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Journal of Renewable Materials 2019年第2期7卷 117-128页

作者： Larissa Stieven Montagna Isabela CÉSAR Oyama Rita de CÁSSIA Barbosa Camargo Lamparelli ana paula silva THAIS Larissa do Amaral Montanheiro ana paula Lemes Federal University of São Paulo(UNIFESP) Institute of Science and TechnologyTechnology Laboratory of Polymers and Biopolymers(TecPBio)Talim330São Josédos CamposSP12231-280BrazilSão Josédos CamposSPBrazil

Biodegradable polymers have been increasingly used for scientific and commercial applications because they are similar to some conventional thermoplastics and exhibit the ability of ***(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)nanocomposites films with 1 and 2 wt% of carbon nanotubes(CNT)were prepared by solution mixing,followed by solvent *** this work,PHBV/CNT nanocomposites were submitted to biodegradation in an aqueous medium for 34 days through a respirometric ***,the PHBV films were analyzed by the CO2 production and mineralization as a response of a microbial attack,which was monitored by back titration during all the *** films were also characterized by measuring the weight loss;crystallinity was evaluated by differential scanning calorimetry(DSC)and the surface morphology by scanning electron microscopy(SEM).By analyzing the weight loss of the films,it was observed that adding CNT increases the resistance to biodegradation *** obtained values of CO2 production and mineralization of the samples,as well as the values of weight loss,showed that the biodegradation of PHBV/CNT nanocomposites was minor in comparison to neat *** addition of CNT in PHBV matrix influences the surface morphology,causing the presence of cavities and an increase of roughness.

关键词： Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) carbon nanotube nanocomposites biodegradation in aqueous medium

Integrating species and successional classes for wood production in a mixed forest restoration system in a neotropical region

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Journal of Forestry Research 2021年第6期32卷 2313-2321页

作者： ananias Francisco Dias Junior ana Lucia Martins Sodero Pincelli ana paula Camara da silva Alison Moreira da silva Natalia Dias de Souza Mario Tommasiello Filho Jose Otavio Brito Wood and Forestry Science Department Federal University of EspIrito Santo(UFES)Av.Governador Lindemberg316JerOnimo Monteiro CityES 29550-000Brazil São Paulo State School of Technology(FATEC/SP) Av.DiacOno Jair de Oliveira651PiracicabaSP 13414-141Brazil Forest Products Department Federal Rural University of Rio de Janeiro(UFRRJ)BR 465km 07SeropEdicaRJ 29890-000Brazil University of SAo Paulo “Luiz de Queiroz”College of Agriculture(USP/ESALQ)Av.PAdua Dias11PiracicabaSP 13418-900Brazil

As forests in neotropical regions,particularly in developing countries,are devastated,interventions to restore biodiversity and its ecological functions are *** producers have thus been encouraged to grow trees for wood production as an economic *** objective of this study was to quantify the increment of wood density of four forest species from different successional classes of a mixed system of restoration in a neotropical forest in *** discs were sampled at breast height and analyzed radially by X-ray densitometry to obtain apparent density and basic *** trees each of a species from the pioneer,early and late secondary successional stages and of the dominant species in the climax *** radial profiles indicated increasing density from the pith to bark of trunks,except for some variations due to wood defects and *** density was 0.576 g cm^(-3),0.655 g cm^(-3),0.706 g cm^(-3)and 0.775 g cm^(-3),respectively,for Peltophorum dubium,Schinus terebinthifolius,Cariniana estrellensis and Hymenaea *** profiles indicated higher amplitudes in the apparent densities for slow-growing species.X-ray densitometry generated parameters such as minimum,average and maximum densities,and radial density *** parameters are important for understanding the ecological functional role of successional classes of the Atlantic Forest from the Neotropical region.

关键词： X-ray densitometry Timber species of neotropical forest Successional classes of forest species Maintenance of forest restoration

Ecophysiological acclimatization to cyclic water stress in Eucalyptus

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Journal of Forestry Research 2020年第3期31卷 797-806页

作者： Rogerio de Souza Noia Junior Genilda Canuto Amaral Jose Eduardo Macedo Pezzopane Mariana Duarte silva Fonseca ana paula Camara da silva Talita Miranda Teixeira Xavier Department of Biosystems Engineering ESALQ University of Sao PauloPiracicabaSao PauloBrazil Department of Forestry Sciences NEDTEC Federal University of Espirito SantoJero nimo MonteiroEspirito SantoBrazil

Drought is considered the main environmental factor limiting productivity in eucalyptus plantations in Brazil. However, recent studies have reported that exposure to water deficit conditions enables plants to respond to subsequent stresses. Thus, this study investigates the ecophysiological acclimatization of eucalyptus clones submitted to recurrent water deficit cycles. Eucalyptus seedlings were submitted to three recurrent water deficit cycles and anatomical, morphological and physiological changes were analyzed. The results were:(1) Eucalyptus seedlings responded to water deficits by directing carbohydrates to root and stem growth;(2) Size and number of stomata were reduced;(3) Stomatal conductance decreased which allowed the plants to reduce water losses through transpiration,increasing instantaneous water use efficiency;(4) The relationship between gas exchanges and available water contents allowed the seedlings to uptake the retained soil water athigher tensions;and,(5) Physiological recovery from subsequent water deficits became faster. As a result of these changes, the eucalyptus seedlings recovered from the same degree of water stress more rapidly.

关键词： Carbon partition Drought Gas exchange Morpho-physiological changes Photosynthetic apparatus

P53 protein expression and cell viability in irradiated peripheral blood mononuclear cells as bioindicators of radiosensitivity

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Journal of Biophysical Chemistry 2011年第2期2卷 63-67页

作者： Mariana Brayner Cavalcanti ana paula Galvao da silva Rafael de Freitas e silva Ademir Amaral Grupo de Estudos em Radioprotecao e Radioecologia(GERAR) Laboratorio de Modelagem e Biodosimetria Aplicada(LAMBDA)Departamento de Energia NuclearUniversidade Federal de PernambucoCidade UniversitariaPernambucoBrazil anaptysbio Incorporation Cell BiologySan DiegoUSA

Cellular radiosensitivity is directly correlated with the mechanism of DNA repair, in which p53 protein plays a major role. In this context, this study correlated cell death with p53 expression in lymphocytes irradiated in vitro with different doses of gammaradiation. For this, peripheral blood samples were collected from 10 healthy subjects. Each sample was divided in aliquots and, separately, irradiated with doses of 0,5;2 and 4 Gy. After this, peripheral blood mononuclear cells (PBMCs) were isolated and cultivated during 72 hours in 5% CO2 at 37oC without mitogen stimulation. The expression of p53 protein was evaluated by flow cytometry. In parallel, cell viability was determined by trypan blue staining. Statistical analysis was performed us-ing analysis of variance (ANOVA), differences were considered as statistically significant when p < 0.05. The results showed an increase of p53 expression with the absorbed dose, which was proportional to cell death, suggesting that p53 can be used as bioindicator of individual radiosensitivity.

关键词： P53 Protein Radiosensitivity Lymphocytes Flow Cytometry Cell Viability

纳特竖蟾(无尾目:滑背蟾科)的细胞遗传、与相关属物种的核型相似性并兼论其分类地位(英文)

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动物学报 2007年第2期53卷 285-293页

作者： Fernando anaNIAS André Luis BOMBEIRO Catia Donizete Brunaikovics da silva ana paula Zampieri silva Célio Fernando Baptista HADDAD Sanae KASAHARA Curso de Ciências Biológicas Universidade S■o Francisco (USF) Av. S■o Francisco de Assis 218-Jardim S■o José 12916-900 Bragana Paulista SP Brasil Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Rio Claro SP Brasil Curso de Ciências Biológicas Universidade Braz Cubas (UBC) Mogi das CruzesSPBrasil Departamento de Zoologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Rio Claro SP Brasil

本文报道纳特竖蟾(Eupemphix nattereri Steindachner,1863)巴西3个产地标本的核型,均为2n=22,由1对端部和10对中或亚中部着丝点染色体组成。仅一对NORs位于No.11染色体对长臂端部,而其位置有别于常见的具有标志性意义的近着丝点位置。... 详细信息

本文报道纳特竖蟾(Eupemphix nattereri Steindachner,1863)巴西3个产地标本的核型,均为2n=22,由1对端部和10对中或亚中部着丝点染色体组成。仅一对NORs位于No.11染色体对长臂端部,而其位置有别于常见的具有标志性意义的近着丝点位置。该NORs的位置为rDNA为探针的荧光原位杂交(FISH)所确认。各染色体对中,着丝点C-带明显,插入或端带偶见。某些标本的No.8同源染色体对间C-带的大小随异染色质化的程度不同而变化。居于较小的实验标本量,这种在3个产地的雌或雄性标本之一中观察到的C-带异型现象可能为种下细胞地理学变异,亦或为细胞学意义的性染体分化。3产地之一的标本核型的No.11着丝点C-带异染色质化的程度较高。CMA3染色检测到部分GC-rich区域,DAPI染色未显示任何AT-rich区。成功获得BrdU复制带,并将其与滑背蟾类动物(leiuperid)中近缘属及物种进行对比分析。比较结果表明,纳特竖蟾的核型与斑符泡蟾种组(Physalaemus signifergroup)难以相互区别,而与肿肋蟾属(Pleurodema Tschudi,1838)极为相似。核型数据支持形态学上将纳特竖蟾、二光肿肋蟾[Pleurodema diplolister(Peters,1870)]和短头肿肋蟾[***(Cope,1869)

关键词：纳特竖蟾染色体 Ag-NOR C-带荧光染色荧光原位杂交

A Promising Strategy for the Treatment of Onychomycosis with Curcumin and Photodynamic Therapy

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Journal of Pharmacy and Pharmacology 2015年第9期3卷 434-437页

作者： ana paula da silva Fernanda M. Carbinatto Vanderlei S. Bagnato Natalia M. Inada University of Sao Paulo Physics Institute of Sao Carlos 13566-590 Sao Carlos SP Brazil

Onychomycosis is a disease of high incidence in the nail plate and responsible for approximately half of the cases of nail infections. Conventionally, dermatologists prescribe antibiotics and antifungals for long periods for its treatment. The high incidence of this type of infection and the increase of microbial strains resistant to the available drugs have justified the importance of the development of new technologies and treatments. This paper presents the photodynamic therapy as an alternative treatment of onychomycosis. New strategies for the use of curcumin as a photosensitizer and its therapeutic response were investigated in different formulations （gel and emulsion）. Photodynamic therapy is a promising technique by which microorganisms are eliminated by a photosensitizing compound, light and oxygen. It was evaluated in two patients who had developed lesions in the fmgernails caused by onychomycosis for approximately 10 years. The lesions were treated by photodynamic therapy with curcumin. The therapeutic efficacy was observed after a maximum of six photodynamic therapy sessions without any other adjuvant therapy. Curcumin has become more promising than the therapeutic standard as it is a natural and versatible drug for incorporation in different formulations, at low cost and low probability of side effects.

关键词： Onychomycosis photodynamic therapy curcumin formulations.

Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans

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Neural Regeneration Research 2025年第2期20卷 343-353页

作者： ana Milena silva Olaya Fernanda Martins Almeida ana Maria Blanco Martinez Suelen Adriani Marques PhD Program in Pathological anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil Biomedical Science Institute Federal University of Rio de JaneiroRio de JaneiroBrazil Graduate Program in Pathological anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil Graduate Program in Pathological anatomy(PPGAP/UFRJ) Department of Neurobiology/Institute of BiologyCampus do GragoatáNiteróiRio de JaneiroBrazil

Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical ***,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord *** of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional *** the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in *** article is a literature review from databases(PubMed,Science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were ***,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.

关键词： bioengineering biomaterials cell therapy humans non-human primates spinal cord injury stem cell therapy

Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity:a review

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Journal of Integrative Medicine 2018年第4期16卷 211-222页

作者： Bruno Jose Martins Da silva Amanda anastacia Pinto Hage Edilene Oliveira silva ana paula Drummond Rodrigues Laboratory of Parasitology and Laboratory of Structural Biology Institute of Biological SdencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Structural Biology and Bioimaging Rio de JaneiroRio de Janeiro 21941-901Brazil Laboratory of Electron Microscopy Department of Health SurveillanceMinistry of HealthEvandro Chagas InstituteBelemPard 66087-082Brazil

Leishmaniasis, a neglected disease caused by Leishmania protozoans, primarily affects people in tropical and subtropical areas. Chemotherapy based on the use of pentavalent antimonials, amphotericin B, paromomycin, miltefosine and liposomal amphotericin B is currently the only effective treatment. However, adverse effects, long-term treatment and the emergence of parasite resistance have led to the search for alternative treatments. Natural products used in traditional medicine provide an unlimited source of molecules for the identification of new drugs, and the Amazon region has abundant biodiversity that includes several species of plants and animals, providing a rich source of new products and com- pounds. Although the literature describes numerous promising compounds and extracts for combating Leishmania protozoans, the results of such research have not been embraced by the pharmaceutical industry for the development of new drugs. Therefore, this review focused on the antileishmanial activity of extracts, isolated compounds and essential oils commonly used by the local population in the Brazilian Amazonian region to treat several illnesses and described in the literature as promising compounds for combating leishmaniasis.

关键词： Amazonian plants Leishmaniasis treatment Plant compounds Plant extracts Plant oils

In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum

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Journal of Integrative Medicine 2018年第6期16卷 404-410页

作者： Bruno Jose Martins Da silva Sandro Wilson Gomes Pereira ana paula Drummond Rodrigues Jose Luiz Martins Do Nascimento Edilene Oliveira silva Laboratory of Structural Biology Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Structural Biology and Bioimaging(INCT-INBEB) Rio de Janeiro 21941-901Brazil Laboratory of Electron Microscopy Department of Health SurveillanceMinistry of HealthEvandro Chagas InstituteBelemPard 66087-082Brazil Laboratory of Molecular and Cellular Neurochemistry Institute of Biological SciencesFederal University of PardBelemPard 66075-110Brazil National Institute of Science and Technology in Neuroimmunomodulation(INCT-NIM) Rio de Janeiro 21040-360Brazil

Objective： In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root （AEPa） on Leishmania infanturn proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. Methods： I., infantum promastigotes were treated with 50 and 100 μg/mL AEPa for 72 h and then antipro- mastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. Results： Treatment with AEPa reduced the numbers ofL infantum promastigotes （50% inhibitory concen- tration （ICso） = 65.9μg/mL; selectivity index （SI）= 22.1） and amastigotes （ICso = 37.9 μg/mL; SI = 38.5） compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment （IC50 = 0.2μg/mL）. AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa （100 μg/mL） promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells （50% cytotoxic concentration 〉1000μg/mL）. Conclusion： AEPa presented antileishmanial activity against the promastigotes and amastigotes of I.. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

关键词： Leishmanicidal activity Leishmania infantum Reactive oxygen species Apoptosis Physalis angulata

analysis of the Most Likely Regions of Stability of an NCS and Design of the Corresponding Event-driven Controller

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International Journal of Automation and computing 2018年第1期15卷 39-51页

作者： ana paula Batista Fabio Goncalves Jota Centre for Intelligent Energy Research Federal Centre of Technological Education of Minas Gerais Belo Horizonte Brazil Graduate Program in Electrical Engineering Federal University of Minas Gerais Belo Horizonte Brazil

In this paper, some issues related to design and analysis of real networked control systems （NCS） under the focus of the most likely region of stability are addressed. Such a system is cumbersome due to its inherent variable time delays, ranging from microseconds to hours. To show the influence of such huge variations in the control performance, a laboratory-scale luminosity system has been setup using the Internet as part of the control loop with dominant time constant in the order of milliseconds. Proportional and integral （PI） control strategies with and without explicit compensation for the time-delay variations were implemented using an event-driven controller. Using the well-known Monte Carlo method and subsequent analyses of time responses, it has been possible to identify the most likely region of stability. Some experimental results show the influence of the statistical parameters of the delays on the determination of the most likely regions of stability of the NCS and how these can be used in assessment and redesign of the control system. The experiments show that much larger delays than one sample period can be supported by real NCSs without becoming unstable.

关键词： Networked control systems real NCS delay compensation most likely regions of stability delay statistics.