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CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae

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Rice science 2022年第6期29卷 535-544,I0023-I0030页

作者： Fabiano T.P.KTÁVORA anne Cécile MEUNIER Aurore VERNET Murielle PORTEFAIX Joëlle MIlAZZO Henri ADREIT Didier THARREAU Octávio lFRANCO Angela MEHTA Department of Genetics and Biotechnology Federal University of Juiz de ForaJuiz de Fora-MG 36036330Brazil Embrapa Genetic Resources and Biotechnology Brasília-DF 70770-917Brazil Genetic Improvement and Adaptation of Mediterranean and Tropical Plants Agricultural Research Center for International Development(CIRAD)Montpellier 34398 CEDEX 5France CIRAD-INRAe-Agro Institute University of MontpellierMontpellier 34000France Plant Health Institute of Montpellier CIRADMontpellier 34398France University of Montpellier National Institute of Agronomic ResearchResearch Institute for DevelopmentMontpellier SupAgroMontpellier 34398France Department of Genomics Science and Biotechnology Proteomics Analysis CenterCatholic University of BrasíliaBrasília-DF 71966-900Brazil S-Inova Biotech Catholic University of Dom BoscoCampo Grande-MS 79117-900Brazil

Rice genes OsDjA2 and OsERF104,encoding a chaperone protein and an APETElA2/ethylene-responsive factor,respectively,are strongly induced in a compatible interaction with blast fungus,and also have function in plant susceptibility validated through gene ***,we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance.A total of 15 OsDjA2(62.5%)and 17 OsERF104(70.8%)T_(0)transformed lines were identified from 24 regenerated plants for each target and used in downstream *** of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area,compared with the infected control *** results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.

关键词： gene editing plant-pathogen interaction Magnaporthe pathosystem plant immunity blast resistance S-gene rice

Global transcriptional changes in response to cGAMP depend on STING in human THP-1 cells

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Cellular & Molecular Immunology 2018年第11期15卷 983-985页

作者： anne louise Hansen Aske M.Brandtoft Mette Nyegaard anne l.Thielke David Olagnier Christian K.Holm Department of Biomedicine Aarhus Research Center for Innate ImmunologyAarhus UniversityAarhusDenmark

Cytosolic detection of foreign or misplaced self-DNA through the cGAS-STING pathway leads to cGAS-mediated synthesis of the second messenger molecule *** immune signaling molecule STING binds cGAMP directly,and thus functions as a cGAMP *** of cGAMP induces the activation of STING with the subsequent induction of anti-viral and proinflammatory ***,it is not known whether STING is the only cellular sensor of cGAMP,and it has not been reported whether stimulation with cGAMP can induce gene transcription independently of *** we analyzed the global cGAMP-induced transcriptional gene program in STING-deficient human monocyte-derived THP-1 *** found that the global cGAMP-induced transcriptional response was dependent on STING and failed to identify groups of genes that were affected by cGAMP in the absence of *** data therefore strongly indicate that STING is the sole receptor for cGAMP in human macrophages.

关键词： stimulation STING transcriptional

Probability Distributions Arising in Connection with the Inspection Paradox for the Poisson Process

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Open Journal of Statistics 2023年第6期13卷 16-24页

作者： James E. Marengo anne Marino Himes W. Cade Reinberger David l. Farnsworth School of Mathematical Sciences Rochester Institute of Technology Rochester USA

The Inspection Paradox refers to the fact that in a Renewal Process, the length of the interarrival period which contains a fixed time is stochastically larger than the length of a typical interarrival period. To provide a more complete understanding of this phenomenon, conditioning arguments are used to obtain the distributions and moments of the lengths of the interarrival periods other than the one containing this fixed time for the case of the time-homogeneous Poisson Process. Distributions of the waiting times for events that occur both before and after this fixed time are derived. This provides a fairly complete probabilistic analysis of the Inspection Paradox.

关键词： Inspection Paradox Interarrival Time Poisson Process Renewal Process Waiting Time

Expert group syndrome at high altitude

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Sports Medicine and Health Science 2022年第3期4卷 215-218页

作者： Jean-Paul Richalet Marie-anne Magnan Alice Gavet Pierre lafère UniversitéSorbonne Paris Nord Laboratoire Hypoxie et PoumonUMR INSERM U1272BobignyFrance Institut National des Sports de l’Expertise et de la PerformancePôle médicalParisFrance Hôpitaux universitaires de Genève Département de médecine aiguëUnitéde médecine subaquatique et hyperbareGenevaSwitzerland Hôpitaux du Mont-Blanc Soins de suite et réadaptationChamonixFrance Universitéde Bretagne Occidentale Laboratoire ORPHYEA 44324BrestFrance HauteÉcole Bruxelles-Brabant(HE2B) EnvironmentalOccupationalAgeing(Integrative)Physiology LaboratoryBrusselsBelgium

During a training session for the university diploma of Mountain medicine delivered by University Sorbonne Paris Nord for medical doctors,one of the participants developed signs of maladaptation to high altitude at 3600 m,the severity of which was incorrectly *** was sparingly given by the patient(an anesthetist)to several of his colleagues and no one was in charge to collect clinical data,take a history,and provide appropriate *** combination of the absence of designation of a supervising doctor and the difficulty of communicating with the patient led to a lack of coordinated management and to an evolution of the symptoms towards severe acute mountain ***,the very rapid management of the patient and a rapid helicopter evacuation,as soon as the symptoms worsened towards the onset of a suspected high altitude cerebral and/or pulmonary edema,allowed rapid resolution without ***,medical,psychological,and managerial factors led to this Expert Group Syndrome.

关键词： Acute mountain sickness High altitude cerebral edema Psychology Group management

Proximal and distal esophageal sensitivity is decreased in patients with Barrett’s esophagus

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World Journal of Gastroenterology 2011年第4期17卷 514-521页

作者： anne l Kraru Sφren S Olesen Peter Funch-Jensen Hans Gregersen Asbjφrn M Drewes Mech-Sense Department of Gastroenterology Aalborg Hospital Aarhus University Hospital Department of Surgery l Aarhus University Hospital

AIM: To investigate sensations to multimodal pain stimulation in the metaplastic and normal parts of the esophagus in patients with Barrett’s esophagus (BE). METHODS: Fifteen patients with BE and 15 age-matched healthy volunteers were subjected to mechanical, thermal and electrical pain stimuli of the esophagus. Both the metaplastic part and the normal part (4 and 14 cm, respectively, above the esophago-gastric junction) were examined. At sensory thresholds the stimulation intensity, referred pain areas, and evoked brain potentials were recorded. RESUlTS: Patients were hyposensitive to heat stimulation both in the metaplastic part [median stimulation time to reach the pain detection threshold: 15 (12-34) s vs 14 (6-23) s in controls; F = 4.5, P = 0.04] and the normal part of the esophagus [median 17 (6-32) s vs 13 (8-20) s in controls; F = 6.2, P = 0.02]. Furthermore, patients were hyposensitive in the metaplastic part of the esophagus to mechanical distension [median volume at moderate pain: 50 (20-50) ml vs 33 (13-50) ml in controls; F = 5.7, P = 0.02]. No indication of central nervous system abnormalities was present, as responses were comparable between groups to electrical pain stimuli in the metaplastic part [median current evoking moderate pain: 13 (6-26) mA vs 12 (9-24) mA in controls; F = 0.1, P = 0.7], and in the normal part of the esophagus [median current evoking moderate pain: 9 (6-16) mA, vs 11 (5-11) mA in controls; F = 3.4, P = 0.07]. Furthermore, no differences were seen for the referred pain areas (P-values all > 0.3) or latencies and amplitudes for the evoked brain potentials (P-values all > 0.1). CONClUSION: Patients with BE are hyposensitive both in the metaplastic and normal part of esophagus likely as a result of abnormalities affecting peripheral nerve pathways.

关键词：食管患者敏感性控制电流远端中枢神经系统诱发电位检测阈值

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Genes & Diseases 2022年第6期9卷 1506-1520页

作者： Jon Christiansen anne-Katrin Guttsches Ulrike Schara-Schmidt Matthias Vorgerd Christoph Heute Corinna Preusse Werner Stenzel Andreas Roos Department of Pediatric Neurology Centre for Neuromuscular DisordersCentre for Translational Neuro-and Behavioral SciencesUniversity Hospital EssenUniversity Duisburg-Essen45147 EssenGermany Department of Neurology Heimer Institute for Muscle ResearchUniversity Hospital BergmannsheilRuhr University Bochum44789 BochumGermany Department of Adult and Pediatric Diagnostic Interventional RadiologyNuclear MedicineBG University Hospital Bergmannsheil Bochum44789 BochumGermany Charite´e Universitatsmedizin Berlin Freie Universitat Berlin10117 BerlinGermany Humboldt-Universitat zu Berlin Department of Neuropathology10117 BerlinGermany Department of Neurology with Institute for Translational Neurology University Hospital MunsterAlbert-Schweitzer-Campus 148149 MunsterGermany Children’s Hospital of Eastern Ontario Research Institute University of OttawaOttawaON K1H 8L1Canada

Anoctamin-5(ANO5)is a multi-pass membrane protein localized to the sarcolemma and the sarcoplasmic *** were linked to rare autosomal recessive muscle ***,we summarize the clinical spectrum,imaging data and molecular research findings as well as results of animal modeling,which significantly moved forward the understanding of mechanisms underlying ANO5-related muscle *** that precise histological information on inflammatory processes taking place in patient-derived muscle are still lacking,an(immuno)histological study on biopsies derived from six ANO5-patients was performed showing focal accumulation of necrotic fibers,mild fiber-size variances and *** addition,MRI data of four ANO5-patients(including a 10-year follow-up in one patient)are presented and discussed in the context of previously published ***,data presented in this article combining a review of the literature with own myopathological findings address scientific trends and open questions on ANO5-related muscle diseases,which would be of significant interest for a wide neuromuscular diseases *** conclude,a clear genotypeephenotype correlation does not exist,and ANO5-related muscle disorders might represent the next entity of a clinical continuum with varying degree of muscle cell *** addition,results of pre-clinical studies allowed the definition of suitable cell and animal models characterized by certain histological and functional pathologies resembling the human *** models might serve as suitable systems for testing of interventional concepts in future.

关键词： Anoctamin-5 lGMDR12 MMD3 Muscle inflammation Muscle MRI

Survey of Staphylococcus aureus in a general pediatric population and focus on isolates with three clinically relevant toxin-encoding genes

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World Journal of Pediatrics 2018年第1期14卷 35-43页

作者： anne Filleron Sarah Beauregard-Birba Thibault Mura Fabien Aujoulat anne laure Michon Michel Rodière Tu Anh Tran Eric Jeziorski Hélène Marchandin Department of Pediatrics Nimes University HospitalNimesFrance INSERM U1183 Team3Immune Regulationand Micr obiotaMedical University of Montpellier NimesNimesFrance laboratory ofBacter iology Montpellier University HospitalMontpellierFrance Department of Medical Information Montpellier UniversityHospitalMontpellierFrance Montpellier University UMR5569HydroSciencesMontpellierPharmacy University of MontpellierMontpellierFrance Division of Pediatric Infectious Diseases Departmentof P ediatricsMontpellier University HospitalMontpellierFrance Department of Micr obiology Nimes University HospitalNimesFrance

Background In children, surveys on Staphylococcus aureus have focused on specific infections, situations or strains but no study has so far given an overview on S. aureus isolation without any selection. Here, we describe the overall bacteriologi-cal and clinical characteristics of S. aureus isolation in children, with a special focus on isolates harbouringtst,sea, and/or luk-PV genes, respectively, encoding the three clinically relevant toxins: toxic shock syndrome toxin-1, enterotoxin A and Panton–Valentine leukocidin. Methods Data associated with S. aureus isolation were reviewed: isolation site, infection status,tst,sea andluk-PV genes, antimicrobial susceptibility pattern,agr typing. Results Three hundred and seventy-seven isolates retrieved from 328 children during S. aureus infection (55.2%) or colo-nisation (44.8%) were ***,sea and luk-PV genes were amplified in 14.3, 9.5 and 5.8% of the isolates, respectively. These isolates were significantly more frequently retrieved during infection (69.1%) than colonisation but differences were observed according to isolation site. Methicillin-resistance was found in 7.2% of the isolates, 78% of which harboured ≥ 1 of the targeted toxin-encoding genes. Conclusions This first comprehensive study of S. aureus in children showed S. aureus to be mainly retrieved during infection and a high rate of colonisation, not limited to the nasopharynx. Predominant infections were skin and soft tissue infections wheretst was most frequently ***-PV was most commonly detected during bone and joint infections. Isolates harbouring targeted toxin-encoding genes were significantly associated with infections but a quarter of children were asymp-tomatic carriers representing a reservoir for dissemination of isolates with virulence potency.

关键词： Colonisation Infection Pediatric Staphylococcus aureus Toxins

Effectiveness and safety of apixaban and rivaroxaban vs warfarin in patients with atrial fibrillation and chronic kidney disease

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World Journal of Nephrology 2023年第5期12卷 132-146页

作者： Sylvie Perreault laurie-anne Boivin Proulx Aurélie lenglet Ziad A Massy Marc Dorais Faculty of Pharmacy University of MontrealQuebecMontreal H3C3J7Canada Department of Cardiology Faculty of MedicineUniversity of Ottawa Heart InstituteOntarioOttawa K1Y4W7Canada Department of Pharmacy Amiens-Picardie Hospital University CenterAmiens 80000France Faculty of Pharmacy MP3CV LaboratoryUR7545University of Picardie Jules VerneAmiens 80000France Division of Nephrology University of Paris Ouest-Versailles-Saint-Quentin-en-Yvelines(UVSQ)VillejuifFrance.AP-HP Ambroise-ParéHospitalBoulogne Billancourt/Paris 92104France StatSciences Inc. Notre-Dame-de-l’Île-PerrotQuebecMontreal J7W 3K8Canada

BACKGROUND Randomized controlled trials(RCTs)of direct oral anticoagulants(DOACs)included a low proportion of atrial fibrillation(AF)patients with chronic kidney disease(CKD),and suggested that DOACs are safe and effective in patients with mild-to-moderate *** a metanalysis of RCTs and observational studies,DOACs were associated with better efficacy(vs warfarin)in early CKD and had similar efficacy and safety profiles in patients with stages IV-V *** few studies have provided data on the safety and effectiveness of each DOAC vs warfarin in patients with stage III *** effectiveness and safety of DOACs in those patients are still subject to *** To assess and compare the effectiveness and safety of apixaban and rivaroxaban vs warfarin in this patient *** A cohort of patients with an inpatient or outpatient code for AF and stage III CKD who were newly prescribed apixaban and rivaroxaban was created using the administrative databases from the Quebec province of Canada between 2013 and *** primary effectiveness outcome was a composite of ischemic stroke,systemic embolism,and death,whereas the primary safety outcome was a composite of major bleeding within a year of DOAC vs warfarin *** groups were compared in an under-treatment analysis using inverse probability of treatment weighting and Cox proportional *** A total of 8899 included patients filled out a new oral anticoagulation therapy claim;3335 for warfarin and 5564 for *** with warfarin,15 mg and 20 mg rivaroxaban presented a similar effectiveness and safety composite *** 5.0 mg was associated with a lower effectiveness composite risk[Hazard ratio(HR)0.76;95%confidence interval(CI):0.65-0.88]and a similar safety risk(HR 0.94;95%CI:0.66-1.35).Apixaban 2.5 mg was associated with a similar effectiveness composite(HR 1.00;95%CI:0.79-1.26)and a lower safety risk(HR 0.65;95%CI:***,apixaban 5.0 mg was associated with a bet

关键词： Atrial fibrillation Chronic kidney disease Direct oral anticoagulant Effectiveness Safety Warfarin

Source of inorganic N affects the cost of growth in a legume tree species(Virgilia divaricata)from the Mediterrean-type Fynbos ecosystem

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Journal of Plant Ecology 2016年第6期9卷 752-761页

作者： Anathi Magadlela María A.Pérez-Fernández Aleysia Kleinert léanne l.Dreyer Alex J.Valentine Botany and Zoology Department University of StellenboschPrivate Bag X1Matieland 7602South Africa Deparment of Physical Chemical and Natural SystemsUniversidad Pablo de OlavideCarretera de Utrera Km 141013 SevilleSpain

Aims In Mediterranean-type ecosystem,the Cape Fynbos,legumes may be able to switch between soil N and atmospheric N_(2) sources during growth to adjust the carbon costs of N *** study investigated the utilization of different inorganic N sources by Virgilia divaricata,a native legume from the Mediterranean-type ecosystem of the Cape Floristic *** Plants were cultivated in sterile quartz sand,supplied with 25%strength long Ashton nutrient solution,modified to contain 500μM *** the phosphate level(500μM),plants were treated with 500μM NH_(4)NO_(3)(treatment named N),or grown in N-free nutrient solution and inoculated with effective Burkholderia sp.(Bact.)or treated with combined N sources(500μM NH_(4)NO_(3))and inoculated with effective Burkholderia sp.(N+Bact.).Important Findings The application of NH_(4)NO_(3) to the legumes resulted in a greater increase in plant dry *** construction costs were higher in plants that were supplied with mineral and symbiotic N *** photosynthetic rates per leaf area was maintained,irrespective of the N *** the plant roots were nodulated,the plant dependence on N_(2) fixation decreased with addition of *** and nodules of the plants solely reliant on N_(2) fixation showed an increase in glutamine *** results show that *** is highly adapted for growth at the forest *** and possibly anthropic soils by utilizing both atmospheric and soil N sources.

关键词： phosphate deficiency Virgilia divaricata N_(2)fixation mineral N C construction costs asparagine