity and bariatric surgery. Risk factors, such as gastroesophageal reflux and Helicobacter pylori, must be investigated and treated in obese population. After surgery, GC reports are anecdotal and treatment is not standardized. This review aims to discuss GC related to obesity before and after bariatric surgery.

关键词： Obesity bariatric surgery stomach neoplasms esophageal neoplasms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Percutaneous electrochemotherapy in the treatment of portal vein tumor thrombosis at hepatic hilum in patients with hepatocellular carcinoma in cirrhosis: A feasibility study

引用

World Journal of Gastroenterology 2017年第5期23卷 906-918页

作者： Luciano Tarantino Giuseppina Busto aurelio Nasto Raffaele Fristachi Luigi Cacace Maria Talamo Catello Accardo Sara Bortone Paolo Gallo Paolo Tarantino Riccardo aurelio Nasto Matteo Nicola Dario Di Minno Pasquale Ambrosino Department of Surgery Interventional Hepatology UnitAndrea Tortora Hospital Department of Oncology Andrea Tortora Hospital Department of Surgery Unit of General Surgery and OncologyAndrea Tortora Hospital Department of Pathology Andrea Tortora Hospital Department of Radiology Andrea Tortora Hospital Department of Advanced Biomedical Sciences Division of CardiologyFederico II University Unit of Cell and Molecular Biology in Cardiovascular Diseases Centro Cardiologico MonzinoIRCCS Department of Clinical Medicine and Surgery Federico II University

AIMTo treated with electrochemotherapy (ECT) a prospective case series of patients with liver cirrhosis and Vp3-Vp4- portal vein tumor thrombus (PVTT) from hepatocellular carcinoma (HCC), in order to evaluate the feasibility, safety and efficacy of this new non thermal ablative technique in those *** patients (5 males and 1 female), aged 61-85 years (mean age, 70 years), four in Child-Pugh A and two in Child-Pugh B class, entered our study series. All patients were studied with three-phase computed tomography (CT), contrast enhanced ultrasound (CEUS) and ultrasound-guided percutaneous biopsy of the thrombus before ECT. All patients underwent ECT treatment (Cliniporator Vitae®, IGEA SpA, Carpi, Modena, Italy) of Vp3-Vp4 PVTT in a single session. At the end of the procedure a post-treatment biopsy of the thrombus was performed. Scheduled follow-up in all patients entailed: CEUS within 24 h after treatment; triphasic contrast-enhanced CT and CEUS at 3 mo after treatment and every six months ***-treatment CEUS showed complete absence of enhancement of the treated thrombus in all cases. Post-treatment biopsy showed apoptosis and necrosis of tumor cells in all cases. The follow-up ranged from 9 to 20 mo (median, 14 mo). In 2 patients, the follow-up CT and CEUS demonstrated complete patency of the treated portal vein. Other 3 patients showed a persistent avascular non-tumoral shrinked thrombus at CEUS and CT during follow-up. No local recurrence was observed at follow-up CT and CEUS in 5/6 patients. One patient was lost to follow-up because of death from gastrointestinal hemorrage 5 wk after *** patients with cirrhosis, ECT seems effective and safe for curative treatment of Vp3-Vp4 PVTT from HCC.

关键词： Hepatocellular carcinoma Portal vein tumor thrombosis Electrochemotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

How and why chemicals from tobacco smoke can induce a rise in blood pressure

引用

World Journal of Pharmacology 2012年第1期1卷 10-20页

作者： aurelio Leone Department of Internal Medicine City Hospital Massa 19030 Castelnuovo Magra (SP) Italy the Royal Society for Promotion of Health (FR-SPH) London SW1V 4BH United Kingdom

The primary objective of this article is to analyze the role of tobacco smoke compounds able to damage the cardiovascular system and, in particular, to interfere with blood pressure. They are products of tobacco plant leaves, like nicotine, thiocyanate and aromatic amines, and a chemical derived from cigarette com-bustion, carbon monoxide. Of the other thousands of chemicals, there is no clear evidence of cardiovascular damage. Nicotine and its major metabolite, cotinine, usually increase blood pressure by a direct action and an action stimulating neuro-humoral metabolites of the body as well as sympathetic stimulation. An indirect mechanism of damage exerted by elevated carboxyhe-moglobin concentrations is mediated by carbon mon-oxide, which, mainly induces arterial wall damage and, consequently, late rising in blood pressure by a toxic direct action on endothelial and blood cells. Thiocya-nate, in turn, reinforces the hypoxic effects determined by carbon monoxide. Aromatic amines, depending on their chemical structure, may exert toxic effects on the cardiovascular system although they have little effect on blood pressure. A rise in blood pressure determined by smoking compounds is a consequence of both their direct toxicity and the characteristics of their chemical chains that are strongly reactive with a large number of molecules for their spatial shape. In addition, a rise in blood pressure has been documented in individuals smoking a cigarette, acutely and chronically, with irre-versible artery wall alterations several years after begin-ning smoking. Since cigarette smoking has a worldwide diffusion, the evidence of this topic meets the interest of both the scientifc community and those individuals aiming to control smoking.

关键词： Smoking chemicals Blood pressure Nicotine Carbon monoxide Arterial damage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：