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Interleukin-12 as a Genetic Adjuvant Enhances Hepatitis C Virus NS3 DNA Vaccine Immunogenicity

Virologica Sinica 2013年第3期28卷 167-173页

作者： Malihe Naderi atefeh saeedi Abdolvahab Moradi Mishar Kleshadi Mohammad Reza Zolfaghari Ali Gorji Amir Ghaemi Department of Microbiology Qom branch Islamic Azad University Department of Microbiology Lahijan Branch Islamic Azad University Golestan Research Center of Gastroenterology and Hepatology-GRCGH Department of Microbiology Golestan University of Medical Sciences Shefa Neuroscience Research Centre Institut für Physiologie I Westflische Wilhelms-Universitt Münster Klinik und Poliklinik für Neurochirurgie Westf lische Wilhelms-Universitt Münster

Hepatitis C virus (HCV) chronic infection is a worldwide health problem, and numerous efforts have been invested to develop novel vaccines. An efficient vaccine requires broad immune response induction against viral proteins. To achieve this goal, we constructed a DNA vaccine expressing nonstructural 3 (NS3) gene (pcDNA3.1-HCV-NS3) and assessed the immune response in C57BL/6 mice. In this study, the NS3 gene was amplified with a nested-reverse transcriptase-polymerase chain reaction (RT-PCR) method using sera of HCV-infected patients with genotype 1a. The resulting NS3 gene was subcloned into a pcDNA3.1 eukaryotic expression vector, and gene expression was detected by western blot. The resultant DNA vaccine was co-administered with interleukin-12 (IL-12) as an adjuvant to female C57BL/6 mice. After the final immunizations, lymphocyte proliferation, cytotoxicity, and cytokine levels were assessed to measure immune responses. Our data suggest that co-administration of HCV NS3 DNA vaccine with IL-12 induces production of significant levels of both IL-4 and interferon (IFN)-γ (p<0.05). Cytotoxicity and lymphocyte proliferation responses of vaccinated mice were significantly increased compared to control (p<0.05). Collectively, our results demonstrated that co-administration of HCV NS3 and IL-12 displayed strong immunogenicity in a murine model.

关键词： Hepatitis C virus (HCV) NS3 Interleukin-12 DNA vaccine

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From furnace up to freezer:Elevational patterns of plant diversity in Mount Palvar,a semi-arid Irano-Turanian mountain range of southwest Asia

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Journal of Mountain Science 2024年第7期21卷 2236-2248页

作者： atefeh GHORBANALIZADEH Moslem DOOSTMOHAMMADI Kerman *** Iran

Much of the world's biodiversity lies in heterogeneous mountain areas with their diverse *** an example,Iranian montane ranges are highly diverse,particularly in the Irano-Turanian phytogeographical *** plant diversity patterns with increasing elevation is of high significance,not least for conservation *** studied the pattern of species richness,Shannon diversity,endemic richness,endemics ratio,and richness of life forms along a 3900 m elevational transect in Mount Palvar,overlooking the Lut Desert in Southeast *** also analyzed the effect of environmental variables on species turnover along the vertical gradient.A total of 120 vegetation plots(10 m×10 m)were sampled along the elevational transect containing species and environmental *** discover plant diversity pattern along the elevational gradient,generalized additive model(GAM)was ***-metric multidimensional scaling(NMDS)was applied for illustrating the correlation between species composition and environmental *** found hump-shaped pattern for species richness,Shannon diversity,endemic richness,and species richness of different life forms,but a monotonic increasing pattern for ratio of endemic species from low to high *** study confirms the humped pattern of species richness peaking at intermediate elevations along a complete elevational gradient in a semi-arid *** monotonic increase of endemics ratio with elevation in our area as a case study is consistent with global increase of endemism with *** to our results,temperature and precipitation are two important climatic variables that drive elevational plant diversity,particularly in seasonally dry *** study suggests that effective conservation and management are needed for this low latitude mountain area along with calling for long-term monitoring for species redistribution.

关键词： Elevational gradient Biodiversity Endemic Generalized additive model Hump-shaped pattern Irano-Turanian region

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The potent osteo-inductive capacity of bioinspired brown seaweed-derived carbohydrate nanofibrous three-dimensional scaffolds

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Marine Life Science & Technology 2024年第3期6卷 515-534页

作者： Zahra Dadashi Ouranj Saadi Hosseini atefeh Alipour Shahin Homaeigohar Shahram Azari Leila Ghazizadeh Mohammadali Shokrgozar Sabu Thomas Saeed Irian Hosein Shahsavarani Department of Cell and Molecular Biology Faculty of Biological SciencesKharazmi UniversityTehran 15719-14911Iran Laboratory of Regenerative Medicine and Biomedical Innovations National Cell Bank of IranPasteur Institute of IranTehran 13169-43551Iran Department of Nanobiotechnology Pasteur Institute of IranTehran 13169-43551Iran School of Science and Engineering University of DundeeDundee DD14HNUK School of Chemical Sciences Mahatma Gandhi UniversityKottayamKeralaIndia Department of Cell and Molecular Biology Faculty of Life Sciences and BiotechnologyShahid Beheshti UniversityTehran 19839-69411Iran Iranian Biological Resource Center Academic Center for EducationCulture and Research(ACECR)Tehran ***Iran

This study aimed to investigate the osteo-inductive capacity of a fucoidan polysaccharide network derived from brown algae on human adipose-derived stem cells(HA-MSCs)for bone *** physiochemical properties of the scaffold including surface morphology,surface chemistry,hydrophilicity,mechanical stiffness,and porosity were thoroughly *** in vitro and in vivo measurements implied a superior cell viability,proliferation,adhesion,and osteo-inductive performance of obtained scaffolds compared to using specific osteogenic induction medium with increased irregular growth of calcium crystallites,which mimic the structure of natural *** scaffold was highly biocompatible and suitable for cell *** examinations,such as quantification of mineralization,alkaline phosphatase,gene expression,and immunocytochemical staining of pre-osteocyte and bone markers confirmed that HAD-MSCs differentiate into osteoblasts,even without an osteogenic induction *** study provides evidence for the positive relationship and synergistic effects between the physical properties of the decellularized seaweed scaffold and the chemical composition of fucoidan in promoting the osteogenic differentiation of ***,the natural matrices derived from brown seaweed offers a sustainable,cost-effective,non-toxic bioinspired scaffold and holds promise for future clinical applications in orthopedics.

关键词： Bone tissue engineering Seaweed-derived scaffold Fucoidans HA-MSCs differentiation Osteoblasts

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Resolution of sperm quality impairment following SARS-CoV-2 infection:A prospective study

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Asian pacific Journal of Reproduction 2024年第5期13卷 207-212页

作者： Marzieh Derakhshan Maryam Derakhshan Elham Naghshineh Minoo Movahedi Hatav Ghasemi-Tehrani Fatemeh Bamarinejad atefeh Bamarinejad Zeinab Omidvar Department of Obstetrics and Gynecology/Anatomy School of MedicineIsfahan University of Medical SciencesIsfahanIran Shahid Beheshti Fertility Center Isfahan University of Medical SciencesIsfahanIran Department of Pathology School of MedicineIsfahan University of Medical SciencesIsfahanIran School of Medicine Isfahan University of Medical SciencesIsfahanIran

Objective:To investigate the length of time required to resolve COVID-19 effects on semen quality and DNA ***:A prospective cohort study was conducted among 42 men who tested positive for SARS-CoV-2 and underwent semen analysis at baseline and four months’*** samples were collected and evaluated for macroscopic and microscopic parameters,sperm chromatin maturation,and DNA ***:The mean age of participants was 37(±7)years,and 14%had normozoospermia at *** a four-month recovery from COVID-19,48%of patients had *** count,motility,and morphology increased significantly,while sperm DNA fragmentation and sperm chromatin maturation decreased significantly post-recovery from ***:Sperm parameters improve after a four-month recovery from *** findings indicate significant improvements in sperm count,motility,morphology,DNA fragmentation,and chromatin maturation after a four-month recovery period.

关键词： SARS-CoV-2 Male fertility Recovery Sperm DNA fragmentation Sperm parameter

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MOS structure fabrication by thermal oxidation of multilayer metal thin films

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Journal of Semiconductors 2011年第3期32卷 135-139页

作者： Mohammad Orvatinia atefeh Chahkoutahi Electron Engineering Department Information and Communication Technology(ICT) FacultyICT Ministry of Iran Bushehr Azad University

A novel approach for the fabrication of a metal oxide semiconductor（MOS） structure was *** process comprises electrochemical deposition of aluminum and zinc layers on a base of nickel-chromium alloy. This two-layer structure was thermally oxidized at 400℃for 40 min to produce thin layers of aluminum oxide as an insulator and zinc oxide as a semiconductor on a metallic *** deposition parameters,device dimensions and SEM micrographs of the layers,the device parameters were *** resultant MOS structure was characterized by a C-V curve *** this curve,the device maximum capacitance and threshold voltage were estimated to be about 0.74 nF and -2.9 V,respectively,which are in the order of model-based calculations.

关键词： MOS structure electrochemical deposition thermal oxidation C-V curve

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Plant diversity of Hyrcanian relict forests:An annotated checklist,chorology and threat categories of endemic and near endemic vascular plant species

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Plant Diversity 2022年第1期44卷 39-69页

作者： atefeh Ghorbanalizadeh Hossein Akhani Halophytes and C4 Plants Research Laboratory Department of Plant SciencesSchool of BiologyCollege of SciencesUniversity of TehranP.O.Box 14155-6455TehranIran

In this paper a critical annotated checklist of 256 endemic and near endemic species belonging to 152 genera and 50 families of flowering plants known from Hyrcanian relict forests is *** maps of taxa,elevational range,number of known records,chorotypes,life forms,IUCN threat categories and habitat types are also *** chorotypes are categorized into eight main patterns:1)the Omni-Hyrcanian pattern(OH),2)West Hyrcanian pattern(WH),3)Manjil-Rudbar pattern(MR),4)Central Hyrcanian pattern(CH),5)Central and East Hyrcanian pattern(CEH),6)East Hyrcanian pattern(EH),7)Alborz-Hyrcanian pattern(AH),and 8)Euxino-Hyrcanian pattern(XH).The richness and distribution maps were generated based on 5408 records gained from herbarium specimens and literature *** life form spectra show that the majority of taxa(54.7%)belong to hemicryptophytes,followed by the tuberous,bulbous and parasitic geophytes with 45 species(17.6%)and phanerophytes with 28 taxa(10.9%).The conservation status of species according to IUCN criteria indicates that 30 taxa are Critically Endangered,52 taxa Endangered,30 taxa Vulnerable,25 taxa Near Threatened and 81 taxa are of Least *** present data were not sufficient to evaluate 38 taxa that are categorized here as Data *** new combination of Leutea translucens(=Peucedanum translucens)is validated with inclusion of Peucedanum hyrcanicum as its *** disjunct occurrence of the Caucasian species Gentiana grossheimii is reported from the eastern parts of the Hyrcanian forests in Iran for the first *** conclude that(i)the Hyrcanian forests and associated habitats in the northern slopes of the Alborz Mountains harbour tremendous floristic diversity of high conservation priority,and(ii)the Hyrcanian forest zone is an important and unique center of endemism within the Euro-Siberian region that should be considered a floristic province with a large number of relict species.

关键词： Biodiversity Distribution pattern Endemic plant Hyrcanian forests IUCN category

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Phototriggered structures:Latest advances in biomedical applications

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药学学报:英文版 2023年第7期13卷 2844-2876页

作者： Mojtaba Shamsipur atefeh Ghavidast Afshin Pashabadi Department of Chemistry Razi UniversityKermanshah ***Iran

Non-invasive control of the drug molecules accessibility is a key issue in improving diagnostic and therapeutic *** studies have explored the spatiotemporal control by light as a peripheral *** drug delivery systems(PTDDSs) have received interest in the past decade among biological researchers due to their capability the control drug *** this end,a wide range of phototrigger molecular structures participated in the DDSs to serve additional efficiency and a high-conversion release of active fragments under light *** to now,several categories of PTDDSs have been extended to upgrade the performance of controlled delivery of therapeutic agents based on well-known phototrigger molecular structures like o-nitrobenzyl,coumarinyl,anthracenyl,quinolinyl,o-hydroxycinnamate and hydroxyphenacyl,where either of one endows an exclusive feature and distinct mechanistic *** review conveys the design,photochemical properties and essential mechanism of the most important phototriggered structures for the release of single and dual(similar or different) active molecules that have the ability to quickly reason of the large variety of dynamic biological phenomena for biomedical applications like photo-regulated drug release,synergistic outcomes,real-time monitoring,and biocompatibility potential.

关键词： Phototrigger Light irradiation Excited state proton transfer Drug delivery system Dynamic process Controlling chemistry

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Comparison of Spatial Interpolation Methods for Gridded Bias Removal in Surface Temperature Forecasts

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Journal of Meteorological Research 2017年第4期31卷 791-799页

作者： Seyedeh atefeh MOHAMMADI Majid AZADI Morteza RAHMANI Technology Development Institute Tehran 13445-686 Iran Atmospheric Science and Meteorological Research Center Tehran 16385-14977 Iran Faculty of Basic and Advanced Technologies in Biology University of Science and Culture Tehran 14619-68151 Iran

All numerical weather prediction（NWP） models inherently have substantial biases, especially in the forecast of near-surface weather variables. Statistical methods can be used to remove the systematic error based on historical bias data at observation stations. However, many end users of weather forecasts need bias corrected forecasts at locations that scarcely have any historical bias data. To circumvent this limitation, the bias of surface temperature forecasts on a regular grid covering Iran is removed, by using the information available at observation stations in the vicinity of any given grid point. To this end, the running mean error method is first used to correct the forecasts at observation stations, then four interpolation methods including inverse distance squared weighting with constant lapse rate（IDSW-CLR）, Kriging with constant lapse rate（Kriging-CLR）, gradient inverse distance squared with linear lapse rate（GIDS-LR）, and gradient inverse distance squared with lapse rate determined by classification and regression tree（GIDS-CART）, are employed to interpolate the bias corrected forecasts at neighboring observation stations to any given location. The results show that all four interpolation methods used do reduce the model error significantly,but Kriging-CLR has better performance than the other methods. For Kriging-CLR, root mean square error（RMSE）and mean absolute error（MAE） were decreased by 26% and 29%, respectively, as compared to the raw forecasts. It is found also, that after applying any of the proposed methods, unlike the raw forecasts, the bias corrected forecasts do not show spatial or temporal dependency.

关键词： spatial interpolation bias correction lapse rate Kriging classification and regression tree

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6G-Enabled Internet of Things:Vision,Techniques,and Open Issues

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Computer Modeling in Engineering & Sciences 2022年第12期133卷 509-556页

作者： Mehdi Hosseinzadeh atefeh Hemmati Amir Masoud Rahmani Pattern Recognition and Machine Learning Lab Gachon UniversitySeongnam13120Korea Department of Computer Engineering Science and Research BranchIslamic Azad UniversityTehran***Iran Future Technology Research Center National Yunlin University of Science and TechnologyYunlin64002Taiwan

There are changes in the development of wireless technology systems every decade.6G(sixth generation)wireless networks improve on previous generations by increasing dependability,accelerating networks,increasing available bandwidth,decreasing latency,and increasing data transmission speed to standardize communication *** purpose of this article is to comprehend the current directions in 6G studies and their relationship to the Internet of Things(IoT).Also,this paper discusses the impacts of 6G on IoT,critical requirements and trends for 6G-enabled IoT,new service classes of 6G and IoT technologies,and current 6G-enabled IoT studies selected by the systematic literature review(SLR)method published from 2018 to *** addition,we present a technical taxonomy for the classification of 6G-enabled IoT,which includes self-organization systems,energy efficiency,channel assessment,and ***,according to the articles reviewed,we consider the evaluation factors in this domain,including data transmission,delay,energy consumption,and ***,we focus on open issues and future research challenges in 6G-enabled *** mention important future challenges and directions,we can point to migration,data storage,data resource,data security,data sharing,data offloading,availability,scalability,portability,user experience,reliability,authentication,and authorization.

关键词： Internet of Things 6G wireless network machine learning artificial intelligence

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Inhibitability of soil loss and sediment concentration during consecutive rainfalls from experimental plots treated by endemic microorganisms

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International Journal of Sediment Research 2023年第3期38卷 446-454页

作者： Seyed Hamidreza Sadeghi Masumeh Ashgevar Heydari atefeh Jafarpoor Department of Watershed Management Engineering Faculty of Natural Resources Tarbiat Modares University Agrohydrology Research Group Tarbiat Modares University

Consecutive rainfalls, due to changes in antecedent moisture, alter soil erosion processes, necessitating the implementation of suitable soil loss control methods. Biological soil microorganism approaches have been applied to control soil loss. However, information on the involvement of microorganisms in the soil loss and rill erosion processes has yet to be supplied. In this study, the individual and combined inoculation of cyanobacteria and bacteria was investigated during five consecutive rainfalls with an interval of three days, an intensity of 50 mm/h, and a duration of 30 min. Also, additional runoff simulation of about 2.18 ± 0.32 L/min was done in mid-sized experimental plots(i.e., 6 m × 1 m × 0.5 m) induced by rill erosion. These experiments were done at the Rainfall and Soil Erosion Simulation Laboratory of the Faculty of Natural Resources, Tarbiat Modares University, Iran. Soil loss and sediment concentration were measured during five consecutive rainfalls in the study treatments and compared with the control treatment. Results showed that the highest soil loss of 30,810.70 g occurred at the control treatment in the first rainfall. In contrast, individual and combined inoculation treatments of cyanobacteria and bacteria with the secretion of polysaccharides and resulting stability of aggregates decreased soil loss significantly(p < 0.01) by 99.65%, 99.91%, and 100.00%, respectively, compared to the control *** the combined treatment of cyanobacteria and bacteria, soil loss further decreased by 68.38%, 70.33%,and 7.27% for 2 through 4, respectively, events and increased by 19.96% in the fifth event, and sediment concentration also decreased by 68.88% and 59.86% for the second and third events, respectively and increased by 4.66% and 83.30% for the fourth and fifth events, respectively. The best performance was found in the combined inoculation in consecutive rainfalls(i.e., from the second to the fifth event), and the bacteria treatment and the co

关键词： Biocrust Linear erosion Sediment accessibility Soil and water bio-management

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