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Journal of Plant Ecology 2019年第2期12卷 216-227页

作者： Marlene I.Bär lamas anal.a l.Carrera Mónica B.Bertiller Instituto Patagónico para el Estudio de los Ecosistemas Continentales(IPEEC)-CONICET CCT CENPAT Boulevard Brown 29159120 Puerto MadrynChubutArgentina Facultad de Ciencias Naturales Universidad Nacional de la Patagonia San Juan BoscoBoulevard Brown 37009120 Puerto MadrynChubutArgentina

Aims Plants play an important role in ecosystem *** meaning of trait variation in wide environmental gradients is well known but is scarcely known across narrow *** analyze the variation of morphological,physical and chemical traits of dom-inant plant species and the potential rates of dry mass loss and N release/immobilization during senesced leaf decomposition of these species across a narrow aridity gradient,and to identify indicative traits useful to set species functional groups sharing decomposition *** We analyzed the variation of morphological,physical and chemical traits(specific leaf area,seed mass,N and soluble phenols in green and senesced leaves,plant height)in dominant plant species at 12 sites across an aridity gradient in northern Patagonia,*** collected senesced leaves of each plant species at each site and used them to estimate the poten-tial rates of dry mass loss and N release/immobilization from decomposing senesced leaves in a microcosm *** analyzed the variation of plant traits and decomposition rates across the aridity *** grouped plants species accord-ing to growth forms(perennial grasses,deciduous shrubs,ever-green shrubs)and different combinations of morpho-physical and chemical traits of green and senesced leaves and compared the potential rates of dry mass loss and N release/immobiliza-tion during leaf decomposition among these groups delimited by each grouping *** Findings Plant traits did not vary across the aridity *** potential rate of dry mass loss was positively related to aridity,while the potential rate of N release/immobilization did not vary across the *** species by separately morpho-physical and chemical traits resulted in a large overlapping in mean values of decomposition rates among *** contrast,plant groupings based on growth forms and those including all morpho-physical and chemical traits of green or senesced le

关键词： mass loss morpho-physical and chemical plant traits N release/immobilization rate plant growth forms

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Comparative Morpho-Biochemical Responses of Sunflower lines Sensitive and Tolerant to Water Stress

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American Journal of Plant Sciences 2013年第12期4卷 156-167页

作者： Andrea Andrade Ana Vigliocco Sergio Alemano anal.a l.anes Guillermina Abdala Departamento de Ciencias Naturales Facultad de Ciencias Exactas Físico-Químicas y Naturales Universidad Nacional de Río Cuarto Córdoba Argentina Departamento de Ciencias Naturales Facultad de Ciencias Exactas Físico-Químicas y Naturales Universidad Nacional de Río Cuarto Córdoba Argentina.

Water stress is likely the most important factor that adversely affects plant growth and development. In our study two inbred lines of sunflower (Helianthus annuus l.) with contrasting behavior to water deficit, the inbred lines B59, sensitive, and B71, tolerant, were subjected to water stress and compared in terms of growth parameters, relative water content, photosynthetic pigments and osmo-compatibles contents. Mannitol treatment generated a moderate water stress which resulted in a relative water content decline in both lines. In aerial part of B59 and B71 stressed-seedlings, a dramatic reduction of osmotic potential was observed, although in B71 the osmotic potential was 1.8-fold lower than in B59. The B71 line recovered the osmotic potential to control values after 8 d of re-watering. Several morphological parameters were altered by the water stress. Shoot and root relative fresh weight decreased in both lines under water stress, although B71 showed a minor drop. However, the root was less affected than the shoot in stress condition and recovery period. Water stress was affected in a greater proportion shoot dry weight (DW) than root DW in B59 and B71 lines. Nevertheless, a significant increase in root DW/shoot DW ratio was observed in stressed seedlings. A higher reduction in the individual leaf area was observed in B71 line under stress. The basal chlorophyll content was higher in the tolerant line B71 than in the sensitive B59, and carotenoids showed a high content in B71 control and stressed seedlings. Under water stress, the aerial and root proline content increased in the tolerant B71 line. Glycinebetaine decreased significantly in the aerial part of both lines in response to water stress indicating that this compound is not the main osmo-regulator. In conclusion, B71 has suitable mechanisms to enable it to respond more effectively to moderate water stress.

关键词： Growth Parameters Osmo-Compatibles Solutes Photosynthetic Pigments Sunflower Water Stress

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<i>In Vitro</i>Propagation and Recovery of Eight Apple and Two Pear Cultivars Held in a Germplasm Bank

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American Journal of Plant Sciences 2017年第9期8卷 2238-2254页

作者： analí lizárraga Marga Fraga Javier Ascasíbar María luz González Department of Plant Physiology University of Santiago de Compostela Santiago de Compostela Spain Cultigar Brión Spain Centro de Investigaciones Agrarias de Mabegondo INGACAl-CIAM Abegondo Spain

Eight apple (Malus x domestica) and two pear (Pyrus communis) traditional cultivars of commercial interest were selected from the germplasm bank CIAM (Centro de Investigaciones Agrarias de Mabegondo, NW of Spain) one of the most important collections of The Iberian Peninsula. In order to define the establishment protocol, apical buds 1.0 - 1.5 millimetre length of the selected cultivars were established in solid mineral medium Murashige and Skoog (MS) with modified vitamins (thiamine 10X) and supplemented with 1 mg·l-1 6-benzyladenine (BA), 0.2 mg·l-1 gibberellic acid (GA3) and 0.3 mg·l-1 of indole-3-butyric acid (IBA). The highest shoot initiation rates were achieved with apple cultivar “Principe Grande” (55%) and “Barburiña” pear cultivar (50%);the lowest rate was obtained with apple cultivar “Camoesa” (11%). Initiation rates for shoots culture varied between 22% and 37% for the other cultivars. The cytokinins BA, zeatin, 2-isopentyladenine (2iP) and thidiazuron (TDZ) were tested at concentrations of 0, 0.25, 0.5 and 1 mg·l-1 in the absence or presence of IBA (0.1 mg·l-1) for the multiplication step. For all cultivars, the highest multiplication rates were obtained with medium supplemented with TDZ and also in media containing BA. The in vitro rooting success of shoots in medium with 0.1 mg·l-1 IBA without cytokinins, and the survival rate of plantlets acclimatized under greenhouse conditions depended on the genotype of the different cultivars. In vitro propagation of these ten traditional apple and pear cultivars in NW Spain has not previously been reported.

关键词： Apple Shoots Pear Shoots Axillary Bud Multiplication Cytokinin Gene Bank

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Temperature-Programmed Reduction and Dispersive X-Ray Absorption Spectroscopy Studies of CeO<sub>2</sub>-Based Nanopowders for Intermediate-Temperature Solid-Oxide Fuel Cell Anodes

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Materials Sciences and Applications 2019年第10期10卷 631-642页

作者： Marina S. Bellora Joaquí n Sacanell Cristiá n Huck-Iriart analí a l. Soldati Susana A. larrondo Diego G. lamas CONICET/laboratorio de Cristalografía Aplicada Escuela de Ciencia y Tecnología Universidad Nacional de General San Martín San Martín Argentina Departamento de Física de la Materia Condensada Gerencia de Investigación y Aplicaciones Centro Atómico Constituyentes Comisión Nacional de Energía Atómica San Martín Argentina Instituto de Nanociencia y Nanotecnología (INN) CNEA-CONICET San Martín and San Carlos de Bariloche Argentina UNIDEF-CONICET-MINDEF Departamento de Investigaciones en Sólidos CITEDEF Villa Martelli Argentina Instituto de Investigación e Ingeniería Ambiental Universidad Nacional de General San Martín San Martín Argentina

In this work, we study the influence of the average crystallite size and dopant oxide on the reducibility of CeO2-based nanomaterials. Samples were prepared from commercial Gd2O3-, Sm2O3- and Y2O3-doped CeO2 powders by calcination at different temperatures ranging between 400°C and 900°C and characterized by X-ray powder diffraction, transmission electron microscopy and BET specific surface area. The reducibility of the samples was analyzed by temperature-programmed reduction and in situ dispersive X-ray absorption spectroscopy techniques. Our results clearly demonstrate that samples treated at lower temperatures, of smallest average crystallite size and highest specific surface areas, exhibit the best performance, while Gd2O3-doped ceria materials display higher reducibility than Sm2O3- and Y2O3-doped CeO2.

关键词： Crystallite Size CeO2-Based Nanomaterials Sm2O3

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Prevalence of refractive errors in Villa Maria,Córdoba,Argentina

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Eye Science 2016年第2期31卷 68-77页

作者： Victoria M.Sainchez Rafael Iribarren Santiago G.latino Victor E.Torres anal l.Gramajo Maria N.Artal Maria B.Yadarola Patricia R.Garay Jose D.luna Claudio P.Juarez Fundacion VER Centro Privado de Ojos RomagosaCordobaArgentina Department of Ophthalmology Centro Medico San LuisBuenos AiresArgentina CIES(Conicet y UNC) Facultad de Ciencias EconornicasUNCCordobaArgentina

Background:Refractive errors are among the most frequent reasons for demand of eye-care *** on refractive errors prevalence in our country are *** study has the purpose to assess the prevalence of refractive errors in an adult population of Villa Maria,Córdoba,***:The Villa Maria Eye Study is a population-based cross-sectional study conducted in the city of Villa Maria,Córdoba,Argentina from May 2008 to November ***’s aged 40+received a demographic interview and complete ophthalmological *** acuity was obtained with an ETDRS *** auto refraction was *** spherical equivalent was highly correlated between right and left eyes,so only data of right eyes are *** and hyperopia were defined with a±0.50 diopters(D)criterion and astigmatism>1 ***:This study included 646 subjects,aged 40 to 90(mean age:59.6±10.3 years old).Four hundred and sixty two(71.5%)were *** mean spherical equivalent was+0.714±2.41 D(range,-22.00 to+8.25 D)and the power of the cylinder was,on average,-0.869±0.91 D(range,0 to-6.50 D).In this sample,61.6%subjects were hyperopic,and 13.5%were *** prevalence was lower in men(9.8%versus14.9%)but this difference among genders was not statistically *** were 141 subjects(21.8%)with anisometropia greater than 1 D,and 168 subjects(26.0%)with astigmatism greater than 1 ***:The present study shows the prevalence of cycloplegic refractive errors in an adult population of *** prevalence of hyperopia was high,while myopia prevalence was very low.

关键词：屈光不正患病率玛丽亚阿根廷别墅平均年龄近视眼屈光度

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小桐子早期光诱导蛋白基因ElIP克隆及其与靶向miRNA的互作与低温下共表达分析

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西北植物学报 2024年第3期44卷 430-442页

作者：陈建南朱晓琴吕宇婧龚明云南师范大学生命科学学院生物能源持续开发利用教育部工程研究中心云南省生物质能与环境生物技术重点实验室昆明650500

【目的】作为一种喜温冷敏植物,低温严重影响小桐子的生长发育、地域分布与产量。前期工作发现12℃低温锻炼可显著提高小桐子的抗冷性,小桐子早期光诱导蛋白(ElIP)基因是低温高响应基因。为探究JcElIP在小桐子低温响应中的作用,全面了解... 详细信息

【目的】作为一种喜温冷敏植物,低温严重影响小桐子的生长发育、地域分布与产量。前期工作发现12℃低温锻炼可显著提高小桐子的抗冷性,小桐子早期光诱导蛋白(ElIP)基因是低温高响应基因。为探究JcElIP在小桐子低温响应中的作用,全面了解JcElIP的结构、调控机制、进化关系及JcElIP与miRNAs的互作关系,也为后续小桐子抗冷分子育种提供一个重要的候选基因资源。【方法】通过RT-PCR克隆了小桐子JcElIP基因并对其进行系统的生物信息学分析,采用RT-qPCR分析JcElIP基因在根、茎、叶中及12℃低温锻炼过程中的表达变化,鉴定与JcElIP互作的miRNAs,进行了12℃低温锻炼过程中的共表达分析。【结果】该基因完整的开放阅读框(ORF)为585 bp,编码194个氨基酸,蛋白的分子质量为2.04 kD,理论等电点为9.59,属于稳定的疏水碱性蛋白,具有3个疏水跨膜螺旋;三级结构主要由ɑ-螺旋和无规则卷曲组成,具有叶绿素a/b结合位点。顺式作用元件预测显示JcElIP具有脱落酸等激素响应元件。进化分析显示:小桐子JcElIP与木薯MeElIP同源性最高。RTqPCR分析显示:正常生长条件下小桐子JcElIP在根、茎、叶中的表达量无显著差异;12℃冷锻炼条件下JcElIP在叶中表达快速上调,48 h时其表达量达到对照组的64.8倍,表明JcElIP参与了小桐子对冷胁迫的响应与适应。基于小桐子降解组数据,鉴定到miR390-x、miR6476-x和novel-m0090-3p等8个miRNAs对JcElIP的表达具有调控作用;共表达分析结果表明在12℃低温锻炼过程中JcElIP的表达受miR390-x和novel-m0090-3p显著负调控。【结论】JcElIP高响应低温胁迫并与miRNAs互作,表明其在小桐子响应低温胁迫过程中发挥重要作用。

关键词：小桐子早期光诱导蛋白(ElIP)基因冷锻炼表达分析 miRNA互作

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Use of calcein and alizarin red S for immersion marking of black rockfish Sebastes schlegelii juveniles

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Chinese Journal of Oceanology and limnology 2014年第1期32卷 88-98页

作者： lü Hongjian ZHANG Xiumei XI Dan GAO Tianxiang College of Fisheries Ocean University of China College of Animal Science and Technology Southwest University

Black rockfish Sebastes schlegelii juveniles （30-40 mm total length） were immersed in a range of calcein （CAl） solutions at concentrations ranging from 50 to 250 mg/l and alizarin red S （ARS） solutions at concentrations ranging from 100 to 500 mg/l in filtered seawater （salinity 30） for 24 h. Fluorescent marks were detected in otoliths （sagittae, asteriscus）, scales, fin rays （dorsal, pectoral, ventral, anal, and caudal fin rays）, and fin spines （dorsal, ventral, and anal fin spines） after a 60-d growth experiment. With the exception of 50-100 rng/l CAl, acceptable marks were produced in the otoliths and fin spines by all concentrations of CAl and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fin spines offish immersed in 200 500 mg/l, 300-500 rag/l, and 200-500 mg/lARS, respectively. Scales and fin rays had acceptable marks at much higher concentrations （≥50 mg/l CAl, ≥300 mg/l ARS for scales and ≥50 mg/l CAl,≥200 mg/l ARS for fin rays）. The mark quality was highest （i.e., acceptable marks were observed in all sampled structures after immersion marking） in fish immersed in 150-250 mg/l CAl or 300-500 mg/lARS. In addition, there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking （P〉0.05）.

关键词： Sebastes schlegelii calcein (CAl) alizarin red S (ARS) otoliths fin rays fin spines

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Diagnosis andmanagement of floppy pouch complex

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Gastroenterology Report 2018年第4期6卷 246-256,I0001页

作者： Freeha Khan Tracy l.Hull Bo Shen Gastroenterology and Hepatology Center for Inflammatory Bowel DiseaseCleveland ClinicClevelandOH 44195USA

Restorative proctocolectomy with ileal pouch–anal.anastomosis has become the surgical treatment of choice for patients with refractory ulcerative colitis,colitis-associated dysplasia or familial adenomatous *** are various pouch disorders and associated *** pouch complex is defined as the presence of pouch prolapse,afferent limb syndrome,enterocele,redundant loop and folding pouch on pouchoscopy,gastrografin pouchogramor *** clinical presentation includes dyschezia,bloating,abdominal pain,straining or the sense of incomplete *** disorder has its own unique endoscopic,radiographic and manometry findings.A range of therapeutic options are available for the management of the various causes of a pouch.

关键词： Floppy pouch complex ileal pouch prolapse afferent limb syndrome efferent limb syndrome ulcerative colitis

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Glass Formation and Thermal Stability in Rapidly Solidified Al-Fe-V-Si Alloy with Misch Metal Additions

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Journal of Materials Science & Technology 1996年第1期12卷 11-14页

作者： Wang, JQ Zeng, MG Tseng, MK Chen, XF Dong, l Gu, SX lIAONING PROVINCE CTR anal & TESTING RES SHENYANG 110015PEOPLES R CHINA

A new amorphous Al(87.3)Fe(4.3)V(0.7)Si(1.7)Mm(6.0)(Mm: misch metal) was prepared by using melt-spinning technique. Differential scanning calorimetry (DSC), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) have been used to investigate the crystallization behaviour of this amorphous alloy. It is found that the crystallization process can be concluded as follows: amorphous --> amorphous plus fcc-Al --> fcc-Al plus Al-11(la,Ce)(3) plus i-phase (icosahedral) --> fee-Al plus Al-11(la,Ce)(3) plus i-phase plus ic-phase (icosahedral compounds). The results of microhardness measurement show that HV values are higher than those in AI-Fe-V-Si based alloy, and the maximum HV value corresponds to the coexisting of nanoscale Al particles and i-phase.

关键词： Aluminum alloys

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The lipase monolayer film self-assembly on the negatively charged poly(ethylene terephthalate) substrate

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Chinese Chemical letters 2000年第3期11卷 267-270页

作者： lin, XF He, l Xu, lH lu, DS Chen, ZC Zhejiang Univ Ctr Anal & Measurement Hangzhou 310027 Peoples R China

The PET-CO2- film was prepared and the lipase was assembled on the surface of the PET-CO2- substrate The structure at the surface and activity of lipase/PET monolayer were studied by ATR-FTIR and AFM, and other methods.

关键词： self-assembled monolayer lipase PET-CO2 substrate

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