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Differential RNA Editing of Mitochondrial Genes in WA-Cytoplasmic Based Male Sterile Line Pusa 6A, and Its Maintainer and Restorer Lines

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Rice science 2019年第5期26卷 282-289页

作者： Umakanta NGANGKHAM Swarup kumar PARIDA ashok kumar singh Trilochan MOHAPATRA National Research Centre on Plant Biotechnology New Delhi 110012India Indian Council of Agricultural Research-Research Complex for North East Hill Region Indian Council of Agricultural ResearchUmiam 793103India National Institute of Plant Genome Research New Delhi 110067India Division of Genetics Indian Agricultural Research InstituteNew Delhi 110012India Indian Council of Agricultural Research New Delhi 110001India

RNA editing changes the nucleotides at the transcript level of mitochondrial genes which results in synthesis of functional *** study was designed to find the editing sites which could be implicated in male fertility restoration and to develop editing based markers for differentiation of cytoplasmic male sterility and maintainer lines from each *** and RNA from young panicles were isolated from three-line system of hybrid rice PRH10,wild abortive(WA)cytoplasm based male sterile(A line Pusa 6A),maintainer(B line Pusa 6B)and restorer(R line PRR78)*** 6A and PRR78 having the same WA cytoplasm are allo-nuclear and iso-cytpolasmic *** genomic and cDNA amplicons for eight mitochondrial genes(18SrRNA,atp6,atp9,cobII,coxI,coxIII,nadI and rps3)were sequenced and *** in genomic and cDNA sequences were considered as *** hundred and thirty editing sites having base substitution or insertion/deletion were identified with the highest in 18SrRNA(5.74%)and the lowest in coxI(0.60%).The highest editing sites were observed in fertile maintainer Pusa 6B followed by PRR78 and Pusa 6A,of which random five editing sites in five different rice mitochondrial transcripts namely atp9,cobII,coxIII,rps3 and 18SrRNA were chosen and validated through cleaved amplified polymorphism sequence(CAPS)analysis and found to be partially edited in four *** identical editing sites of different mitochondrial genes from maintainer and restorer lines might reflect their possible contribution to fertility restoration of sterile WA cytoplasm.

关键词： cleaved amplified polymorphism sequence cytoplasmic male sterility hybrid rice RNAediting mitochondrial gene wild abortive nucleotide

Preliminary slope stability analysis and discontinuities driven susceptibility zonation along a crucial highway corridor in higher Himalaya, India

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Journal of Mountain Science 2020年第4期17卷 801-823页

作者： Barsa ACHARYA Kripamoy SARKAR ashok kumar singh Sowmiya CHAWLA Department of Applied Geology Indian Institute of Technology(Indian School of Mines)Dhanbad826004India Department of Civil Engineering Indian Institute of Technology(Indian School of Mines)Dhanbad826004India

Identification of failure susceptible slopes through different rock engineering approach is highly valuable in landslide risk management along crucial highway corridors in the high mountainous region. In this study, a critical highway(NH-5) segment in higher Himalaya has been investigated using the various rock mass characterization schemes based on detailed field observations. Since the highway corridor is highly susceptible to discontinuities-driven failures, consisting of jointed rock masses;Mean and Combined kinematic feasibility analysis has been performed for 20 highway slopes. Observed slope mass classes have been compared to the feasibility percentage of discontinuities driven failures(wedge, toppling, and planar) and accordingly the kinematic feasibility zonation along highway segment has been done for each as well as overall failure types. Based on the slope mass conditions and discontinuities driven failures probability(%), responsive remedial measures have been proposed for individual highway slopes to ensure safe and uninterrupted transportation.

关键词： Slope characterization Kinematic analysis Failure zonation map Himalayan highways Landslide Slope stability

Influence of temperature dependent heat source/sink on transient MHD free convective flow in an infinite rigid impermeable vertical cylinder with chemical reaction

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Propulsion and Power Research 2023年第4期12卷 568-583页

作者： Naveen Dwivedi ashok kumar singh Nirmal C.Sacheti Department of Mathematics Institute of ScienceBanaras Hindu UniversityVaranasi 221005India Department of Mathematics Sahu Jain CollegeNajibabadAffiliated to M.J.P.Rohilkhand UniversityBareilly 246763India Retired Professor Department of MathematicsCollege of ScienceSultan Qaboos UniversityAl Khod PC 123MuscatSultanate of Oman

It is increasingly apparent that the inclusion of mass transfer aspects,together with certain thermal conditions,in the momentum and energy equations governing MHD flows leads to a numbers of real life *** this in view,we have attempted an exact analysis of heat and mass transfer aspects in transient hydromagnetic free convective flow of an incompressible viscous fluid through a vertical pipe under an externally applied magnetic field,assuming presence of chemical reaction and heat source/*** governing PDEs,which simplify to a set of 3 linear ODEs in the physical set up considered here,have been solved using Laplace transform technique,with solutions for key physical variables presented in the term of Bessel and modified Bessel *** influence of governing non-dimensional parameters,namely,Hartmann number,Schmidt number,source/sink parameter,Prandtl number and chemical reaction parameter,has been illustrated on the developing velocity and some concentration *** important quantities of engineering interest-surface skin friction and volumetric flow rates-have been computed too and *** notable finding worth mentioning are:(a)heat source presence causes higher fluid velocity as compared to the heat sink;(b)all important surface shear stress can be suitably controlled,among others,by chemical reaction parameter and Schmidt *** key challenge of this study has been to obtain exact closed-form solutions of the field equations,including cumbersome Laplace *** study finds innovative applications in the emerging fields such as magnetic materials processing,chemical processes,solar energy systems,etc.

关键词： Circular cylinder Hydromagnetic transient flow Chemical reaction Source/sink Free convection

Effect of Heat Source/Sink on Free Convective Flow of a Polar Fluid between Vertical Concentric Annuli

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Journal of Applied Mathematics and Physics 2017年第9期5卷 1750-1762页

作者： Arun kumar singh ashok kumar singh Department of Mathematics Institute of Science Banaras Hindu University Varanasi India

The purpose of this paper is to find the effect of heat source/sink parameter on free convective flow of a polar fluid in open-ended vertical concentric annuli. Exact solutions of the non-dimensional differential equations describing the flow model have been obtained one by one for two different cases of source and sink. To observe the effect of the physical parameters such as source/sink and vertex viscosity, the numerical results of the velocity and microrotational velocity are finally shown on the graphs.

关键词： Polar Fluid Free Convection Isothermal and Constant Heat Flux Cases Temperature Dependent Heat Source/Sink Vertical Annuli

Post-adoption behaviour of farmers towards soil and water conservation technologies of watershed management in India

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International Soil and Water Conservation Research 2015年第3期3卷 161-169页

作者： Gopal Lal Bagdi Prasanta kumar Mishra Ravi Sankar Kurothe Swarn Lata Arya Shekhargouda Linganagouda Patil ashok kumar singh Bankey Bihari Om Prakash ashok kumar Palanisamy Sundarambal ICAR–Central Sheep and Wool Research Institute Avikanagar 304501RajasthanIndia ICAR–Indian Institute of Soil and Water Conservation Dehradun 248195UttrakhandIndia

The Indian Institute of Soil and Water Conservation(IISWC)and its Research Centres have developed many successful model watershed projects in India in the past and implemented many Soil and Water Conservation(SWC)technologies for sustainable watershed *** many evaluation studies were conducted on these projects in the past,there has been no assessment of the post-adoption status of the SWC technologies over a longer *** was imperative to appraise the behaviour of the farmers with regard to the continuance or discontinuance of the technologies adopted,diffusion or infusion that took place and technological gaps that occurred in due course of time in the post watershed ***,it was realized that the postadoption behaviour of beneficiary farmers who have adopted different soil and water conservation technologies for watershed management projects should be studied in *** research study was initiated in 2012 as a core project at Vasad as the lead Centre along with IISWC headquarter Dehradun,and Centres Agra,Bellary,Chandigarh,Datia,Kota&Ooty,with the specific objectives of the study to measure the extent of post-adoption behaviour(continued-adoption,discontinuance,technological gap,diffusion and infusion)of farmers towards the adopted SWC technologies of watershed *** the present study various indices regarding continued adoption,dis-adoption(discontinuance),technological gap,diffusion,infusion regarding soil and water conservation technologies for watershed management were developed for measurement of post-adoption behaviour of *** was revealed that a little less than three-fourth(73%)of SWC technologies continued to be adopted and more than onefourth(27%)were discontinued by *** of the total continue adopted SWC technologies by farmers,a little less than onefifth(19%)of technologies continued to be adopted with a technological *** than one-fourth(28%)of SWC technologies were also diffused to other fa

关键词： Post-adoption Soil and water conservation Watershed Management

Analysis of Entropy Generation in a Rectangular Porous Duct

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Journal of Applied Mathematics and Physics 2016年第7期4卷 1336-1343页

作者： Shyam Lal Yadav ashok kumar singh Department of Mathematics Institute of Science Banaras Hindu University Varanasi India

In this paper, we have considered a fully developed flow of a viscous incompressible fluid in a rectangular porous duct saturated with the same fluid. The duct is heated from the bottom for forced and mixed convection. The Brinkman model is used to simulate the momentum transfer in the porous duct. Using the momentum and thermal energy equations, the entropy generation has been obtained due to the heat transfer, viscous and Darcy dissipations. It is found from the mathematical analysis that the entropy generation is double when the viscous as well as the Darcy dissipations terms are taken in the thermal energy equation in comparison when the viscous as well as the Darcy dissipations terms are not taken in the thermal energy equation. This result clearly shows that there is no need of taking the viscous and Darcy dissipations terms in the thermal energy equation to obtain the entropy generation.

关键词： Brinkman Model Heat Flux Fully Developed Flow Viscosity Ratio Entropy Generation

Intelligent Diagnostic Method for Ageing Analysis of Transformer

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Energy and Power Engineering 2012年第2期4卷 53-58页

作者： Anil kumar Kori Arvind kumar Sharma ashok kumar singh Bhadoriya Department of Electrical Engineering Jabalpur Engineering College Jabalpur Indi Department of Electrical Engineering Jabalpur Engineering College Jabalpur India Rajiv Gandhi Technical University Bhopal India

The fuzzy method is proposed for ageing analysis of transformer. The fuzzy controller is used for various input and one output, strictly depends on the number of membership function and there rule base and the type of the defuzzification method. The ageing of transformers is influenced by short term and long term over loads, number and intensity of short circuits, incidence of lightning, and internal faults. The recent development of various techniques for detecting the incipient fault conditions have been improved to some extent;the life expectancy of transformers by resorting to corrective actions in time. The ageing behavior is likely to be different for different types of transformers. The life span of the transformer, thus depends initially on the design and quality of manufacture and later on service conditions and maintenance standard, these factors vary considerably and affect the useful span of service life which therefore needs to be taken into account for residual life assessment. During the natural ageing of transformers, the insulation of winding deteriorates. Cellulose insulation degrades due to heating or electrical breakdown which is dissolved in oil. Hence, the chemical analysis of the Transformer oil gives evidence of changes that are taking place in the winding insulation during operation. Deterioration in transformer cellulose decreases both its electrical and mechanical strength. In this paper a novel fuzzy based algorithm has been implemented on three samples of power transformer oil.

关键词： Transformer Oil Paper Insulation Electrical Breakdown Ageing Analysis Fuzzy Logic Controller

Microstructure, Texture, Mechanical and Ballistic Properties Correlation of a Hot Rolled and Peak Aged AA-7017 Alloy Plate at Surface and Centre

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Materials Sciences and Applications 2017年第13期8卷 992-1013页

作者： Pradipta kumar Jena Kandikattu Siva kumar Rajiv kumar Mandal ashok kumar singh Defence Metallurgical Research Laboratory Hyderabad India Department of Metallurgical Engineering IIT (BHU) Varanasi India

The present work describes microstructure, texture, mechanical and ballistic properties correlation of a hot rolled and peak aged AA-7017 alloy plate at surface and centre. Both the microstructures and textures are different on the surface and centre of the plate. The surface of the plate shows recrystallized grains and a weak over all texture. The centre of the plate displays elongated grains and a sharp texture. Tensile properties, hardness and impact toughness are evaluated at surface and centre of the plate. It is observed that strength and hardness is high at centre, whereas ductility and impact toughness is more at the surface. Ballistic properties of the plate at centre and surface are measured by impacting against two different 7.62 mm deformable projectiles. The plates impacted on the surface shows better ballistic resistance. Ballistic performance of the plate at surface and centre has been correlated with the microstructure, texture and mechanical properties.

关键词： Ballistic Performance AA 7017 Alloy Microstructure Texture Mechanical Property Ballistic Property Anisotropy

两种草蛉对取食棉花的扶桑绵粉蚧的嗅觉反应(英文)

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昆虫学报 2010年第5期53卷 497-507页

作者： Sudhida GAUTAM ashok kumar singh Ram Dass GAUTAM Insect Bchaviour Laboratory Department of Zoology University of Delhi New Delhi110005 India Biological Control Laboratory Division of Entomology Indian Agricultural Research Institute New Delhi-110012 India

捕食性的普通草蛉Chrysoperla sp.(carnea group)和安平草蛉Mallada desjardinsi(Navas)(脉翅目:草蛉科)的寄主-生境定位取决于其觅食行为以及对各阶段释放的植物气味的行为反应。本研究调查了这两种交配的草蛉成虫以及取食棉花的扶桑... 详细信息

捕食性的普通草蛉Chrysoperla sp.(carnea group)和安平草蛉Mallada desjardinsi(Navas)(脉翅目:草蛉科)的寄主-生境定位取决于其觅食行为以及对各阶段释放的植物气味的行为反应。本研究调查了这两种交配的草蛉成虫以及取食棉花的扶桑绵粉蚧Phenacoccus solenopsis的反应,以便了解它们之间的三重营养关系。结果表明:草蛉雄虫和雌虫均能感受到棉花植株释放的绿叶挥发物;植株的各个阶段均可使捕食性昆虫和绵粉蚧定位。在受绵粉蚧为害的棉叶上饱和烃含量高,说明草蛉成虫的反应更为强烈;在植株受为害期和开花期释放捕食性昆虫,可提高田间捕食性昆虫的作用。这些结果提示有效的生物防治取决于捕食性昆虫在植株上的天然定殖能力以及对害虫的吞食能力。

关键词：普通草蛉安平草蛉扶桑绵粉蚧三重营养关系棉花绿叶挥发物