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T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway

Cellular & Molecular Immunology 2017年第6期14卷 497-510页

作者： Jose-Ignacio Rodriguez-Barbosa Carlos Fernandez-Renedo ana maria bravo moral Leo Biihler maria-Luisa del Rio Transplantation Immunobiology Section Institute of Biomedicine University of Leon and Castilla and Leon Regional Transplantation Coordination Leon University Hospital Leon Spain Department of Veterinary Clinical Sciences University of Santiago de Compostela Veterinary Faculty Lugo Spain Visceral and Transplantation Surgery Department of Surgery University Hospitals of Geneva and Faculty of Medicine Geneva Switzerland

The molecular pathways contributing to humoral-mediated allograft rejection are poorly defined. In this study, we assessed the role of the herpesvirus entry mediator/B- and T-lymphocyte attenuator （HVEM/BTLA） signalling pathway in the context of antibody-mediated allograft rejection. An experimental setting was designed to elucidate whether the blockade of HVEM/BTLA interactions could modulate de novo induction of host antidonor-specific antibodies during the course of graft rejection. To test this hypothesis, fully allogeneic major histocompatibility complex-mismatched skin grafts were transplanted onto the right flank of recipient mice that were treated with isotype control, anti-CD40L or modulatory antibodies of the HVEM/BTLA signalling pathway. The frequencies of CD4 T follicular helper （Tfh） cells （B220-, CD4＋ CXCR5＋ PD-lhigh）, extrafollicular helper cells （B220-, CD4＋ CXCR5- PD-1＋ and PD-1-） and germinal centre （GC） B cells （B220＋Fas＋ GL7＋） were analysed by flow cytometry in draining and non-draining lymph nodes at day 10 post transplantation during the acute phase of graft rejection. The host antidonor isotype-specific humoral immune response was also assessed. Whereas blockade of the CD40/CD40L pathway was highly effective in preventing the allogeneic humoral immune response, antibody-mediated blockade of the HVEM/BTLA-interacting pathway affected neither the expansion of Tfh cells nor the expansion of GC B cells. Consequently, the course of the host antidonor antibody-mediated response proceeded normally, without detectable evidence of impaired development. In summary, these data indicate that HVEM/BTLA interactions are dispensable for the formation of de novo host antidonor isotype-specific antibodies in transplantation.

关键词： BTLA (CD272) humoral immunity HVEM (TNFRSF14, CD270) T follicular helper cells transplantation

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Single Layer Centrifugation with Androcoll-E^(TM) improved progressive motility and percentage of live spermatozoa with intact acrosome of chilled stallion semen but did not have an effect on DNA integrity

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Open Journal of Animal Sciences 2012年第3期2卷 159-165页

作者： ana Luisa Costa ana Martins-Bessa ana Rebello de Andrade Tiago Guimaraes maria Rosa Rebordao Sandra Gamboa Pedro Pinto bravo maria José Correia Jorge Colaco Isabel Gaivao António Rocha Centro de Ciencia Animal e Veterinária(CECAV) University of Trás-os-Montes e Alto DouroQuinta de PradosVila RealPortugal Veterinary Degree Universty of Tras-os-Montes e Alto DouroQuinta de PradosVila RealPortugal Centro de Estudos em Ciencia Animal(CECA) Instituto de Ciencias Biomedicas Abel Salazar(ICBAS)University of PortoOportoPortugal Coimbra Higher School of Agriculture Sao Martinho do BispoCoimbraPortugal Alter Real Foundation Alter do ChaoPortugal

A significant number of stallions produce low quality ejaculates with high sensibility to chilling. Single Layer Centrifugation (SLC) with Andro-coll-ETM has been presented as an efficient method of selecting good quality spermatozoa. In the current study, changes in sperm quality (motility, viability, acrosome integrity and DNA damage) occurring during storage at 5?C for a maximum of 72 h, were investigated. For that, one ejaculate from 12 stallions was split in two aliquots: control and SLC-selected. Both aliquots were chilled and stored at 5?C and spermatozoa were evaluated for motility, viability and acrosome integrity at 24, 48 and 72 h post collection. DNA damage was evaluated at 48 h post collection using the comet assay. In the SLC-selected aliquots, there was a significant improvement in terms of progressive motility (0 h: P = 0.005;24 h: P 0.05). SLC with Androcoll-ETM improved semen quality prolonging sperm longevity of chilled semen (P = 0.012). This positive effect was more evident in ejaculates most sensitive to chilling that had a sharp decrease in motility in the first 24 h of refrigeration and for all ejaculates at 72 h post-chilling. Therefore, this method reveals to be a useful technique for selecting spermatozoa and maintain sperm quality during storage.

关键词： Stallion Sperm Single Layer Centrifugation Androcoll-ETM Membrane Integrity Comet assay Component Formatting Style Styling

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Correction to: T follicular helper expansion and humoralmediated rejection are independent of the HVEM/BTLA pathway

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Cellular & Molecular Immunology 2019年第3期16卷 314-314页

作者： Jose-Ignacio Rodriguez-Barbosa Carlos Fernandez-Renedo ana María bravo moral Leo Bühler maria-Luisa del Rio Transplantation Immunobiology Section Institute of BiomedicineUniversity of Leon and Castilla and Leon Regional Transplantation CoordinationLeon University HospitalLeonSpain Department of Veterinary Clinical Sciences University of Santiago de CompostelaVeterinary FacultyLugoSpain Visceral and Transplantation Surgery Department of SurgeryUniversity Hospitals of Geneva and Faculty of MedicineGenevaSwitzerland

In this article,one of the grating agencies requested us to incorporate the information,Spanish Government and co-funded by European Union ERDF/ESF,“Investing in your future”,in the acknowledgments *** correct acknowledgement is as follows:“This work has been supported by grants of the Spanish Ministry of Health(Fondo de Investigaciones Sanitarias,PI13/00029,Spanish Government and co-funded by European Union ERDF/ESF,“Investing in your future”),Department of Education of Castilla and Leon Regional Government(Grant#LE093U13)and Mutua Madrileña Foundation(Basic research grants 2012)to J.I.R.B.;by Miguel Servet National Program(Ministry of National Health)CP12/03063 and by Gerencia Regional de Salud GRS963/A/2014 to *** are particularly grateful to *** Alaiz for outstanding animal husbandry.”The authors regret the errors.

关键词： section. expansion husbandry

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