检索结果-南通市图书馆

Marine Life Science & Technology 2020年第3期2卷 246-251页

作者： andréluiz veiga conrado Renata Stecca lunes Carlos Eduardo Malavasi Bruno Aline Tiemi Shiraishi Rocha JoséRoberto Machado Cunha da Silva Laboratory of Evolutive Histophysiology Department of Cell and Developmental BiologyInstitute of Biomedical SciencesUniversity of Sao PauloAvenida ProfLineu Prestes1524Cidade UniversitariaSao Paulo 05508-000Brazil Laboratory of Immunological Techniques Applied to Morphophysiology School of Veterinary Medicine and Animal ScienceDepartment of SurgeryUniversity of Sao PauloAvenida Prof.Di.Orlando Marques de Paiva87.Cidade UniversitariaSao Paulo 05508-270Brazil

Remoras are commensal fish of various marine species,such as sharks,swordfishes,turtles,dolphins,manta rays and *** specimen of marlin sucker Remora osteochir was evaluated using computed tomography for skeletal digital reconstruction,digital radiology for general evaluation and bone counting,and the double contrast technique to distinguish coelomic *** radiographic images,it was possible to observe otoliths in the center of the neurocranium,to count 27 vertebrae,nine pairs of ribs,and to detect the presence of the hipural and epural bones near of the caudal *** double contrast images,it was possible to visualize the swim bladder,gills,heart,liver,stomach and *** the tomographic images and reconstructions,it was possible to identify the intercalar bones of the cephalic disc;the spine with vertebral bodies composed of neural arches and ventral ribs;and the pectoral girdle formed by posttemporal,cleithrum and scapulocoracoid *** was concluded that digital radiology and computed tomography were able to describe anatomical structures of marlin sucker *** without the need for dissection.

关键词： Teleost fish Double contrast Computed tomography Digital radiology Skeleton

Junctional epithelium and hemidesmosomes:Tape and rivets for solving the“percutaneous device dilemma”in dental and other permanent implants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第12期7卷 178-198页

作者： Nicholas G.Fischer conrado Aparicio MDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics University of Minnesota16-212 Moos Tower515 Delaware St.SEMinneapolisMN55455USA Division of Basic Research Faculty of OdontologyUIC Barcelona-Universitat Internacional de CatalunyaC/.Josep Trueta s/n08195Sant Cugat del VallesBarcelonaSpain Institute for Bioengineering of Catalonia(IBEC) The Barcelona Institute of Science and Technology(BIST)C/.Baldiri Reixac 10-1208028BarcelonaSpain

The percutaneous device dilemma describes etiological factors, centered around the disrupted epithelial tissue surrounding non-remodelable devices, that contribute to rampant percutaneous device infection. Natural percutaneous organs, in particular their extracellular matrix mediating the “device”/epithelium interface, serve as exquisite examples to inspire longer lasting long-term percutaneous device design. For example, the tooth’s imperviousness to infection is mediated by the epithelium directly surrounding it, the junctional epithelium (JE). The hallmark feature of JE is formation of hemidesmosomes, cell/matrix adhesive structures that attach surrounding oral gingiva to the tooth’s enamel through a basement membrane. Here, the authors survey the multifaceted functions of the JE, emphasizing the role of the matrix, with a particular focus on hemidesmosomes and their five main components. The authors highlight the known (and unknown) effects dental implant - as a model percutaneous device - placement has on JE regeneration and synthesize this information for application to other percutaneous devices. The authors conclude with a summary of bioengineering strategies aimed at solving the percutaneous device dilemma and invigorating greater collaboration between clinicians, bioengineers, and matrix biologists.

关键词： Percutaneous device Percutaneous implant Hemidesmosome Junctional epithelium Bioinspired surfaces

Injection of bone marrow mesenchymal stem cells by intravenous or intraperitoneal routes is a viable alternative to spinal cord injury treatment in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2018年第6期13卷 1046-1053页

作者： Bruna dos Santos Ramalho FernANDa Martins de Almeida conrado Mendonca Sales Silmara de Lima Ana Maria Blanco Martinez Laboratorio de Neurodegeneracao e Reparo Departamento de Patologia - Faculdade de Medicina HUCFF Universidade Federal do Rio de Janeiro Rio de laneiro Brazil

In spite of advances in surgical care and rehabilitation, the consequences of spinal cord injury （SCI） are still challenging. Several experimental therapeutic strategies have been studied in the SCI field, and recent advances have led to the development of therapies that may act on the inhibitory microenvironment. Assorted lineages of stem cells are considered a good treatment for SCI. This study investigated the effect of systemic transplantation of mesenchymal stem cells （MSCs） in a compressive SCI model. Here we present results of the intraperitoneal route, which has not been used previously for MSC administration after compressive SCI. We used adult female C57BL/6 mice that underwent laminectomy at the T9 level, followed by spinal cord compression for 1 minute with a 30-g vascular clip. The animals were divided into five groups： sham （anesthesia and laminectomy but without compression injury induction）, MSC i.p. （intraperitoneal injection of 8×10^5 MSCs in 500 μL of DMEM at 7 days after SCI）, MSC i.v. （intravenous injection of 8 × 10^5 MSCs in 500μL of DMEM at 7 days after SCI）, DMEM i.p. （intraperitoneal injection of 500μL of DMEM at 7 days after SCI）, DMEM i.v. （intravenous injection of 500 μL of DMEM at 7 days after SCI）. The effects of MSCs transplantation in white matter sparing were analyzed by luxol fast blue staining. The number of preserved fibers was counted in semithin sections stained with toluidine blue and the presence of trophic factors was analyzed by immunohistochemistry. In addition, we analyzed the locomotor performance with Basso Mouse Scale and Global Mobility Test. Our results showed white matter preservation and a larger number of preserved fibers in the MSC groups than in the DMEM groups. Furthermore, the MSC groups had higher levels of trophic factors （brain-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and neurotrophin-4） in the spinal cord and improved locomotor performance. Our results indicate that injection of MSC

关键词： spinal cord injury mesenchymal stem cells intravenous route intraperitoneal route functional recovery systemic transplantation neural regeneration

Heterostructure engineering in electrode materials for sodium-ion batteries:Recent progress and perspectives

在线全文

学校读者我要写书评

暂无评论

eScience 2023年第5期3卷 1-11页

作者： Eric Gabriel Chunrong Ma Kincaid Graff Angel conrado Dewen Hou Hui Xiong Micron School of Materials Science and Engineering Boise State UniversityBoiseID83725USA X-ray Sciences Division Argonne National LaboratoryLemontIL60439USA College of Textiles&Clothing Qingdao UniversityQingdao266071China Key Laboratory of Bio-Fibers and Eco-Textiles Qingdao UniversityQingdao266071China Center for Nanoscale Materials Argonne National LaboratoryLemontIL60439USA Center for Advanced Energy Studies Idaho FallsID83401USA

Sodium-ion batteries(SIBs)have stepped into the spotlight as a promising alternative to lithium-ion batteries for large-scale energy storage ***,SIB electrode materials,in general,have inferior performance than their lithium counterparts because Nat is larger and heavier than *** engineering is a promising strategy to overcome this intrinsic limitation and achieve practical *** provide a brief review of recent progress in heterostructure engineering of electrode materials and research on how the phase interface influences Nat storage and transport *** strategies for the design and fabrication of heterostructures(in situ methods)are discussed,with a focus on the heterostructure formation *** heterostructure's influence on Nat storage and transport properties arises primarily from local distortions of the structure and chemomechanical coupling at the phase interface,which may accelerate ion/electron diffusion,create additional active sites,and bolster structural ***,we offer our perspectives on the existing challenges,knowledge gaps,and opportunities for the advancement of heterostructure engineering as a means to develop practical,highperformance sodium-ion batteries.

关键词： Heterostructure Sodium-ion batteries Electrode materials Heterogeneous materials Interface engineering Intergrowth

Fatigue strength and microstructure evaluation of Al 7050 alloy wires recycled by spray forming,extrusion and rotary swaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2020年第12期30卷 3195-3209页

作者： João G.J.de SALVO conrado RMAFONSO Programa de Pós-graduação em Ciência e Engenharia de Materiais(PPG-CEM) Universidade Federal de São Carlos(UFSCar)Rod.Washington LuizKm 23513565-905 São Carlos-SPBrazil Divisão de Materiais Instituto de Aeronáutica e EspaçoCTAPraça Marechal Eduardo Gomes5012228-904 São Josédos Campos-SPBrazil Departamento de Engenharia de Materiais(DEMa) Universidade Federal de São Carlos(USFCar)Rod.Washington LuizKm 23513565-905 São Carlos-SPBrazil

Recycled high-strength aluminum alloys have limited use as structural materials due to poor mechanical properties. Spray forming remelting followed by hot extrusion is a promising route for reprocessing 7 xxx alloys. The 7050 alloy machining chips were spray formed, hot extruded, rotary swaged and heat-treated in order to improve mechanical properties. Microstructures, tensile properties and fatigue strength results for a 2.7 mm-diameter recycled wire are presented. Secondary phases and precipitates were investigated by XRD, SEM, EBSD, TEM and DSC. As-swaged and heat-treated(solution and aging) conditions were evaluated. Mechanical properties of both conditions outperformed AA7050 aerospace specification. Substantial grain refinement resulted from the extensive plastic deformation imposed by rotary swaging. Refined micrometric and sub-micrometric Al grains, as well as coarse and fine intermetallic precipitates were observed. Subsequent solution treatment resulted in a homogeneous, recrystallized and equiaxed microstructure with grain size of 9 μm. Nanoscale GP(I) zones and η′ phase precipitates formed after aging at 120 ℃, imparting higher tensile(586 MPa) and fatigue(198 MPa) strengths.

关键词： Al−Zn−Mg−Cu alloy spray forming thermomechanical processing microstructure fatigue

Buzz-pollination in Neotropical bees:genus-dependent frequencies and lack of optimal frequency for pollen release

在线全文

学校读者我要写书评

暂无评论

Insect Science 2020年第1期27卷 133-142页

作者： conrado Augusto Rosi-Denadai Priscila Cassia Souza Araujo Lucio Antonio de Oliveira Campos Lirio Cosme Jr. Raul Narciso Carvalho Guedes Departamento de Entomologia Universidade Federal de VigosaVigosaMinas GeraisBrazil Departamento de Biologia Geral Universidade Federal de VigosaVigosaMinas GeraisBrazil

Over 50 genera of bees release pollen from flower anthers using thoracic vibrations,a phenomenon known as *** efficiency of this process is directly affected by the mechanical properties of the buzzes,namely the duration,amplitude,and ***,although the effects of the former two properties are well described,the role of buzz frequency on pollen release remains ***,nearly all of the existing studies describing vibrational properties of natural buzz-pollination are limited to bumblebees(Bombus)and carpenter bees(Xvlocopa)constraining our current understanding of this behavior and its ***,we attempted to minimize this shortcoming by testing whether flower anthers exhibit optimal frequency for pollen release and whether bees tune their buzzes to match these(optimal)*** true,certain frequencies will trigger more pollen release and lighter bees will reach buzz frequencies closer to this optimum to compensate their smaller buzz *** strategies were used to test these hypotheses:(i)the use of(artificial)vibrational playbacks in a broad range of buzz frequencies and amplitudes to assess pollen release by tomato plants(Solarium Ivcopersicum L.)and(ii)the recording of natural buzzes of Neotropical bees visiting tomato plants during *** playback experiment indicates that although buzz frequency does affect pollen release,no optimal frequency exists for *** addition,the recorded results of natural buzz-pollination reveal that buzz frequencies vary with bee genera and are not correlated with body ***,neither bees nor plants are tuned to optimal pollen release *** frequency of buzz-pollination is a likely consequence of the insect flight machinery adapted to reach higher accelerations,while flower plant response to buzz-pollination is the likely result of its pollen granular properties.

关键词： buzz amplitude buzz frequency floral sonication pollination stingless bees vibration

Biomimetic mineralized hybrid scaffolds with antimicrobial peptides

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2021年第8期6卷 2250-2260页

作者： Zhou Ye Xiao Zhu Isha Mutreja Sunil Kumar Bod Nicholas G.Fischer Anqi Zhang Christine Lui Yipin Qi conrado Aparicio The State Key Laboratory Breeding Base of Basic Science of Stomatology&Key Laboratory of Oral Biomedicine Ministry of Education School of StomatologyWuhan UniversityWuhan430079China Minnesota Dental Research Center for Biomaterials and Biomechanics Department of Restorative SciencesSchool of DentistryUniversity of MinnesotaMN55455USA Department of Operative Dentistry and Endodontics Guanghua School of StomatologyHospital of StomatologyGuangdong Key Laboratory of StomatologySun Yat-sen UniversityGuangzhou510000China

Infection in hard tissue regeneration is a clinically-relevant *** of scaffolds with dual function for promoting bone/dental tissue growth and preventing bacterial infections is a critical need in the *** we fabricated hybrid scaffolds by intrafibrillar-mineralization of collagen using a biomimetic process and subsequently coating the scaffold with an antimicrobial designer peptide with cationic and amphipathic *** highly hydrophilic mineralized collagen scaffolds provided an ideal substrate to form a dense and stable coating of the antimicrobial *** amount of hydroxyapatite in the mineralized fibers modulated the rheological behavior of the scaffolds with no influence on the amount of recruited peptides and the resulting increase in *** developed scaffolds were potent by contact killing of Gram-negative Escherichia coli and Gram-positive Streptococcus gordonii as well as cytocompatible to human bone marrow-derived mesenchymal stromal *** process of scaffold fabrication is versatile and can be used to control mineral load and/or intrafibrillar-mineralized scaffolds made of other biopolymers.

关键词： Biomimetic mineralization Antimicrobial Cationic and amphipathic peptides Hard tissue cytocompatibility

Development of a 3D matrix for modeling mammalian spinal cord injury in vitro

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第11期11卷 1810-1815页

作者： Juan Felipe Diaz Quiroz Yuping Li conrado Aparicio Karen Echeverri Department of Genetics Cell Biology and Development University of Minnesota Minneapolis MN USA Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute Troy NY USA Minnesota Dental Research Center for Biomaterials and Biomechanics Department of Restorative Sciences School of Dentistry University of Minnesota Minneapolis MN USA

Spinal cord injury affects millions of people around the world, however, limited therapies are available to improve the quality of life of these patients. Spinal cord injury is usually modeled in rats and mice using contusion or complete transection models and this has led to a deeper understanding of the molecular and cellular complexities of the injury. However, it has not to date led to development of successful novel therapies, this is in part due to the complexity of the injury and the difficulty of deciphering the exact roles and interactions of different cells within this complex environment. Here we developed a collagen matrix that can be molded into the 3D tubular shape with a lumen and can hence support cell interactions in a similar architecture to a spinal cord. We show that astrocytes can be successfully grown on this matrix in vitro and when injured, the cells respond as they do in vivo and undergo reactive gliosis, one of the steps that lead to formation of a glial scar, the main barrier to spinal cord regeneration. In the future, this system can be used to quickly assess the effect of drugs on glial scar protein activity or to perform live imaging of labeled cells after exposure to drugs.

关键词： nerve regeneration spinal cord injury 3D scaffold in vitro models astrocytes reactive gliosis neural regeneration

Acute-on-chronic liver failure:Pathogenesis,prognostic factors and management

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第42期21卷 12125-12140页

作者： Sara Blasco-Algora José Masegosa-Ataz María Luisa Gutiérrez-García Sonia Alonso-López conrado M Fernández-Rodríguez Service of Gastroenterology Hospital Universitario Fundación

Acute-on-chronic liver failure(ACLF) is increasingly recognized as a complex syndrome that is reversiblein many cases. It is characterized by an acute deterioration of liver function in the background of a pre-existing chronic liver disease often associated with a high short-term mortality rate. Organ failure(OF) is always associated, and plays a key role in determining the course, and the outcome of the disease. The definition of ACLF remains controversial due to its overall ambiguity, with several disparate criteria among various associations dedicated to the study of liver diseases. Although the precise pathogenesis needs to be clarified, it appears that an altered host response to injury might be a contributing factor caused by immune dysfunction, ultimately leading to a pro-inflammatory status, and eventually to OF. The PIRO concept(Predisposition, Insult, Response and Organ Failure) has been proposed to better approach the underlying mechanisms. It is accepted that ACLF is a different and specific form of liver failure, where a precipitating event is always involved, even though it cannot always be ascertained. According to several studies, infections and active alcoholism often trigger ACLF. Viral hepatitis, gastrointestinal haemorrhage, or drug induced liver injury, which can also provoke the syndrome. This review mainly focuses on the physiopathology and prognostic aspects. We believe these features are essential to further understanding and providing the rationale for improveddisease management strategies.

关键词： Acute on-chronic liver failure Immune dysfunction

声发射和激光拉曼研究镍氧化机制中的稀土元素效应

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

分析测试学报 2004年第3期23卷 22-25,30页

作者：靳惠明 FELIX A conrado 扬州大学机械工程学院材料研究中心江苏扬州225009 Department of Materials Science and Engineering National University of Antioquia

对纯镍及其表面离子注钇样品在1000℃空气中的恒温氧化和循环氧化行为进行了研究。用扫描电镜 (SEM)和透射电镜 (TEM)对氧化膜的表面形貌及结构进行了观测。研究表明离子注钇极大地提高了金属镍的抗氧化性能。此外 ,用声发射 (AE)技术... 详细信息

对纯镍及其表面离子注钇样品在1000℃空气中的恒温氧化和循环氧化行为进行了研究。用扫描电镜 (SEM)和透射电镜 (TEM)对氧化膜的表面形貌及结构进行了观测。研究表明离子注钇极大地提高了金属镍的抗氧化性能。此外 ,用声发射 (AE)技术研究了氧化膜/基体界面上缺陷的分布情况 ,并用激光拉曼(Raman)谱对注钇引起的膜内应力变化进行了测量。结果表明 ,离子注钇降低了NiO氧化膜的生长速率 ,减小了表面NiO的晶粒尺寸 ,降低了膜内压应力水平。同时 ,离子注钇还减小了氧化膜/基体界面缺陷的平均尺寸和数目 ,因此 ,极大地提高了镍表面NiO氧化膜的粘附性和保护性。

关键词：氧化离子注入声发射激光拉曼钇稀土元素效应镍