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Concurrent Validity of a Virtual Version of Box and Block Test for Patients with neurological Disorders

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World Journal of neuroscience 2020年第1期10卷 79-89页

作者： M. Alvarez-Rodríguez E. López-Dolado M. Salas-Monedero V. Lozano-Berrio S. Ceruelo-Abajo A. Gil-Agudo A. de los reyes-guzmán Biomechanics and Technical Aids Unit Hospital Nacional de Parapléjicos Toledo Spain Rehabilitation department Hospital Nacional de Parapléjicos Toledo Spain

Background: The Box and Block clinical test is a validated and standardized scale for use in the clinical environment that allows the assessment of rough manipulative dexterity. Proposing virtual methods to carry out these assessments is an attempt to eliminate some of the subjectivity that the test may entail depending on the observer and the way in which the patient gives instructions. Applied to the assessment of skills after neurological pathologies, previous experiences in stroke patients have been found. So, this work was centered on the Spinal Cord Injury. Objective: To present the virtual application of the Box and Block scale, as well as details about its design and development for its manipulation based on Leap Motion Controller. Methodology: The relationship between the results obtained in the actual test and in the virtual application in healthy subjects and, mostly, patients with cervical spinal cord injury is analyzed, obtaining a high correlation index between both tests’ performance. Results: A high correlation index was obtained between both tests performance, the real and virtual version of the Box and Block Test. Conclusion: This virtual test can serve as an element to evaluate in the future the effectiveness of the RehabHand prototype based on virtual reality applications with a therapeutic and a rehabilitative sense that, manipulated from Leap Motion Controller, allow the improvement of the manipulative dexterity in patients with neurological diseases such as spinal cord injury.

关键词： Box and Block Test Leap Motion Controller Manual dexterity Spinal Cord Injury Virtual Reality

Growth Traits and the Trade-Offs for Tree Species with Arbuscular Mycorrhizal Fungi in a Tropical Rain Forest Edge at los Tuxtlas,Mexico

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Open Journal of Forestry 2015年第2期5卷 181-194页

作者： Juan Carlos Pena-Becerril Javier Alvarez-Sanchez Guadalupe Barajas-guzmán ana María Quiroz-Ayala departamento de Ecología y Recursos naturales Facultad de CienciasUniversidad Nacional Autónoma de MéxicoCiudad UniversitariaMexico CityMéxico

The effect of arbuscular mycorrhizal fungi on seedling growth across the rain forest-pasture edge has not received much attention. In a tropical rain forest in eastern Mexico, the seedlings of light demanding (Ficus insipida), nonsecondary light demanding (Lonchocarpus cruentus) and shade tolerant species (nectandra ambigens, Coccoloba hondurensis) were grown and transplanted to a forest edge with three inoculation treatments (AM fungus spores and colonized roots, spores, and no inoculum). For all species, stem height, stem diameter, total dry weight, leaf area and net assimilation rate were higher in the pasture. Stem height, stem diameter and root/shoot were higher for L. cruentus, and leaf area ratio, specific leaf area and net assimilation rate were higher for F. insipida;the lowest values of almost all variables were recorded for n. ambigens. L. cruentus and C. hondurensis with mycorrhizae had the highest values for root/shoot and net assimilation rate, respectively. The lowest values of root/shoot and net assimilation rate were observed for nonlight-demanding species in the forest. There were clear trade-offs for the pioneer species between survival and growth, and in underground biomass allocation and assimilation for nonsecondary light demanding, but there was not for the shade-tolerant species.

关键词： Arbuscular Mycorrhizal Fungi Edge Forest Tropical Trees Trade-Offs Tropical Rain Forest

Morphometric and Biochemical Changes in Agave americana *** Induced By Ethyl Methanesulfonate

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Phyton-International Journal of Experimental Botany 2019年第3期88卷 277-284页

作者： S.J.reyes-Zambrano M.L.Ramírez-Merchant C.Arias-Castro M.A.Rodríguez-Mendiola C.A.Lecona-guzmán V.M.Ruíz-Valdiviezo D.González-Mendoza F.A.Gutiérrez-Miceli Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de México.Carretera Panamericana Km.1080TeránTuxtla GutiérrezChiapasC.P.29050México Laboratorios de Análisis Instrumental Bioquímico DEPI del Instituto Tecnológico de TlajomulcoTecnológico Nacional de México.Tlajomulco de ZuñigaC.P.45640México Instituto de Ciencias Agrícolas Universidad Autónoma de Baja California(ICA-UABC)Carretera a Delta s/n Ejido Nuevo LeónCP.21705Baja CaliforniaMéxico

*** *** a crop with very little genetic *** order to evaluate the effect of ethyl methanesulfonate(EMS)to induce variability in in vitro plantlets of ***,different explants(meristems,leaves and roots)were evaluated for the production of *** medium supplemented with ana(2.68μM)and BAP(2.68μM)was *** obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D(0.11μM)and BAP(44μM).The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained,with 60%of the plantlets presenting differences such as dwarfism and different leaf forms,without the presence of spines,as well as an increase in fructan content of 30%with respect to the control *** was increased and this activity is related with higher anthocyanins concentration in *** ***.

关键词： Fructans callus mutagenesis flavonoids anthocyanins phenylalanine ammonia lyase activity

Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer

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Cancer Biology & Medicine 2020年第2期17卷 444-457页

作者： Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria ana Belen Enguita Maria Cebollero Amparo Benito Isabel Alemany Carolina del Castillo Ricardo Ramos ana Ramirez de Molina Enrique Casado Maria Sereno ana Ramirez de Molina1 Enrique Casado5Maria Sereno5 XIMDEA Food InstituteCEI UAM+CSICMadrid 28702Spain 12 de Octubre University Hospital Madrid 28041Spain Gregorio Maranon University Hospital Madrid 28009Spain Ramon y Cajal University Hospital Madrid 28034Spain Infanta Sofia University Hospital San Sebastian De Los ReyesMadrid 28702Spain Fundacion Alcorcon University Hospital AlcorconMadrid 28922Spain Prlncipe de Asturias University Hospital Alcala De HenaresMadrid 28805Spain La Paz University Hospital Madrid 28046Spain University Hospital of Torrejon Torrejon De ArdozMadrid 28850Spain Clinico San Carlos University Hospital Madrid 28040Spain Parque Cientifico de Madrid Foundation Madrid 28049Spain

Objective:Long-term survivors(LS)of non-small cell lung cancer(nSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with *** are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the *** this multi-center study,we focused on LS of advanced nSCLC with OS above 36 months to perform a clinical-pathological and molecular ***:In the first step,we conducted a clinical-pathological characterization of the ***,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RnA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the ***:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type nSCLC with an ECOG of *** obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)***,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(AnCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of nSCLCLS with no driver alterations,obtaining a differential RnA-seq/RT-PCR profile showing an abnormal expression of SF genes.

关键词： Long-term survivors non-small cell lung cancer surfactant proteins

dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez ana Serna-Gallego Esperanza Muñoz-Muela María de los reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan delgado Antonio Domínguez-Petit Alejandro deniz Reginal Dusseck-Brutus ana Escoresca-Ortega Fátima Espinosa nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores navarro-Amuedo Dolores nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain neurology and Clinical neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFn-α).In COVID-19 there is a deficit in DC numbers and IFn-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 dendritic cell Long-COVID

Species diversity of Basidiomycota

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Fungal Diversity 2022年第3期114卷 281-325页

作者： Mao-Qiang He Rui-Lin Zhao Dong-Mei Liu Teodor T.denchev Dominik Begerow Andrey Yurkov Martin Kemler ana M.Millanes Mats Wedin A.R.McTaggart Roger G.Shivas Bart Buyck Jie Chen Alfredo Vizzini Viktor Papp Ivan V.Zmitrovich naveed Davoodian Kevin D.Hyde State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101People’s Republic of China College of Life Sciences University of Chinese Academy of SciencesHuairou DistrictBeijing 100408People’s Republic of China Institute of Ecology Chinese Reserach Academy of Environmental SciencesBeijing 100012People’s Republic of China Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences2 Gagarin St.1113 SofiaBulgaria Evolution der Pflanzen und Pilze Ruhr-Universität BochumND 03Universitätsstr.15044801 BochumGermany Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Inhoffenstraße 7B38124 BraunschweigGermany departamento de Biología y Geología Física y Química InorgánicaUniversidad Rey Juan Carlos28933 MóstolesSpain department of Botany Swedish Museum of Natural HistoryP.O.Box 5000710405 StockholmSweden Queensland Alliance for Agriculture and Food Innovation The University of QueenslandBrisbane 4001Australia Centre for Crop Health University of Southern QueenslandToowoomba 4350Australia Institut pour Systematique EcologieBiodiversite(ISYEBUMR 7205)Sorbonne universitéMuséum National d’Histoire Naturelle CP 39CNRSDépartement Origines et Evolution12 Rue Buffon75005 ParisFrance Facultad de Ciencias Biológicas y Agropecuarias PeñuelaUniversidad VeracruzanaAmatlán de los Reyes94945 VeracruzMéxico Dipartimento di Scienze della Vita e Biologia dei Sistemi Universitàdi TorinoViale P.A.Mattioli 2510125 TorinoItaly department of Botany Hungarian University of Agriculture and Life Sciences1518 BudapestHungary Komarov Botanical Institute Russian Academy of SciencesProfessor Popov st.2Saint PetersburgRussia 197376 Royal Botanic Gardens Victoria Birdwood AvenueMelbourneVIC 3004Australia Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of Science

Fungi are eukaryotes that play essential roles in *** fungi,Basidiomycota is one of the major phyla with more than 40,000 described *** review species diversity of Basidiomycota from five groups with different lifestyles or habitats:saprobic in grass/forest litter,wood-decaying,yeast-like,ectomycorrhizal,and plant *** studies of Agaricus,Cantharellus,Ganoderma,Gyroporus,Russula,Tricholoma,and groups of lichenicolous yeast-like fungi,rust fungi,and smut fungi are used to determine trends in discovery of *** each case study,the number of new species published during 2009–2020 is analysed to determine the rate of *** rates differ between taxa and reflect different states of progress for species discovery in different *** results showed that lichenicolous yeast-like taxa had the highest publication rate for new species in the past two decades,and it is likely this trend will continue in the next *** species discovery rate of plant parasitic basidiomycetes was low in the past ten years,and remained constant in the past 50 *** also found that the establishment of comprehensive and robust taxonomic systems based on a joint global initiative by mycologists could promote and standardize the recognition of *** estimated that more than 54,000 species of Basidiomycota will be discovered by 2030,and estimate a total of 1.4–4.2 million species of Basidiomycota *** numbers illustrate a huge gap between the described and yet unknown diversity in Basidiomycota.

关键词： Biodiversity Fungi Species number Taxonomy