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Effect of Sirpα1 on the expression of nuclear factor-kappa B in hepatocellular carcinoma

hepatobiliary & Pancreatic Diseases International 2007年第3期6卷 276-283页

作者： Jian-Min Qin,Xing-Wang Wan,Jin-Zhang Zeng and Meng-Chao Wu Department of hepatobiliary and Pancreatic surgery,Beijing Chaoyang hospital,Capital university of medical Sciences,Beijing 100020,china and eastern hepatobiliary surgery hospital,second military medical university,shanghai 200438, china Department of hepatobiliary and Pancreatic surgery Beijing Chaoyang Hospital Capital University of Medical Sciences Beijing 100020 China. jianminqin@***

BACKGROUND:Signal regulatory protein alpha1(Sirpα1) is a member of Sirps families containing four immunoreceptor tyrosine-based inhibitory motifs(ITIMs) domains in the cytoplasm of and an activated substrate of receptor tyrosine kinase(RTK),that negatively regulates the RTK-dependent cell proliferating signal transduction *** we found that Sirpα1 was closely associated with the occurrence and development of hepatocellular carcinoma(HCC)as well as liver *** it is unclear about the regulatory mechanisms,we established the cell line transfected Sirpα1 gene and preliminarily clarified the mechanisms by which Sirpα1 negatively regulates the carcinogenesis and development of HCC. METHODS:Liver cancer Sk-Hep1 cell was respectively transfected with plasmids of pLXSN,pLXSN-Sirpα1 and pLXSN-Sirpα1Δ4Y 2 ,screened with the drug of G418(1200 μg/ml),and various transfected Sk-Hep1 cell lines were *** protein expressions of P65,P50,IκBα,cyclin D1 and Fas in various Sk-Hep1 cell lines were determined by Western blotting,and P65 and P50 were localized by the immunofluorescence technique. RESULTS:Sirpα1 could significantly upregulate the protein expression of IκBα(*** cell lines,P<0.05) in the Sk-Hep1 cell,and downregulate the protein expressions of P65,P50 and cyclin D1(*** cell lines, P<0.05)in the Sk-Hep1 cell.P65 protein expression was mainly localized in the cytoplasm in the pLXSN Sk-Hep1 cell,and in the nucleus of the Sk-Hep1 cell with mutantSirpα1Δ4Y 2 ,but in nucleus of the Sk-Hep1 cell with wild Sirpα1.P50 protein expression was localized in the cytoplasm and nucleus of the pLXSN Sk-Hep1 cell,but in the nucleus of the Sk-Hep1 cell with wild Sirpα1 and mutant Sirpα1Δ4Y 2 plasmid. CONCLUSIONS:Sirpα1 might negatively regulate and control the abnormal proliferation of liver cancer cells by influencing the protein content and localization of nuclear factor-kappa B,then influence the expression of cyclins such as cyclin D1 in the si

关键词： signal regulatory protein alpha1 carcinoma,hepatocellular nuclear transcription factor

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Percutaneous cryoablation after chemoembolization of liver carcinoma: report of 34 cases

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hepatobiliary & Pancreatic Diseases International 2003年第4期2卷 520-524页

作者： Guo-Jun Qian Han Chen Meng-Chao Wu the eastern hepatobiliary surgery hospital, second military medical university, shanghai 200438, china eastern hepatobiliary surgery hospital Second Military Medical University Shanghai 200438 China. qgjs@***

OBJECTIVE: To assess the indications, efficacy and clinical significance of percutaneous cryoablation for liver carcinoma after transcatheter liver artery chemoembolization (TACE). METHODS: Thirty-four patients with histologically or clinically confirmed primary or metastatic carcinomas were treated with TACE. One week to 1 month later, they were treated percutaneously under ultrasound guidance using cryosurgical system in the period of July 2001 -June 2002. All patients were followed up to determine serum tumor marker, CT scans, MRI images or ultrasound images. RESULTS: This therapy was performed in 34 patients including 32 patients with Child A liver reserve, 2 patients with Child B and no patient with Child C. There were 28 patients with primary liver cancer and 6 patients with metastatic liver cancer. During the follow-up period (3 to 15 months), 41.1% patients were recognized clinically cured because the serum tumor markers became normal, or CT scans and MRI images revealed that the lesion became completely necrotic. 44.1% patients were recognized effectively treated. CONCLUSIONS: Percutaneous cryoablation combined with TACE is a choice of treatment for liver carcinoma. It is minimally invasive, safe and effective for those patients with liver cancer unsuitable for surgery.

关键词： liver neoplasm cryosurgery chemoembolization

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Combined invagination and duct-to-mucosa techniques with modifications:anew method of pancreaticojejunal anastomosis

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hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 422-427页

作者： Bin Zhu,Li Geng,You-Gang Ma,Yong-Jie Zhang and Meng-Chao Wu second Department of Biliary surgery and Department of Special Treatment,eastern hepatobiliary surgery hospital,second military medical university,shanghai 200438,china [a] second Department of Biliary surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China [b] Department of Special Treatment Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND:Soft pancreatic texture and a small main pancreatic duct are thought to be the most significant risk factors for the occurrence of pancreatic fistula (PF),a common and serious complication after pancreaticoduodenectomy (PD).This is in part due to the technical difficulties of pancreaticojejunostomy (PJ) posed by a soft gland with a normal-sized *** deal with this problem,we developed a new anastomotic technique which combines the two most widely used techniques,namely,the invagination technique and the duct-to-mucosa technique,with a modification of the suture route and insertion of a temporary stent ***:Between January 2003 and December 2009,ninetytwo consecutive patients underwent PD in which the new PJ technique was *** and follow-up data of these patients were reviewed for operative details,early postoperative events,and outcomes at 6 months after the *** was defined by the International Study Group on Pancreatic Fistula (ISGPF) guidelines and graded (A,B or C) according to the clinical procedures and ***:In this group of 92 patients,there was only 1 early death from acute renal *** was observed in 11 patients (12.0%),8 in grade A,1 in grade B,and 2 in grade *** the 2 patients in grade C,PF was surgically *** were no early or late deaths attributable to *** months after the operation,all of the patients were free of PJ-related symptoms except for 2,who were found to have ***:Our modified technique is simple and safe in *** data suggest that this technique produces excellent early and medium-term results.

关键词： pancreaticoduodenectomy pancreaticojejunostomy pancreatic fistula

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Influence of preoperative transcatheter arterial chemoembolization on liver resection in patients with resectable hepatocellular carcinoma

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hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 523-526页

作者： Yun-Quan Luo Yi Wang Han Chen Meng-Chao Wu the eastern hepatobiliary surgery hospital, second military medical university, shanghai 200438, china eastern hepatobiliary surgery hospital Second Military Medical University Shanghai 200438 China. yqluo@***

Objective: To assess the influence of preoperative transcatheter arterial chemoembolization (TACE) on liver resection in patients with resectable hepato- cellular carcinoma (HCC). Methods: Of 126 patients with resectable HCC, 62 received preoperative TACE (TACE group) and the remaining 64 patients were selected as controls (non- TACE group). Perioperative risk factors including liver function alteration, mean blood loss during op- eration, mean time of clamping the porta hepatis, length of operation, postoperative abdominal drain- age at day 1, 2 and 3, morbidity and mortality were compared between the two groups. Results: Neither significant difference in liver func- tion alteration nor mortality was observed between the two groups. More severe hepatic cirrhosis, lon- ger operation time, more blood loss and postopera- tive abdominal drainage were noted in the TACE group than in the non-TACE group. There was no significant difference in postoperative morbidity be- tween the two groups. Conclusions: Preoperative TACE for resectable HCC increases surgical difficulty and risk, and therefore should be considered prudently according to the indi- viduality of patients.

关键词： hepatocellular carcinoma therapeutic chemoembolization hepatectomy

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Prognostic factors in the surgical treatment of caudate lobe hepatocellular carcinoma

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World Journal of Gastroenterology 2010年第9期16卷 1123-1128页

作者： Peng Liu,Jia-Mei Yang,Wen-Yang Niu,Tong Kan,Feng Xie,Dian-Qi Li,Ye Wang,Yan-Ming Zhou,Department of Special Treatment and Liver Transplantation,eastern hepatobiliary surgery hospital,second military medical university,shanghai 200438,china Peng Liu,Department of hepatobiliary surgery,Navy General hospital,Beijing 100037,china Department of Special Treatment and Liver Transplantation Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China. jmyang@***

AIM:To evaluate the short-and long-term outcomes of liver resection for caudate lobe hepatocellular carcinoma (HCC).METHODS:We retrospectively analyzed 114 consecutive patients with HCC,originating from the caudate lobe,who underwent resection between January 2001 and January *** and multivariate analyses were performed on several clinicopathologic variables to determine the factors affecting long-term outcome and intrahepatic ***:Overall mortality and morbidity were 0% and 18%,*** a median follow-up of 31 mo (interquartile range,11-66 mo),tumor recurrence had occurred in 76 patients (66.7%).The 1-,3-,and 5-year disease-free survival rates were 65.7%,38.1%,and 18.4%,*** 1-,3-,and 5-year overall survival rates were 76.1%,54.7%,and 31.8%,*** analysis showed that subsegmental location of the tumor (45.7% vs 16.2%,P=0.01),liver cirrhosis (12.3% vs 47.9%,P=0.03),surgical margin (18.5% vs 54.6%,P=0.04),vascular invasion (37.9% vs 23.2%,P=0.04) and extended caudate resection (42.1% vs 15.4%,P=0.04) were related to poorer long-term *** analysis showed that only subsegmental location of the tumor,liver cirrhosis and surgical margin were significant independent prognostic ***:Hepatectomy was an effective treatment for HCC in the caudate *** subsegmental location of the tumor,liver cirrhosis and surgical margin affected long-term survival.

关键词： Hepatectomy Hepatocellular carcinoma Caudate lobe Prognostic factors

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Expression of HBx protein in hepatitis B virus-infected intrahepatic cholangiocarcinoma

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hepatobiliary & Pancreatic Diseases International 2012年第5期11卷 532-535页

作者： Yan-Ming Zhou, Lu Cao, Bin Li, Xiu-Zhong Zhang, Zheng-Feng Yin Department of Hepato-Biliary-Pancreato-Vascular surgery, First Affiliated hospital of Xiamen university, Xiamen 361003, china Department of Molecular Oncology,Department of Pathology, eastern hepatobiliary surgery hospital,second military medical university, shanghai 200438, china [a] Department of Hepato-Biliary-Pancreato-Vascular surgery First Affiliated Hospital of Xiamen University Xiamen 361003 Shanghai China [b] Department of Molecular Oncology Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 Shanghai China [c] Department of Pathology Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 Shanghai China

BACKGROUND: Hepatitis B virus (HBV) is an etiological factor of intrahepatic cholangiocarcinoma (ICC), but the pathogenic mechanisms remain unclear. This study aimed to investigate the expression and possible role of HBx, an HBV- encoded potentially oncogenic protein, in HBV-infected ICC. METHODS: Tissue samples were obtained from 54 specimens of HBV-infected ICC. Forty-four specimens were of peripheral type and 10 hilar type. Formalin-fixed, paraffin-embedded sections of the specimens were immunohistochemically stained for HBx and p53. RESULTS: HBx expression was found in 70.4% (38/54) of the specimens, and it was more frequently seen in the peripheral type than in the hilar type (79.5% vs 30.0%, P=0.002). All three well-differentiated ICCs expressed HBx, whereas 76.9% (30/39) moderately-differentiated and 41.7% (5/12) poorly-differentiated ICCs had HBx expression (P=0.033). Patients with HBx expression had a significantly higher prevalence of elevated serum alpha-fetoprotein (P=0.033). p53 protein expression was found in 18 of 54 cases (33.3%), and was not correlated with that of HBx. CONCLUSIONS: HBx may contribute to the pathogenesis of ICC, particularly the peripheral type. p53 abnormality may not play a significant role in HBx-mediated oncogenicity during ICC carcinogenesis.

关键词： hepatitis B virus HBx protein p53 intrahepatic cholangiocarcinoma

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E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma

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hepatobiliary & Pancreatic Diseases International 2002年第1期1卷 80-82页

作者： Bao-Hua Zhang Han Chen Xiao-Ping Yao Wen-Ming Cong Meng-Chao Wu From the eastern hepatobiliary surgery hospital, second military medical university of PLA, shanghai 200438, china eastern hepatobiliary surgery hospital Second Military Medical University of PLA Shanghai 200438 China. zhbh_1@***

Objective: To study the significance of E-selectin andits ligand-sLeX in the metastasis of hepatocellularcarcinoma (HCC).Methods: Flow cytometry and immunohistochemistrywere used to detect the expression of E-selectin and itsligand-sLeX in both HCC cell lines and human ***: The positive rate of E-selectin in vascular en-dothelial cells adjacent to cancer was 67.9% (19/28). Theexpression of E-selectin in tumors accompanied withemboli or satellite foci was significantly higher than thatin tumors without emboli or satellite foci (P<0.05),and it was not related to tumor size, tumor capsule,AFP content, and the degree of differentiation. Thepositive expression of sLeX in SMMU-7721, PLF/PRF/5 and HepGⅡ cell lines was 7.03%, 63.35% and97.29% respectively. The positive cells of sLeX mainlydistributed in the margin of tumor tissues. The positiveexpression of sLeX in HCC cells in emboli or invasivetumor tissues was much higher than in primary ***: E-selectin and its ligand-sLeX are closelycorrelated with the metastasis of HCC.

关键词： liver neoplasm E-selectin sLeX metastasis

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Changes of serum alpha-fetoprotein and alpha-fetoprotein-L3 after hepatectomy for hepatocellular carcinoma: prognostic significance

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hepatobiliary & Pancreatic Diseases International 2012年第6期11卷 618-623页

作者： Xiao-Feng Zhang, Zheng-Feng Yin, Kui Wang, Zong-Qin Zhang, Hai-Hua Qian,Le-Hua ShiAuthor Affiliations: The 4th Department of Hepatic surgery ,Molecular Oncology Laboratory , eastern hepatobiliary surgery hospital, second military medical university, shanghai 200438, china [a] The 4th Department of Hepatic surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China [b] Molecular Oncology Laboratory Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND: Alpha-fetoprotein (AFP) is the most established tumor marker of hepatocellular carcinoma (HCC), but one of its limitations is non-specificity. Many studies have demonstrated that alpha-fetoprotein-L3 (AFP-L3) is more specific than AFP in the early diagnosis and prognosis of HCC. However, there is a lack of knowledge about the post-hepatectomy profiles of serum AFP and AFP-L3 values in HCC patients. To identify the profiles after surgical resection of HCC, we analyzed the correlation between the profiles and postoperative HCC recurrence or survival, and evaluated their utility in predicting postoperative therapeutic efficacy and prognosis. METHODS: From August 2003 to December 2004, 318 patients with positive serum AFP who had received surgical resections were enrolled in this study. Preoperative and postoperative serum AFP and AFP-L3 levels were measured simultaneously and regularly, and their postoperative profiles during a long term follow-up were recorded and summarized. RESULTS: A high ratio of AFP-L3 to total AFP was shown to correlate with pathologic features of aggressiveness. The overall 1-, 3-, and 5-year recurrence rates of the whole series were 28% 57%, and 84%, and the overall survival rates were 86%, 61% and 33%, respectively. The changes of serum AFP and AFP-L3 after hepatectomy for HCC were classified into 3 groups (group A: AFP-L3 undetectable; group B: AFP-L3 10%). Patients with positive postoperative AFP-L3had significantly earlier recurrence than those with negative results. The overall survival was significantly shorter in the positive groups than in the groups negative for postoperative ***: Post-hepatectomy changes in serum AFP and AFP-L3 levels occurred in three distinct patterns, which were closely correlated with HCC recurrence and patient survival with different prognostic values.

关键词： hepatocellular carcinoma hepatectomy alpha-fetoprotein alpha-fetoprotein-L3

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Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis

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World Journal of Gastroenterology 2012年第4期18卷 349-355页

作者： Qian Zhang,Department of Infectious Diseases,Changhai hospital,second military medical university,shanghai 200433,china Zhi-Qing Li,Hu Liu,Jia-He Yang,Department of Hepatic surgery,eastern hepatobiliary surgery hospital,second military medical university,shanghai 200438,china Qian Zhang Department of Infectious Diseases Changhai Hospital Second Military Medical University Shanghai 200433 China Zhi-Qing Li Hu Liu Jia-He Yang Department of Hepatic Surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

AIM:To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 antibody (preS2Ab) against HBV in-fection and HBV-associated hepatic ***:An adenoviral vector carrying the full-length light and heavy chains of the HBV preS2Ab gene,Ad315-preS2Ab,was *** linked immunosorbent assay (ELISA) and Western blotting analyses were used to determine the preS2Ab expres-sion levels in *** techniques were used to examine the binding affinity between the expressed HBV preS2Ab and HBV-positive liver *** were also used to determine hepatitis B surface antigen (HBsAg) levels to assess the inhibitory effect of the preS2Ab against HBV infection in L02 *** inhibitory effect of preS2Ab against hepatic carcinogen-esis was studied with diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs) in HBV transgenic ***:The expression of HBV preS2Ab increased with increases in the multiplicity of infection (MOI) of Ad315-preS2Ab in L02 cells,with 350.87 ± 17.37 μg/L of preS2Ab when the MOI was 100 plaque forming units (pfu)/*** expressed preS2Abs could recog-nize liver cells from HBV transgenic *** results showed that L02 cells expressing preS2Ab produced less HBsAg after treatment with the serum of HBV pa-tients than parental L02 cells expressing no *** transgenic mice treated with Ad315-preS2Ab had fewer and smaller cancerous nodes after induction with DEN than mice treated with a blank Ad315 vec-tor or untreated ***,the administration of Ad315-preS2Ab could alleviate hepatic cirrhosis and decrease the serum levels of alanine transaminase and aspartate ***:Adenovirus-mediated HBV preS2Ab expression could inhibit HBV infection in L02 cells,and then inhibit DEN-induced hepatocellular carcinogenesis and protect hepatic function in HBV transgenic mice.

关键词： Hepatitis B virus Adenoviral vector PreS2 antibody Hepatocellular carcinoma Gene therapy

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Risk factors of intrahepatic cholangiocarcinoma in patients with hepatolithiasis:a case-control study

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hepatobiliary & Pancreatic Diseases International 2011年第6期10卷 626-631页

作者： Zhen-Yu Liu,Yan-Ming Zhou,Le-Hua Shi and Zheng-Feng YinAuthor Affiliations:Molecular Oncology Laboratory and Department of Comprehensive Treatment I,eastern hepatobiliary surgery hospital,second military medical university,shanghai 200438,china Department of Hepato-Biliary-Pancreato-Vascular surgery,First Affiliated hospital,Xiamen university,Xiamen 361003,china Molecular Oncology Laboratory Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Department of Comprehensive Treatment I Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Department of Hepato-Biliary-Pancreato-Vascular surgery First Affliated Hospital Xiamen University Xiamen 361003 China

BACKGROUND:Why 3.3% to 10% of all patients with hepatolithiasis develop intrahepatic cholangiocarcinoma (ICC) remains *** carried out a hospital-based case-control study to identify risk factors for the development of ICC in patients with hepatolithiasis in ***:Eighty-seven patients with pathologically diagnosed hepatolithiasis associated with ICC and 228 with hepatolithiasis alone matched by sex,age (±2 years),hospital admittance and place of residence were interviewed during the period of *** ratios (OR) and 95% confidence intervals (CI) were calculated for each risk ***:Among the patients with hepatolithiasis associated with ICC,the mean age was 57.7 years and 61.0% were female Univariate analysis showed that the significant risk factors for ICC development in hepatolithiasis were smoking,family history of cancer,appendectomy during childhood (under age 20),and duration of symptoms >10 *** multivariate stepwise logistic regression analysis,smoking (OR=1.931,95% CI:1.000-3.731),family history of cancer (OR=5.175,95% CI:1.216-22.022),and duration of symptoms >10 years (OR=2.348 95% CI:1.394-3.952) were independent ***:Smoking,family history of cancer and duration of symptoms >10 years may be risk factors for ICC in patients with hepatolithiasis.

关键词： risk factors intrahepatic cholangiocarcinoma hepatolithiasis case-control study

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