检索结果-南通市图书馆

Journal of Animal Science and Biotechnology 2023年第6期14卷 2483-2495页

作者： yingyu Mu Wangpan Qi Tao Zhang Jiyou Zhang Shengyong Mao Ruminant Nutrition and Feed engineering Technology Research Center College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthNational Center for International Research on Animal Gut NutritionCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Background Bovine milk is an important source of nutrition for human consumption,and its quality is closely associated with the microbiota and metabolites in *** there is limited knowledge about the milk microbiome and metabolome in cows with subacute ruminal *** Eight ruminally cannulated Holstein cows in mid lactation were selected for a 3-week *** cows were randomly allocated into 2 groups,fed either a conventional diet(CON;40%concentrate;dry matter basis)or a high-concentrate diet(HC;60%concentrate;dry matter basis).Results The results showed that there was a decreased milk fat percentage in the HC group compared to the CON *** amplicon sequencing results indicated that the alpha diversity indices were not affected by the HC *** the phylum level,the milk bacteria were dominated by Proteobacteria,Actinobacteria,Bacteroidetes,and Firmicutes both in the CON and HC *** the genus level,the HC cows displayed an improved proportion of Labrys(P=0.015)compared with the CON *** of both the principal components analysis and partial least squares of discriminant analysis of milk metabolome revealed that samples of the CON and HC groups clustered separately.A total of 31 differential metabolites were identified between the two *** these,the levels of 11 metabolites decreased(α-linolenic acid,prostaglandin E2,L-lactic acid,L-malic acid,3-hydroxysebacic acid,succinyladenosine,guanosine,pyridoxal,L-glutamic acid,hippuric acid,and trigonelline),whereas the levels of the other 20 metabolites increased in the HC group with respect to the CON group(P<0.05).Conclusion These results suggested that subacute ruminal acidosis less impacted the diversity and composition of milk microbiota,but altered the milk metabolic profiles,which led to the decline of the milk quality.

关键词： High-concentrate diet Milk bacteria Milk metabolome Milk quality Subacute ruminal acidosis

Genetic diversity,population structure and core collection analysis of Hunan tea plant germplasm through genotyping-by-sequencing

在线全文

学校读者我要写书评

暂无评论

Beverage Plant Research 2022年第1期2卷 36-42页

作者： Feiyi Huang Jihua Duan Yu Lei Zhen Liu Yankai Kang Yi Luo yingyu Chen Youyong Li Shuoqian Liu Saijun Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China Tea Research Institute Hunan Academy of Agricultural Sciences/National Centre for Tea ImprovementHunan Branch/Hunan Tea Variety and Seedling Engineering Technology Research CenterChangsha 410125China Tea Research Institute Yunnan Academy of Agricultural SciencesMenghai 666200China

Tea is considered to be a well-known and widely consumed beverage and Hunan province is rich in tea plant *** order to better conserve and utilize Hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of Hunan tea plant germplasm through genotyping by sequencing(GBS)*** a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were *** structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three *** genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to *** of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in Hunan tea ***,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire *** work should be valuable for conservation and utilization of tea germplasm in Hunan。

关键词： Hunan germplasm analysis

Risk factors associated with Salmonella in dairy cattle farms in Henan and Hubei provinces,China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Diseases 2023年第4期3卷 256-264页

作者： Jie Wang Xiaojie Zhu Yuxi Zhao Yongkang Xue Zhen Zhang Lei Yan yingyu Chen Ian D.Robertson Aizhen Guo Joshua W.Aleri The State Key Laboratory of agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China School of Veterinary Medicine College of ScienceHealthEngineering and EducationMurdoch University90 South StreetMurdochWA 6150Australia Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology Huazhong Agricultural UniversityWuhan 430070China Henan Dairy Herd Improvement Center Zhengzhou 450046China Centre for Animal Production and Health Future Foods InstituteMurdoch University90 South StreetMurdochWA 6150Australia

A cross-sectional study was conducted to evaluate the risk factors associated with the Salmonella infection status of dairy herds in Henan and Hubei provinces,*** were assigned a Salmonella status based on the isolation of Salmonella from fecal samples obtained from adult lactating dairy *** on potential biosecurity risk factors was collected using a questionnaire template via a 15-min face-to-face interview with dairy cattle producers from May 2020 to March *** questionnaire consisted of 23 questions across two broad categories of potential biosecurity risk factors:farm and biosecurity managemental factors.A total of 48 farms were *** all(100%,48/48)of the surveyed farms,although sick animals were separated from healthy animals using fencing,there were no strict quarantine protocols for newly introduced *** species rearing was practiced in 35.4%(17/48)of the *** were removed more than once a day in 45.8%(22/48)of the farms,whereas the remainder(54.2%,26/48)only removed animal feces once a day.A total of 29.2%(14/48)of the farms were located<2 km from other livestock *** use of personal protective equipment was consistently performed on all farms,with a major-ity of the workers on most farms(81.3%,39/48)always disinfecting footwear before entering production areas.A significant association between Salmonella-positive status and the high frequency of the presence of wildlife(birds and rodents in sheds and feed storage places)was recorded(OR:11.9,95%Cl:1.7,84.1,p=0.013).Fortunately,no farms shared farm equipment with other *** study highlights the occurrence of wildlife as a risk factor for the presence of Salmonella in investigated dairy *** is a need to institute appropriate on-farm control measures for wild birds and rodents to control the potential spread of Salmonella in dairy production systems.

关键词： Cattle Management practices Risk factors Salmonella

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

SHR调控植物根系发育研究进展

植物生理学报 2021年第2期57卷 337-346页

作者：姜颖茹李祖亮董寰河南农业大学生命科学学院郑州450002 中国科学院植物研究所植物分子生理学重点实验室北京100093 河南大学生命科学学院棉花生物学国家重点实验室植物逆境生物学重点实验室河南开封475004

根系作为植物体重要器官之一,具有高度发育可塑性,植物体可以通过根形态和生理改变对环境胁迫或外源植物生长调节剂作出响应。转录因子SHORT-ROOT(SHR)在根的辐射模式和根顶端分生组织的形成过程中发挥重要作用。本文从SHR参与的信号转... 详细信息

根系作为植物体重要器官之一,具有高度发育可塑性,植物体可以通过根形态和生理改变对环境胁迫或外源植物生长调节剂作出响应。转录因子SHORT-ROOT(SHR)在根的辐射模式和根顶端分生组织的形成过程中发挥重要作用。本文从SHR参与的信号转导通路以及SHR与植物激素共同调控根系发育过程等方面进行综述,有助于理解植物细胞发育和命运决定这一重大理论问题,并将为运用基因工程技术改善作物的根系构型,增强植物环境适应能力提供理论基础。

关键词： SHORT-ROOT 信号转导根系发育环境胁迫

西葫芦CpMYB-TRF1基因克隆和表达模式分析

在线全文

学校读者我要写书评

暂无评论

植物生理学报 2022年第2期58卷 281-293页

作者：张欣陈军宏薛应钰甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室兰州730070

西葫芦(Cucurbita pepo)种皮发育过程中,厚壁组织解体造成种皮消失,形成西葫芦裸仁(hull-less)品种。为了对西葫芦裸仁性状成因和新品种培育提供理论依据,本研究在前期转录组测序获得的西葫芦裸仁品种和有壳品种差异基因的基础上,对CpMY... 详细信息

西葫芦(Cucurbita pepo)种皮发育过程中,厚壁组织解体造成种皮消失,形成西葫芦裸仁(hull-less)品种。为了对西葫芦裸仁性状成因和新品种培育提供理论依据,本研究在前期转录组测序获得的西葫芦裸仁品种和有壳品种差异基因的基础上,对CpMYB-TRF1进行了克隆以及生物信息学、时序表达和组织特异性表达分析。实验结果表明:CpMYB-TRF1编码含608个氨基酸的多肽;系统进化分析显示CpMYB-TRF1蛋白具有较强的种属特异性,与同属的笋瓜(***)、同科的苦瓜(Momordica charantia)亲缘关系最近;时序表达分析显示CpMYB-TRF1在两个品种的西葫芦中表达量变化趋势一致,均在授粉后18 d达到表达量峰值,之后下调表达,且裸仁品种的表达量均高于有壳品种;组织特异性表达分析显示该基因有很强的组织特异性,在不同部位的表达量有很大差异,并且该基因在不同品种间的表达模式与该品种的形态学特征完全一致。本研究认为该基因在西葫芦种皮发育及形态建成过程中起到了重要调控作用。

关键词：西葫芦 MYB转录因子端粒结合蛋白表达分析

公牛链霉菌JPD-1可湿性粉剂的研制及对苹果斑点落叶病的防效

在线全文

学校读者我要写书评

暂无评论

中国生物防治学报 2023年第2期39卷 355-365页

作者：李小伟李兴昱薛应钰甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室兰州730070

以公牛链霉菌Streptomyces tauricus JPD-1为研究对象,苹果斑点落叶病菌Alternaria alternate ***作为靶标菌,采用混料设计试验对载体、分散剂、湿润剂、紫色保护剂进行筛选,确定了公牛链霉菌JPD-1可湿性粉剂的最佳配方:载体为硅藻土,母... 详细信息

以公牛链霉菌Streptomyces tauricus JPD-1为研究对象,苹果斑点落叶病菌Alternaria alternate ***作为靶标菌,采用混料设计试验对载体、分散剂、湿润剂、紫色保护剂进行筛选,确定了公牛链霉菌JPD-1可湿性粉剂的最佳配方:载体为硅藻土,母粉89%,分散剂羧甲基纤维素钠4%,润湿剂十二烷基磺酸钠6%,紫外保护剂羧甲基纤维素钠1%。按照此配方研制出了白色粉末状的公牛链霉菌JPD-1可湿性粉剂。质量标准测定和室内防效试验结果表明,该可湿性粉剂孢子含量为2.18×10^(8)cfu/g,杂菌率2.45%,pH 5.8,细度91.95%,干燥减量4.81%,润湿时间15.4 s,悬浮率79.67%,在25℃室温下贮藏100 d芽胞存活率为70.15%;4℃条件下贮藏100d芽胞存活率为80.41%;其对斑点落叶病菌孢子萌发的最小抑菌浓度在25~50 mg/mL,对苹果斑点落叶病的防治效果84.42%,结果表明公牛链霉菌JPD-1可湿性粉剂是一种具有较好生物防治潜力的生防制剂。

关键词：公牛链霉菌JPD-1 苹果斑点落叶病可湿性粉剂防效

基于全局敏感性分析和贝叶斯方法的WOFOST作物模型参数优化

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

农业工程学报 2016年第2期32卷 169-179页

作者：何亮侯英雨赵刚邬定荣于强国家气象中心北京100081 德国波恩大学作物科学研究组波恩D-53115 中国气象科学研究院北京100081 中国科学院地理科学与资源研究所陆地水循环与地表过程重点实验室北京100101 澳大利亚悉尼科技大学生命科学学院悉尼2007

作物模型参数的敏感性分析、标定和验证可以提高模型的效率和精准度,进而为模型应用做好准备工作。该研究结合参数全局敏感性分析方法以及贝叶斯后验估计理论的马尔科夫蒙特卡洛(Markov Chain Monte Carlo,MCMC)方法,以华北栾城站三年... 详细信息

作物模型参数的敏感性分析、标定和验证可以提高模型的效率和精准度,进而为模型应用做好准备工作。该研究结合参数全局敏感性分析方法以及贝叶斯后验估计理论的马尔科夫蒙特卡洛(Markov Chain Monte Carlo,MCMC)方法,以华北栾城站三年的冬小麦观测数据(叶面积和地上生物量)为参照,对WOFOST模型的55个品种参数进行了敏感性分析、筛选和优化。发现:1)对叶面积影响较大的参数为:生育期为0、0.5、0.6和0.75时的比叶面积、生育期为1.5时的最大光合速率、叶面积指数最大增长率;对地上干物质影响较大的参数为:生育期为1.5时的最大光合速率、生育期为0时的比叶面积、35℃时叶面积的生命周期、生育期为0时的散射消光系数、生育期为1.8时的最大光合速率、储存器官的同化物转换效率。2)潜在和雨养产量水平下,最大叶面积和地上生物量对参数的敏感性差异不大。3)马尔科夫蒙特卡洛方法(MCMC)可以对WOFOST模型品种参数较好地优化;设计的3种校正-验证方案中,第1种方案(用1998-1999年作为校正年份,1999-2000年,2000-2001年作为验证年份)模拟效果最好。4)优化后的参数,模型对潜在产量水平模拟较好,一致性指数均大于0.9,相对均方根误差小于20%;而对有水分胁迫的雨养情况下比潜在产量水平的模拟结果差,表明模型对水分胁迫的模拟不足。该研究为WOFOST模型区域应用和模型调整优化提供科学理论依据。

关键词：模型作物优化 WOFOST 全局敏感性分析 MCMC 模型参数优化

苹果树腐烂病菌拮抗放线菌JPD-1的筛选及鉴定

在线全文

学校读者我要写书评

暂无评论

植物保护学报 2020年第1期47卷 134-142页

作者：薛应钰李发康李培赵娜徐秉良甘肃农业大学植物保护学院甘肃省农作物病虫害生物防治工程实验室兰州730070 甘肃省临夏州森林病虫害防治检疫站临夏731100

为筛选对苹果树腐烂病菌Valsa mali具有拮抗作用的放线菌,从苹果树根际土壤中分离获得放线菌,利用平板对峙法和生长速率法筛选拮抗活性较高的菌株,通过菌株培养特征、形态特征、生理生化特性及16S rDNA序列分析确定其分类地位,并采用离... 详细信息

为筛选对苹果树腐烂病菌Valsa mali具有拮抗作用的放线菌,从苹果树根际土壤中分离获得放线菌,利用平板对峙法和生长速率法筛选拮抗活性较高的菌株,通过菌株培养特征、形态特征、生理生化特性及16S rDNA序列分析确定其分类地位,并采用离体枝条烫伤接种法测定所筛选获得的拮抗菌株对苹果树腐烂病的防效。结果表明,从苹果树根际土壤中共分离获得28株放线菌,其中菌株JPD-1对苹果树腐烂病菌的拮抗作用最强,抑制率为72.50%,该菌在高氏合成1号培养基上生长最好,培养7 d时菌落生长旺盛,气生菌丝白色,基生菌丝深粉红色,无可溶性色素产生;经形态特征观察、生理生化试验及16S rDNA序列分析,将菌株JPD-1鉴定为公牛链霉菌Streptomyces tau‐ricus。菌株JPD-1发酵滤液对苹果树腐烂病菌的抑制率及对离体枝条上苹果树腐烂病的防效均随稀释倍数的增大而减弱,发酵滤液原液使苹果树腐烂病菌菌丝膨大、末端畸形,抑制率为78.14%,对离体枝条上苹果树腐烂病的防效为76.37%。表明分离到的放线菌菌株JPD-1可以用作苹果树腐烂病的生防材料,具有很好的开发及应用前景。

关键词：苹果树腐烂病菌放线菌鉴定离体枝条防效

在线全文

学校读者我要写书评

暂无评论

中国作物生长模拟监测系统构建及应用

农业工程学报 2018年第21期34卷 165-175,312页

作者：侯英雨何亮靳宁郑昌玲刘维张蕾国家气象中心北京100081 西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室杨凌712100

该文系统阐述了中国作物生长模拟监测系统(CropGrowthSimulatingandMonitoringSysteminChina,CGMS-China)的构建方法及其在国家级农业气象业务中的应用。CGMS-China是基于WOFOST、Oryza2000、WheatSM、ChinaAgroys 4个作物模型构建的系统,在作物长势监测评估、农业气象灾害影响评估、作物产量预报等农业气象业务中均有应用。该系统可进行作物长势监测、产量预报、农业气象灾害影响评估。利用CGMS-China模拟输出的地上生物量、叶面积指数、穗质量,建立作物长势评估指标,可对小麦、玉米、水稻进行实时长势监测与评估。通过CGMS-China对2014年8月中旬华北黄淮夏玉米的干旱产量损失评估和2016年6月22日早稻高温热害的产量损失预估表明,CGMS-China对农业气象灾害影响评估的效果较好。利用CGMS-China对2014年冬小麦主产省进行产量预报,各省的平均预报相对误差为7%。与此同时,在CGMS-China中利用遥感数据同化方法,对山西洪洞县进行产量预报,预报相对误差小于11%。该系统在国家级农业气象业务中具有良好的应用前景。

关键词：模型气象遥感作物长势监测农业气象灾害产量预报同化