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Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

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World Journal of Gastroenterology 2023年第22期29卷 3440-3468页

作者： Henrique Inhauser Riceti magalhães Felipe Alexandre machado Roberta Figueiroa Souza marcos antônio Ferreira caetano Vanessa Ribeiro Figliuolo Robson Coutinho-Silva Patricia Castelucci Department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of São PauloSão Paulo 05508-270Brazil Department of Anatomy University of São PauloSão Paulo 05508-000Brazil Biophysics Institute Carlos Chagas Filho Federal University of Rio de JaneiroRio de Janeiro 21941-902Brazil

BACKGROUND The literature indicates that the enteric nervous system is affected in inflammatory bowel diseases(IBDs)and that the P2X7 receptor triggers neuronal ***,the mechanism by which enteric neurons are lost in IBDs is *** To study the role of the caspase-3 and nuclear factor kappa B(NF-κB)pathways in myenteric neurons in a P2X7 receptor knockout(KO)mouse model of *** Forty male wild-type(WT)C57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid(colitis group).mice in the sham groups were injected with *** mice were divided into eight groups(n=5):The WT sham 24 h and 4 d groups,the WT colitis 24 h and 4 d groups,the KO sham 24 h and 4 d groups,and the KO colitis 24 h and 4 d *** disease activity index(DAI)was analyzed,the distal colon was collected for immunohistochemistry analyses,and immunofluorescence was performed to identify neurons immunoreactive(ir)for calretinin,P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,and total NF-κ*** analyzed the number of calretinin-ir and P2X7 receptor-ir neurons per ganglion,the neuronal profile area(μm^(2)),and corrected total cell fluorescence(CTCF).RESULTS Cells double labeled for calretinin and P2X7 receptor,cleaved caspase-3,total caspase-3,phospho-NF-κB,or total NF-κB were observed in the WT colitis 24 h and 4 d *** number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups,respectively(2.10±0.13 vs 3.33±0.17,Pantly different between the KO *** calretinin-ir neuronal profile area was increased in the WT colitis 24 h group compared to the WT sham 24 h group(312.60±7.85 vs 278.41±6.65,Pmpared to the WT sham 4 d group(104.63±2.49 vs 117.41±1.14,Pmber of

关键词： Cell death Enteric nervous system Gastroenterology Inflammatory bowel diseases P2X7 receptor Purinergic signaling

Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and gastric cancer

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World Journal of Gastroenterology 2005年第42期11卷 6593-6600页

作者： márcia Cristina Duarte Jucimara Colombo Andrea Regina Baptista Rossit Alaor caetano Aldenis Albaneze Borim Durval Wornrath Ana Elizabete Silva UNESP-S(a|~)o Paulo State University Department of Biology Campus S(a|~)o José do Rio Preto SP Brazil FAmERP- S(a|~)o José do Rio Preto School of medicine microorganism Investigation Center S(a|~)o José do Rio Preto SP Brazil FAmERP-S(a|~)o José do Rio Preto School of medicine Hospital de Base S(a|~)o José do Rio Preto SP Brazil Pio Ⅻ Foundation Barretos SP Brazil UNESP- S(a|~)o Paulo State University Department of Biology Campus S(a|~)o José do Rio Preto SP Brazil

AIm： TO evaluate the association between poly- morphisms XRCC1 Arg194Trp and Arg399GIn and XRCC3 Thr241met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population. mETHODS： Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis （CG） and 160 patients with gastric cancer （GC）, matched to 202 （Cl） and 150 （C2） controls, respectively. RESULTS： No differences were observed among the studied groups with regard to the genotype distribution of XRCC1 codons 194 and 399 and of XRCC3 codon 241. However, the combined analyses of the three variant alleles （194Trp, 399Gin and 241met） showed an increased risk for chronic gastritis when compared to the GC group. moreover, an interaction between the polymorphic alleles and demographic and environmental factors was observed in the CG and GC groups. XRCC1 194Trp was associated with smoking in the CG group, while the variant alleles XRCC1 399Gin and XRCC3 241met were related with gender, smoking, drinking and Hpylori infection in the CG and GC groups. CONCLUSION： Our results showed no evidence of a rela-tionship between the polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3 Thr241met and the risk of chronic gastritis and gastric cancer in the Brazilianpopulation, but the combined effect of these variants may interact to increase the risk for chronic gastritis, considered a premalignant lesion. Our data also indicate a gene-environment interaction in the susceptibility to chronic gastritis and gastric cancer.

关键词： Gastric cancer Gastritis XRCC1 XRCC3 Polymorphism Environmental exposure

Rare earth metal as a dopant element:Cerium ion as an articulator in hexavalent chromium removal by magnetic iron oxides

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Journal of Rare Earths 2023年第10期41卷 1616-1626,I0006页

作者： Pricila maria Batista Chagas maísa m.m.Lima Aline Aparecida caetano Lucas Bragança Carvalho Nayra maria de Abreu Piva maria Eduarda Resende Luiz Iara do Rosário Guimarães Laboratory of Environmental Catalysis and New materials Department of ChemistryFederal University of LavrasCEP 37200-900LavrasMGBrazil Department of Viticulture&Enology University of CaliforniaDavisCA95616USA Institute of Science and Technology São Paulo State University18087-180SorocabaBrazil

This study highlights introducing the rare earth metal cerium(Ce(Ⅳ))into the structure of magnetite(Fe_(3)O_(4))to achieve enhanced adsorptive properties for the removal of chromium(Cr(VI))from an aqueous *** ratios of Ce(Ⅳ)were introduced into the iron oxide matrix,termed FeCe-5,FeCe-10,and *** numerical values correspond to the nominal content of the dopant element added to the synthesis *** solid materials were characterized for morphology and chemical structure,and N_(2)physical adsorption/desorption measurements were *** solid materials doped with Ce(IV)have a high surface area compared to Fe_(3)O_(4),and the solid material with the highest content of the dopant ion(Ce(IV))has a 4-fold greater surface *** increase in the dopant content in the iron oxide structure leads to a total chromium removal of 93.3%.Isotherms studies on the solid materials show that chromium adsorption follows the Langmuir *** adsorption capacity to Fe_(3)O_(4)is 12.59 mg/g and FeCe-10 is 22.49 mg/g at 35℃.By fitting the kinetic and isothermal models,it is found that for the Fe_(3)O_(4)and FeCe-10 materials Cr(VI)removal occurs in very different ways,attributed to the different surface areas and compositions of the oxide,with the formation of the goethite(α-FeOOH)*** FeCe-10reuse process was performed and the removal capacity the Cr(VI)is reduced after the first *** result is attributed to a strong and irreversible adsorption of Cr(VI)on the FeCe-10.

关键词： Bimetallic oxides Fe-Ce interaction Oxygen vacancies Adsorption Cr(Ⅵ) Rare earths

A large epeiric methanogenic Bambuí sea in the core of Gondwana supercontinent?

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Geoscience Frontiers 2021年第1期12卷 203-218页

作者： Sergio caetano-Filho Pierre Sansjofre magali Ader Gustavo m.Paula-Santos Cristian Guacaneme marly Babinski Carolina Bedoya-Rueda matheus Kuchenbecker Humberto L.S.Reis Ricardo I.F.Trindade Universidade de Sao Paulo Instituto de GeocienciasRua do Lago562-Sao Paulo05508-080Brazil mNHN Sorbonne UniversiteCNRS UMR 7590Institut de Mineralogiede Physique des Materiaux et de Cosmochimie75005ParisFrance Universite de Paris Institut de Physique du Globe de ParisCNRSF-75005ParisFrance Universidade Estadual de Campinas Instituto de GeocienciasRua Carlos Gomes250-Campinas13083-855Brazil Universidade Federal dos Vales do Jequitinhonha e mucuri Instituto de Ciencia e TecnologiaLaboratorio de Estudos TectonicosRodovia MGT 367Km 583-Diamantina39100-000Brazil Universidade Federal de Ouro Preto Departamento de Geologia-Escola de MinasLaboratiorio de Modelagem TectonicaCampus Morro do Cruzeiro-Ouro Preto35400-000Brazil Universidade de Sao Paulo Instituto de AstronomiaGeofísica e Ciencias AtmosfericasRua do Matao1226-Sao Paulo05508-090Brazil

Carbon isotope compositions of both sedimentary carbonate and organic matter can be used as key proxies of the global carbon cycle and of its evolution through time,as long as they are acquired from waters where the dissolved inorganic carbon(DIC)is in isotope equilibrium with the atmospheric ***,in shallow water platforms and epeiric settings,the influence of local to regional parameters on carbon cycling may lead to DIG isotope variations unrelated to the global carbon *** may be especially true for the terminal Neoproterozoic,when Gondwana assembly isolated waters masses from the global ocean,and extreme positive and negative carbon isotope excursions are recorded,potentially decoupled from global *** improve our understanding on the type of information recorded by these excursions,we investigate the pairedδ^13Ccarb andδ^13Corg evolution for an increasingly restricted late Ediacaran-Cambrian foreland system in the West Gondwana interior:the basal Bambui *** succession represents a 1~(st)-order sedimentary sequence and records two majorδ^13Ccarb excursions in its two lowermost lower-rank *** basal cap carbonate interval at the base of the first sequence,deposited when the basin was connected to the ocean,hosts antithetical negative and positive excursions forδ^13Ccarb andδ^13Corg,respectively,resulting inΔ^13C values lower than 25‰.From the top of the basal sequence upwards,an extremely positiveδ^13Ccarb excursion is coupled toδ^13Corg,reaching values of+14‰and-14‰,*** positive excursion represents a remarkable basin-wide carbon isotope feature of the Bambui Group that occurs with only minor changes inΔ^13C values,suggesting change in the DIC isotope *** argue that this regional isotopic excursion is related to a disconnection between the intrabasinal and the global carbon *** extreme carbon isotope excursion may have been a product of a disequilibria between the basin DIC and atmospheric CO2

关键词： methanogenesis Ediacaran Cambrian Carbon isotopes Sao Francisco Basin Bambui Group

Upregulation of the APE1 and H2AX genes and miRNAs involved in DNA damage response and repair in gastric cancer

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Genes & Diseases 2019年第2期6卷 176-184页

作者： Fernanda S.manoel-caetano Ana Flávia T.Rossi Gabriela Calvet de morais Fábio Eduardo Severino Ana Elizabete Silva Department of Biology UNESPSāo Paulo State UniversityCampus of Sāo Josédo Rio PretoRua Cristòvāo Colombo226515.054-000Sāo Josédo Rio PretoSāo PauloBrazil Department of Surgery and Orthopedics Faculty of MedicineUNESPSāo Paulo State UniversityCampus of BotucatuAv.Prof.Mário Rubens Guimarāes Montenegros/n18.618-687BotucatuSāo PauloBrazil

Gastric cancer remains one of the leading causes of cancer-related death worldwide,and most of the cases are associated with Helicobacter pylori *** bacterium promotes the production of reactive oxygen species(ROS),which cause DNA damage in gastric epithelial *** this study,we evaluated the expression of important genes involved in the recognition of DNA damage(ATm,ATR,and H2AX)and ROS-induced damage repair(APE1)and the expression of some miRNAs(miR-15a,miR-21,miR-24,miR-421 and miR-605)that target genes involved in the DNA damage response(DDR)in 31 fresh tissues of gastric *** v3.1.1 was used to construct the postulated miRNA:mRNA interaction *** performed by real-time quantitative PCR exhibited significantly increased levels of the APE1(RQ=2.55,pmiR-421 and miR-605 in the gastric cancer *** addition,significantly elevated levels of miR-21,miR-24 and miR-421 were observed in diffuse-type gastric *** analysis reinforced some of the gene:gene(ATm/ATR/H2AX)and miRNA:mRNA relationships obtained also with the interaction ***,our findings show that tumor cells from gastric cancer presents deregulation of genes and miRNAs that participate in the recognition and repair of DNA damage,which could confer an advantage to cell survival and proliferation in the tumor microenvironment.

关键词： DNA damage response DNA repair Gastric cancer Gene expression microRNA

Experimental Study of Soot Volume Fraction Applied in Laminar Diffusion Flames

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modern mechanical Engineering 2013年第4期3卷 137-141页

作者： N. R. caetano F. m. Pereira H. A. Vielmo F. T. van der Lann Federal University of Rio Grande do Sul DEMEC-UFRGS Porto Alegre Brazil

The soot emission has been the focus of many studies due to applications in industry and the prejudicial effects caused to the environment. The presence of soot is important to the heat transfer in boilers and combustion chambers, contributing significantly to efficiency increases. In controversy, the inhaled soot may cause respiratory system damage and even cancer. Another important point is the contribution to the greenhouse effect. Therefore, the aim of this work is to analyse the soot emission in laminar diffusion flames produced from commercial fuels stabilized on a burner representative of industrial applications. methane, vehicular natural gas and liquid petroleum gas are considered as fuels. An experimental setup was constructed to implement the technique of laser light extinction and laser-induced incandescence. These non-intrusive techniques provide instant information, in real time, about soot volume fraction. The measurements were calibrated and validated using methods and results of studies available in the literature, and also the uncertainty is analysed and suitably minimized. The results will contribute to the database for projects of combustion systems optimization, aiming to increase the efficiency and soot emissions control.

关键词： Soot Emissions Laminar Diffusion Flames Commercial Fuels

Weather Radar Data and Distributed Hydrological modelling: An Application for mexico Valley

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Open Journal of modern Hydrology 2013年第2期3卷 79-88页

作者： Baldemar méndez-antonio Ernesto caetano Gabriel Soto-Cortés Fabián G. Rivera-Trejo Ricardo A. Carvajal Rodríguez Christopher Watts metropolitan Auto- nomous University Mexico City Mexico National Autonomous University of mexico Mexico City Mexico Sonora University Hermosillo Mexico Tabasco Autonomous Juárez University Villahermosa Mexico.

The frequent occurrence of exceptionally very heavy rainfall in mexico during the summer causes flash floods in many areas and major economic losses. As a consequence, a significant part of the annual government budget is diverted to the reconstruction of the disasters caused by floods every year, resulting hold up in the country development. A key element to mitigate the flash flood hazards is the implementation of an early warning system with the ability to process the necessary information in the shortest possible time, in order to?increase structural and non-structural resilience in flood prone regions. The real-time estimation of rainfall is essential for the implementation of such systems and the use of remote sensing instruments that feed the operational rainfall-runoff hydrological models is becoming of increasing importance worldwide. However, in some countries such as mexico, the application of such technology for operational purposes is still in its infancy. Here the implementation of an operational hydrological model is described for the mixcoac river basin as part of the non-structural measures that can be applied for intense precipitation events. The main goal is to examine the feasibility of the use of remote sensing instruments and establish a methodology to predict the runoff in real time in urban river basins with complex topography, to increase the resilience of the areas affected by annual floods. The study takes data from weather radar operated by the National meteorological Service of mexico, as input to a distributed hydrological model. The distributed unit hydrograph model methodology is used in order to assess its feasibility in urban experimental basin. The basic concepts underlying the model, as well as calibration and validation are discussed. The results demonstrate the feasibility of using weather radar data for modeling rainfall-runoff process with distributed parameter models for urban watersheds. A product resulting from this study w

关键词： Radar Distributed Hydrologic modeling Resilience

In vivo study of conductive 3D printed PCL/mWCNTs scaffolds with electrical stimulation for bone tissue engineering

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Bio-Design and manufacturing 2021年第2期4卷 190-202页

作者： Edney P.e Silva Boyang Huang Júlia V.Helaehil Paulo R.L.Nalesso Leonardo Bagne maraiara A.de Oliveira Gabriela C.C.Albiazetti Ali Aldalbahi mohamed El-Newehy milton Santamaria-Jr Fernanda A.S.mendonça Paulo Bártolo Guilherme F.caetano Postgraduate Program in Biomedical Sciences University Center of Hermínio Ometto Foundation-FHOArarasSao PauloBrazil Department of mechanical Aerospace and Civil EngineeringSchool of EngineeringUniversity of ManchesterManchester M139PLUK King Abdullah Institute for Nanotechnology King Saud UniversityRiyadh 11451Saudi Arabia

Critical bone defects are considered one of the major clinical challenges in reconstructive bone *** combination of 3D printed conductive scaffolds and exogenous electrical stimulation(ES)is a potential favorable approach for bone tissue *** this study,3D conductive scaffolds made with biocompatible and biodegradable polycaprolactone(PCL)and multi-walled carbon nanotubes(mWCNTs)were produced using the extrusion-based additive manufacturing to treat large calvary bone defects in *** results show that the use of PCL/mWCNTs scaffolds and ES contributes to thicker and increased bone tissue formation within the bone *** and mineralization are also significantly promoted using high concentration of mWCNTs(3 wt%)and ***,scaffolds favor the tartrate-resistant acid phosphatase(TRAP)positive cell formation,while the addition of mWCNTs seems to inhibit the osteoclastogenesis but present limited effects on the osteoclast functionalities(receptor activator of nuclear factor κβ ligand(RANKL)and osteoprotegerin(OPG)expressions).The use of ES promotes the osteoclastogenesis and RANKL expressions,showing a dominant effect in the bone remodeling *** results indicate that the combination of 3D printed conductive PCL/mWCNTs scaffold and ES is a promising strategy to treat critical bone defects and provide a cue to establish an optimal protocol to use conductive scaffolds and ES for bone tissue engineering.

关键词： Additive manufacturing Bone regeneration Bone remodeling Carbon nanotube Conductive scaffolds Electrical stimulation

Assessment of Undergraduate Students in Use of Hypermedia Education on Peripheral Venous Punch

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Health 2015年第4期7卷 423-429页

作者： Natasha marques Frota Lívia moreira Barros Francisco Gilberto Fernandes Pereira máguida Gomes da Silva Jennara Candido do Nascimento monaliza Ribeiro mariano Joselany áfio caetano Department of Nursing Federal University of Ceará Fortaleza Brazil

This is a qualitative study that aims to describe the opinion of nursing students on the use of an educational hypermedia on Peripheral Venipuncture. We interviewed 16 nursing students of a private university on second semester of 2014. The interviews allowed the identification of units of meaning and categorization by content analysis and led to four categories: influence of hypermedia on learning, educational technology as support to teaching classroom, contribution of hypermedia for professional practice and access difficulties. Hypermedia showed positive results as most scholars reported that the material helped in learning and generated autonomy and interactivity in the moment of studying. However some reported difficulties in access hypermedia related mainly to the server used. So hypermedia proved to be a valuable tool to use, since it favored the learning and aroused the interest and curiosity of students.

关键词： Nursing Internet Educational Technology Educational measurement Catherization Peripheral