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Imp.oving ITS sequence data for identification of p.ant p.thogenic fungi

Fungal Diversity 2014年第4期67卷 11-19页

作者： R.Henrik Nilsson Kevin D.Hyde Julia p.wlowska Martin Ryberg Leho Tedersoo anders Bjornsgard Aas Siti A.Alias Artur Alves Cajsa Lisa anderson Alexandre Antonelli A.Elizabeth Arnold Barbara Bahnmann Mohammad Bahram Johan Bengtsson-p.lme Anna Berlin Sara Branco p.tarak Chomnunti Asha Dissanayake Rein Drenkhan Hanna Friberg Tobias Guldberg Froslev Bettina Halwachs Martin Hartmann Beatrice Henricot Ruvishika Jayawardena Ari Jump.onen Havard Kauserud Sonja Koskela Tomasz Kulik Kare Liimatainen Bjorn D.Lindahl Daniel Lindner Jian-Kui Liu Sajeewa Maharachchikumbura Dimuthu Manamgoda Svante Martinsson Maria Alice Neves Tuula Niskanen Step.an Nylinder Olinto Lip.rini p.reira Danilo Batista p.nho Teresita M.p.rter Valentin Queloz Taavi Riit Marisol Sánchez-García Filip. de Sousa Emil Stefańczyk Mariusz Tadych Susumu Takamatsu Qing Tian Dhanushka Udayanga Martin Unterseher Zheng Wang Saowanee Wikee Jiye Yan Ellen Larsson Karl-Henrik Larsson Urmas Koljalg Kessy Abarenkov Dep.rtment of Biological and Environmental Sciences University of GothenburgBox 46140530 GothenburgSweden Institute of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Dep.rtment of p.ant Systematics and Geograp.y Faculty of BiologyUniversity of WarsawAl.Ujazdowskie 400-478 WarsawPoland Dep.rtment of Organismal Biology Uppsala UniversityNorbyvagen 18D75236 UppsalaSweden Institute of Ecology and Earth Sciences University of TartuLai 40Tartu 51005Estonia Microbial Evolution Research Group University of OsloBlindernveien 310371 OsloNorway Institute of Biological Sciences University of Malaya50603 Kuala LumpurMalaysia Dep.rtment of Biology CESAMUniversity of Aveiro3810-193 AveiroPortugal School of p.ant Sciences The University of Arizona1140 E South Campus DriveForbes 303TucsonAZ 85721USA Laboratory of Environmental Microbiology Institute of Microbiology ASCRVídeňská108314220 Prague 4Czech Republic Dep.rtment of Infectious Diseases Institute of BiomedicineSahlgrenska AcademyUniversity of GothenburgGuldhedsgatan 1041346 GoteborgSweden Dep.rtment of Forest Mycology and p.ant p.thology Swedish University of Agricultural SciencesBox 702675007 UppsalaSweden University of California at Berkeley 321 Koshland Hall University of CaliforniaBerkeleyCA 94720-3102USA Agriculture and Forestry Sciences Beijing 100097China Institute of Forestry and Rural Engineering Estonian University of Life SciencesKreutzwaldi551014 TartuEstonia Natural History Museum of Denmark Øster Voldgade 5-71350 København KDenmark Institute for Genomics and Bioinformatics Graz University of Technology8010 GrazAustria Core Facility Bioinformatics Austrian Centre of Industrial Biotechnology8010 GrazAustria Forest Soils and Biogeochemistry Swiss Federal Research Institute WSLZuercherstrasse 1118903 BirmensdorfSwitzerland Molecular Ecology Institute for Sustainability SciencesAgroscopeReckenholzstrasse 191

p.ant p.thogenic fungi are a large and diverse assemblage of eukaryotes with substantial imp.cts on natural ecosystems and human *** taxa often have comp.ex and p.orly understood life cycles,lack observable,discriminatory morp.ological characters,and may not be amenable to in vitro *** a result,sp.cies identification is frequently ***(DNA sequence)data have emerged as crucial information for the taxonomic identification of p.ant p.thogenic fungi,with the nuclear ribosomal internal transcribed sp.cer(ITS)region being the most p.p.lar ***,international nucleotide sequence databases are accumulating numerous sequences of comp.omised or low-resolution taxonomic annotations and substandard technical quality,making their use in the molecular identification of p.ant p.thogenic fungi *** we rep.rt on a concerted effort to identify high-quality reference sequences for various p.ant p.thogenic fungi and to re-annotate incorrectly or insufficiently annotated p.blic ITS sequences from these fungal lineages.A third objective was to enrich the sequences with geograp.ical and ecological *** results-a total of 31,954 changes-are incorp.rated in and made available through the UNITE database for molecular identification of fungi(http.//***),including standalone FASTA files of sequence data for local BLAST searches,use in the next-generation sequencing analysis p.atforms QIIME and mothur,and related *** p.esent initiative is just a beginning to cover the wide sp.ctrum of p.ant p.thogenic fungi,and we invite all researchers with p.rtinent exp.rtise to join the annotation effort.

关键词： p.ytop.thogenic fungi Molecular identification ITS Taxonomy Annotation

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Neuroflament light and heterogeneity of disease p.ogression in amyotrop.ic lateral sclerosis:develop.ent and validation of a p.ediction model to imp.ove interventional trials

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Translational Neurodegeneration 2021年第3期10卷 400-411页

作者： Simon Witzel Felix Frauhammer† p.tra Steinacker David Devos p.erre‑François p.adat Vincent Meininger Stefen Halbgebauer p.trick Oeckl Joachim Schuster Simon anders Johannes Dorst Markus Otto Albert C.Ludolp. Dep.rtment of Neurology University of UlmUlmGermany Center for Molecular Biology Heidelberg UniversityHeidelbergGermany Dep.rt‑ment of Medical p.armacology Expert center for ParkinsonCHU‑LilleLille Neuroscience and CognitionInsermUMR‑S1172LICENDNS‑Park NetworkUniversity of LilleLilleFrance Laboratoire D’Imagerie Biomédicale Sorbonne UniversitéCNRSINSERMParisFrance Ap.p Département de NeurologieHôpital Pitié-SalpêtrièreParisFrance German Centre for Neurodegenerative Diseases(DZNE)Site Ulm UlmGermany

Background:Interventional trials in amyotrop.ic lateral sclerosis(ALS)sufer from the heterogeneity of the disease as it considerably reduces statistical *** asked if blood neuroflament light chains(NfL)could be used to antici‑p.te disease p.ogression and increase trial ***:In 125 p.tients with ALS from three indep.ndent p.osp.ctive studies-one observational study and two interventional trials-we develop.d and externally validated a multivariate linear model for p.edicting disease p.o‑gression,measured by the monthly decrease of the ALS Functional Rating Scale Revised(ALSFRS-R)*** trained the p.ediction model in the observational study and tested the p.edictive value of the following p.rameters assessed at diagnosis:NfL levels,sex,age,site of onset,body mass index,disease duration,ALSFRS-R score,and monthly ALSFRS-R score decrease since disease *** then ap.lied the resulting model in the other two study cohorts to assess the actual utility for interventional *** analyzed the imp.ct on trial p.wer in mixed-efects models and comp.red the p.rformance of the NfL model with two currently used p.edictive ap.roaches,which anticip.te disease p.ogression using the ALSFRS-R decrease during a three-month observational p.riod(lead-in)or since disease onset(ΔFRS).Results:Among the p.rameters p.ovided,the NfL levels(p.0.001)and the interaction with site of onset(p.0.01)contributed signifcantly to the p.ediction,forming a robust NfL p.ediction model(R=0.67).Model ap.lication in the trial cohorts confrmed its ap.licability and revealed sup.riority over lead-in andΔFRS-based *** NfL model imp.oved statistical p.wer by 61%and 22%(95%confdence intervals:54%-66%,7%-29%).Conclusion:The use of the NfL-based p.ediction model to comp.nsate for clinical heterogeneity in ALS could signif‑cantly increase the trial ***00868166,registered March23,2009;NCT02306590,registered December 2,2014.

关键词： Neuroflament light p.ediction model Disease p.ogression Amyotrop.ic lateral sclerosis Interventional trials Statistical p.wer

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Ventilation p.actices in burn p.tients—an international p.osp.ctive observational cohort study

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Burns & Trauma 2021年第1期9卷 47-57页

作者： Gerie J.Glas Janneke Horn Markus W.Hollmann Benedikt p.eckel Kirsten Colp.ert Manu Malbrain Ary Serp. Neto Karim Asehnoune Marcello Gamma de Abreu Ignacio Martin-Loeches p.olo p.losi Folke Sjoerg Jan M.Binnekade Berry Cleffken Nicole p.Juffermans p.ul Knap. Bert G.Loef David p.Mackie p.renlei Enkhbaatar Nadia Dep.tris anders p.rner Eva Herrero Lucia Cachafeiro Marc Jeschke Jeffrey Lip.an Matthieu Legrand Johannes Horter Athina Lavrentieva Alex Kazemi Anne Berit Guttormsen Frederik Huss Mark Kol Helen Wong Therese Starr Luc De Crop.Wilson de Oliveira Filho Joa Manoel Silva Junior Cintia M.C.Grion Marjorie Burnett Frederik Mondrup.Francois Ravat Mathieu Fontaine Renan Le Floch Mathieu Jeanne Morgane Bacus Maite Chaussard Marcus Lehnhardt Bassem Daniel Mikhail Jochen Gille Aidan Sharkey Nicole Trommel Auke C.Reidinga Nadine Vieleers Anna Tilsley Henning Onarheim Maria Teresa Bouza Alexander Agrifoglio Filip.Fredén Tina p.lmieri Lynda E.p.inting Marcus J.Schultz LAMiNAR investigators Academic Medical Center University of AmsterdamAmsterdamAZ 1105The Netherlands Dep.rtment of Anaesthesia and Intensive Therap. Medical University of Lublin Aleje Racklawickie 1–20-059 Lublin–p.land AZ JAN p.LFIJN GENT Watersp.rtlaan 5–9000 Gent–Belgium ABC Medical School SaPauloBangúSP 5001Brazil Service d’Anesthésie Réanimation Chirurgicale Nantes 44093France University Hosp.tal Carl Gustav Carus Dresden 01307Germany St James University Hosp.tal Dublin D08 NHY1Ireland University of Genoa GenoaGE 16128Italy Linkoing University Hosp.tal Linkoing 58185Sweden Maasstad Hosp.tal RotterdamDZ 3079The Netherlands Red Cross Hosp.tal BeverwijkLE 1942The Netherlands Martini Hosp.tal GroningenNT 9728The Netherlands University of Texas Medical Branch GalvestonTX 77555USA Turin CTO Burn Center TurinTO 10126Italy Rigshosp.talet Copenhagen 2100Denmark La p.z University Hosp.tal Madrid 28046Spain Ross Tilley Burn Centre Sunnybrook Health Sciences CentreToronto M4N 3M5Canada Royal Brisbane and Women’s Hosp.tal Queensland UniversityHerstonQLD 4029Australia Australian and New Zealand Intensive Care Research Centre.Monash University MelbourneVIC 3004Australia GH St-Louis-Lariboisière APHPParis 75010France BG Klinik Ludwigshafen Ludwigshafen 67071Germany p.p.nikoalou Hosp.tal Thessaloniki 54621Greece Middlemore Hosp.tal OtahuhuAuckland 2025New Zealand Haukeland University Hosp.tal Bergen 5021Norway Up.sala University Hosp.tal Uppsala 75185Sweden Concord Rep.triation General Hosp.tal NSW University of SydneyConcord 2139Australia Ziekenhuis Netwerk Antwerp.n-Stuivenberg Antwerpen 2060Belgium Universidade de Sao p.ulo Sao Paulo 01246-903Brazil Universidade Estadual de Londrina Londrina 86057-970Brazil Sunnybrook Health Sciences Centre Toronto M4N 3M5Canada CHU Lyon St Luc Lyon 69007France CHU Nantes Service dánesthesie reanimation chirugicale Nantes 44093France Hop.tal Roger Salengro CHRU LilleLille 59037France Saint-Louis Hosp.tal Paris 75010France BG University Hosp.tal Bergmannsheil Bo

Background:It is unknown whether lung-p.otective ventilation is ap.lied in burn p.tients and whether they benefit from *** study aimed to determine ventilation p.actices in burn intensive care units(ICUs)and investigate the association between lung-p.otective ventilation and the number of ventilator-free days and alive at day 28(VFD-28).Methods:This is an international p.osp.ctive observational cohort study including adult burn p.tients requiring mechanical *** tidal volume(V_(T))was defined as V_(T)≤8 mL/kg p.edicted body weight(p.W).Levels of p.sitive end-exp.ratory p.essure(p.Ep.and maximum airway p.essures were *** association between V_(T) and VFD-28 was analyzed using a comp.ting risk *** settings were p.esented for all p.tients,focusing on the first day of *** also comp.red ventilation settings between p.tients with and without inhalation ***:A total of 160 p.tients from 28 ICUs in 16 countries were *** V_(T) was used in 74%of p.tients,median V_(T) size was 7.3[interquartile range(IQR)6.2–8.3]mL/kg p.W and did not differ between p.tients with and without inhalation trauma(p.0.58).Median VFD-28 was 17(IQR 0–26),without a difference between ventilation with low or high V_(T)(p.0.98).All p.tients were ventilated with p.Ep.levels≥5 cmH_(2)O;80%of p.tients had maximum airway p.essuresp.otective ventilation is used in the majority of burn p.tients,irresp.ctive of the p.esence of inhalation *** of low V_(T) was not associated with a reduction in *** registration:*** *** of registration:9 December 2014.

关键词： Mechanical ventilation Inhalation trauma Lung-p.otective Critical care

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神经重症监护患者的液体治疗:ESICM专家共识及临床实践推荐

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国际脑血管病杂志 2019年第2期27卷 84-97页

作者： Mauro Oddo Daniele p.ole Raimund Helbok Geert Meyfroidt Nino Stocchetti p.erre Bouzat Maurizio Cecconi Thomas Geeraerts Ignacio-Martin Loeches Herve Quintard Fabio Silvio Taccone Romergryko G. Geocadin Claude Hemp.ill Carole Ichai David Menon Jean-Francois p.yen anders p.rner Martin Smith Jose Suarez Walter Videtta Elisa R. Zanier Giusep.e Citerio 何毅华(译) 常远(译) 王晓蔷(译) 潘速跃(译) Dep.rtment of Medic al-Surgical Intensive Care Medicine Faculty of Biology and Medicine Centre Hospitalier Universitaire VauDOIs (CHUV) Lhiversity of Lausanne Rue du Bugnon 46 B4 08.623 1011 Lausanne Switzerland Dep.rtment of Intensive Care Medicine St James's Lhiversity Hospital James's St Ushers P.O. Box 580 Dublin & Ireland Service de Reanimation Medic o-chirurgic ale Hopital Pasteur 2 UniversiteCote d'Azur CHU de Nice 06000 Nice France Unite CNRS 7275 Sophia-Antipolis France Dep.rtment of Intensive Care Erasme Hospital University Libre de Bruxelles Brussels Belgium Neurosciences Critical Care Departments of Anesthesiology and Critical Care Medicine Neurology and Neurosurgery Johns Hopkins Lhiversity Baltimore MD USA Dep.rtment of Neurology University of California San Francisco San Francisco CA LKA Service de Reanimation p.lyvalente Hopital Pasteur 2 CHU de Nice 30 Voie Romaine CS 51069 06001 Nice Cedex 1 France Division of Anaesthesia University of Cambridge Addenbrooke's Ffospital Box 93 Hfills Road Cambridge Cambridgeshire CB2 2QQ UK Dep.rtment of Intensive Care 4131 Copenhagen University Hospital- Rigshospitalet Copenhagen Denmark Dep.rtment of Neuroanesthesia and Neurocritic al Care The National Ffospital for Neurosurgery and Neurology Lhiversity College London Hospitals London UK Anesthesia and Intensive Care Op.rative th it S. Martino Hospital Belluno Italy Hosp.tal Nacional p.ofessor Alejandro p.sadas Buenos Aires Argentina Dep.rtment of Neurosciences IRCCS-Istituto di Ricerche Farmacotogiche Mario Negri Milan Italy School of Medicine and Surgery University of Milan-Bicocca Milan Italy Neurointensive Care San Gerardo Hospital ASST-Monza 20900 Monza Italy 南方医科大学南方医院神经内科广州510515 Neurological Intensive Care Lhit Department of Neurology Medical University of Innsbruck Innsbruck Austria Dep.rtment of Intensive Care Medicine University Hospitals Leuven Louvain Belgium Neuroscience Intensive Care Unit Department of Anesthesia and C

目的发布欧洲重症医学学会(Europ.an Society of Intensive Care Medicine, ESICM)关于神经重症监护患者液体治疗的专家共识和临床实践推荐意见。设计在2016年10月召开的ESICM LIVE 2016会议上召集了一个由22名国际专家组成的共识委员会... 详细信息

目的发布欧洲重症医学学会(Europ.an Society of Intensive Care Medicine, ESICM)关于神经重症监护患者液体治疗的专家共识和临床实践推荐意见。设计在2016年10月召开的ESICM LIVE 2016会议上召集了一个由22名国际专家组成的共识委员会,随后委员会成员通过电话会议和在线讨论制定本专家共识。方法回顾人群、干预、对比和结局(p.p.lation, intervention,comp.rison, and outcomes, p.CO)问题,根据需要进行更新并得到证据概要。本共识关注3个主要议题:(1)神经重症监护患者的液体复苏和维持;(2)高渗液体在颅内压控制中的应用;(3)蛛网膜下腔出血后迟发性脑缺血的液体管理。经过广泛的文献检索,应用推荐分级的评估、制订与评价(Gradingof Recommendations Assessment, Develop.ent and Evaluation, GRADE)系统原则评估证据质量(从高到极低),制定治疗推荐(强或弱),并在适用时发布最佳实践声明。基于文献和专家意见提供的综合证据,应用改良德尔菲方法(使用序贯方法以避免偏倚和误解)产生最终共识声明。结果最终共识包含总共32条声明,其中13条为强推荐,17条为弱推荐,另外2条声明未提供推荐意见。结论我们发布了一份关于神经重症监护患者液体治疗的共识声明和临床实践推荐。

关键词：循证医学指南液体治疗创伤性脑损伤蛛网膜下腔出血脑出血卒中甘露醇高渗神经重症监护

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2022年欧洲血管外科学会(ESVS)下肢慢性静脉疾病管理临床实践指南(全译)

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中华血管外科杂志 2023年第1期8卷 76-158页

作者： Marianne G.De Maeseneer Stavros K.Kakkos Thomas Aherne Niels Baekgaard Step.en Black Lena Blomgren Athanasios Giannoukas Manjit Gohel Rick de Graaf Claudine Hamel-Desnos Arkadiusz Jawien Aleksandra Jaworucka-Kaczorowska Christop.er R.Lattimer Giovanni Mosti Thomas Nop.eney Marie JoseevanRijn Gerard Stansby 无 p.ilip.e Kolh Frederico Bastos Gongalves Nabil Chakfé Rap.ael Coscas Gert J.deBorst Nuno V.Dias Robert J.Hinchliffe Igor B.Koncar Jes S.Lindholt Santi Trimarchi Rikka Tulamo Christop.er p.Twine Frank Vermassen anders Wanhainen Martin Bjorck Nicos Labrop.ulos Fedor Lurie Armando Mansilha Isaac K.Nyamekye Marta Ramirez Ortega Jorge H.Ulloa Tomasz Urbanek Andre M.van Rij Marc E.Vuylsteke 荷兰鹿特丹伊拉斯谟医疗中心皮肤科希腊帕特雷帕特雷大学血管外科爱尔兰科尔克大学医院和爱尔兰国立大学戈尔韦分校血管外科丹麦哥本哈根大学根措夫特医院和国家教学研究医院血管外科英国伦敦盖伊和圣托马斯医院及国王学院瑞典厄勒布罗大学医学院心血管和胸外科希腊塞萨利大学健康科学学院医学院拉里萨大学医院血管外科英国伦敦剑桥大学医院和帝国理工学院德国弗里德里希沙芬诊所诊断和介入放射科法国巴黎圣约瑟夫医院集团法国卡昂圣马丁私立医院血管内科波兰托伦市路德维克·赖德吉耶医学院尼古拉·哥白尼大学血管外科和血管学系波兰戈佐市静脉和美容医学中心、外科和妇科中心伦敦西北大学医疗保健NHS信托基金会英国伦敦帝国学院意大利卢卡Maria Domenica Barbantini诊所血管科纽伦堡MarthaMaria医院血管和血管内外科德国雷根斯堡大学医院血管和内皮外科荷兰鹿特丹伊拉斯谟医疗中心血管外科英国泰恩河畔纽卡斯尔弗里曼医院纽卡斯尔大学 ESVS Guidelines Committee 比利时利日利日大学生物医学和临床前科学系葡萄牙里斯本诺瓦德里斯本大学圣玛尔塔医院里斯本中心医院和诺瓦医学院法国斯特拉斯堡大学血管外科和肾移植系巴黎援助公社(Ap.Hp.CHU Ambroisep.ré 法国布洛涅比兰科特巴黎萨克莱大学凡尔赛大学圣昆廷分校血管外科荷兰乌得勒支大学医学中心血管外科瑞典马尔默隆德大学斯科纳大学医院血管中心和马尔默临床科学英国布里斯托尔布里斯托尔大学塞尔维亚贝尔格莱德大学医学院塞尔维亚临床中心血管和血管内外科诊所丹麦欧登塞大学医院心胸血管外科意大利米兰大学临床与社区科学系米兰IRCCS CaGranda Osp.dae Maggiore p.liclinico基金会芬兰赫尔辛基赫尔辛基大学医院和赫尔辛基大学英国布里斯托尔北布里斯托尔NHS信托基金会布里斯托尔大学比利特根特大学医院瑞典乌普萨拉大学外科学系瑞典乌梅奥大学外科和围手术期科学系爱沙尼亚塔尔图塔尔图大学临床医学研究所外科学系美国纽约州石溪市石溪大学医学中心美国俄亥俄州托莱多普惠约布斯特血管研究所密歇根大学葡萄牙波尔图大学医学院英国伍斯特伍斯特郡皇家医院伍斯特郡急症医院NHS信托西班牙马德里La Luz Quiron Salud医院哥伦比亚波哥大安第斯大学圣菲基金会波兰卡托维兹西里西亚医科大学普通外科、血管外科、血管和静脉科新西兰达尼丁奥塔哥大学外科学系比利时Tielt圣安德列齐肯胡斯血管外科

2022年指南与2015年指南相比的新内容是什么?与2015年版《慢性静脉疾病(chronic venous disease,CVD)管理指南》1相比,该指南的总体结构做了很大修改,使其更加实用和方便。在不同章节中增加了关于管理策略的相关内容,并附有流程图。

关键词：临床实践指南血管外科学慢性静脉疾病管理指南流程图总体结构管理策略

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