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Suppression of the External MitochondrialNADPH Dehydrogenase, NDB1, in arabidopsisthaliana Affects Central Metabolism andVegetative Growth

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Molecular Plant 2014年第2期7卷 356-368页

作者： Wallström S Florez-Sarasa I araújo wl Aidemark M Fernández-Fernández M Fernie AR Ribas-Carbó M Rasmusson AG Department of Biology Lund University Biology building Sölvegatan 35 SE-223 62 Lund Sweden

Ca2＋-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPHdehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript wassuppressed by 80% in arabidopsis thaliana plants, and external Ca2＋-dependent NADPH dehydrogenase activity becameundetectable in isolated mitochondria. This was linked to a decreased level of NADP＋ in rosettes of the transgenic ***-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of 14C-glucose was increased. On soil, NDBl-suppressing plants had a decreased vegetative biomass, but leaf maximumquantum efficiency of photosystem Ⅱ and CO2 assimilation rates, as well as total respiration, were similar to the wild-type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealeddecreased levels of sugars, citric acid cycle intermediates, and amino acids in the transgenic lines. The NDBl-suppressioninduced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. Thetranscriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thalianaoverexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP（H）reduction levels, which in turn affect central metabolism and growth, and interact with defense signaling.

关键词： citric acid cycle metabolic profiling mitochondrial respiration NADPH RNA interference type II NAD(P)Hdehydrogenase vegetative growth

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Aseismic Reliability Analysis Approach for Offshore Jacket Platform Structures

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China Ocean Engineering 1998年第4期13卷 375-382页

作者： Zhuang, YZ Jin, wl Zhejiang Univ Dept Civil Engn Hangzhou 310027 Peoples R China

By means of nonlinear pushover collapse analysis approach, the aseismic reliability analyses of two offshore jacket platforms in the Bohai Gulf in China are studied according to their ocean location and environmental loadings there. On the basis of those analyses, an aseismic reliability analysis approach is presented. The results show that the aseismic reliability of those platforms is high. Also it is proved that this aseismic reliability analysis approach is simple, practical and reliable.

关键词： offshore jacket platform aseismic reliability nonlinear pushover collapse analysis

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SYNTHESIS AND PROPERTIES OF SOME NEW POLYIMIDES CONTAINING BITHIAZOLE RINGS

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Chinese Chemical Letters 1996年第4期7卷 313-316页

作者： Sun, wl Lu, FC ACAD SINICA INST CHEMBEIJING 100080PEOPLES R CHINA

Some new polyimides containing bithiazole rings were prepared by reacting 2,2'-diamino-4,4'-bisthiazole with different aromatic dianhydrides. The polyimides obtained had inherent viscosities of 0.37-0.82 dL/g. Thermogravimetric analysis of the polyimides showed good thermal stability, the temperature at 5% weight loss being from 450 degrees C to 560 degrees C. The permeability of two polymer membranes to H-2, O-2 and N-2 was determined.

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A Registry-Based Observational Study on the Maternal and Fetal Outcomes of COVID-19 Patients in Hong Kong,China

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Maternal-Fetal Medicine 2024年第3期6卷 156-163页

作者： Hillary HY Leung Teresa wl Ma Florrie NY Yu Meliza CW Kong Tsz Kin Lo Pauline PL So Wing-Cheong Leung Wendy Shu Ka Wang Cheung Sakita Moungmaithong Chi Chiu Wang Liona C.Poon Department of Obstetrics and Gynaecology The Chinese University of HongKongHong Kong 999077China Department of Obstetrics and Gynaecology Queen Elizabeth HospitalHong Kong 999077China Department of Obstetrics and Gynaecology United Christian HospitalHong Kong 999077China Department of Obstetrics and Gynaecology Princess Margaret HospitalHong Kong 999077China Department of Obstetrics and Gynaecology Tuen Mun HospitalHong Kong 999077China Department of Obstetrics and Gynaecology Kwong Wah HospitalHong Kong 999077China Department of Obstetrics and Gynaecology Pamela Youde Nethersole Eastern HospitalHong Kong 999077China Department of Obstetrics and Gynaecology Queen Mary HospitalHong Kong 999077China

Objective:To report the clinical maternal and fetal outcomes of pregnant women with coronavirus disease 2019(COVID-19),along with any associated pregnancy complications,in Hong Kong,China,and to assess the impact of COVID-19 vaccination on these ***:This prospective registry-based observational study included pregnant women who were recruited through convenient sampling and had a laboratory-confirmed severe acute respiratory syndrome coronavirus 2 infection with a cycle threshold(Ct)value result available on admission to eight local hospitals in Hong Kong,*** on clinical symptoms,laboratory results,medical treatments,delivery timing and mode,and pregnancy complications were extracted from the Hospital Authority’s electronic medical record ***,fetal,and pregnancy outcomes were compared between unvaccinated pregnant women with COVID-19 and those who had received at least one dose of COVID-19 vaccine before *** continuous variables and categorical variables were analyzed using the Mann-Whitney U test and the Pearson’s chi-squared test respectively.A P value less than 0.05 was considered statistically ***:A total of 164 pregnant women were included,of whom 78(47.56%)were ***-19 was diagnosed before 28 weeks’gestation in 30(18.29%),while 134(81.71%)were diagnosed at or after 28 weeks’***-two(37.80%)women received at least one dose of COVID-19 *** were no significant differences between vaccinated and unvaccinated groups in the time interval between COVID-19 diagnosis and delivery,the Ct value,and the gestational age at infection onset or delivery(P>0.05).The majority of women were symptomatic at diagnosis regardless of vaccination status 55(88.71%)in vaccinated group vs.78(76.47%)in unvaccinated group(P=0.052).Symptoms did not significantly differ between groups except for cough(62.90%vs.47.06%,P=0.049).The overall rate of severe COVID-19 in pregnant women was *** t

关键词： COVID-19 Maternal outcomes Fetal outcomes Pregnancy complications Severe COVID-19

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Nucleation and Oriented Textured Growth of Diamond Films on Si(100) via Electron Emission in Hot Filament Chemical Vapor Deposition

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journal of Materials Science & Technology 2000年第1期16卷 19-22页

作者： Wang, wl Liao, KJ Wang, BB Chongqing Univ Dept Appl Phys Chongqing 400044 Peoples R China

Oriented textured diamond films were obtained on Si(100) substrate via electron emission in hot filament chemical vapor deposition (HFCVD). A de bias voltage relative to the filament was applied to the tungsten electrode between the substrate and the filament. The nucleation and subsequent growth of diamond films were characterized by scanning electron microscopy and Raman spectroscopy. The experimental results showed that the electron emission from the diamond coating on the electrode played a critical role during the nucleation. The maximum value of nucleation density was up to 10(11) cm(-2) on pristine Si surface at emission current of 250 mA. The effect of the electron emission on the reactive gas composition was analyzed by in situ infrared absorption, indicating that the concentration of CH3 and C2H2 near the substrate surface was extremely increased. This may be responsible for the enhanced nucleation by electron emission.

关键词： Diamond films

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A New Triterpenoid Glycoside from Dichotomanthes tristaniaecarpa

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Chinese Chemical Letters 2000年第11期11卷 1013-1014页

作者： Mei, wl Luo, XD Chen, CX Acad Sinica Kunming Inst Bot Lab Phytochem Kunming 650204 Peoples R China

A new triterpenoid glycoside 1, 1 beta, 2 alpha, 3 alpha, 19 alpha -tetrahydroxyurs-12-en-28-oic acid beta -D glucopyranosyl ester was isolated from Dichotomanthes tristaniaecarpa. The structure was elucidated on the ... 详细信息

关键词： triterpenoid glucoside Dichotomanthes tristaniaecarp Rosaceae

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Solid and liquid phase equilibria in mixtures of N, N-dimethylacetamide with chlorinated hydrocarbons

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Chinese Science Bulletin 1997年第11期42卷 918-921页

作者： Che, GQ Peng, wl Che, B Department of Chemistry Zhongshan University Guangzhou China

IN refs.[1,2],we studied the solid and liquid phase equilibria in mixtures of tetra-chloromethane with oxygen and nitrogen-containing compounds,intermolecular interactionswith the formation of solid molecular addition... 详细信息

关键词： phase diagram solid and liquid equilibrium N N-dimethylacetamide.

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Ab initio molecular orbital study of structure on aluminium borides AlB_n (n = 1- 5) series

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Chinese Science Bulletin 1997年第11期42卷 907-910页

作者： Wu, HS Xu, XH Zhou, wl Department of Chemistry Shanxi Normal University Linfen 041004 China

RECENTLY,great attention has been paid to the physical and chemical properties of atomic andsemiconductor *** the properties of AlB_n atomic cluster,experimental studieshave been reported,yet theoretical studies have not been *** borides(AlB_n)have been widely used to change the fiber strength in mechanical engineering and to adjust

关键词： aluminium boride ab initio molecular orbital binding energy.

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Synthesis of Chitosan Quaternary Ammonium Salts

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Chinese Chemical Letters 2001年第12期12卷 1081-1084页

作者： Xu, wl Wu, J Fu, CL Zhejiang Univ Dept Chem Hua Jia Chi Sect Hangzhou 310029 Peoples R China

A series of N-alkyl or N-aryl chitosan quaternary ammonium salts were prepared via Schiffs base intermediates. Quaternization of N-substituted chitosan derivatives was carried out using methyl iodide to produce water-soluble cationic chitosan quaternary ammonium salt. The products were characterized by IR, (HNMR)-H-1 and elemental analysis. The degree of substitution of chitosan quaternary ammonium salt was calculated by elemental analysis.

关键词： synthesis quaternization chitosan quaternary ammonium salts

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Expression of gap junction genes connexin 32,connexin 43 and their proteins in hepatocellular carcinoma and normal liver tissues

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World journal of Gastroenterology 2000年第1期6卷 66-69页

作者： Ma XD Sui YF Wang wl Department of Pathology the Fourth Military Medical University17 Changle XiluXi'an 710033Shaanxi ProvinceChina

AIM To investigate the significance andmechanism of cx32 mRNA,cx43 mRNA and theirproteins in *** Sixty-one cases of HCC and 14cases of normal liver tissues were detected byimmunohistochemical and in situ hybridization(ISH)*** In HCC grades Ⅰ,Ⅱ,Ⅲ and normalliver tissues,the positive rates of Cx32 proteinwere 55.6%,42.1%,18.2% and 92.9%,*** detection rates of Cx43 proteinwere 44.4%,26.3%,12.1% and 78.6%,*** was significant difference inCx32 and Cx43 protein between HCC and normalliver tissues(P0.05).CONCLUSION The aberrant location of Cx32and Cx43 proteins could be responsible forprogression of hepatocarcinogenesis,and thedefect of cx genes in post-translationalprocessing might be the possible mechanism.

关键词： Subject headings connexin gap junction liver neoplasm immunohistochemistry in situ hybridization carcinoma hepatocellular gene expression

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