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Variation in needle and cone traits in natural populations of Pinus yunnanensis

Journal of Forestry Research 2016年第1期27卷 41-49页

作者： Yulan Xu Keith Woeste Nianhui Cai Xiangyang Kang Genqian Li Shi Chen anan duan Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China College of ForestrySouthwest Forestry University Kunming 650224 China The State Engineering Laboratory for Tree Breeding College of Biological Science and Biotechnology Beijing Forestry University Beijing 100083 China USDA Forest Service Hardwood Tree Improvement and Regeneration Center at Purdue University West LafayetteIN 47906 USA

Pinus yunnanensis Franch. is a major component of coniferous forests in southwestern China. Little is known about its intraspecific variation. Morphological variations in needle and cone traits of P. yunnanensis were analyzed to quantify variability among and within natural populations. Seven traits were measured on 10 needles collected from 30 trees in each of the 18 sampled populations of P. yunna- nensis. Four cone traits were measured in 221 individual trees from nine populations. The results showed that there were significant differences （p 〈 0.01） both among popu- lations and within populations in each needle and cone trait. The proportion of phenotypic variation of nearly all needle and cone traits was over 50 % within populations, which showed trees within populations accounted for a majority of the total variation. The needle traits showed higher vari- ability within population than cone traits. Variability in theneedle traits was correlated with geo-climatic parameters （longitude, latitude, altitude, temperature, and precipitation）. Needle length and the ratio of needle length to fascicle sheath length showed clinal variation in response to latitudinal and altitudinal gradients. A hierarchical classification of all populations based on needle traits led to the formation of four major groups. The findings provide important genetic information for the evaluation of variation. Moreover, it will assist in management of genetic diversity of P. yunnanensis.

关键词： Genetic diversity Conservation Yunnanpine Cone morphology Needle morphology

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Variation in seed and seedling traits and their relations to geo-climatic factors among populations in Yunnan Pine(Pinus yunnanensis)

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Journal of Forestry Research 2016年第5期27卷 1009-1017页

作者： Nianhui Cai Yulan Xu Shi Chen Bin He Genqian Li Yue Li anan duan Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China College of ForestrySouthwest Forestry University The State Engineering Laboratory for Tree Breeding College of Biological Science and BiotechnologyBeijing Forestry University

Differences in seed germination and seedling growth among populations of Pinus yunnanensis Franch. were quantified to provide information for use in the regeneration, reforestation, and improvement of this species. Seed germination and seedling growth traits at the nursery stage were investigated at the population level. We evaluated relationships between pairs of measured traits as well as the association between measured traits and geo- climatic variables including longitude, latitude, elevation, temperature and precipitation. Seedling growth showed significant differences although a low magnitude of variation was found in the seed germination traits among the populations. Some populations with strong and stable growth （e.g., the Baoshan population） may be selected as superior seed sources for regeneration and reforestation. Germination percentage was negatively correlated with elevation, and positively with precipitation. In addition, germination index, vigor index and seedling height were positively correlated with precipitation. Seed germination index, cotyledon number and length, and seedling diameter were significantly and positively correlated with seed weight. Strong correlations between pairs of traits will be helpful in early evaluations for the selection of certain prominent traits.

关键词： Biomass Early evaluation Seed weightcotyledon - Yunnan Pine (Pinus yunnanensis Franch.)

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Genetic structure of needle morphological and anatomical traits of Pinus yunnanensis

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Journal of Forestry Research 2016年第1期27卷 13-25页

作者： Yujie Huang Jianfeng Mao Zhiqiang Chen Jingxiang Meng Yulan Xu anan duan Yue Li National Engineering Laboratory for Forest Tree Breeding Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education College of Biological Sciences and Technology Beijing Forestry University Key Laboratory for Forest Resources Conservation and the Southwest Mountains of China Ministry of Education Southwest Forestry University

Pinus yunnanensis Franch. is an particular conifer tree species in Yunnan-Guizhou plateau in south- west China. The morphological and anatomical traits of needles are important to evaluate geographic variation and population dynamics of conifer species. Seedlings from seven populations of P. yunnanensis were analyzed, look- ing at 22 morphological and anatomical needle traits. The results showed that variations among and within popula- tions were significantly different for all traits and the variance components within populations were generally higher than that among populations in the most tested needle traits. The proportions of three-needle fascicle were significantly different among populations. The traits related to needle size in both morphology and anatomy were positive with latitude and negative with annual temperature and precipitation. Ratio indices, including mesophyllarea/vascular bundle area, mesophyll area/resin canals area, vascular bundle area/resin canals area and mesophyU area/（resin canals area and vascular bundle area）, were negatively correlated with elevation and positively corre- lated with the annual mean temperature, showing some fitness feature for the populations. Needle traits were more significantly correlated with longitude than with other four environmental factors. Needle length was significantly correlated with almost all environmental factors. First four principal components accounted for 81.596 % of the variation with eigenvalues 〉1; the differences among populations were mainly dependent on needle width, stomatal density, section areas of vascular bundle, total resin canals, and mesophyll, as well as area ratio traits. Seven populations were divided into three categories by Euclidean distance. Variations in needle traits among the populations have shown systematic microevolution in terms of geographic impact on P. yunnanensis. This study would provide empirical data to characterize adaptation and genetic variation of P. yunnanens

关键词： Genetic structure Needle Morphologicaland anatomical traits Geographic population Pinusyunnanensis

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Germination and early seedling growth of Pinus densata ***

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Journal of Forestry Research 2016年第2期27卷 283-294页

作者： Yulan Xu Nianhui Cai Bin He Ruili Zhang Wei Zhao Jianfeng Mao anan duan Yue Li Keith Woeste Key Laboratory for Forest Resources Conservation and Usein the Southwest Mountains of China College of ForestrySouthwest Forestry UniversityKunming 650224China The State Engineering Laboratory for Tree Breeding Collegeof Biological Science and BiotechnologyBeijing Forestry UniversityBeijing 100083China State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of ScienceBeijing 100093China USDA Forest Service Hardwood Tree Improvement and Regeneration Center at Purdue University West LafayetteIN 47906USA

We studied seed germination and early seedling growth of Pinus densata to explore the range of variability within the species and to inform afforestation practices. Phenotypes were evaluated at a forest tree nursery under conditions that support Pinus yunnanensis, one of the presumed parental species of P. densata. Seeds were col- lected from 20 open-pollinated trees within each of eight autochthonous populations representing the natural distri- bution of P. densata in China to assess variation in ger- mination traits and early seedling growth, and to examine the relationships among these traits. Results showed that seeds from all populations germinated and seedlings established successfully. There were significant differences among populations in 13 of 14 traits evaluated. Seed germination and early seedling growth were strongly related to seed size and seed weight. Bigger seeds germi- nated earlier and faster than small seeds, and seedling size was positively correlated with seed size. Some germination traits were strongly and significantly correlated with cli- matic variables associated with the provenance of the studied populations. Based on these observations, we conclude there were large, significant, and biologically important differences among P. densata populations in seed germination and seedling growth traits. The observed variability probably reflects a high degree of adaptive dif- ferentiation among populations that is likely to be relevant for future afforestation.

关键词： Afforestation Conifer Gaoshan pine Provenance Seed germination Seed size Seedling vigor

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不同地理种源云南松幼苗生物量分配及其异速生长

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北京林业大学学报 2019年第4期41卷 41-50页

作者：李鑫李昆段安安崔凯高成杰西南林业大学林学院云南昆明650224 中国林业科学研究院资源昆虫研究所云南昆明650224

【目的】云南松地理种源间存在着丰富的遗传变异,研究不同种源间云南松子代幼苗生物量及其异速生长,以期深入了解云南松幼苗生物量分配在不同种源间的变异。【方法】通过容器植苗,采用单因素随机区组设计,比较了9个地理种源云南松子代... 详细信息

【目的】云南松地理种源间存在着丰富的遗传变异,研究不同种源间云南松子代幼苗生物量及其异速生长,以期深入了解云南松幼苗生物量分配在不同种源间的变异。【方法】通过容器植苗,采用单因素随机区组设计,比较了9个地理种源云南松子代幼苗生物量及其分配的差异及其与种源地理、气候因子的关系,并运用标准化主轴回归分析了云南松幼苗各器官在种源间的异速生长关系。【结果】云南松幼苗生物量及其分配在种源间具有显著差异,其中永仁种源茎和叶以及总生物量最大,但根生物量及其分配比最小,云龙种源各器官生物量和总生物量较小,但具有最大的叶生物量分配比。生物量及其分配受种源地理气候因子不同程度的影响,其中以海拔和年均温影响较大。云南松各器官间的异速生长关系在种源间发生了显著变化,同样的情况在器官生物量与个体大小之间也有发生。【结论】尽管物种相同,云南松幼苗各器官生物量在种源间的分配关系无一致的协同变化规律,体现了云南松子代幼苗因不同种源地理气候因子长期影响而形成生物量分配模式上的遗传变异。

关键词：云南松生物量分配异速生长种源遗传变异

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河北杨优树根萌条诱导及嫩枝扦插的研究

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西北林学院学报 1988年第1期 9-16页

作者：段安安西北林学院林学系

本文报导用根段促萌及嫩枝杆插方法进行河北杨成年优树的初繁试验。结果表明,根萌条诱导所需的有效积温(≥5℃)至少在206℃;嫩枝生根所需的有效积温(≥5℃)至少在200℃。根段的萌条均属皮部萌条类型;嫩枝的生根均属皮部生根类型。此试... 详细信息

本文报导用根段促萌及嫩枝杆插方法进行河北杨成年优树的初繁试验。结果表明,根萌条诱导所需的有效积温(≥5℃)至少在206℃;嫩枝生根所需的有效积温(≥5℃)至少在200℃。根段的萌条均属皮部萌条类型;嫩枝的生根均属皮部生根类型。此试验方法可为河北杨无性系对比及筛选提供良好的优树初繁材料。

关键词：河北杨优树初繁根段萌条嫩枝扦插

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Preparation and applications of calcium ferrite as a functional material:A review

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 292-310页

作者： Xiuli Han Bowen duan Lei Liu Shilong Fang Weiwei Wang College of Mining Engineering North China University of Science and TechnologyTangshan 063210China Collaborative Innovation Center of Green Development and Ecological Restoration of Mineral Resources of Hebei Province Tangshan 063210China Key Laboratory of Mining Development and Security Technology of Hebei Province Tangshan 063210China

Calcium ferrite(CF)is recognized as a potential green and efficient functional material because of its advantages of magnetism,electrochemistry,catalysis,and biocompatibility in the fields of materials chemistry,environmental engineering,and ***-fore,the obtained research results need to be systematically summarized,and new perspectives on CF and its composite materials need to be *** on the presented studies of CF and its composite materials,the types and structures of the crystal are *** addition,the current application technologies and theoretical mechanisms with various properties in different fields are ***,the various preparation methods of CF and its composite materials are elaborated in *** importantly,the advantages and disadvantages of the synthesis methods of CF and its composite materials are discussed,and the existing problems and emerging challenges in practical production are ***,the key future research directions of CF and its composite materials have been prospected from the potential application technologies to provide references for its synthesis and efficient utilization.

关键词： calcium ferrite mineral functional materials preparation application perspectives

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发挥良种在云南林业产业化中的作用

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西南林学院学报 1999年第1期19卷 62-66页

作者：段安安李桐森西南林学院资源学院

云南属我国四大林区之一．林业在经济发展中占有主导地位．林业产业化的关键在于可持续的后备森林资源，而后备资源培育的质量和数量取决于选育和应用良种的深度和广度．从国外林业发展的经验可知，持续选用良种已在生产中发挥了巨大作... 详细信息

云南属我国四大林区之一．林业在经济发展中占有主导地位．林业产业化的关键在于可持续的后备森林资源，而后备资源培育的质量和数量取决于选育和应用良种的深度和广度．从国外林业发展的经验可知，持续选用良种已在生产中发挥了巨大作用．首先应转变人们依靠自然更新、随便采种的观念，重视选育和采用良种、集约经营．国家应加强科技投入，试验示范，以点带面．科技人员应深入实际，调查研究，解决当地主要树种的良种和培育问题．采用多系造林，保持良种群体遗传多样性．长期坚持良种良法来培育后备森林资源，实现林业产业的可持续发展．

关键词：林业产业化良种资源培育云南

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宁夏枸杞雄性不育基因ms2的克隆及表达分析

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分子植物育种 2018年第10期16卷 3127-3134页

作者：程慧付海辉周军曹有龙李彦龙秦垦戴国礼段安安段淋渊西南林业大学林学院昆明650224 国家枸杞工程技术研究中心银川750002 北方民族大学银川750021

本研究以宁夏枸杞为材料,根据GenBank上已经登录的该基因的保守区设计简并引物,通过RTPCR扩增获得基因保守片段,利用RACE技术获得该基因3'端序列。最终,经拼接后得到一个长约1 802 bp的ms2基因片段(登录号为JQ341412.1),终止密码子为TAG... 详细信息

本研究以宁夏枸杞为材料,根据GenBank上已经登录的该基因的保守区设计简并引物,通过RTPCR扩增获得基因保守片段,利用RACE技术获得该基因3'端序列。最终,经拼接后得到一个长约1 802 bp的ms2基因片段(登录号为JQ341412.1),终止密码子为TAG,3'端包含非编码区和Poly A尾巴。相应编码基因片段527个氨基酸的肽段。生物信息学分析表明,ms2基因序列中含有NAD(P)H区域,该区域含有显著的TGXXGXXG结构单元,以及微体结合信号区域。从第44个氨基酸到第346个氨基酸为NAD结合区,从第459个氨基酸到第517个氨基酸为雄性不育C-末端区。同源性检索表明,ms2基因片段氨基酸序列与亚麻、葡萄、大豆、拟南芥和青菜的同源性分别为71%、71%、72%、65%和66%。系统进化树分析表明,宁夏枸杞ms2基因与葡萄的亲缘关系最近,与毛果杨、亚麻以及十字花科的植物聚为一类,而与江南卷柏的亲缘关系最远。RT-PCR分析表明,ms2基因在可育花蕾的2、3时期表达非常强,而在不育花蕾的2、3时期表达较弱。

关键词：宁夏枸杞 ms2 克隆表达分析

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基于DSC技术的不同榛树抗寒能力比较分析

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西南林业大学学报（自然科学） 2017年第2期37卷 19-24页

作者：王德新向建英段安安李丽萍西南林业大学云南生物多样性研究院云南昆明650224 西南林业大学研究生院云南昆明650224 西南林业大学国家高原湿地研究中心云南昆明650224

为了解不同地区榛树抗寒能力的差异,本研究选择了平榛、华榛和维西榛3种榛树为研究对象,采用差式扫描量热法(DSC),结合采样地点气候情况对其进行抗寒性分析。结果表明:抗寒能力最强的是平榛,其次是维西榛,最弱的为华榛;维西榛和华榛采... 详细信息

为了解不同地区榛树抗寒能力的差异,本研究选择了平榛、华榛和维西榛3种榛树为研究对象,采用差式扫描量热法(DSC),结合采样地点气候情况对其进行抗寒性分析。结果表明:抗寒能力最强的是平榛,其次是维西榛,最弱的为华榛;维西榛和华榛采集地点年均最高、最低气温相仿,但从试验数据看,维西榛的抗寒能力却与平榛相似,其中平榛的热焓值为2.398 J/g,吸热峰值温度为2.63℃;维西榛的热焓值为2.558 J/g,吸热峰值温度为2.26℃,依据DSC峰形图、灵敏度3D图等指标也可推断维西榛的抗寒能力应与平榛相近。因此,满足正态分布曲线图的植物是具有抗寒能力的;植物热焓值的数值越小,代表植物的抗寒能力越强;灵敏度3D图形显示,高灵敏度数值所占比例越高的植物,抗寒能力就越强。

关键词： DSC技术抗寒能力榛树热焓值

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