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The ROCK pathway inhibitor Y-27632 mitigates hypoxia and oxidative stress-induced injury to retinal Müller cells

Neural Regeneration Research 2018年第3期13卷 549-555页

作者： Xiao-hui Zhang zhao-hui feng Xiao-yu Wang Department of Ophthalmology Second Affiliated Hospital of Xi'an Jiaotong University Medical College Xi'an Shaanxi Province China

Rho kinase （ROCK） was the first downstream Rho effector found to mediate RhoA-induced actin cytoskeletal changes through effects on myosin light chain phosphorylation. There is abundant evidence that the ROCK pathway participates in the pathogenesis of retinal endothelial injury and proliferative epiretinal membrane traction. In this study, we investigated the effect of the ROCK pathway inhibitor Y-27632 on retinal Müller cells subjected to hypoxia or oxidative stress. Müller cells were subjected to hypoxia or oxidative stress by exposure to CoCl2 or H2O2. After a 24-hour treatment with Y-27632, the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay was used to assess the survival of Müller cells. Hoechst 33258 was used to detect apoptosis, while 2′,7′-dichlorodihydrofluorescein diacetate was used to measure reactive oxygen species generation. A transwell chamber system was used to examine the migration ability of Müller cells. Western blot assay was used to detect the expression levels of α-smooth muscle actin, glutamine synthetase and vimentin. After treatment with Y-27632, Müller cells subjected to hypoxia or oxidative stress exhibited a morphology similar to control cells. Y-27632 reduced apoptosis, α-smooth muscle actin expression and reactive oxygen species generation under oxidative stress, and it reduced cell migration under hypoxia. Y-27632 also upregulated glutamine synthetase expression under hypoxia but did not impact vimentin expression. These findings suggest that Y-27632 protects Müller cells against cellular injury caused by oxidative stress and hypoxia by inhibiting the ROCK pathway.

关键词： nerve regeneration diabetic retinopathy Rho kinases Müller cells reactive oxygen species glutamine synthetase α-smooth muscle actin CoCl2 H2O2 hypoxia oxidative stress neural regeneration

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pigment epithelium-derived factor protects the morphological structure of retinal Müller cells in diabetic rats

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International Journal of Ophthalmology(English edition) 2014年第6期7卷 941-946页

作者： Xiao-hui Zhang zhao-hui feng Yi Zhang Department of Ophthalmology the Second Affiliated Hospital of Xi'an Jiaotong University Medical College

AIM: To investigate if pigment epithelium-derived factor(PEDF) has any protective effect on the retinal Müller cells of Sprague-Dawley rats suffering from diabetes ***: Sixty Sprague-Dawley rats were randomly divided into a negative control group, a group receiving0.1 μg/μL PEDF, another group receiving 0.2 μg/μL PEDF,and a group receiving balanced salt solution(BSS). Rats in both the PEDF and BSS groups were treated intravitreally based on previously established diabetic models. After 4wk of treatment, morphological alterations of Müller cells and protein expression of glutamine synthase(GS) and glial fibrillary acidic protein(GFAP)were ***:PEDFateither0.1μg/μLor0.2μg/μLsignificantly improved the structures of both nuclei and organelles of Müller cells compared to the BSS-treated *** of GS was significantly higher in the 0.2 μg/μL PEDF group than that in the BSS group(P =0.012), but expression of GFAP was significantly lower in the 0.2 μg/μL PEDF group than that in the BSS group(P =0.000);however, there were no significant differences in expression of these proteins between the 0.1 μg/μL PEDF group and the BSS group(P =0.608, P =0.152). CONCLUSION: PEDF protects the morphological ultrastructure of Müller cells, improves the expression of glutamate synthase and prevents cell gliosis.

关键词： diabetes mellitus pigment epitheliumderived factor retinal Müller cells glutamine synthase glial fibrillary acidic protein

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Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2,3 signal transduction in vitro

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International Journal of Ophthalmology(English edition) 2017年第12期10卷 1805-1811页

作者： zhao-hui feng Xiao-hui Zhang Jia-Qi zhao Jun-Ze Ma Department of Ophthalmology the Second Affiliated Hospital of Xi’an Jiaotong University Medical CollegeXi’an 710004Shaanxi ProvinceChina

AIM：To research the effect of Y-27632,a selective Rhoassociated coiled-coil kinase（ROCK） inhibitor,on TGF-β1/Smad2,3 signal transduction in ocular Tenon＇s capsule fi broblasts（OTFs）.METHODS：Primary ocular Tenon＇s capsule fibroblasts had been cultured in *** effect of Y27632 on proliferation of OTF stimulated by lysophosphatidic acid（LPA） was evaluated by MTT colorimetric assay so as to sift out the proper concentrations range of Y-27632 for the next *** time-polymerase chain reactor（RT-PCR） was to analyze the changes of Smad2 and Smad3 genes of cells affected by Y-27632,though unaffected by transforming growth factorbeta1（TGF-β1）.Proteins of Smad2,Smad3,phosphorylated Smad2（Ser245/250/255）,and phosphorylated Smad3（Ser423/425/203） were respectively quantifi ed by Western blot after OTFs were successively incubated by TGF-β1 and ***,α-smooth muscular actin（α-SMA） protein was also quantified after the small intervening gene fragments of human Smad2 and Smad3 were designed,synthesized,and then transfected to ***：Y-27632 signifi cantly inhibited OTFs proliferation stimulated by *** Y-27632 signifi cantly suppressed the expressions of Smad2 m RNA,Smad2,3 proteins expressions,Smad3 phosphorylation at the carboxylic terminals of Ser423/425/203 which had been radically promoted by TGF-β*** RNA-Smad2,3 suppressed α-SMA expressions,but less effectively than ***：The inhibition of ROCK signaling may be a potential therapeutic candidate for the treatment of the fi ltration channel fi brosis.

关键词： ROCK Y-27632 transforming growth factor-betal Smad human ocular Tenon's capsule fibroblasts

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Association between complementary factor H Y402H polymorphisms and age-related macular degeneration in Chinese: Systematic review and meta-analysis

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International Journal of Ophthalmology(English edition) 2012年第2期5卷 242-246页

作者： Yan-Long Quan Ai-Yi Zhou and zhao-hui feng Department of Ophthalmology the Second Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China

AIM: Age-related macular degeneration (AMD) is the leading cause of blindness in the developed world and complement factor H (CFH) polymorphism has been found to associate with the AMD. To investigate whether the Y402H variant in CFH is associated with AMD in Chinese populations, a systematic review and meta-analysis were performed to estimate the magnitude of the gene effect and the possible mode of action. METHODS: A meta-analysis was performed using data available from ten case-control studies assessing association between the CFH Y402H polymorphism and AMD in Chinese populations involving 1538 AMD. Data extraction and study quality assessment were performed in duplicate. Summary odds ratios (ORs) and 95% confidence intervals (CIs) an allele contrast and genotype contrast were estimated usingfixed- effects models. The Q-statistic test was used to assess heterogeneity, and Funnel plot was used to evaluate publication bias. RESULTS: Seven of ten case-control studies were neovascular AMD, and few studies came from west and north of China. There was strong evidence for association between CFH and AMD in Chinese population, with those having risk allele C 2.35 times more likely to have AMD than subjects with T allele. Evidence of publication bias was not observed in our meta-analysis. CONCLUSION: This meta-analysis summarizes the strong evidence for an association between CFH and AMD in Chinese and indicates each C allele increasing the odds of AMD by 2.33-fold. But more evidences about the relation between CFH polymorphism and different type of Chinese AMD from various district were needed.

关键词： age-related macular degeneration complement factor H polymorphism meta-analysis Chinese population

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Turbulence Modulations in the Boundary Layer of a Horizontal Particle-Laden Channel Flow

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Chinese Physics Letters 2010年第6期27卷 129-132页

作者： LI Jing LIU zhao-hui WANG Han-feng CHEN Sheng LIU Ya-Ming HAN Hai-feng ZHENG Chu-Guang State Key Laboratory of Coal Combustion Huazhong University of Science and Technology Wuhan 430074

Turbulence modulations are experimentally investigated using particle image velocimetry （PIV） in the lower boundary layer of a fully developed horizontal channel flow. A simultaneous two-phase PIV measurement technique is adopted to acquire the turbulent statistics quantities and to examine the coherent structures in the near-wall region. Polythene beads with diameters of 60 μm are used as dispersed phases, and the PIV measurements have been performed at three mass loadings varying from 2.5 ×10^-4 to 5 × 10^-3. All the experiments are performed at a wall shear Reynolds number of Reτ = 430. The results show that the presence of the particles suppresses the coherent structures, with shorter streamwise extent of the quasistrearnwise structures, and then, the wall-normal velocity fluctuations and shear Reynolds stresses are both decreased in the near-core region. In addition, as a result of the particle wake, the turbulence intensity and shear Reynolds stress both increase in the vicinity of the wall. Due to the drag effects of the particles on the gas, the streamwise velocity gradients decrease in the outer region and increase in the viscous sublayer, meanwhile the thickness of the viscous sublayer also decreases. These results cause the peak values of the streamwise velocity fluctuations adjacent to the wall to increase, and the peak positions shift to the wall. This is the reason for decreasing the near-wall region and increasing the near-core region of the streamwise velocity fluctuations in appearance.

关键词： Reynolds stress Wakes Flow in channels Turbulence intensities Statistical analysis Drag

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Comparison of the autofluorescence bronchoscope and the white light bronchoscope in airway examination

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Chinese Journal of Cancer 2010年第12期29卷 1018-1022页

作者： Yun Li Xiao Li Xi-zhao Sui Liang Bu Zu-Li Zhou Fan Yang Yan-Guo Liu hui zhao Jian-feng Li Jun Liu Guan-Chao Jiang Jun Wang Department of Thoracic Surgery People's Hospital Peking University Beijing 100044 P. R. China

Background and Objective: The sensitivity and accuracy of white light bronchoscopy (WLB) in airway examination are low. Autofluorescence bronchoscope (AFB) can determine early lesions in bronchial mucosa more sensitively, but it has seldom performed in China. To assess the clinical value of the AFB in airway examination, we compared the sensitivity and specificity of the AFB and WLB in detecting cancer of the airway mucosa. Methods: Between September 2009 and May 2010, bronchoscope examinations using both the AFB and WLB were performed on 136 patients, 95 men and 41 women with a median age of 61.5 years (ranged from 25 to 84 years). There were 46 lesions located in the central airway, 84 in the peripheral lung parenchyma, and 6 in the mediastinal region. All patients received local and general anesthesia and were subsequently examined with the WLB and AFB in tandem. All procedures were completed safely. Abnormal visual findings were recorded, and biopsies of the affected regions were collected for pathologic examination. Results: Of 241 regions sampled for biopsy, 76 sites contained malignant lesions, whereas 165 sites contained benign lesions. The AFB detected 72 of the 76 malignant lesions, but the WLB detected only 50. The sensitivities of the AFB and WLB were 94.7% and 65.8%, respectively, and the specificities were 57.0% and 83.6%, respectively. The negative predictive values of the AFB and WLB were 95.9% and 84.1%, respectively. Conclusions: The AFB is more sensitive than the WLB in detecting cancerous lesions in the mucosa, and is an effective airway examination.

关键词：病理检查支气管气道萤光自体检测灵敏度空军基地色光

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Demonstration and Radiation Extension mode of Agricultural Technology——A Case of the Science and Technology Project of High Yield in Hebei Province

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Asian Agricultural Research 2010年第2期2卷 37-39,44页

作者： WANG Xiao-ying SHI Zheng YANG Ying-guang zhao hui-feng Agricultural University of Hebei Baoding 071001China

Firstly,relying on the science and technology project of high yield in Hebei Province,connotation of agricultural technology demonstration and extension mode and the high yield grain project in Hebei Province is *** mode of agricultural technology demonstration and radiation is *** technology demonstration and radiation mode includes the radiation center,primary irradiation and secondary ***,management system and operational mechanism of agricultural technology demonstration and radiation mode are discussed,mainly expressed in establishing leading group in each demonstration county(city),carrying out leader contract responsibility system,establishing expert advisor steering group and setting up core experts group for subject,establishing technical experts group for subject,setting up leading group in the radiation area,carrying out chief expert responsibility system and technician matrix responsibility system,establishing the operating mechanism of "open,flow,competition and collaboration" with "test area-expert two-way selection" as the core ***,countermeasures to improve the agricultural technology demonstration and radiation mode is put forward,such as establishing the rural technology demonstration base,strengthening cooperation with enterprises,and adopting flexible technical training,so as to promote the spread of agricultural high-tech,to increase the contribution rate of agricultural technology,and to offer ideas for agricultural technology extension model at the new era.

关键词： Science and technology project of high yield Demonstration and radiation Agricultural extension mode China

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大型齿轮在机测量技术的发展研究

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机械设计与制造 2010年第12期 264-266页

作者：金嘉琦赵丰慧 Jia-qi feng-hui 沈阳工业大学机械工程学院沈阳110870

阐述了国内外大型齿轮在机测量技术及其测量仪器的研究现状,论述了在机测量的优越性及可操作性,提出了大型齿轮在机测量的关键技术难点及解决方法,并进一步分析了大型齿轮在机测量技术的发展趋势。

关键词：大型齿轮测量技术发展研究 research 齿轮在机测量技术的发展趋势研究现状可操作性解决方法技术难点测量仪器优越性国内外分析

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Si/SiO2 Composite Negative Electrode Material for Lithium Ion Batteries

Si/SiO2 Composite Negative Electrode Material for Lithium Io...

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第六届中国储能与动力电池及其关键材料学术研讨与技术交流会

作者： LIU Min ZHANG Na zhao feng-hui zhao Xiao-qin CHEN Ke CHEN Yong Hainan University

As lithium-ion battery anode materials,silicon has the highest specific *** order to restrain pure silicones serious volume change and enhance its electrochemical performance,Si/SiO composites were prepared by using a convenient high energy ball-milling *** characteristics of the composites as anode material for rechargeable lithium-ion batteries were investigated by X-ray diffraction and scanning electron microscopy *** electro-

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近代中国自由主义的类型及演变格局

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烟台大学学报（哲学社会科学版） 2009年第3期22卷 97-105页

作者：俞祖华赵慧峰鲁东大学历史与社会学院山东烟台264025

近代自由主义有不同类型:从学理渊源的角度,可区分为西化自由主义与本土自由主义;从问题领域的角度,可区分为政治自由主义、文化自由主义与经济自由主义;从问政方式的角度,把对"直接参政"感兴趣的行动型自由主义分为认可、容忍、融入现... 详细信息

近代自由主义有不同类型:从学理渊源的角度,可区分为西化自由主义与本土自由主义;从问题领域的角度,可区分为政治自由主义、文化自由主义与经济自由主义;从问政方式的角度,把对"直接参政"感兴趣的行动型自由主义分为认可、容忍、融入现政府的介入型与同样热衷于"直接参政"但与当局互别苗头、另组政党、另走"第三条道路"的组党型,把拒绝"直接参政"的观念型自由主义者分为热衷于舆论干政、办报议政的议政型与"参透"政治因而与政治保持距离的疏离型四类。中国近代自由主义的主体是西化自由主义,西化自由主义内部则有从古典自由主义到新自由主义、社会民主主义的演进过程;但也有思想家相对来说更关注从本土的思想资源中挖掘类似于西方近代自由思想的元素。中国近代自由主义主要发生在政治与文化领域,经济自由主义较为薄弱。从问政方式的角度,以胡适为代表的民间议政型自由主义与民主党派领导人的组党型自由主义,在现代中国政治舞台上发挥了重要的影响。

关键词：近代中国自由主义近代思想史

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