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Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco

Asian Pacific Journal of Tropical Medicine 2019年第1期12卷 32-37页

作者： Messaoudi Mouhcine Laglaoui Amin amzazi saaid Hammani Khalil Benbacer Laila El Mzibri Mohammed Unité de Biologie et Recherche Médicale CNESTEN Laboratoire de Biochimie-Immunologie Faculté des Sciences Mohammed V University équipe de Recherche en Biotechnologies & Génie des Biomolécules Faculté des Sciences et Techniques de Tanger Abdelmalek Essaadi University Laboratoire de Ressources Naturelles et Environnement Faculté Polydisciplinaire de Taza Sidi Mohammed Ben Abdellah University

Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander(N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bioassay on two human cancer cell lines, namely human colon adenocarcinoma cell line HT-29 and human breast cancer cell line MDA-MB-231. The antioxidant property of N. oleander extracts was assessed by DPPH scavenging and he agar disc diffusion method was used for the determination of an毬-carotene bleaching tests. Ttimicrobial activity against different strains. Results: Using cell viability indices, the WST-1 test revealed that both extracts reduced cell viability in both cell lines. Our results also showed that aqueous extract was more active than ethanolic extract, with IC50 values of(1.67±0.22) μg/mL and(2.36±0.44) μg/mL on MDA-MB-231 cells, and(2.89±0.35) μg/mL and(5.09±0.52) μg/mL on HT29 cells, respectively. The study of the antioxidant activity showed that N. oleander extracts had a considerable scavenging capacity and exerted a significant preventive effect against the oxidation of 毬-carotene by the peroxide radicals. However, the antibacterial test showed that both ethanolic and aqueous extracts of N. oleander had a moderate antibacterial effect limited only to Gram-positive bacteria. Conclusions: Our results shows that N. oleander aqueous and ethanolic extracts have significant cytotoxic activities against tumor cell lines and possesses a strong antioxidant capacity, suggesting the presence of active compounds in N. oleander leaves that could be a potential source of phytochemicals with high medicinal value to be used in cancer treatment and prevention.

关键词： Nerium oleander Anti-cancer Cytotoxicity Antioxidant Antimicrobial Bioactive compounds

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Serological Detection of Tomato Pepino mosaic virus in Morocco

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Journal of Agricultural Science and Technology(B) 2013年第12期3卷 847-852页

作者： Amal Souiri saaid amzazi Hayat Laatiris Moulay Mustapha Ennaji Mustapha Zemzami Laboratory of Virology Microbiology and Quality/Ecotoxicology and Btlodiverity (L VMQ/ETB) Faculty of Science and Technics Mohammedia (FSTM) University Hassan II Mohammedia Casablanca (UH2MC) Mohammedia 20650 Morocco Laboratory of Biochemistry and Immunology Faculty of Sciences Agdal University of Mohammed V Rabat 10080 Morocco Laboratory of Sanitary Control Control Unit of Plants Domaines Agricoles Madmora Sald 11000 Morocco

Pepino mosaic virus (PepMV), monopartite RNA virus, 6,500 pb, belonging to Flexiviridae and Potexvirus group, is highly infectious and easily transmissible. Its economic impact is major for the tomato producer"s countries. Prevention, based on early virus detection is the only effective control measure. Monoclonal antibodies appeared to be very useful tool. The authors used for the production of monoclonal antibodies hybridomas technique, by fusing spleen cells of immunized BALB/c mice to PepMV and SP2/O cancerous cells. The aim of this work is to produce hybridomas producers of Mab that could be used for ELISA in Morocco. At the same time, these efforts will serve to decrease expenses of producers concerning phytosanitory control. We obtained 16 hybridomas lines producers of Mab specific for PepMV. They were tested for efficiencies in ELISA and two lines were retained for production of Mab on large scale (1B11-G10 and 5A1-G5). Isotyping of these two lines showed that they are belonging to IgG1 class and easily purified by affinity chromatography in agarose column by protein A. The conjugation of these two antibodies to alkaline phosphatase has been verified by DAS-ELISA. These antibodies will enable to diagnose the disease from infected tomato plants, integrating several serological tests to control it and target the actions of struggles.

关键词：番茄花叶病毒摩洛哥血清学检测 ELISA检测 DAS-ELISA 单克隆抗体杂交瘤技术马铃薯X病毒

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