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Analytical Modelling of Fuel Cells Har/Cdr

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Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces

2010年

作者： andrei A. Kulikovsky

ISBN: (数字)9780444535610

ISBN: (纸本)9780444535603

In fuel cell research, the gap between fundamental electrochemical processes and the engineering of fuel cell systems is bridged by the physical modelling of fuel cells. This relatively new discipline aims to understand the basic transport and kinetic phenomena in a real cell and stack environment, paving the way for improved design and performance. The author brings his unique approach to the analytical modeling of fuel cells to this essential reference for energy technologists.

关键词： Fuel cellsMathematical models.

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Science China(Life Sciences) 2020年第7期63卷 1053-1062页

作者： Yawei Zhao Jinzhong Tian Guosong Zheng Jun Chen Chuanwen Sun Zhongyi Yang andrei A.Zimin Weihong Jiang Zixin Deng Zhijun Wang Yinhua Lu College of Life Sciences.Shanghai Normal University Shanghai 200234China State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai 200030China Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China School of Life Science Taizhou UniversityTaizhou 318000China Institute of Biochemistry and Physiology of Microorganisms Russian Academy of SciencesPushchino 142290Russia Shanghai Engineering Research Center of Plant Germplasm Resources College of Life SciencesShanghai Normal UniversityShanghai 200234China

CRISPR/Cas-mediated genome editing has greatly facilitated the study of gene function in Streptomyces. However, it could not be efficiently employed in streptomycetes with low homologous recombination(HR) ability. Here, a deaminase-assisted base editor d Cas9-CDA-ULstr was developed in Streptomyces, which comprises the nuclease-deficient Cas9(dCas9), the cytidine deaminase from Petromyzon marinus(PmCDA1), the uracil DNA glycosylase inhibitor(UGI) and the protein degradation tag(LVA tag). Using d Cas9-CDA-ULstr , we achieved single-, double-and triple-point mutations(cytosine-to-thymine substitutions)at target sites in Streptomyces coelicolor with efficiency up to 100%, 60% and 20%, respectively. This base editor was also demonstrated to be highly efficient for base editing in the industrial strain, Streptomyces rapamycinicus, which produces the immunosuppressive agent rapamycin. Compared with base editors derived from the cytidine deaminase rAPOBEC1, the PmCDA1-assisted base editor dCas9-CDA-ULstr could edit cytosines preceded by guanosines with high efficiency, which is a great advantage for editing Streptomyces genomes(with high GC content). Collectively, the base editor dCas9-CDA-ULstr could be employed for efficient multiplex genome editing in Streptomyces. Since the d Cas9-CDA-ULstr -based genome editing is independent of HR-mediated DNA repair, we believe this technology will greatly facilitate functional genome research and metabolic engineering in Streptomyces strains with weak HR ability.

关键词： base editors dCas9-CDA-ULstr multiplex editing Streptomyces

The influence on biotissue laser resection of a strongly absorbing layer at the optical fiber tip

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 15-21页

作者： Daria Kuznetsova Vadim Elagin Maria Karabut Maria Shakhova Vladimir Bredikhin Liudmila Snopova andrei Shakhov Natalia Sapogova Nikita Bityurin Victor Bagratashvili Vladislav Kamensky Institute of Biomedical Technologies Nizhny Novgorod State Medical Academy 10/1 Minin and Pozharsky Sq. Nizhny Novgorod 603005Russia Lobachevsky State University of Nizhni Novgorod 23 Gagarin Avenue BLDG 2 Nizhny Novgorod 603950Russia Nizhny Novgorod State Medical Academy 10/1 Minin and Pozharsky Sq. Nizhny Novgorod 603005Russia Institute of Applied Physics Russian Academy of Sciences 46 Ul'yanov StreetNizhny Novgorod 603950Russia Institute on Laser and Information Technologies Russian Academy of Sciences 2 Pionerskaya St. Troitsk 142092Russia

In this paper,we consider a method of laser resection using the silica glass core from which the cladding layer has been removed as the cutting part of a laser *** absorbing layer coating the silica fiber tip markedly alters its biotissue cutting character *** results of histological studies of skin after exposure to a laser scalpel with and without a strongly absorbing coating(SAC)at a wavelength of 097μm show that resection using a coated scalpel is more *** an uncoated scalpel was used,skin injury was more apparent in both its surface spread and the depth of structural damage,resulting in poorer tissue regeneration.

关键词： Laser surgery silica fibers strongly absorbing coating histology

Geometric Aspects of Quasi-Periodic Property of Dirichlet Functions

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Advances in Pure Mathematics 2018年第8期8卷 699-710页

作者： Dorin Ghisa andrei Horvat-Marc Department of Mathematics York University Glendon College Toronto Canada North University Center at Baia Mare Faculty of Science Department of Mathemathics and Computer Science Technical University of Cluj Napoca Baia Mare Romania

The concept of quasi-periodic property of a function has been introduced by Harald Bohr in 1921 and it roughly means that the function comes (quasi)-periodically as close as we want on every vertical line to the value taken by it at any point belonging to that line and a bounded domain Ω. He proved that the functions defined by ordinary Dirichlet series are quasi-periodic in their half plane of uniform convergence. We realized that the existence of the domain Ω is not necessary and that the quasi-periodicity is related to the denseness property of those functions which we have studied in a previous paper. Hence, the purpose of our research was to prove these two facts. We succeeded to fulfill this task and more. Namely, we dealt with the quasi-periodicity of general Dirichlet series by using geometric tools perfected by us in a series of previous projects. The concept has been applied to the whole complex plane (not only to the half plane of uniform convergence) for series which can be continued to meromorphic functions in that plane. The question arise: in what conditions such a continuation is possible? There are known examples of Dirichlet series which cannot be continued across the convergence line, yet there are no simple conditions under which such a continuation is possible. We succeeded to find a very natural one.

关键词： Dirichlet Functions Analytic Continuation Fundamental Domains Quasi-periodic Functions

Structure-property relationships in ILs: A study of the alkyl chain length dependence in vaporisation enthalpies of pyridinium based ionic liquids

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Science China Chemistry 2012年第8期55卷 1525-1531,1-6页

作者： ZAITSAU Dzmitry H. YERMALAYEU andrei V. EMEL'YANENKO Vladimir N. VEREVKIN Sergey P. WELZ-BIERMANN Urs SCHUBERT Thomas Department of Physical Chemistry University of Rostock Dr-Lorenz-Weg 1 D-18059 Rostock Germany Evonik Degussa Taiwan Ltd. 133 Minsheng East Road Section Taipei 10596 China 3IoLiTec Ionic Liquids Technologies GmbH Salzstrasse 184 74076 Heilbronn Germany

The enthalpies of vaporization for the series of pyridinium-based ionic liquids with bis（trifluoromethylsulfonyl）imide anion [CnPy][NTfz] （n = 2, 3, 4, 5, and 6） have been determined with the quartz crystal microbalance technique combined with the Langmuir evaporation. The linear dependence of vaporization enthalpies on the chain length has been revealed. New approach based on volumetric, surface tension, and speed of sound measurements has been developed for estimation of heat capacity differences between gas and liquid phase, which were required for adjustment of measured vaporization enthalpies to the ref- erence temperature 298 K.

关键词： ionic liquids enthalpy of vaporization quartz crystal microbalance structure-property relations

Methods of the Restoration of Wooden Architectural Monuments in Russia

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Methods of the Restoration of Wooden Architectural Monuments...

Prostitutes’Congress Teaches to Forget Shame

作者： andrei Bode Scientific Research Institute of the Theory and History of Architecture and Urban Planning Branch of the Central Scientific-Research and Project Institute of the Construction Ministry of Russia Federal State Budget Educational Institution of Higher Education "National Research Moscow State University of Civil Engineering"(Nru Mgsu)

Monuments of wooden architecture constitute a significant and valuable part of the architectural heritage of Russia. Work on their preservation has its own specifics, due to the material and its properties. The article deals with the methods of restoration of historical wooden buildings. The main trends in the Russian restoration practice are noted. Examples illustrating various restoration methods and approaches are analyzed. The advantages and disadvantages of each of the considered restoration methods for wooden objects are revealed. The criteria for assessing the quality of implemented restoration solutions are the accuracy and authenticity. Restoration correct results on the majority of monuments of wooden architecture give the application of an analytical method.

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当代外语研究 2003年第1期 12-14页

作者： andrei Khalip 章谊静

选注者言:我在网上读到此文,又让我想起在巴西工作和生活的10年。娼妓,是一种丑恶的社会现象,即使是在西方,人们也会对此职业嗤之以鼻。本文所提到的妓女代表大会居然要让从事卖淫者忘却羞耻,这倒让人想起了“此地无银三百两”的古训。... 详细信息

选注者言:我在网上读到此文,又让我想起在巴西工作和生活的10年。娼妓,是一种丑恶的社会现象,即使是在西方,人们也会对此职业嗤之以鼻。本文所提到的妓女代表大会居然要让从事卖淫者忘却羞耻,这倒让人想起了“此地无银三百两”的古训。更又甚者,一位妓女甚至狂言:I consider myself a qualified shrink(精神病医生).贵刊有“天下奇观”的专栏,我选注此文,不知能用否。

关键词：精神病 der Congress Teaches to Forget Shame Prostitutes

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粒子物理学与暴胀宇宙学(上)

大学物理 1990年第8期 38-42页

作者： andrei Linde

暴胀宇宙与粒子物理学的合流是现代物理学前沿中引人入胜的新进展．林德（Ａ．Ｌｉｎｄｅ）是这一崭新研究领域的开拓者．他撰写的此文对于这一内容浩瀚、难度很大的主题作了深入浅出的介绍．现由大连海军舰艇学院的王志珪同志将此文... 详细信息

暴胀宇宙与粒子物理学的合流是现代物理学前沿中引人入胜的新进展．林德（Ａ．Ｌｉｎｄｅ）是这一崭新研究领域的开拓者．他撰写的此文对于这一内容浩瀚、难度很大的主题作了深入浅出的介绍．现由大连海军舰艇学院的王志珪同志将此文译出，介绍给读者．因文章较长，拟分两期刊出．

关键词：密度粒子物理学量子涨落度规高能物理学宇宙理论暴胀宇宙数量级基本粒子理论

Time-resolved terahertz spectroscopy of charge carrier dynamics in the chalcogenide glass As_(30)Se_(30)Te_(40)[Invited]

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Photonics Research 2016年第3期4卷 22-28页

作者： Tianwu Wang Elena A.Romanova Nabil Abdel-Moneim David Furniss Anna Loth Zhuoqi Tang Angela Seddon Trevor Benson andrei Lavrinenko Peter Uhd Jepsen Department of Photonics Engineering (DTU Fotonik) Technical University of Denmark Saratov State University Department of Physics Faculty of Engineering University of Nottingham

Broadband(1.6–18 THz) terahertz time-domain spectroscopy(THz-TDS) and time-resolved terahertz spectroscopy(TRTS) were performed on a 54 μm thick chalcogenide glass(As_(30)Se_(30)Te_(40)) sample with a two-color laser-induced air plasma THz system in transmission and reflection modes, respectively. Two absorption bands at 2–3 and 5–8 THz were observed. TRTS reveals an ultrafast relaxation process of the photoinduced carrier response, well described by a rate equation model with a finite concentration of mid-bandgap trap states for self-trapped *** photoinduced conductivity can be well described by the Drude–Smith conductivity model with a carrier scattering time of 12–17 fs, and we observe significant carrier localization effects. A fast refractive index change was observed 100 fs before the conductivity reached its maximum, with 2 orders of magnitude larger amplitude than expected for the optically induced THz Kerr effect, indicating that free carriers are responsible for the transient index change.

关键词： Te Invited Se Time-resolved terahertz spectroscopy of charge carrier dynamics in the chalcogenide glass As