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The High Light Response and Redox Control of Thylakoid FtsH Protease in Chlamydomonas reinhardtii

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Molecular Plant 2017年第1期10卷 99-114页

作者： Fei Wang Yafei Qi Alizee Malnoe Yves Choquet Francis-andre Wollman Institut de Biologie Physico-Chimique Unite Mixte de Recherche 7141 Centre National de la Recherche Scientifique/Universite Pierre et Marie Curie Paris 75005 France

In Chlamydomonas reinhardtii, the major protease involved in the maintenance of photosynthetic machinery in thylakoid membranes, the FtsH protease, mostly forms large hetero-oligomers （-1 MDa） comprising FtsH1 and FtsH2 subunits, whatever the light intensity for growth. Upon high light exposure, the FtsH subunits display a shorter half-life, which is counterbalanced by an increase in FTSH1/2 mRNA levels, resulting in the modest upregulation of FtsH1/2 proteins. Furthermore, we found that high light increases the protease activity through a hitherto unnoticed redox-controlled reduction of intermolecular disulfide bridges. We iso- lated a Chlamydomonas FTSH1 promoter-deficient mutant, ftsh1-3, resulting from the insertion of a TOC1 transposon, in which the high light-induced upregulation of FTSH1 gene expression is largely lost. In ftsh1- 3, the abundance of FtsH1 and FtsH2 proteins are loosely coupled （decreased by 70% and 30%, respectively） with no formation of large and stable homo-oligomers. Using strains exhibiting different accumulation levels of the FtsH1 subunit after complementation of ftsh1-3, we demonstrate that high light tolerance is tightly correlated with the abundance of the FtsH protease. Thus, the response of Chlamydomonas to light stress involves higher levels of FtsH 1/2 subunits associated into large complexes with increased proteolytic activity.

关键词： chloroplast protease regulation of gene expression photoinhibition Chlamydomonas reinhardtii

Bone marrow cells produce nerve growth factor and promote angiogenesis around transplanted islets

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World Journal of Gastroenterology 2010年第10期16卷 1215-1220页

作者： Naoaki Sakata Nathaniel K Chan John Chrisler andre Obenaus Eba Hathout Islet Transplant Laboratory Department of PediatricsLoma Linda University School of Medicine Division of Hepato-Biliary Pancreatic Surgery Department of SurgeryTohoku University Graduate School of Medicine Departments of Radiation Medicine and Radiology Loma Linda University School of Medicine

AIM:To clarify the mechanism by which bone marrow cells promote angiogenesis around transplanted ***: Streptozotocin induced diabetic BALB/ c mice were transplanted syngeneically under the kidney capsule with the following: (1) 200 islets (islet group: n=12), (2) 1-5×106 bone marrow cells (bone marrow group: n=11), (3) 200 islets and 1-5×106 bone marrow cells (islet + bone marrow group: n= 13), or (4) no cells (sham group:n=5). All mice were evaluated for blood glucose, serum insulin, serum nervegrowth factor (NGF) and glucose tolerance (GTT) up to postoperative day (POD) 14. Histological assessment for insulin, von Willebrand factor (vWF) and NGF was performed at POD 3, 7 and ***: Blood glucose level was lowest and serum insulin was highest in the islet + bone marrow group. Serum NGF increased in islet, bone marrow, and islet + bone marrow groups after transplantation, and there was a significant difference (P=0.0496, ANOVA) between the bone marrow and sham groups. The number of vessels within the graft area was signif icantly increased in both the bone marrow and islet + bone marrow groups at POD 14 as compared to the islet alone group (21.2 ± 3.6 in bone marrow, P=0.01, vs islet group, 22.6 ± 1.9 in islet + bone marrow, P = 0.0003, vs islet group, 5.3 ± 1.6 in islet-alone transplants). NGF was more strongly expressed in bone marrow cells compared with islets. CONCLUSION: Bone marrow cells produce NGF and promote angiogenesis. Islet co-transplantation with bone marrow is associated with improvement of islet graft function.

关键词： Islet transplantation Bone marrow cells Nerve growth factor Angiogenesis Endothelial precursor cells

Mechanical properties characterization of different types of masonry infill walls

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Frontiers of Structural and Civil Engineering 2020年第2期14卷 411-434页

作者： andre FURTADO Hugo RODRIGUES Antonio AREDE Humberto VARUM CONSTRUCT-LESE Department of Civil EngineeringFaculty of EngineeringUniversity of PortoPorto 4200-465Portugal R1SCO School of Technology and ManagementPolytechnic Institute of LeiriaLeiria 2411-901Portugal

It is remarkable,the recent advances concerning the development of numerical modeling frameworks to simulate the infill panels'seismic ***,there is a lack of experimental data of their mechanical properties,which are of full importance to calibrate the numerical *** primary objective of this paper is to present an extensive experimental campaign of mechanical characterization tests of infill masonry walls made with three different types of masonry units:lightweight vertical hollow concrete blocks and hollow clay *** different types of experimental tests were carried out,namely:compression strength tests,diagonal tensile strength tests,and flexural strength tests parallel and perpendicular to the horizontal bed joints.A total amount of 80 tests were carried out and are reported in the present *** second objective of this study was to compare the mechanical properties of as-built and existing infill *** results presented and discussed herein,will be in terms of strain-stress curves and damages observed within the *** was observed a fragile behavior in the panels made with hollow clay horizontal bricks,without propagation of *** plaster increased the flexural strength by 57%.

关键词： masonry infill walls experimental characterization compression strength shear diagonal strength flexural strength

Influencing Factors in Partnership Management of Brazilian Small and Medium-sized Trade Enterprises Importing from China

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Influencing Factors in Partnership Management of Brazilian S...

作者： andre Pasini Herranz 上海交通大学

学位级别：硕士

Brazil and China share mutual economic development for international business and *** several years of this successful trading relationship have been strengthened by the creation of the economic group BRICS,which has allowed China to become Brazil’s largest trade partner since 2009,surpassing the United *** and mature buyer-supplier partnerships are essential for any type of trade *** have shown that there are specific factors influencing partnership management among different types of *** on that,this thesis aims to answer what are the main influencing factors in partnership management of Brazilian small and mediumsized trade enterprises（SMTEs）.These factors are commonly used to build and sustain successful partnerships with suppliers while importing from *** implication that this thesis aims to answer is how the different combinations of these factors are used to compose different partnership *** a significant number of studies have been writing about factors influencing the adoption of supply chain management practices,the empirical evidence of the main influencing factors in partnership management along a supply chain has been ***,studies are limited to other countries and industries rather than Brazil and the Electronic/Machinery *** on an extensive literature review on partnership management,its main influencing factors,and in the theories of supply chain management practices,this thesis brings an empirical test of a partnership model used to evaluate different influencing factors,classifying partnerships in different *** empirical test of the model was made through a questionnaire distributed to a selected group of Brazilian SMTEs currently importing from *** results indicate that a set of eight main influencing factors are present in every partnership,but the application level of each factor differs from company to *** combinations of these factors and le

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RasGRP Ras guanine nucleotide exchange factors in cancer

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Frontiers in Biology 2013年第5期8卷 508-532页

作者： Olga KSIONDA andre LIMNANDER Jeroen P. ROOSE Department of Anatomy University of California San Francisco San Francisco CA 94143 USA

RasGRP proteins are activators of Ras and other related small GTPases by the virtue of functioning as guanine nucleotide exchange factors （GEFs）. In vertebrates, four RasGRP family members have been described. RasGRP-1 through -4 share many structural domains but there are also subtle differences between each of the different family members. Whereas SOS RasGEFs are ubiquitously expressed, RasGRP proteins are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. Most studies have concentrated on the role of RasGRP proteins in the development and function of immune cell types because of the predominant RasGRP expression profiles in these cells and the immune phenotypes of mice deficient for Rasgrp genes. However, more recent studies demonstrate that RasGRPs also play an important role in tumorigenesis. Examples are skin- and hematological- cancers but also solid malignancies such as melanoma or prostate cancer. These novel studies bring up many new and unanswered questions related to the molecular mechanism of RasGRP-driven oncogenesis, such as new receptor systems that RasGRP appears to respond to as well as regulatory mechanisms for RasGRP expression that appear to be perturbed in these cancers. Here we will review some of the known aspects of RasGRP biology in lymphocytes and will discuss the exciting new notion that RasGRP Ras exchange factors play a role in oncogenesis downstream of various growth factor receptors.

关键词： Ras signaling lymphocytes cancer RasGRP receptor

Local Weather Events:Stories of Pedagogical Practice as Possible Cultures of Exploration

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ECNU Review of Education 2019年第4期2卷 421-440页

作者： Elin EriksenØdegaard andre Steenbuch Marandon KINDknow Resercah Center Western Norway University of Applied SciencesInndalsveien 285063 BergenNorway Kunstpilotene

Purpose:This article aims to describe and discuss what local weather landscapes mean to children and how weather implies exploring bodily sensations and *** does so by following the work of a community artist,working as a kindergarten teacher,over 1 ***/Approach/Methods:Through a narrative inquiry approach,which also includes studies of archival data and field notes,we analyze how local and personally experienced weather events imply what we call“cultures of exploration”in institutional *** epistemologies cross the specter of cultural–historical,pragmatic,and deep ecological ***:Through this study,we exemplify how experiencing weather is intertwined into pedagogical practices like habituating the body to cope with cold and wet weather,learning about danger in a wild natural landscape,and valuing species as a powerful *** descriptions exemplify“cultures of exploration”as a pedagogical ***/Value:In this time of an increasing climate crisis on our planet,the value of our findings is to foreground new insights,awareness,and knowledge relevant to children;to early-childhood education;and to life and societies at *** can thus develop methods to better care for,protect,and educate *** article has the potential to show how weather events are intertwined with everyday institutional practices—as well as how children,through exploration,learn to cope with seasonal weather landscapes and local cultural adaptations.

关键词： Cultures of exploration narrative inquiry pedagogical practice weather events weather landscapes

Hydraulic energy indices reveal spatial dependence in a subtropical soil under maize crop in Southern Brazil

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Pedosphere 2021年第5期31卷 771-782页

作者： Maria Eliza TUREK Robson andre ARMINDO Ole WENDROTH Graduate Program of Environmental Engineering(PPGEA) Federal University of Paraná(UFPR)Curitiba 81531980(Brazil) Department of Physics(DFI) Federal University of Lavras(UFLA)Mailbox 3037Lavras 37200900(Brazil) Department of Plant&Soil Sciences University of Kentucky(UK)Lexington 40546-0091(USA)

Soil physical quality(SPQ)assessment is an important part in the evaluation of soil use,management,and *** can be assessed using several physical properties,hydraulic indices,and *** from tropical and temperate regions represent different physical behaviors,and the quantification of their physical properties is important to support soil evaluation and *** objective of this study was to evaluate the SPQ in a subtropical field under maize crop cultivation according to its physical properties,hydraulic indices,and functions in an attempt to infer the spatial variability and to determine the behavior of soil physical structure across a spatial *** used soil key physical variables,such as texture,bulk density,total porosity,saturated hydraulic conductivity,and organic carbon content,were measured in a regular grid with a soil sampling density of 30 points per hectare,covering an area of0.5 *** hydraulic conductivity varied strongly between subsamples and in the field,suggesting the heterogeneity of the soil structure regarding water *** physical variables were combined with other indicators,which were based on the soil water retention curve and the pore size distribution(PSD)*** analysis was performed to verify the relationship between the measured and calculated variables,and some strong linear correlations were revealed,such as between aeration energy index and microporosity(r=0.608)and water retention energy index with microporosity(r=0.532)and with bulk density(r=0.541).For most sampled locations,the shape and location parameters of PSD showed results outside of the optimum ranges,whereas the hydraulic energy indices and cumulative hydraulic energy functions presented values that were similar to those found for some tropical soils described in the *** spatial variability of these indices was described using semivariograms and kriged maps,indicating the variability of the SPQ i

关键词： geostatistics integral energy pore size distribution soil physical indicators soil physical quality soil water retention curve

Correlation of the Rutgeerts score and recurrence of Crohn’s disease in patients with end ileostomy

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Gastroenterology Report 2017年第4期5卷 271-276,I0001,I0002页

作者： Vasutakarn Chongthammakun andre Fialho andrea Fialho Rocio Lopez Bo Shen Department of Internal Medicine The Cleveland Clinic FoundationClevelandOHUSA Department of Quantitative Health Sciences The Cleveland Clinic FoundationClevelandOHUSA The Center for Inflammatory Bowel Diseases The Cleveland Clinic FoundationClevelandOHUSA

Background:Recurrence of Crohn’s disease(CD)can occur after surgery,including end ileostomy(EI).The Rutgeerts score(RS)was developed to predict postsurgical CD recurrence via ileocolonoscopy in patients having ileocolonic *** role of ileoscopic evaluation via stoma for assessing recurrence of CD has not been *** aim of this study was to evaluate the role of ileoscopy for predicting disease recurrence in CD patients after EI with the use of ***:A total of 73 eligible CD patients with at least two ileoscopies in our institution following EI were *** inflammation of the neo-terminal ileum was graded based on the *** primary outcomes were the need for endoscopic stricture dilation and subsequent surgery due to recurrence of *** secondary outcomes were CDrelated hospitalization and the need to escalate CD-associated ***:The median duration of CD until EI was 9 years(interquartile range:4-13 years),and themedian duration from EI to the first ileoscopy was 28months(interquartile range:11-93 months).The RSs in the neo-terminal ileum close to EI were calculated,and subjects were divided into two groups:the normal RS group with the score being zero(n=25)and the abnormal RS group with the RS score being(n=48).Patients in the abnormal RS group weremore likely to have recurrence of CD(92%vs 27%)and need endoscopic dilation of stricture(40%vs 10%),subsequent bowel surgery(68%vs 15%),disease-related hospitalizations(80%vs 23%)and escalation of CDmedications(64%vs 25%)than those in the normal RS ***-to-event analysis showed that patients in the abnormal RS group were at a higher risk of endoscopic dilation(odds ratio(OR)=1.5;95%CI:1.09–1.9),need of second bowel surgery(OR=1.5;95% CI:1.2–1.8)and disease-related hospitalizations(OR=1.3;95% CI:1.1–1.6)after adjusting for factors such as duration from surgery to sensor,duration of disease and the patient’s sex(all P<0.001).Further multivariable analysis showed

关键词： Crohn’s disease Rutgeerts score ileoscopy ileostomy recurrence

To Open the Mouth,to Show the Tongue:Anthropophagic Gestures in Brazilian Art

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Journal of Foreign Languages and Cultures 2021年第2期5卷 143-151,155,156页

作者： Eduardo Jorge de Oliveira andre Masseno University of Zurich

This essay seeks to articulate a detailed reading through two anatomical selections-the mouth and tongue-in Brazilian art from the end of the *** reading is part of a shift in the matrix of Oswald de Andrade’s“Anthropophagic Manifesto,”originally published in the Re vista de Antropofagia in 1928,whose interpretations spread in Brazil during the second half of the 20th *** study mobilises elements of the manifesto and its resonance in the works of artists such as Anna Maria Maiolino,Lygia Pape,Paulo Bruscky,and Lenora de Barros.

关键词： mouth tongue Anthropophagic Manifesto Oswald de Andrade Brazilian art