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Fabrication of scaffolds in tissue engineering： A review

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Frontiers of Mechanical Engineering 2018年第1期13卷 107-119页

作者： Peng ZHAO Haibing gu Haoyang MI Chengchen RAO jianzhong FU Lih-sheng TURNG State Key Laboratory of Fluid Power arid Mechatronic Systems Zhejiang University Hangzhou 310027 China Key Laboratory of 3DPrinting Process and Equipment of Zhejiang Province ZhejiangUniversity Hangzhou 310027 China Wisconsin Institute for Discovery University of Wisconsin-MadisonMadison WI 53715 USA

Tissue engineering （TE） is an integrated discipline that involves engineering and natural science in the development of biological materials to replace, repair, and improve the function of diseased or missing tissues. Traditional medical and surgical treatments have been reported to have side effects on patients caused by organ necrosis and tissue loss. However, engineered tissues and organs provide a new way to cure specific diseases. Scaffold fabrication is an important step in the TE process. This paper summarizes and reviews the widely used scaffold fabrication methods, including conventional methods, electrospinning, three-dimensional printing, and a combination of molding techniques. Furthermore, the differences among the properties of tissues, such as pore size and distribution, porosity, structure, and mechanical properties, are elucidated and critically reviewed. Some studies that combine two or more methods are also reviewed. Finally, this paper provides some guidance and suggestions for the future of scaffold fabrication.

关键词： tissue engineering scaffolds electrospinning,3D printing molding techniques conventional methods

Li-MOF-based ions regulator enabling fast-charging and dendrite-free lithium metal anode

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Chinese Chemical Letters 2023年第2期34卷 489-494页

作者： Xiang Han Tiantian Wu Lanhui gu Minfeng Chen jianzhong Song Dan Tian Jizhang Chen College of Materials Science and Engineering Co-Innovation Center of Efficient Processing and Utilization of Forest ResourcesNanjing Forestry UniversityNanjing 210037China

Li metal has been regarded as the holy grail for the next-generation Li-ion *** dendrites issues,however,impede its practical *** general,prolonging the sand time of Li nucleation and regulating homogeneous Li^(+) flux are effective approaches to suppress the dendrites formation and *** this view,a functional polypropylene (PP) separator is developed to regulate ion transportation via a newly designed Li-based metal-organic framework (Li-MOF) *** Li-MOF crystallizes in the orthorhombic space group P212121 and features a double-walled three-dimensional (3D) structure with 1D *** well-defined intrinsic nanochannels of Li-MOF and the steric-hinerance effect both restrict free migration of anions,contributing to a high Li^(+) transference number of 0.65,which improve the Sand time of Li ***,the Li-MOF coating with uniform porous structure promotes homogeneous Li^(+) flux at the surface of Li ***,the Li-MOF coating layer helps to build solid-electrolyte interphase (SEI) layer that comprises of inorganic Li F and Li_(3)N,which further prohibits the dendrites ***,a highly stable Li plating/stripping cycling for over 1000 h is *** functional separator also enables high-performance full lithium metal cells,the high-rate and long-stable cycling performance of Li Ni_(0.8)Mn_(0.1)Co_(0.1)(NMC811)-Li and Li Co O_(2)(LCO)-Li cells further demonstrate the feasibility of this concept.

关键词： Lithium-ion battery Li metal anode Metal-organic framework Li ions flux Solid-electrolyte interphase

雌激素受体-β基因多态性对广西壮族绝经后妇女促卵泡激素、黄体生成素和雌孕激素的影响

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右江民族医学院学报 2018年第6期40卷 529-532页

作者：陈建海李海杨洁梁倩方晓燕黎飚顾建忠右江民族医学院广西百色533000

目的了解雌激素受体(ER)-β基因型对壮族妇女绝经后其血清促卵泡激素、黄体生成素和雌孕激素的影响,为老年女性雌激素缺乏的疾病的提前防治提供个体化方案。方法对379例壮族(广西)45～50岁绝经妇女清晨空腹常规采静脉血5ml,使用PCR-RFL... 详细信息

目的了解雌激素受体(ER)-β基因型对壮族妇女绝经后其血清促卵泡激素、黄体生成素和雌孕激素的影响,为老年女性雌激素缺乏的疾病的提前防治提供个体化方案。方法对379例壮族(广西)45～50岁绝经妇女清晨空腹常规采静脉血5ml,使用PCR-RFLP方法检测并分析ER-β基因分布情况,用放射免疫试剂盒法测定各基因型样本血清的促卵泡激素、黄体生成素、雌二醇和孕激素。结果在对5个ER-β基因RsaI酶切基因(RR、Rr、rr、r、R)组的血清促卵泡激素、黄体生成素和雌二醇、孕酮水平比较中发现:RR基因组、Rr基因组、rr基因组、R基因组,4组间两两比较,差异无统计学意义(P>0.05);而r基因组与其它4组之间比较,差异有统计学意义(P0.05);aa基因组与其它4组之间比较差异有统计学意义(P <0.01),aa基因组的血清雌二醇和孕酮水平明显高于其它4组(P <0.05),而aa基因组的促卵泡激素、黄体生成素明显低于其它4组(P <0.01)。结论携带ER-βRsaI酶切的r等位基因绝经后妇女雌孕激素水平比较低,更易患绝经后雌激素缺乏性老年性疾病,应做好提前防治。而携带ER-βAluI酶切基因的aa等位基因型的绝经后妇女,其雌孕激素水平较高,不易患雌激素缺乏性疾病。

关键词：壮族雌激素受体β 基因多态性绝经后期

Comparison of Cost-effective Distances for LFAC with HVAC and HVDC in Their Connections for Offshore and Remote Onshore Wind Energy

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CSEE Journal of Power and Energy Systems 2021年第5期7卷 954-975页

作者： Xin Xiang Shiyuan Fan Yunjie gu Wenlong Ming jianzhong Wu Wuhua Li Xiangning He Timothy C.Green College of Electrical Engineering Zhejiang UniversityHangzhou 310027China Department of Electronic and Electrical Engineering University of Bath.Bath BA27AYUK. School of Engineering Cardiff UniversityCF243AA CardiffUK. Department of Electrical and Electronic Engineering Imperial College LondonLondonSW72AZUK

For a cost-effective connection of large-scale longdistance wind energy,a low frequency alternating current(LFAC)transmission scheme(16.7 Hz or 20 Hz)is proposed as an alternative to the conventional high voltage alternating current(HVAC)transmission scheme(50 Hz or 60 Hz)and the recently popular high voltage direct current(HVDC)transmission scheme(0 Hz).The technical feasibility of the LFAC system is demonstrated but the basis for identifying the distance ranges for which LFAC would be preferable to HVAC and HVDC are not established and the dependence of this range on factors,such as power transfer rating,voltage rating and cable/line type,is not *** paper presents an in-depth analysis for the overall cost of LFAC system and then provides an extensive comparison with HVAC and HVDC,to explore the distance ranges over which LFAC is cost-effective over both HVAC and HVDC in connections of offshore and remote onshore wind *** results demonstrate that the LFAC system does possess ranges in the intermediate distance for which it is more cost-effective than both HVAC and HVDC,and its overall cost advantage is generally larger in the overhead line(OHL)connection of remote onshore wind energy than the cable connection of offshore wind energy.

关键词： Cost-effective ranges LFAC overall cost analysis wind energy.

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冶金企业通用型联轴节的改进及应用

冶金设备 2006年第3期 72-74页

作者：袁建路程玉璋范玉新谷建中赵金玉严旭升河北工业职业技术学院石家庄050091 邯郸钢铁集团公司邯郸056015

在冶金企业某些特定的条件下,通用型联轴器故障率高、点检润滑不便,无法满足生产要求,邯钢型棒材厂通过技术改进,减少了事故和维护量,延长了寿命,经济效益明显。

关键词：联轴器事故改进

BALB/***^(hr)小鼠的培育及其相关遗传学特性分析

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实验动物与比较医学 2023年第4期43卷 363-370页

作者：谈小倩杨颢唐慧青瞿伟李亮钱珍顾坚忠肖君华徐平上海吉辉实验动物饲养有限公司上海201611 东华大学生物科学与技术研究所上海201620

目的将自发突变培育的SHJHhr小鼠的突变Hr基因导入到遗传背景清晰的BALB/cAShjh近交系小鼠,通过突变基因筛查和遗传学特性分析为后续研究Hr基因突变导致异常表型的分子机制以及该模型的推广应用提供依据。方法采用在表型监测指导下回交... 详细信息

目的将自发突变培育的SHJHhr小鼠的突变Hr基因导入到遗传背景清晰的BALB/cAShjh近交系小鼠,通过突变基因筛查和遗传学特性分析为后续研究Hr基因突变导致异常表型的分子机制以及该模型的推广应用提供依据。方法采用在表型监测指导下回交-互交的育种方法,将自发突变培育的SHJHhr小鼠的突变基因以同源突变导入方式导入到近交系BALB/cAShjh小鼠,经10代后培育成BALB/***^(hr)(简称***^(hr))小鼠。通过多重PCR建库后二代测序的方法,分析***^(hr)小鼠中随机分布于基因组上的90个单核苷酸多态性(single nucleotide polymorphism,SNP)检测位点基因型。然后按照GB/T 14927.1—2008的方法,对***^(hr)小鼠中14个生化位点标记基因进行检测。最后利用全基因组外显子测序技术,检测该小鼠的突变基因。结果自2018年5月至2022年3月,共进行了10代的回交-互交,完成了***^(hr)小鼠品系的构建。在检测的90个SNP位点中,除了rs13484115、rs13484116两个位点不同外,***^(hr)小鼠的其他位点基因型均与受体小鼠BALB/cAShjh相同;生化位点标记基因检测结果显示,***^(hr)小鼠的14个位点全部与受体小鼠相同;全基因组外显子测序发现,该小鼠相比受体小鼠品系存在109个位点突变,包括同义突变71个,终止密码子增加1个,错义突变37个,涉及蛋白质序列改变的基因20个(包括已报告的Hr基因)。结论成功培育了突变导入系***^(hr)小鼠,通过全基因组外显子测序分析发现了3个主要引起表型变异的Hr突变基因及关联突变基因,为该小鼠在生物医学研究中的拓展应用提供了基础数据。

关键词： BALB/cA.Cg.SHJH^(hr)小鼠外显子测序突变基因遗传学特性

高产大果花生新品种开农85的选育及稳产性分析

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山东农业科学 2019年第9期51卷 115-117,120页

作者：郭敏杰芦振华任丽邓丽殷君华苗建利李阳谷建中开封市农林科学研究院

开农85是开封市农林科学研究院利用豫花9331为母本、中花12为父本,采用有性杂交技术及系谱法选择育成的高产稳产大果花生新品种。2016-2017参加河南省夏播花生品种多点联合测试,平均单产5 046.45 kg/hm^2,比对照豫花9327增产4.59%,分别... 详细信息

开农85是开封市农林科学研究院利用豫花9331为母本、中花12为父本,采用有性杂交技术及系谱法选择育成的高产稳产大果花生新品种。2016-2017参加河南省夏播花生品种多点联合测试,平均单产5 046.45 kg/hm^2,比对照豫花9327增产4.59%,分别居参试品种第1、3位;平均百果重218.65 g,平均百仁重87.40 g;中抗花生黑斑病、茎腐病、青枯病、锈病。开农85丰产性、稳产性好,荚果均匀,结实性好,综合性状优良,推广应用前景广阔。2019年通过国家非主要农作物品种登记,适宜河南花生种植区域春播和夏播种植。

关键词：花生开农85 品种选育稳产性

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电子束引发双功能基阳离子交换膜的制备研究

辐射研究与辐射工艺学报 2005年第2期23卷 113-113页

作者：俎建华张剑秋顾建忠郁春辉童龙上海大学射线应用研究所上海201800

A study was made on preparation of cation-exchange membranes including sulfonate and carboxylic acid groups by pre-irradiation grafting of acrylic acid (AA) and sodium styrene sulfonate (SSS) onto high-density polyethylene (HDPE). The grafting yield was strongly dependent on the synthesis conditions such as concentration and pH of the monomer solution, grafting temperature and type of solvent. An easy control over the grafting yield could be achieved by proper selection of reaction conditions. Water absorbance of grafted membranes increases with increasing grafting yield.

关键词：高密度聚乙烯丙烯酸对苯乙烯磺酸钠预辐射接枝 X射线衍射

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早期股骨头缺血坏死的磁共振成像诊断

四川医学 2000年第9期21卷 765-766页

作者：王健忠罗来华顾明马宗黎柯振武胡朝芬徐元昌成都军区总医院 610083

目的　分析早期股骨头缺血坏死 (ANFH)的 MR表现 ,为早期诊断及有效处理提供可靠的影像学依据。方法　回顾性分析 32例 ANFH患者 (4 2个髋关节 )的 MR资料。结果　 T1W 42个股骨头表现为前上区软骨下局灶性均匀或不均匀、边界清晰的呈... 详细信息

目的　分析早期股骨头缺血坏死 (ANFH)的 MR表现 ,为早期诊断及有效处理提供可靠的影像学依据。方法　回顾性分析 32例 ANFH患者 (4 2个髋关节 )的 MR资料。结果　 T1W 42个股骨头表现为前上区软骨下局灶性均匀或不均匀、边界清晰的呈楔形、节段形、带状或环状 T1W 低信号 38号 ,等信号 2例 ,混杂信号 2例 ;T2 W 高信号 32例 ,低信号 1例 ,等信号 7例 ,混杂信号 2例。 STIR序列上均为高信号 ,且范围大于 T2 W 。关节腔积液 17例 (4 0 .5 % ) ,“裂隙征”2 5例 (5 9.5 % )。结论　 1广泛均匀的水肿是 ANFH极早期表现。 T1W1呈楔形、节段型、带状或环状低信号为ANFH最常见 MRI表现。 2“裂隙征”是 ANFH的一个重要 MRI征象。 3关节腔积液在 ANFH的诊断和鉴别诊断上有重要意义。 4早期所见极易与一过性骨质疏松症混淆 ,随访观察对鉴别有重要价值。

关键词：早期股骨头缺血坏死磁共振成像诊断