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Horticulture Research 2021年第1期8卷 2404-2415页

作者： Greg S.Goralogia Glenn T.Howe amy M.Brunner Emily Helliwell Michael F.Nagle cathleen Ma Haiwei Lu Amanda L.Goddard Anna c.Magnuson amy L.Klocko Steven H.Strauss Department of Forest Ecosystems and Society Oregon State UniversityCorvallisORUSA Department of Forest Resources and Environmental conservation Virginia TechBlacksburgVAUSA Department of Biology University of Colorado Colorado SpringsColorado SpringsCOUSA

The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public and regulatory acceptance of genetically engineered plants,particularly for wind-pollinated trees such as *** report that overexpression of a poplar homolog of the floral repressor SHORT VEGETATIVE PHASE-LIKE(SVL),a homolog of the Arabidopsis MADS-box repressor SHORT VEGETATIVE PHASE(SVP),delayed the onset of flowering several years in three genotypes of field-grown transgenic *** expression of SVL correlated with a delay in flowering onset and lower floral abundance,and did not cause morphologically obvious or statistically significant effects on leaf characteristics,tree form,or stem *** effects on reproductive and vegetative phenology in spring was modest and *** results suggest that use of SVL and related floral repressors can be useful tools to enable a high level of containment for vegetatively propagated short-rotation woody energy or pulp crops.

关键词： field planted crops

Prophylactic Bactericidal Orthopedic Implants – Animal Testing Study

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journal of Biomedical Science and Engineering 2010年第9期3卷 917-926页

作者： Richard A. Wysk Wayne j. Sebastianelli Rohan A. Shirwaiker Gregory M. Bailey charumani charumani Mary Kennett amy Kaucher Rachel Abrahams Thomas A. Fuller Patricia Royer Robert c. Voigt Paul H. cohen 不详

This paper summarizes preliminary rat studies aimed at identifying the effectiveness of using electrically stimulated silver as a bactericidal agent for indwelling residual hardware devices (RHD). A variety of bactericidal indwelling devices were designed, fabricated and surgically inserted into the medullary cavity of live rats. The rats were inoculated with Staphylococcus aureus to try and induce osteomyelitis. A total of 37 surgeries were performed by implanting the rats with both control and potentially bactericidal devices. As surgical procedures and devices were improved, it appeared that the implants produced antibiotic effects in the animals. All of the control animals and all of the animals where the device failed tested positive for Staphylococcus aureus growth. Of the rats with operational bactericidal devices (those that survived the surgery and incubation period), half tested negative for Staphylococcus aureus. The device designs are discussed in this paper along with the test procedures, operating practices and results. A statistical analysis of the results, which shows a very high confidence level in the effectiveness of electrically stimulated silver as a bactericidal agent/antibiotic, is also presented.

关键词： Antibacterial Antimicrobial Bactericidal Silver Ionic Silver Residual Hardware Devices (RHD) Animal Testing

Genetic and environmental control of fruit maturation,dry matter and firmness in apple(Malus×domestica Borkh.)

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Horticulture Research 2014年第1期1卷 98-109页

作者： David chagne Daya Dayatilake Robert Diack Murray Oliver Hilary Ireland amy Watson Susan E Gardiner jason W johnston Robert j Schaffer Stuart Tustin The New Zealand Institute for Plant&Food Research Limited(Plant&Food Research) Palmerston North Research CentrePalmerston NorthNew Zealand Plant&Food Research Hawke’s Bay Research CentreHavelock NorthNew Zealand Plant&Food Research Motueka Research CentreMotuekaNew Zealand Plant&Food Research Mount Albert Research CentreAucklandNew Zealand School of Biological Sciences University of AucklandAucklandNew Zealand

For any given genotype,the environment in which an apple is grown can influence the properties of the fruit *** there has been extensive research on the mechanism of the genetic control of fruit quality traits,less effort has been made to investigate the way that these genetic mechanisms interact with the *** address this issue,we employed a large‘Royal Gala’×‘Braeburn’population of 572 seedlings replicated over sites in three climatically diverse apple-growing regions in New *** for traits including fruit maturation timing,firmness and dry matter content was performed at each of these three sites for a single growing season(2011),and at two sites(Motueka and Hawke’s Bay)for two seasons(2009 and 2010).The phenotype data collected over 2 years at two sites enabled the detection of 190 quantitative trait loci(QTL)that controlled these traits regardless of year or growing location,as well as some chromosomal loci that influenced the traits in a single given environment or *** those loci that were environmentally stable over three sites,there was an interdependency of fruit maturation date,dry matter content and storage potential within this population,with two regions on Linkage Groups(LGs)10 and 16 strongly *** these loci were used in a marker-assisted selection programme to select for progeny bearing firmer fruit,this would have the unintentional consequence of selecting,high dry matter content,later maturing *** addition,a further 113 new QTLs with a smaller effect were identified,some of which were exhibited only in a single growing environment,demonstrating the underlying complexity of control of traits determining fruit quality,in addition to the need for being aware of environmental effects when developing new apple varieties.

关键词： fir apple fruit

哺乳动物细胞对丝裂霉素诱导的DNA链间交联的修复

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中国肿瘤生物治疗杂志 2004年第4期11卷 285-290页

作者：郑胡镛王欣 amy j.Warren Randy j.Legerski 胡亚美李磊首都医科大学附属北京儿童医院北京100045 德州大学MD安德森癌症中心休斯顿77030

目的:探讨哺乳动物细胞能否修复DNA链间交联(imerstrand crosslink,IcL)。方法:构建带有丝裂霉素(mito mycin c,MMc)交联的质粒pcMV-Luc／IcL,将该交联质粒转染无修复酶缺陷和有修复酶缺陷的哺乳动物细胞系,观察细胞对 MMc链间交联的修... 详细信息

目的:探讨哺乳动物细胞能否修复DNA链间交联(imerstrand crosslink,IcL)。方法:构建带有丝裂霉素(mito mycin c,MMc)交联的质粒pcMV-Luc／IcL,将该交联质粒转染无修复酶缺陷和有修复酶缺陷的哺乳动物细胞系,观察细胞对 MMc链间交联的修复能力。结果:(1)无酶缺陷的哺乳动物细胞对DNA的修复能力很强,即使缺乏同源序列,也能有效地去除DNA链间交联;(2)修复过程需要许多酶类参与,其中与核苷酸切除修复(nucleotide excision repair,NER)相关的酶类起着关键性作用,提示NER参与MMc链间交联的修复过程;(3)序列分析表明,非同源性重组修复为易错修复(error-prone re pair)。结论:哺乳动物细胞能有效地修复MMc诱导的DNA链间交联,核苷酸切除修复是重要的修复途径。

关键词： DNA链间交联丝裂霉素c 核苷酸切除修复

A Novel LNP-Based chlamydiaSubunit Vaccine Formulation That Induces Th1 Responses without Upregulating IL-17 Provides Equivalent Protection in Mice as Formulations That Induced IL-17 and Th1 cytokines

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World journal of Vaccines 2020年第4期10卷 55-75页

作者： Melissa A. Boddicker Robin M. Kaufhold Kara S. cox Bob j. Lucas jinfu Xie Deborah D. Nahas Sinoeun Touch amy S. Espeseth Kalpit A. Vora julie M. Skinner Department of Infectious Diseases/Vaccines Merck & Co. Inc. West Point PA USA

We evaluated novel chlamydial vaccines, consisting of major outer membrane protein (MOMP) alone or in combination with polymorphic membrane proteins D (PmpD) and G (PmpG) using a c57BL/6 mouse model. Native MOMP (nMOMP) isolated from c. muridarum elementary bodies (EBs) and recombinant PmpD and PmpG proteins were adjuvanted with Monophosphoryl lipid A (MPLA), with either lipid nanoparticles (LNPs) or the cationic lipid dimethyldioctadecylammonium bromide (DDA). Antibody titers to c. muridarum nMOMP, and EBs were evaluated by ELISA, and T-cell responses were analyzed by intracellular cytokine staining (IcS). Protection from challenge was determined by qPcR. Vaccine immunized mice showed significantly higher antibody titers to nMOMP (P c. muridarum EBs (P compared to the adjuvant alone group. Antibody titers in vaccine groups with Monophosphoryl lipid A (MPLA) + LNP were higher as compared to the MPLA + DDA group (P cept for (cm nMOMP + PmpG + PmpD p73 + PmpD p82 + MPLA + DDA) vs (cm nMOMP + PmpG + PmpD p73 + PmpD p82 + MPLA + LNP) for both c. muridarum EBs and PmpG. IcS analysis showed more robust cD4 + T-cell responses (IFN-γ/IL-2/TNF-a) in the DDA and LNP groups compared to the adjuvant alone group. The DDA + MPLA gave robust Th17 responses in comparison to MPLA and LNP group. Mice immunized with chlamydia antigens also showed protection from c. muridarum challenge, by reduction in bacterial shedding for all groups (P compared to shedding from the adjuvant control. Both vaccine formulations generated robust immunological responses, and both were protective by reducing bacterial shedding after challenge. This data indicates equal protection can be achieved without the induction of Th17 responses.

关键词： LNP chlamydia IL-17 Mouse Model Th1 cytokines

Evaluating Potential Vaccine Antigens in both the chlamydia trachomatisand chlamydia muridarumIntravaginal Mouse challenge Models

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World journal of Vaccines 2019年第2期9卷 49-69页

作者： Robin M. Kaufhold Melissa A. Boddicker jodie A. Field Bob j. Lucas joseph M. Antonello amy S. Espeseth julie M. Skinner jon H. Heinrichs jeffrey G. Smith Department of Infectious Diseases/Vaccines MRL (West Point PA) Merck & Co. Inc. Kenilworth NJ USA Present Address Teva Pharmaceuticals West Chester Pennsylvania USA Department of Biometrics Research MRL (West Point PA) Merck & Co. Inc. Kenilworth NJ USA Present Address Sanofi Pasteur Swiftwater Pennsylvania USA Present Address Moderna Therapeutics Cambridge Massachusetts USA

Identifying relevant animal challenge models adds to the complexity of human vaccine development. Murine challenge models have been the most utilized animal model for chlamydia trachomatis vaccine development. The question arises as to whether the c. trachomatis or c. muridarum pre-clinical model is optimal. We compared c. muridarum and c. trachomatis intravaginal challenge models in a combined total of seventy-five studies evaluating potential vaccine candidates. In 100% (42/42) of c. muridarum studies, mice immunized with chlamydia elementary bodies (EB) demonstrated a significant reduction in urogenital bacterial shedding as measured by qPcR (p c. trachomatis studies. We have evaluated proposed vaccine antigens in both models and observed immunization with chlamydia major outer membrane protein (MOMP) vaccine formulations to be protective (p c. trachomatis model, and immunization with PmpD p82 translocator domain was not protective in either model. We also observed in both models that depletion of cD4+ T-cells in MOMP-immunized mice resulted in diminished protective immunity but animals were still able to reduce the infection level. In contrast, mice immunized with live EBs by intraperitoneal route did not require cD4+ T-cells to resolve urogenital infection from intravaginal challenge in either model. Overall, we have found the c. muridarum model to be a more robust, reliable, and reproducible model for vaccine antigen discovery.

关键词： chlamydia Mouse challenge Model Intravaginal challenge Sexually Transmitted Diseases

Association of latitude and altitude with adverse outcomes in patients with cOVID-19: The VIRUS registry

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World journal of critical care Medicine 2022年第2期11卷 102-111页

作者： Aysun Tekin Shahraz Qamar Romil Singh Vikas Bansal Mayank Sharma Allison M LeMahieu Andrew c Hanson Phillip j Schulte Marija Bogojevic Neha Deo Simon Zec Diana j Valencia Morales Katherine A Belden Smith F Heavner Margit Kaufman Sreekanth cheruku Valerie c Danesh Valerie M Banner-Goodspeed catherine A St Hill amy B christie Syed A Khan Lynn Retford Karen Boman Vishakha KKumar john c O'Horo juan Pablo Domecq Allan j Walkey Ognjen Gaj.c.Rahul Kashyap Salim Surani TheSociety of critical care Medicine(SccM)Discovery Viral Infection and Respiratory Illness Universal Study(VIRUS):cOVID-Registry Investigator Group Department of Anesthesiology Mayo ClinicRochesterMN 55905United States Post-baccalaureate Research Education Program Mayo Clinic College of Medicine and ScienceRochesterMN 55905United States Division of Pulmonary and critical care Medicine Department of Internal MedicineMayo ClinicRochesterMN 55905United States Division of clinical Trials and Biostatistics Department of Quantitative Health SciencesMayo ClinicRochesterMN 55905United States Alix School of Medicine Mayo ClinicRochesterMN 55905United States Division of Infectious Diseases Thomas Jefferson University HospitalPhiladelphiaPA 19107United States Prisma Health GreenvilleSC 29605United States Englewood Health EnglewoodNJ 07631United States Divisions of cardiothoracic Anesthesiology and critical care Medicine Department of Anesthesiology and Pain ManagementUT Southwestern Medical CenterDallasTX 75390United States center for Applied Health Research Baylor Scott and White HealthDallasTX 75246United States Department of Anesthesiology Critical Care and Pain MedicineBeth Israel Deaconess Medical CenterBostonMA 02215United States Allina Health MinneapolisMN 55407United States Department of critical care Atrium Health NavicentMaconGA 31201United States Division of critical care Medicine Mayo Clinic Health SystemMankatoMN 56001United States Society of critical care Medicine Mount ProspectIL 60056United States Division of Infectious Diseases Mayo ClinicRochesterMN 55905United States Division of Nephrology and Hypertension Department of Internal MedicineMayo ClinicRochesterMN 55905United States Pulmonary center Division of PulmonaryAllergyCritical Care and Sleep MedicineDepartment of MedicineEvans Center of Implementation and Improvement SciencesBoston University School of MedicineBostonMA 02118United States Department of Pulmonary and critical care Medicine Texas A&M UniversityBryanTX 77807United States 不详

BAcKGROUND The coronavirus disease 2019(cOVID-19)course may be affected by environmental *** studies previously suggested a link between climatological factors and cOVID-19 fatality ***,individual-level impact of these factors has not been thoroughly evaluated *** To study the association of climatological factors related to patient location with unfavorable outcomes in *** In this observational analysis of the Society of critical care Medicine Discovery Viral Infection and Respiratory Illness Universal Study:cOVID-19 Registry cohort,the latitudes and altitudes of hospitals were examined as a covariate for mortality within 28 d of admission and the length of hospital *** for baseline parameters and admission date,multivariable regression modeling was *** estimating equations were used to fit the *** Twenty-two thousand one hundred eight patients from over 20 countries were *** median age was 62(interquartile range:49-74)years,and 54%of the included patients were *** median age increased with increasing latitude as well as the frequency of ***,the percentage of comorbidities was lower in elevated *** within 28 d of hospital admission was found to be 25%.The median hospital-free days among all included patients was 20 *** the significant linear relationship between mortality and hospital-free days(adjusted odds ratio(aOR)=1.39(1.04,1.86),P=0.025 for mortality within 28 d of admission;aOR=-1.47(-2.60,-0.33),P=0.011 for hospital-free days),suggesting that adverse patient outcomes were more common in locations further away from the Equator;the results were no longer significant when adjusted for baseline differences(aOR=1.32(1.00,1.74),P=0.051 for 28-day mortality;aOR=-1.07(-2.13,-0.01),P=0.050 for hospital-free days).When we looked at the altitude’s effect,we discovered that it demonstrated a non-linear association with mortality wi

关键词： 28 d mortality Altitude cOVID-19 Hospital-free days Latitude Outcomes

2014年俄勒冈州一起与娱乐用天然湖泊有关联的诺如病毒暴发

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上海预防医学 2016年第6期28卷 429-431,F0004页

作者： amy Zlot Maayan Simckes jennifer Vines Laura Reynolds amy Sullivan Magdalena Kendall Scott j.Michael McLuckie Dan Kromer Vincent R.Hill jonathan S.Yoder Michele c.Hlavsa 宫宵欢陆仁敏 Multnomah county Health Department cDc/cSTE Applied Epidemiology Fellowship Program Oregon Public Health Division Metro Blue Lake Regional Park Fairview Oregon Division of Foodborne Waterborne and Environmental Diseases National Center for Emerging and Zoonotic Infectious Diseases CDC

2014年7月,摩特诺玛郡（Multnomah county）公共卫生人员对一起在俄勒冈州蓝湖地区公园的游客中发生的诺如病毒暴发进行了调查。在报告病例的周末（7月11日周五至7月13日周日）,约1.54万人入园游览。调查确定了65例诺如病毒感染疑似病例... 详细信息

2014年7月,摩特诺玛郡（Multnomah county）公共卫生人员对一起在俄勒冈州蓝湖地区公园的游客中发生的诺如病毒暴发进行了调查。在报告病例的周末（7月11日周五至7月13日周日）,约1.54万人入园游览。调查确定了65例诺如病毒感染疑似病例和5例诺如病毒感染实验室诊断病例（共70例）,未报告住院病例或死亡病例。回顾性队列研究发现。

关键词：诺如病毒俄勒冈州疑似病例天然湖泊卫生人员实验室诊断回顾性队列研究游泳者大肠埃希菌志贺菌

Erythrocyte c3d and c4d for monitoring disease activity in systemic lupus erythematosus

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Erythrocyte C3d and C4d for monitoring disease activity in s...

Optical Detection of Distal Lung Enzyme Activity in Human Inflammatory Lung Disease

The 8th International congress on SLE

作者： amy H.Kao jeannine S.Navratil Margie j.Ruffing chau-ching Liu Douglas Hawkins Andras Varadi Kathleen M. McKinnon joseph M.Abearn Susan Manzi University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine School of Statistics University of MinnesotaMinneapolisMinnesotaUSA University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine University of Pittsburgh School of Medicine University of Pittsburgh Graduate School of Public HealthPittsburghPennsylvaniaUSA

＜正＞Purpose:Disease activity monitoring in patients with systemic lupus erythematosus（SLE）is challenging,and traditionally has utilized serum c3 and c4 as an indirect measure of complement ***,these proteins have ...

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Biomedical Engineering Frontiers 2021年第1期2卷 44-54页

作者： Alicia Megia-Fernandez Adam Marshall Ahsan R.Akram Bethany Mills Sunay V.chankeshwara Emma Scholefield amy Miele Bruce c.McGorum chesney Michaels Nathan Knighton Tom Vercauteren Francois Lacombe Veronique Dentan Annya M.Bruce joanne Mair Robert Hitchcock Nik Hirani chris Haslett Mark Bradley Kevin Dhaliwal EaStcHEM The University of Edinburgh School of ChemistryJoseph Black BuildingWest Mains RoadEdinburghUK EH93FJ Translational Healthcare Technologies Group Centre for Inflammation ResearchQueen’s Medical Research InstituteUniversity of Edinburgh47 Little France CrescentEdinburgh BioQuarterEdinburghUK EH164TJ The Roslin Institute and Royal(Dick)School of Veterinary Studies University of EdinburghEaster BushMidlothianUK EH259RG Department of Biomedical Engineering University of Utah36 S Wasatch DrSalt Lake CityUT 84112USA School of Biomedical Engineering&Imaging Sciences King’s College LondonLondonUK SE17EH Mauna Kea Technologies 9Rue d’EnghienParisFrance 75010

Obj.c.ive and Impact *** is a need to develop platforms delineating inflammatory biology of the distal human *** describe a platform technology approach to detect in situ enzyme activity and observe drug inhibition in the distal human lung using a combination of matrix metalloproteinase(MMP)optical reporters,fibered confocal fluorescence microscopy(FcFM),and a bespoke delivery *** development of new therapeutic agents is hindered by the lack of in vivo in situ experimental methodologies that can rapidly evaluate the biological activity or drug-target engagement in *** optimised a novel highly quenched optical molecular reporter of enzyme activity(FIB One)and developed a translational pathway for in-human *** demonstrate the specificity for matrix metalloproteases(MMPs)2,9,and 13 and probe dequenching within physiological levels of MMPs and feasibility of imaging within whole lung models in preclinical ***,in a first-in-human exploratory experimental medicine study of patients with fibroproliferative lung disease,we demonstrate,through FcFM,the MMP activity in the alveolar space measured through FIB One fluorescence increase(with pharmacological inhibition).*** translational in situ approach enables a new methodology to demonstrate active drug target effects of the distal lung and consequently may inform therapeutic drug development pathways.

关键词： Lung lung specificity