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Chemical Research in Chinese Universities 2014年第1期30卷 119-124页

作者： popescu ana-maria CONSTANTIN Virgil Laboratory of Molten Salts llie Murgulescu Institute of Physical Chemistry of the Romanian Academy Bucharest 006021 Romania

The authors prepared,characterized and preliminary studied the properties of some neoteric solvents-ionic liquids based on choline chloride,i.e.,three ionic liquids based on the eutectics of choline chloride（2-hydroxyN,N,N-trimethylethanaminium chloride） with,respectively,urea,malonic acid and citric *** obtained mixtures were clear and colorless ionic liquids at room *** thermophysical properties,namely,density,viscosity,and electrical conductivity of these mixtures were investigated as a function of temperature within a range of 298-353 *** for the temperature dependence of the measured properties were generated and discussed in terms of Arrhenius *** the electrochemical windows of the eutectic liquids were determined.

关键词： Neoteric solvent Ionic liquid Choline chloride Malonic acid Citric acid Density Viscosity Conductivity

Electrochemical Study and Electrodeposition of Copper（I） in Ionic Liquid-reline

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Chemical Research in Chinese Universities 2013年第5期29卷 991-997页

作者： popescu ana-maria COJOCARU Anca DONATH Cristina CONSTANTIN Virgil Laboratory of Molten Salts "Hie Murgulescu" Institute of Physical Chemistry of Romanian Academy Bucharest 060021 Romania Department of Applied Physical Chemistry and Electrochemistry University Politehnica of Bucharest Bucharest 010737 Romania

The authors made the electrochemical studies on the electrode processes during copper electrodeposition with ionic liquids-reline as an electrolytic medium. Reline is a commercial name for a eutectic mixture of choline chloride and urea（1：2, molar ratio）. This new proposed method of copper deposition is an ecological one because the ionic liquids are recyclable, non-volatile and non-toxic. Cyclic voltammetry investigations were conducted at 343 and 353 K in choline chloride-urea（Reline） deep eutectic solvent with single salt CuCl（generally in 0.05--0.38 mol/L concentration range）. Electrochemical impedance spectroscopy investigations were conducted in choline chloride- urea ionic liquid with CuCI（0.05 mol/L）. We investigated both cathodic and anodic processes of Cu＋ ions at different sweep rates in this new electrolytic system based on choline chloride. We have proposed by this an alternative to the classical copper electrodeposition. Coefficient of diffusion, Do, for the system Reline-CuCl is presented and the value of D0 is 1.22× 10^-8 cm2/s.

关键词： Ionic liquid Reline Electrodeposition Cu(I) Coefficient of diffusion

Electroreduction of Tungsten Oxide（VI） in Molten Salts with Added Metaphosphate

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Chemical Research in Chinese Universities 2013年第4期29卷 771-775页

作者： MALYSHEV Victor GAB Angelina popescu ana-maria CONSTANT1N Virgil Faculty of Chemistry & Technology National Technical University of Ukraine "Kyiv Polytechnical Institute " Kyiv 05056 Ukraine" Open International University of Human Development of Ukraine Kyiv 04071 Ukraine Laboratory of Molten Salts Ilie Murgulescu" Institute of Physical Chemistry of the Romanian Academy Bucharest 006021 Roumania

Tungstate ions WO4^2- are not electrochemically active in chloride melts. Upon introduction of PO3 ions into the melt, two waves appear in the voltammograms at potentials -0.1-- -0.2 V and -0.3-- -0.5 V vs. Pb/Pb2＋ reference electrode. With a PO3- concentration ratio of 0.01〈[PO3-]/[WO42-]〈0.18, the potentiostatic electrolysis product of WO42 at the above mentioned potentials is metallic tungsten; a NaPO3 concentration increase for ratios [PO3-]/[WO42-]〉〉0.18 results in tungsten phosphide in electrolysis product. Cyclic voltammograms and dependence of half-peak potentials on electrode polarization rate indicate the irreversible character of the electrode process. Elec- trode process modeling allows us to state that the first wave in the voltammogram of KC1-NaC1-Na2WOa-NaPO3 sys- tem corresponds to tungsten oxychlorides discharge while the second wave appears due to the discharge of di- tungstate ions. In the voltammograms of Na2WOa-NaPO3 melts, reduction wave was observed at -1.1-- -1.2 V po- tentials. Proportionality of limiting current to NaPO3 concentration, constancy of ld/V1/2 ratio, and IJnFc constant ki- netic value equal to （8.3--9.5）×10-5 cm/s for steady-state wave indicate that electrode process rate is limited by elec- trochemically active particle diffusion to the electrode. Nascent ditungstate ions become electrochemically active in the overall electrode process. Charge transfer stage is reversible.

关键词： Tungsten oxide Electroreduction Molten salt Cyclic voltammetry

Urea breath test for Helicobacter pylori infection in adult dyspeptic patients: A meta-analysis of diagnostic test accuracy

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World Journal of Gastroenterology 2024年第6期30卷 579-598页

作者： Fabian Fellipe Bueno Lemos Caroline Tianeze de Castro Marcel Silva Luz Gabriel Reis Rocha Gabriel Lima Correa Santos Luís Guilherme de Oliveira Silva mariana Santos Calmon Cláudio Lima Souza ana Carla Zarpelon-Schutz Kádima Nayara Teixeira Dulciene maria de Magalhães Queiroz Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Instituto de Saúde Coletiva Universidade Federal da BahiaSalvador 40110040BahiaBrazil Campus Toledo Universidade Federal do ParanáToledo 85919-899ParanáBrazil Laboratory of Research in Bacteriology UFMGBelo Horizonte 30130-100Minas GeraisBrazil

BACKGROUND Helicobacter pylori(***)infection has been well-established as a significant risk factor for several gastrointestinal *** urea breath test(UBT)has emerged as a leading non-invasive method for detecting *** numerous studies confirming its substantial accuracy,the reliability of UBT results is often compromised by inherent *** findings underscore the need for a rigorous statistical synthesis to clarify and reconcile the diagnostic accuracy of the UBT for the diagnosis of *** *** To determine and compare the diagnostic accuracy of 13C-UBT and 14C-UBT for *** infection in adult patients with *** We conducted an independent search of the PubMed/MEDLINE,EMBASE,and Cochrane Central databases until April *** search included diagnostic accuracy studies that evaluated at least one of the index tests(^(13)C-UBT or ^(14)C-UBT)against a reference *** used the QUADAS-2 tool to assess the methodo-logical quality of the *** utilized the bivariate random-effects model to calculate sensitivity,specificity,positive and negative test likelihood ratios(LR+and LR-),as well as the diagnostic odds ratio(DOR),and their 95%confidence *** conducted subgroup analyses based on urea dosing,time after urea administration,and assessment *** investigate a possible threshold effect,we conducted Spearman correlation analysis,and we generated summary receiver operating characteristic(SROC)curves to assess ***,we visually inspected a funnel plot and used Egger’s test to evaluate publication *** both as reliable diagnostic tools in clinical *** In summary,our study has demonstrated that ^(13)C-UBT has been found to outperform the ^(14)C-UBT,making it the preferred diagnostic ***,our results emphasize the significance of carefully considering urea dosage,assessment timing,and measurement techniques for both tests to enh

关键词： Helicobacter pylori Urea breath test Diagnosis Diagnostic test accuracy Meta-analysis

Screening of Environmental Bacterial Strains for Wild Aedes Mosquito Larval Biocontrol

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Open Journal of Applied Sciences 2024年第9期14卷 2431-2442页

作者： Alexandre Paulo Machado Liliana Alves Correa Gabriel de Paula Albuquerque ana Lúcia maria Ribeiro Rosina Djunko Miyazaki Jéssica da Silva Gava Dyego Bichofre Cruz Laboratory of Microbiology Medical Faculty Federal University of Mato Grosso Mato Grosso Brazil Laboratory of Entomology Institute of Biosciences Federal University of Mato Grosso Mato Grosso Brazil

The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights the need for novel, environmentally friendly, cost-effective control strategies. This study explored the potential of environmental bacterial isolates to biocontrol wild Aedes larvae. Initially, we collected bacterial samples from infectious masses of Aedes fluviatilis larvae. The isolated bacteria were identified using biochemical, enzymatic, and molecular methods, including 16S rRNA sequencing and MALDI-TOF. Previously, Aeromonas hydrophila and Bacillus thuringiensis isolated from these infectious masses showed limited Aedes larval inhibition. Consequently, we screened additional environmental isolates from the bacteriotheque. Six isolates previously identified were tested: Chromobacterium violaceum, Enterobacter cloacae, Bacillus cereus, Bacillus sphaericus, and two strains of Bacillus thuringiensis israelensis. Among these strains, B. thuringiensis and C. violaceum exhibited significant inhibitory activities against wild Aedes larvae. Bacillus thuringiensis cultures grown under daylight conditions showed a slight ability to inhibit Aedes larvae. The potential of B. thuringiensis and C. violaceum strains studied, along with optimized culture growth conditions, will be further investigated to develop bioinsecticide products to provide safer and more sustainable alternatives for controlling larvae of Aedes mosquitoes.

关键词： Bacillus thuringiensis Chromobacterium violaceum Aedes Biocontrol Larval Inhibition

Long chikungunya?An overview to immunopathology of persistent arthralgia

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World Journal of Virology 2024年第2期13卷 48-57页

作者： Jayme Euclydes Picasky Silveira-Freitas maria Luiza Campagnolo mariana dos Santos Cortez Fabrício Freire de Melo ana Carla Zarpelon-Schutz Kádima Nayara Teixeira Campus Toledo Universidade Federal do ParanáToledoParaná85919-899Brazil Instituto Multidisciplinar em Saúde Universidade Federal da BahiaCampus Anísio TeixeiraVitória da ConquistaBahia 45029-094Brazil Programa de Pós-graduação em Biotecnologia PalotinaUniversidade Federal do Paraná-Setor PalotinaParaná85950-000Brazil Programa Multicêntrico de Pós-graduação em Bioquímica e Biologia Molecular PalotinaUniversidade Federal do Paraná-Setor PalotinaParaná85950-000Brazil

Chikungunya fever(CF)is caused by an arbovirus whose manifestations are extremely diverse,and it has evolved with significant severity in recent *** clinical signs triggered by the Chikungunya virus are similar to those of other ***,fever starts abruptly and reaches high levels,followed by severe polyarthralgia and myalgia,as well as an erythematous or petechial maculopapular rash,varying in severity and *** 40%to 60%of affected individuals report persistent arthralgia,which can last from months to *** symptoms of CF mainly represent the tissue tropism of the virus rather than the immunopathogenesis triggered by the host's immune *** main mechanisms associated with arthralgia have been linked to an increase in T helper type 17 cells and a consequent increase in receptor activator of nuclear factor kappa-Βligand and bone *** review suggests that persistent arthralgia results from the presence of viral antigens post-infection and the constant activation of signaling lymphocytic activation molecule family member 7 in synovial macrophages,leading to local infiltration of CD4+T cells,which sustains the inflammatory process in the joints through the secretion of pro-inflammatory *** term"long chikungunya"was used in this review to refer to persistent arthralgia since,due to its manifestation over long periods after the end of the viral infection,this clinical condition seems to be characterized more as a sequel than as a symptom,given that there is no active infection involved.

关键词： Chikungunya Immunopathology Inflammation process Persistent arthralgia Signaling lymphocytic activation molecule family member 7

Adding vortexing to the Maki technique provides no benefit for the diagnosis of catheter colonization or catheter-related bacteremia

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World Journal of Critical Care Medicine 2024年第1期13卷 58-64页

作者： Leonardo Lorente maria Lecuona Fernandez Adriana González-Mesa Judith Oliveras-Roura Cristina Rosado Pablo Cabrera Emma Casal Alejandro Jiménez María Luisa Mora ana Madueño Intensive Care Unit Hospital Universitario de CanariasLa Laguna 38320Spain Microbiology and Infection Control Service Hospital Universitario de CanariasLa Laguna 38320Spain Research Unit Hospital Universitario de CanariasLa Laguna 38320Spain

BACKGROUND A previous study compared vortexing and Maki techniques for the diagnosis of catheter-related bloodstream infection(CRBSI),and concluded that vortexing was not superior to Maki *** To determine whether the combined use of vortexing and Maki techniques provides profitability versus the Maki technique for the diagnosis of catheter tip colonization(CTC)and *** Observational and prospective study carried out in an Intensive Care *** with suspected catheter-related infection(CRI)and with one central venous catheter for at least 7 days were *** area under the curve(AUC)of the Maki technique,the vortexing technique and the combination of both techniques for the diagnosis of CTC and CRBSI were *** We included 136 episodes of suspected *** found 21 cases of CTC of which 10 were also CRBSI *** the 21 CTC episodes,18(85.7%)were diagnosed by Maki technique and vortexing technique,3(14.3%)only by the technique of Maki,and none only by technique of *** the 10 CRBSI episodes,9(90.0%)were diagnosed by the techniques of Maki and vortexing,1(10.0%)was diagnosed only by the technique of Maki,and none only by the technique of *** no found differences in the comparison of AUC between the technique of Maki and the combination of Maki and vortexing techniques for the diagnosis of CTC(P=0.99)and CRBSI(P=0.99).CONCLUSION The novel finding of our study was that the combined use of vortexing and Maki techniques did not provide profitability to the technique of Maki alone to CRBSI diagnosis of.

关键词： Vortexing Maki Bloodstream infection Colonization

Concentric Centrifugal Figurate Erythema as an Unusual COVID-19 Evanescent Skin Manifestation in a Patient With CD3^(+)/CD45^(+)Lymphocyte Infiltration:A Case Report

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International Journal of Dermatology and Venereology 2024年第2期7卷 115-118页

作者： Lucía Achell Achell-Nava José Luis Zaldivar-Fujigaki Rebeca Pérez-Cabeza de Vaca ana maria Espinosa García Luis Montiel López Dermatology Department National Medical Center 20 de NoviembreISSSTEMexico City 03229Mexico Biomedical Research Department National Medical Center 20 de NoviembreISSSTEMexico City 03100Mexico Clinical Pharmacology Division General Hospital of MexicoMexico City 06720Mexico Internal Medicine Department National Medical Center 20 de NoviembreISSSTEMexico City 03100Mexico

Introduction:Coronavirus disease 2019(COVID-19)may be associated with various skin *** them,figurate erythema(FE)is rare;only a few clinical reports have been ***,we reported an unusual concentric FE lesions and their immunohistochemical profile in a patient with *** presentation:A 52-year-old woman was diagnosed with *** month later,she developed asymptomatic annular erythematous concentric ring lesions on her inner right *** margin of every ring increased in coloration,and the lesions then completely resolved in 2 weeks.A biopsy revealed vasculitis of small vessels,an increase in the fluorescence intensity of CD3 and CD45,and negativity for COVID-19 ***:The results showed an unusual FE that has a clinical mixed type between erythema annulare centrifugum and erythema gyratum repens;the physiopathological mechanism in the FE due to COVID-19 is unknown;one theory involves rapid viral passage into the cutaneous vascular system that triggers an immune and inflammatory *** the results exhibited a negative COVID-19 RNA in the tissue,the CD3^(+)/CD45^(+)lymphocyte infiltration could explain the clinical pattern as a direct inflammatory response in the small vessel or as a delayed immune ***:Our findings suggest an immunohistochemical small vessel vasculitis pattern with an unusual clinical FE that differs from other types of COVID-19-associated FE or erythema reported previously.

关键词： erythema COVID-19 leukocytoclastic vasculitis skin manifestation CD3 complex CD45 antigen

Perspective on automated in vivo drug screening using the chodl mutant zebrafish line

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Neural Regeneration Research 2022年第11期17卷 2437-2438页

作者： ana-maria Oprisoreanu Center for Regenerative Therapies Dresden(CRTD) TU Dresden

Since the early 2000s, the popularity of zebrafish in in vivo drug screening has shown a substantial *** zebrafish has become an important screening tool covering a wide range of tissue-specific pathologies and diseas... 详细信息

关键词： fish vivo screening

围术期神经肌肉阻滞的管理:欧洲麻醉和重症监护学会临床指南

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中华麻醉学杂志 2024年第6期44卷 641-656页

作者： Thomas Fuchs-Buder Carolina S.Romero Heidrun Lewald Massimo Lamperti Arash Afshari ana-Marjia Hristovska Denis Schmartz Jochen Hinkelbein Dan Longrois maria Popp Hans Dde Boer Massimiliano Sorbello Radmilo Jankovic Peter Kranke 法国南锡大学医学院附属医院麻醉学、重症监护与围术期医学科法国南锡54000 巴伦西亚大学综合医院麻醉与重症监护科西班牙巴伦西亚46014 慕尼黑工业大学麻醉学与重症监护科德国慕尼黑80333 克利夫兰阿布扎比诊所麻醉学研究所阿联酋阿布扎比31250 哥本哈根大学医院小儿和产科麻醉科丹麦哥本哈根2100 哥本哈根大学医院麻醉学与重症监护科丹麦赫维德2650 布鲁塞尔自由大学Erasme医院麻醉科比利时布鲁塞尔1070 科隆大学医院麻醉学与重症监护科德国科隆50937 巴黎大学附属比查特克劳德伯纳德医院麻醉学与重症监护医学科法国巴黎75018 维尔茨堡大学医院麻醉学、危重病医学、急诊医学和疼痛医学科德国维尔茨堡97080 格罗宁根大学医院麻醉学、疼痛医学与程序性镇静与镇痛科荷兰格罗宁根9713 GZ 卡坦尼亚大学医院麻醉与重症监护科意大利卡坦尼亚95123 尼什大学医学院尼什临床中心麻醉学与重症治疗科塞尔维亚尼什18000

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(Europea... 详细信息

近来的研究表明,由于围术期神经肌肉阻滞管理不当,肌肉松弛药(肌松药)残留阻滞及其术后并发症的发生率居高不下。需要重视神经肌肉功能监测仪、肌松药及其拮抗剂的合理应用,以利于改善神经肌肉阻滞管理。欧洲麻醉和重症监护学会(European Society of anaesthesiology and Intensive Care,ESAIC)首次发布围术期神经肌肉阻滞管理临床指南,旨在为临床医生提供最佳医疗管理的循证建议,以提高围术期患者安全。本指南主要聚焦在3个临床问题:肌松药是否有助于成人气管插管?神经肌肉阻滞深度是否影响腹部手术患者的预后?肌松药残留阻滞的诊断和治疗策略有哪些?根据PICO原则(患者、干预、对照、结局;patient,intervention,comparator,outcome;PICO)构建临床问题并进行文献检索。初始纳入的临床研究为24 000篇,采用逐步筛选策略,最终临床研究88篇纳入指南分析。应用GRADE方法学(Grading of Recommendations,Assessment,Development and Evaluation,GRADE)对结局指标证据、质量等级进行评价。运用两轮德尔菲法收集专家组建议并形成统一推荐:R1推荐应用肌松药辅助气管插管(1A)。R2推荐应用肌松药以减少气管插管导致的咽部和(或)喉部损伤(1C)。R3推荐在快速顺序诱导插管(rapid sequence induction intubation,RSII)时使用短效肌松药,例如琥珀胆碱1 mg/kg或罗库溴铵0.9~1.2 mg/kg(1B)。R4推荐在需要改善外科手术条件时,加深神经肌肉阻滞深度(1B)。R5尚无足够的证据推荐深度神经肌肉阻滞可减轻术后疼痛或降低围术期并发症的发生率(2C)。R6推荐在拇内收肌处刺激尺神经,定量监测神经肌肉阻滞深度以排除肌松药残留阻滞(1B)。R7推荐应用舒更葡糖钠逆转甾体类肌松药(罗库溴铵、维库溴铵)诱导的深、中及浅度神经肌肉阻滞(1A)。R8推荐在使用新斯的明前,TOFr自行恢复至>0.2,并继续定量监测神经肌肉功能,直至TOFr超过0.9(1C)。

关键词：神经肌肉阻滞神经肌肉功能肌松药临床指南罗库溴铵琥珀胆碱外科手术定量监测