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Peripheral mitochondrial DNA as a neuroinflammatory biomarker for major depressive disorder

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Neural Regeneration Research 2025年第6期20卷 1541-1554页

作者： Jinmei Ye Cong duan Jiaxin Han Jinrong Chen Ning Sun Yuan Li Tifei Yuan Daihui Peng Division of Mood Disorder Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry First Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Shanghai Key Laboratory of Psychotic Disorders Brain Health InstituteNational Center for Mental DisordersShanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

In the pathogenesis of major depressive disorder, chronic stress-related neuroinflammation hinders favorable prognosis and antidepressant response. Mitochondrial DNA may be an inflammatory trigger, after its release from stress-induced dysfunctional central nervous system mitochondria into peripheral circulation. This evidence supports the potential use of peripheral mitochondrial DNA as a neuroinflammatory biomarker for the diagnosis and treatment of major depressive disorder. Herein, we critically review the neuroinflammation theory in major depressive disorder, providing compelling evidence that mitochondrial DNA release acts as a critical biological substrate, and that it constitutes the neuroinflammatory disease pathway. After its release, mitochondrial DNA can be carried in the exosomes and transported to extracellular spaces in the central nervous system and peripheral circulation. Detectable exosomes render encaged mitochondrial DNA relatively stable. This mitochondrial DNA in peripheral circulation can thus be directly detected in clinical practice. These characteristics illustrate the potential for mitochondrial DNA to serve as an innovative clinical biomarker and molecular treatment target for major depressive disorder. This review also highlights the future potential value of clinical applications combining mitochondrial DNA with a panel of other biomarkers, to improve diagnostic precision in major depressive disorder.

关键词： biomarker cytokine exosomes inflammasome major depressive disorder microglia mitochondrial DNA mitochondrial dysfunction neuroinflammation Toll-like receptor

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思茅松SSR-PCR反应体系优化研究

西南林业大学学报（自然科学） 2018年第5期38卷 34-37页

作者：王大玮保云莹唐红燕段安安蔡年辉许玉兰周军李思广西南林业大学云南省高校林木遗传改良与繁育重点实验室云南昆明650224 云南林业职业技术学院云南昆明650224 普洱市林业科学研究所云南普洱665099 云南省林业科学院云南昆明650201

以思茅松杂交亲本JG1号基因组DNA为模板,利用正交设计实验对影响SSR-PCR反应体系的主要因子进行了优化,建立了一套最佳思茅松SSR-PCR反应体系。结果表明:在25μL最佳反应体系中,各组分最佳含量分别为Mg^(2+) 2.0 mmol/L、DNA 60 ng、引... 详细信息

以思茅松杂交亲本JG1号基因组DNA为模板,利用正交设计实验对影响SSR-PCR反应体系的主要因子进行了优化,建立了一套最佳思茅松SSR-PCR反应体系。结果表明:在25μL最佳反应体系中,各组分最佳含量分别为Mg^(2+) 2.0 mmol/L、DNA 60 ng、引物0.50μmol/L、Taq DNA聚合酶0.25U、dNTP 0.20 mmol/L。利用11份个体对此SSR反应体系进行验证,获得扩增谱带清晰且多态性较好,证明该体系稳定可靠。

关键词：思茅松 SSR-PCR 反应体系优化

Activation of adult endogenous neurogenesis by a hyaluronic acid collagen gel containing basic fibroblast growth factor promotes remodeling and functional recovery of the injured cerebral cortex

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Neural Regeneration Research 2025年第10期20卷 2923-2937页

作者： Yan Li Peng Hao Hongmei duan Fei Hao Wen Zhao Yudan Gao Zhaoyang Yang Kwok-Fai So Xiaoguang Li Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina China Rehabilitation Science Institute China Rehabilitation Research CenterBeijing Key Laboratory of Neural Injury and RehabilitationBeijingChina Center of Neural Injury and Repair Beijing Institute for Brain DisordersBeijingChina Beijing Key Laboratory for Biomaterials and Neural Regeneration School of Engineering MedicineBeihang UniversityBeijingChina Guangdong-HongKong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhouGuangdong ProvinceChina Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) GuangzhouGuangdong ProvinceChina Department of Ophthalmology and State Key Laboratory of Brain and Cognitive Sciences The University of Hong KongHong Kong Special Administration RegionChina Center for Brain Science and Brain-Inspired Intelligence Guangdong-HongKong-Macao Greater Bay AreaGuangzhouGuangdong ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Biomedical Engineering School of Biological Science and Medical EngineeringBeihang UniversityBeijingChina

The presence of endogenous neural stem/progenitor cells in the adult mammalian brain suggests that the central nervous system can be repaired and regenerated after ***,whether it is possible to stimulate neurogenesis and reconstruct cortical layers II to VI in non-neurogenic regions,such as the cortex,remains *** this study,we implanted a hyaluronic acid collagen gel loaded with basic fibroblast growth factor into the motor cortex immediately following traumatic *** findings reveal that this gel effectively stimulated the proliferation and migration of endogenous neural stem/progenitor cells,as well as their differentiation into mature and functionally integrated ***,these new neurons reconstructed the architecture of cortical layers II to VI,integrated into the existing neural circuitry,and ultimately led to improved brain *** findings offer novel insight into potential clinical treatments for traumatic cerebral cortex injuries.

关键词： adult endogenous neurogenesis basic fibroblast growth factor-hyaluronic acid collagen gel cortical remodeling functional recovery migration motor cortex injury neural circuits neural stem cells newborn neurons proliferation

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立磨机中瓷球磨矿产品粒度分布模型

中国钨业 2022年第5期37卷 38-43页

作者：赖俊全廖宁宁方鑫陈晓锋余超段安安吴彩斌江西理工大学资源与环境工程学院江西赣州341000 江西省矿业工程重点实验室江西赣州341000

基于磨矿试验与数学建模方法,以0.3~0.15 mm粒级的方解石、磁铁矿和石英3种纯矿物为研究对象,对不同矿物磨矿粒度分布特性及粒度分布数学模型拟合公式进行了研究。结果表明,当给矿体积相同时,立磨机对方解石的粉碎效果最好,石英次之,磁... 详细信息

基于磨矿试验与数学建模方法,以0.3~0.15 mm粒级的方解石、磁铁矿和石英3种纯矿物为研究对象,对不同矿物磨矿粒度分布特性及粒度分布数学模型拟合公式进行了研究。结果表明,当给矿体积相同时,立磨机对方解石的粉碎效果最好,石英次之,磁铁矿最差。运用GGS粒度分布模型和RR粒度分布模型对磨矿产品的粒度分布进行拟合,当不同矿物粒度分布相似时,GGS模型和RR模型能得到相似的拟合结果。从拟合度判定系数R2值的分布来看,GGS模型更适合用于拟合立磨机中瓷球磨矿产品的粒度分布。

关键词：瓷球立磨机 GGS粒度分布模型 RR粒度分布模型

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瓷球磨矿在行洛坑钨矿中的工业应用

中国钨业 2022年第5期37卷 50-55页

作者：钟久祥曾冲赖俊全段安安李爱民黄景华吴彩斌江西理工大学资源与环境工程学院江西赣州341000 福建宁化行洛坑钨矿有限公司福建三明365400

本研究以福建行洛坑钨矿石为对象,研究了纳米陶瓷球作为磨矿介质在钨中矿再磨中的磨矿特征,并应用于钨中矿再磨作业。研究结果表明,球磨机使用瓷球作为磨矿介质可以对钨矿石进行有效破碎,工业应用可以达到原钢球磨矿时溢流入重选的产品... 详细信息

本研究以福建行洛坑钨矿石为对象,研究了纳米陶瓷球作为磨矿介质在钨中矿再磨中的磨矿特征,并应用于钨中矿再磨作业。研究结果表明,球磨机使用瓷球作为磨矿介质可以对钨矿石进行有效破碎,工业应用可以达到原钢球磨矿时溢流入重选的产品细度要求,过粉碎现象较钢球磨矿明显改善,磨矿产品粒度分布特征更均匀,且不会有铁杂质产生,球耗从1.41 kg/t降至0.97 kg/t,电耗从8.21 kWh/t下降到5.78 kWh/t,节能降耗效果明显,取得了良好的经济效益,因此,纳米陶瓷球可以取代钢球作为钨中矿再磨的磨矿介质。

关键词：瓷球磨矿粒度分布节能降耗钨矿

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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星形AB共聚物和A均聚物的共组装模拟研究

原子与分子物理学报 2025年第5期42卷 90-94页

作者：段润宇新疆大学物理科学与技术学院乌鲁木齐830017

具有独特结构的纳米粒子在高分子材料领域具有广泛的应用.本文采用耗散粒子动力学模型系统的研究了三臂星形AB二嵌段共聚物和A均聚物共混体系的共组装相行为.通过调节聚集体中星形共聚物浓度和AB嵌段的长度比有效地控制了AB/A共混体系... 详细信息

具有独特结构的纳米粒子在高分子材料领域具有广泛的应用.本文采用耗散粒子动力学模型系统的研究了三臂星形AB二嵌段共聚物和A均聚物共混体系的共组装相行为.通过调节聚集体中星形共聚物浓度和AB嵌段的长度比有效地控制了AB/A共混体系中聚集体的共组装形貌.预测了一些有趣的结构,比如,具有较厚壳层的核-壳-补丁结构、具有壳层的且中心凹陷的圆盘状结构以及不规则椭球的多室结构.本文还发现对于具有较长A嵌段的星形链,增大A均聚物在聚集体中的浓度能够消除聚集体内B嵌段形成的核;对于具有较短A嵌段的星形链,少量的A均聚物能够诱导聚集体共组装成具有长条状核的多室纳米粒子.

关键词：耗散粒子动力学星形二嵌段共聚物共组装纳米粒子

Advances in genotypic antimicrobialresistance testing: a comprehensive review

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Science China Life Sciences 2025年第1期 130-143页

作者： Boheng duan Xianjun Zeng Junping Peng Huan Kui College of Nanchang University Department of Imaging The Second Affiliated Hospital of Nanchang University NHC Key Laboratory of Systems Biology of Pathogens National Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and MultimorbidityNational Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College

Antimicrobial resistance(AMR) represents a substantial threat to global public health, complicating the treatment of common infections and leading to prolonged illness and escalated healthcare expenses. To effectively combat AMR, timely and accurate detection is crucial for AMR surveillance and individual-based therapy. Phenotypic antibiotic resistance testing(AST) has long been considered the gold standard in clinical applications, serving as the foundation for clinical AMR diagnosis and optimized therapy. It has significantly contributed to ensuring patients′ health and the development of novel antimicrobials. Despite advancements in automated culture-based AST technologies,inherent limitations impede the widespread use of phenotypic AST in AMR surveillance. Genotypic AST technologies offer a promising alternative option, exhibiting advantages of rapidity, high sensitivity, and specificity. With the continuous advancement and expanding applications of genotypic AST technologies, such as microfluidics, mass spectrometry, and high-resolution melting curve analysis, new vigor has been injected into the development and clinical implementation of genotypic AST technologies. In this narrative review, we discuss the principles, applications, and advancements of emerging genotypic AST methods in clinical settings. The comprehensive review aims to highlight the significant scientific potential of emerging genotypic AST technologies in clinical AMR diagnosis, providing insights to enhance existing methods and explore novel approaches.

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截干处理对刺槐表型性状及内源激素含量的影响

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分子植物育种 2023年第8期21卷 2738-2745页

作者：韩超毕伟段安安孙宇涵苏立琢杨志恒牛东升王红生刘佳平韩佩尧刘晓迪李云北京林业大学生物科学与技术学院北京林木分子设计育种高精尖创新中心国家林业与草原局刺槐工程技术研究中心林木育种国家工程实验室北京100083 西南林业大学林学院昆明650224 凌海市生态建设发展中心凌海121228 吉县刺槐种子园吉县042200 吉县林业局种苗站吉县042200

为了解决刺槐(Robinia pseudoacaia L.)种子园内的成年刺槐采种树产种率低、种子品质差、结果部位上移、树势下降等问题,本研究以辽宁凌海和山西吉县种子园内的刺槐采种树为研究对象,对刺槐采种树进行不同高度(0.8, 1.0, 1.2 m)和不同年... 详细信息

为了解决刺槐(Robinia pseudoacaia L.)种子园内的成年刺槐采种树产种率低、种子品质差、结果部位上移、树势下降等问题,本研究以辽宁凌海和山西吉县种子园内的刺槐采种树为研究对象,对刺槐采种树进行不同高度(0.8, 1.0, 1.2 m)和不同年份(2016年, 2017年)的截干处理,于2018年对截干的刺槐采种树进行表型性状调查,并对其叶片内的生长素(IAA)、脱落酸(ABA)、赤霉素类(GA3, GA4, GA7)和玉米素(ZT)等多种内源激素含量进行测定。结果显示,凌海基地不同截干高度的刺槐采种树与未截干的采种树在当年生枝条长度和当年生枝条基径之间均存在显著性差异,内源激素的含量也存在明显差异。吉县基地不同年份与未截干刺槐采种树的当年生枝条长度和当年生枝条基径之间差异显著,不同的激素含量也存在显著差异。不同截干高度和不同截干年份对当年生枝条长度和当年生基径均有显著促进作用,1.0 m的截干高度更有利于脱落酸(ABA)含量的上升;1.2 m的截干高度能够显著提高赤霉素(GA4)含量的上升,有利于采种树的营养生长;0.8 m的截干高度能够显著降低玉米素(ZT)含量。不同的截干措施对植物的表型性状和植物激素的含量分析为刺槐种子园矮化经营和树体营养生长和生殖生长调控提供依据。

关键词：刺槐种子园截干采种树表型