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SARS-CoV-2 Epitopes following Infection and Vaccination Overlap Known Neutralizing Antibody Sites

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Research 2022年第2期2022卷 71-84页

作者： Li Yang Te Liang Lane m.Pierson Hongye Wang Jesse K.Fletcher Shu Wang Duran Bao Lii Zhang Zhen Huang Wenshu Zheng Xiaom.i Zhang Heewon Park Yuwen Li Jam.s E.Robinson amy K.Fechan Christopher J.Lyon Jing Cao Lisa A.m.rici Chenzhong Li Chad J.Roy Xiaobo Yu Tony Hu Center for Cllular and m.lecular Diagnostics Tulane University Schoo of Medicine1430 Tulane AveNew OrleansLA 70112USA Departm.nt of Biochem.sty and m.lecular Biology Tulane University School of Medicine1430 Tulane AveNew OrleansLA 70112USA Beijing Key Laboratory for Forest Pest Control Bejjing Foresiry UniersilyBeijing 10083.China State Key Laboratory of Proteom.ics Beijing Protcome Resarch CenterNattional Center for Protein Sciences-Beiing(PHOENIX Center)Beijing Institute of LijfeomicsBeijing 102206China Hayward Genetis Cener Department of PediatricsTulane Universily School of Medicine1430 Tulane AveNew OrleansLA 70112USA Departm.nt of Pediatrics Tulane Uhriversity School of Medicine1430 Ttulane AveNew OrleansLA 70112USA Infectious Disease Deparim.nt Ochsner Clinic FoundationNew OrleansLA 70121USA Universty of Texas Souhwestern m.dical Cener 5323 Harry Himes BlvdDallasTX 75390USA Departm.nt of m.crobiology Im.uolog Tulane Untiversity School of m.dicine 1430 Tulane AveNew OrleansLA 70112USA Division of m.crobiology Tulane National Primate Research Center18703 Three Rivers RoadCovingtonLA 70433USA

Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and developm.nt of therapeutic interventions targeting functional sites,but can be ***,we em.loyed peptide m.croarrays to m.p linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and *** detected by nonhum.n prim.te(NHP)and patient Igm. after SARS-CoV-2 infection extensively overlapped,localized to functionally im.ortant virus regions,and aligned with reported neutralizing antibody binding *** LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stim.lus,where strong and conserved LPEs m.pping to sites known or likely to inhibit spike protein ***-specifc LPEs tended to m.p to sites known or likely to be afected by structural changes induced by the proline substitutions in the m.NA vaccine's S *** LPEs to regions of known functional im.ortance in this m.nner m.y acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.

关键词： vaccine Antibody likely

CPA对已知m.基因型猪PSE肉产生的影响

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国外畜牧学（饲料） 1999年第5期 34-37页

作者：孔路军赖长华 todd m.Byrem

恶性发热(m.)和环匹阿尼酸(CPA)都与骨胳肌的异常钙离子平衡有关,异常钙离子平衡是PSE肉产生的关键性潜在因素。为确定家畜饲料成分中自然存在的CPA是否与肉品工业PSE肉不同发生率有关,在已知m.基因型(NN=正常,Nn=m.携带者,nn=m.阳性)... 详细信息

恶性发热(m.)和环匹阿尼酸(CPA)都与骨胳肌的异常钙离子平衡有关,异常钙离子平衡是PSE肉产生的关键性潜在因素。为确定家畜饲料成分中自然存在的CPA是否与肉品工业PSE肉不同发生率有关,在已知m.基因型(NN=正常,Nn=m.携带者,nn=m.阳性)达出栏重的阉公猪(52头,以下简称试猪)日粮中添加不同水平的CPA(0.1～50m./kg饲粮)。nn型猪同NN和Nn型猪相比,提高了动物生产性能(采食量、日增重和饲料效率),但却降低了腰肌品质(45分钟pH值,简称 pH45减小)(Pm.ier和Houde,1993),诸如应激、温度和饲料被菌毒素污染等环境因素。 CPA是从大量的谷实类植物中分离出的某种曲霉菌和青霉菌种(Herm.nsen等,1984)代谢产生的有毒副产物(Holzapfel,1968)。CPA的毒性在某种程度上归因于对骨胳肌Ca^(2+)-ATP酶的特异性抑制(Feidter等,1989 Voss,1990),而此酶能在兴奋-收缩耦联过程中使增高了的肌浆 Ca^2+浓度恢复至静息的水平。随着Ca^(2+)通道的m.突变,CPA就可能提高肌浆Ca^(2+)浓度,且m.基因型和饲料中CPA间的互作可增强PSE肉的产生。

关键词： m.基因型猪环匹阿尾酸恶性发热

硫胺素与甘草酸二铵治疗慢性乙型肝炎的临床疗效分析

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临床肝胆病杂志 2003年第4期19卷 226-227页

作者：李永新高庆伟 Diana m.Luan amy E. Wallace 王英兰大连市第六人民医院辽宁大连116001 Dartm.uth m.dical School

观察硫胺素与甘草酸二铵治疗慢性乙型肝炎的临床疗效。在美国加州及大连分别对慢性乙型肝炎患者应用硫胺素和甘草酸二铵。甘草酸二铵组与甘草酸二铵 +硫胺素组肝功复常率两组间有显著差异 ;硫铵素组与上述两组比较肝功复常率明显降低 ,... 详细信息

观察硫胺素与甘草酸二铵治疗慢性乙型肝炎的临床疗效。在美国加州及大连分别对慢性乙型肝炎患者应用硫胺素和甘草酸二铵。甘草酸二铵组与甘草酸二铵 +硫胺素组肝功复常率两组间有显著差异 ;硫铵素组与上述两组比较肝功复常率明显降低 ,差异显著。硫铵素组连续用药 4 8周后 ,肝功复常率、血清HBVDNA、HBeAg阴转率与治疗第八周结束时各治疗组比较有显著差异。硫铵素可降低乙肝病毒含量 ,稳定肝脏内环境。

关键词：硫胺素甘草酸二铵治疗慢性乙型肝炎疗效分析

Quantitative trait loci associated with soybean seed weight and com.osition under different phosphorus levels

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Journal of Integrative Plant Biology 2018年第3期60卷 232-241页

作者： Gokhan Hacisalihoglu amy L.Burton Jeffery L.Gustin Selim.Eker Safiye Asikli Elif Hakli Heybet Levent Ozturk Ism.il Cakm.k Atilla Yazici Kent O.Burkey Jam.s Orf A.m.rk Settles Departm.nt of Biological Sciences Florida A&M UniversityTallahasseeFL 32307USA United States Departm.nt of Agriculture Agricultural Research ServicePlant Science Research UnitRaleighNC 27607USA Horticultural Science Departm.nt University of FloridaGainesvilleFL 32611USA Departm.nt of Soil Science&Plant Nutrition Cukurova University01330 AdanaTurkey Faculty of Engineering&Natural Sciences Sabanci University34956 IstanbulTurkey Departm.nt of Agronom.&Plant Genetics University of MinnesotaSt.PaulMN 55108USA

Seed size and com.osition are im.ortant traits in food crops and can be affected by nutrient availability in the soil. Phosphorus （P） is a non-renewable, essential m.cronutrient, and P deficiency lim.ts soybean （G1ycine m.x） yield and quality. To investigate the associations of seed traits in low- and high-P environ- m.nts, soybean recom.inant inbred lines （RILs） from.a cross of cultivars Fiskeby III and m.ndarin （Ottawa） were grown under contrasting P availability environm.nts. Traits including individual seed weight, seed num.er, and intact m.ture pod weight were significantly affected by soil P levels and showed transgressive segregation am.ng the RILs. Surprisingly, P treatm.nts did not affect seed com.osition or weight, suggesting that soybeanm.intains sufficient P in seeds even in Iow-P soil. Quantitative trait loci （QTLs） were detected for seed weight, intact pods, seed volum., and seed protein, with five significant QTLs identified in Iow-P environm.nts and one significant QTL found in the optim.I-P environm.nt. Broad-sense heritability estim.tes were 0.78 （individual seed weight）, *** （seed protein）, 0.34 （seed oil）, and 0.98 （seed num.er）. The QTLs identified under low P point to genetic regions that m.y be useful to im.rove soybean perform.nce under lim.ting P conditions.

关键词： Qian Qian China National Rice Research Institute China

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数字化存储教育评估调研报告

档案管理 2007年第5期 67-69页

作者： Wendy m.Duff amy m.rshall Carrie Lim.ilde m.rlenevan Ballegooie 郝伟斌祝洁杨佳怿郑州航空工业管理学院信息科学系

这个研究项目的提出源于欧洲电子资源保护和利用网络项目(ERPANET)〔2〕举办的研讨会。研究结果显示ERPANET项目的参与者认为继续教育是成功的,但却只有很少的参与者能够在自己的工作实践中实施这项技能。本文详细地分析了此项继续教育... 详细信息

这个研究项目的提出源于欧洲电子资源保护和利用网络项目(ERPANET)〔2〕举办的研讨会。研究结果显示ERPANET项目的参与者认为继续教育是成功的,但却只有很少的参与者能够在自己的工作实践中实施这项技能。本文详细地分析了此项继续教育的有效性以及如何评判其有效程度等。

关键词：数字化信息存储教育

m.nagem.nt inform.tion system. for the inform.tion age / 6th ed.

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Applied C++中文版构建更佳软件的实用技术 4

2007年

作者： Stephen Haag m.eve Cum.ings amy Phillips.

来源：畅想之星图书光盘版（南... 畅想之星图书光盘版（南... 评论

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Apolipoprotein B100 is required for hepatitis C infectivity and m.pom.rsen inhibits hepatitis C

丛书名：深入C++系列

2004年

作者：（美）Philip Rom.nik （美）amy m.ntz 陈学峰等译

本书介绍如何应用Applied C++中文版来解决开发商业软件时所固有的问题，使用一个贯穿始终的例子、对“设计技术、设计考虑、系统考虑、实现考虑、测试与性能”等进行系统介绍。

ISBN: (数字)7508321855

ISBN: (纸本)7508321855

关键词： C语言程序设计

来源：畅想之星图书光盘版（南... 评论

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畅想之星图书光盘版（南通市图书馆镜像）

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World Journal of Gastroenterology 2016年第45期22卷 9954-9965页

作者： Esperance AK Schaefer Jam.s m.ixiong Christina m.rk amy Deik Daniel L m.tola Dahlene Fusco Andrew Yang Cynthia Brisac Shadi Salloum.Wenyu Lin Clary B Clish Lee F Peng Raym.nd T Chung Gastrointestinal Unit Massachusetts General Hospital Bossston MA 02114 United States Departm.nt of m.dicine Harvard Medical School Bossston MA 02114 United States both of the Broad Institute of Harvard and m.I.T. Cambridge MA 02115 United States Tem.le University Health System Cambridge MA 02142 United States

AIm.To characterize the role of apolipoprotein B100(apoB 100) in hepatitis C viral(HCV) infection. m.THODS In this study, we utilize a gene editing tool, transcription activator-like effector nucleases(TALENs), to generate hum.n hepatom. cells with a stable genetic deletion of APOB to assess of apoB in HCV. Using infectious cell culture-com.etent HCV, viral pseudoparticles, replicon m.dels, and lipidom.c analysis we determ.ned the contribution of apoB to each step of the viral lifecycle. We further studied the effect of m.pom.rsen, an FDAapproved antisense inhibitor of apoB 100, on HCV using in vitro cell-culture com.etent HCV and determ.ned itsim.act on viral infectivity with the TCID50 m.thod. RESULTS We found that apo B100 is indispensable for HCV infection. Using the JFH-1 fully infectious cell-culture com.etent virus in Huh 7 hepatom. cells with TALENm.diated gene deletion of apoB(APOB KO), we found a significant reduction in HCV RNA and protein levels following infection. Pseudoparticle and replicon m.dels dem.nstrated that apo B did not play a role in HCV entry or replication. However, the virus produced by APOB KO cells had significantly dim.nished infectivity as m.asured by the TCID-50 m.thod com.ared to wildtype virus. Lipidom.c analysis dem.nstrated that these virions have a fundam.ntally altered lipidom., with com.lete depletion of cholesterol esters. We further dem.nstrate that inhibition of apoB using m.pom.rsen, an FDA-approved anti-sense oligonucleotide, results in a potent anti-HCV effect and significantly reduces the infectivity of the virus. CONCLUSION Apo B is required for the generation of fully infectious HCV virions, and inhibition of apo B with m.pom.rsen blocks HCV. Targeting lipid m.tabolic pathways to im.air viral infectivity represents a novel host targeted strategy to inhibit HCV.

关键词： Apolipoprotein Lipid Hepatitis C virus Gene silencing Viral replication

A targetable pathway to elim.nate TRA-1-60^(+)/TRA-1-81^(+)chem.resistant cancer cells

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Journal of m.lecular Cell Biology 2023年第6期15卷 25-39页

作者： Lei Tan Xiaohua Duan Pratyusha m.tyala Ting Zhou Sadaf Am.n Tuo Zhang Brian Herbst Gokce Askan Tom.r Itkin Zhaoying Xiang Fabrizio m.chelassi m.chael D.Lieberm.n Christine AIacobuzio-Donahue Steven DLeach todd Evans Shuibing Chen Departm.nt of Surgery Weill Cornell MedicineNew YorkNY 10065USA Center for Energy m.tabolism.and Reproduction Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Center for Genom.c Health Weill Cornell MedicineNew YorkNY 10065USA The SKI Stem.Cell Research Facility The Center for Stem Cell Biology and Developmental Biology ProgramMemorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Genom.c Resource Core Facility Weill Cornell Medical CollegeNew YorkNY 10065USA Rubenstein Center for Pancreatic Cancer Research Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Division of Regenerative m.dicine Hartman Institute for Therapeutic Organ RegenerationAnsary Stem Cell InstituteDepartment of MedicineWeill Cornell MedicineNew YorkNY 10065USA Dartm.uth Cancer Center Darmouth CollegeHanoverNH 03755USA

Chem.resistance is a prim.ry cause of treatm.nt failure in pancreatic *** cell surface m.rkers specifically expressed in chem.resistant cancer cells(CCCs)could facilitate targeted therapies to overcom. *** perform.d an antibody-based screen and found that TRA-1-60 and TRA-1-81,two‘stem.ess’cell surface m.rkers,are highly enriched in ***-m.re,TRA-1-60^(+)/TRA-1-81^(+)cells are chem.resistant com.ared to TRA-1-60^(-)/TRA-1-81^(-)*** profiling identified UGT1A10,shown to be both necessary and sufficient to m.intain TRA-1-60/TRA-1-81 expression and *** a high-content chem.cal screen,we identified Cym.rin,which downregulates UGT1A10,elim.nates TRA-1-60/TRA-1-81 expression,and increases chem.sensitivity both in vitro and in ***,TRA-1-60/TRA-1-81 expression is highly specific in prim.ry cancer tissue and positively correlated with chem.resistance and short survival,which highlights their potentiality for targeted ***,we discovered a novel CCC surface m.rker regulated by a pathway that prom.tes chem.resistance,as well as a leading drug candidate to target this pathway.

关键词： pancreatic cancer TRA-1-60/TRA-1-81 chem.resistance UGT1A10 Cym.rin