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Characterization of amyloid-β precursor protein intracellular domain-associated transcriptional complexes in SH-SY5Y neurocytes

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Neuroscience Bulletin 2012年第3期28卷 259-270页

作者： Wulin Yang amy Yong chen Lau Shuizhong Luo Qian Zhu Li Lu School of Biotechnology and Food Engineering Hefei University of TechnologyHefei 230009China Laboratory of Metabolic Medicine Singapore Bioimaging Consortium(SBIC)Agency for ScienceTechnology and Research(A*STAR)Singapore Department of Anatomy Shanxi Medical UniversityTaiyuan 030001China

Objective Alzheimer＇s disease （AD） is one of the major disorders worldwide. Recent research suggests that the amyloid-β precursor protein intracellular domain （AICD） is a potential contributor to AD development and progression. The small AICD is rapidly degraded after processing from the full-length protein. The present study aimed to apply a highly efficient biotinylation approach in vitro to study AICD-associated complexes in neurocytes. Methods By coexpressing Escherichia coli biotin ligase with biotinyl-tagged AICD in the SH-SY5Y neuronal cell line, the effects of AICD overexpression on cell proliferation and apoptosis were analyzed. Besides, AICD-associated nuclear transcriptional complexes were purified and then examined by mass spectrometry. Results Our data showed that AICD overexpression not only affected cell proliferation but also led to apoptosis in differentiated SH-SY5Y cells. Moreover, biotinylation allowed single-step purification of biotinylated AICD-associated complexes from total nuclear extract via high-affinity biotin-streptavidin binding. Following this by mass spectrometry, we identified physically associated proteins, some reported previously and other novel binding partners, CUX1 and SPT5. Conclusion Based on these results, a map of the AICD-associated nuclear interactome was depicted. Specifically, AICD can activate CUX1 transcriptional activity, which may be associated with AICD-dependent neuronal cell death. This work helps to understand the AICD-associated biological events in AD progression and provides novel insights into the development of AD.

关键词： Alzheimer's disease amyloid-β precursor protein AICD biotinylation apoptosis

Multicultural Citizenship Education： A Study on Experiences and Opinions of Taiwan＇s Indigenous Elementary School Teachers

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Sociology Study 2013年第11期3卷 875-888页

作者： amy Shumin chen Tatung University Taiwan

The author reports findings of multicultural citizenship education from a two-year in-depth study of indigenous principals and teachers in local elementary schools. Data were gathered and analyzed using a conceptual framework that consisted of a multicultural citizenship based on the liberal theoretical perspective. The results indicate that indigenous educators face dilemmas in being cultural elites while considered national education policy implementer. Findings illustrate that indigenous elementary school educators are expected to possess differentiated-group privileges in teaching practices; however, they are confronted with both internal restrictions and external protections during establishing of ethnic cultural identity while playing their roles in the national educational apparatus. The paper also gives conclusions and suggestions for future researches.

关键词： Multicultural citizenship identity indigenous right education

Opportunities and Challenges for Global Student Mobility： A Study on Undergraduates＇ Experiences

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Sociology Study 2014年第5期4卷 385-394页

作者： amy Shumin chen Tatung University Taiwan

This paper examines the study abroad experiences of undergraduates, explores the students＇ values and perspectives on being global citizens, and expresses the way they think of themselves as members of the broader world community. The research was conducted by interviewing students who had experiences studying abroad. It aims to open a debate about the meanings and understandings of global student mobility which is based on the opinions and values of college students who had either long or short-term study abroad experiences. The study shows that students have grasped the transformation of globalization and are concerned with their competitiveness in the global stage. They pointed out that their higher education courses are lacking in providing enough opportunities for students to encounter the broader world. Also, students often cope with culture shock issues such as the way they interact with classmates, the attitude of contributing toward society, and the barrier to communicate using local languages. Proposed changes can start with professors, for example, by bringing the reality of other cultures into the students＇ learning process, by showing the common values and competencies of modern college students, and by contributing to critical thinking.

关键词： Globalization global student mobility study abroad pedagogy

The Experience of Model Minority for High- and Low-Income Asian American Students

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Sociology Study 2016年第4期6卷 211-218页

作者： amy Yun-Ping chen Saint Louis University USA

The author examines the differentiation of self-identity, school treatment, and academic struggle between two Asian American students in U.S. Midwest urban school environments. Using an interview study, the author focuses on understanding the students＇ perspectives in relation to the label of model minority. The purpose of the study is to investigate how social, academic, and economic factors affect these students, including different outcomes in terms of school achievement and self-identity formation. The findings aim to help urban educators approach complex factors regarding minority students＇ educational opportunities. Comprehensive results identified that：（1） The concept of model minority significantly affects Asian American students at all levels of daily life; （2） Urban schools continue reinforcing social reproduction and producing perceptions based on socioeconomic background and intersectional discrimination; （3） The evidence in the study shows that school environments do not aid students in valuing their cultural capital; （4） Students from different social classes present different linguistic and behavioral patterns; and （5） Social stratification significantly influences students＇ perspectives in response to the Asian stereotype, self-identity, and racial hierarchy in school and society.

关键词： Cultural capital racial hierarchy model minority self-identity social reproduction

Educational Inequality： An Impediment to True Democracy in the United States

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Sociology Study 2015年第5期5卷 382-390页

作者： amy Yun-Ping chen Saint Louis University USA

Many people believe that democracy is something that can lead everyone toward a better nation and a free and fair society. In 1954, Brown v. Board of Education fundamentally changed the education system in the United States and was a turning point in the evolution of U,S. democracy that made the beautiful American dream available for each individual. However, six decades later, the debate over educational inequality continues. People are still fighting for their equality and well-being. Along with dramatically influential political, economic, and social factors in the United States, educational inequality destroys the commitment of this democratic nation to offer equal opportunity, care, and social justice to younger members of the community. This paper discusses the current problem of educational inequality as it relates to racial and socioeconomic discrimination in urban schools. The aim of this study is to explore knowledge of inequality in the U.S. school system and to raise social and educational awareness to help American children to have equal chances in education and to achieve future success.

关键词： Democracy public education social mobility racial inequality socioeconomic inequality

Diagnosis,management and prevention of thrombotic complications in microsurgical breast reconstruction:a review of the literature

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Plastic and Aesthetic Research 2023年第1期10卷 389-414页

作者： amy chen Shannon R.Garvey Asha Nanda Bernard T.Lee Ryan P.Cauley Division of Plastic and Reconstructive Surgery Department of SurgeryBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMA 02215USA

Autologous free tissue transfer is a safe and effective option for breast *** is an increasingly utilized technique with well-demonstrated improved patient satisfaction and quality of *** thrombosis is a rare but significant complication of microsurgical breast reconstruction,often resulting in flap *** diagnosis and timely management of this complication are essential to free flap *** microvascular thrombosis poses a threat to flap survival,several methods may be employed to mitigate its more devastating ***,we present a comprehensive review of arterial and venous thrombotic complications in both the intraoperative and postoperative *** discuss preoperative risk assessment,methods for flap monitoring,and operative and medical management of thrombotic *** present an updated algorithm for the intraoperative management of microvascular thrombosis adapted to reflect the most recent literature and our novel algorithm for the postoperative management of microvascular thrombosis.

关键词： Breast microsurgery free flap monitoring free flap thrombosis free flap salvage

IMpower210:A phase Ⅲ study of second-line atezolizumab vs. docetaxel in East Asian patients with non-small cell lung cancer

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Chinese Journal of Cancer Research 2024年第2期36卷 103-113页

作者： Yi-Long Wu Shun Lu Gongyan chen Jianxing He Jifeng Feng Yiping Zhang Liyan Jiang Hongming Pan Jianhua Chang Jian Fang amy Cai Lilian Bu Jane Shi Jinjing Xia Guangdong Lung Cancer Institute Guangdong Provincial Key Laboratory of Translational Medicine in Lung CancerGuangdong Provincial People's Hospital&Guangdong Academy of Medical SciencesSouthern Medical UniversityGuangzhou 510080China Shanghai Lung Cancer Center Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Respiration Harbin Cancer HospitalHarbin Medical UniversityHarbin 150081China Department of Thoracic Oncology and Surgery the First Affliated Hospital of Guangzhou Medical UniversityGuangzhou 510120China Department of Medical Oncology Jiangsu Cancer HospitalNanjing 210009China Department of Medical Oncology Zhejiang Cancer HospitalHangzhou 330022China Department of Pulmonary Medicine Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200030China Department of Medical Oncology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Medical Oncology Shenzhen HospitalCancer Hospital of Chinese Academy of Medical SciencesShenzhen 518116China Department of Thoracic Oncology Beijing Cancer HospitalBeijing 100142China Product Development Shanghai Roche Pharmaceutical LtdShanghai 201203China

Objective: IMpower210(NCT02813785) explored the efficacy and safety of single-agent atezolizumab *** as second-line treatment for advanced non-small cell lung cancer(NSCLC) in East Asian ***: Key eligibility criteria for this phase Ⅲ, open-label, randomized study included age ≥18 years;histologically documented advanced NSCLC per the Union for International Cancer Control/American Joint Committee on Cancer staging system(7th edition);Eastern Cooperative Oncology Group performance status of 0 or 1;and disease progression following platinum-based chemotherapy for advanced or metastatic NSCLC. Patients were randomized 2:1 to receive either atezolizumab(1,200 mg) or docetaxel(75 mg/m^(2)). The primary study endpoint was overall survival(OS) in the intention-to-treat(ITT) population with wild-type epidermal growth factor receptor expression(ITT EGFR-WT) and in the overall ITT ***: Median OS in the ITT EGFR-WT population(n=467) was 12.3 [95% confidence interval(95% CI),10.3-13.8] months in the atezolizumab arm(n=312) and 9.9(95% CI, 7.8-13.9) months in the docetaxel arm[n=155;stratified hazard ratio(HR), 0.82;95% CI, 0.66-1.03]. Median OS in the overall ITT population was 12.5(95% CI, 10.8-13.8) months with atezolizumab treatment and 11.1(95% CI, 8.4-14.2) months(n=377) with docetaxel treatment(n=188;stratified HR, 0.87;95% CI, 0.71-1.08). Grade 3/4 treatment-related adverse events(TRAEs) occurred in 18.4% of patients in the atezolizumab arm and 50.0% of patients in the docetaxel ***: IMpower210 did not meet its primary efficacy endpoint of OS in the ITT EGFR-WT or overall ITT populations. Atezolizumab was comparatively more tolerable than docetaxel, with a lower incidence of grade3/4 TRAEs.

关键词： Atezolizumab East Asia non-small cell lung cancer programmed death-ligand 1 inhibitors monoclonal antibody

Hydraulic properties and drought response of a tropical bamboo (Cephalostachyum pergracile)

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Plant Diversity 2024年第3期46卷 406-415页

作者： Wanwalee Kongjarat Lu Han amy Ny Aina Aritsara Shu-Bin Zhang Gao-Juan Zhao Yong-Jiang Zhang Phisamai Maenpuen Ying-Mei Li Yi-Ke Zou Ming-Yi Li Xue-Nan Li Lian-Bin Tao Ya-Jun chen CAS Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesYunnan 666303China University of the Chinese Academy of Sciences Beijing 100049China T-STAR Core Team Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesYunnan 666303China School of Biology and Ecology University of MaineOronoMEUSA Climate Change Institute University of MaineOronoME 04469USA School of Biological and Chemical Science Pu’er UniversityXueyuan RoadYunnan 665000China Institute of Ecology and Geobotany School of Ecology and Environmental SciencesYunnan UniversityKunmingYunnan 650091China School of Ecology and Environment Southwest Forestry UniversityKunmingYunnan 650224China Yuanjiang Savanna Ecosystem Research Station Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesYuanjiangYunnan 653300China

Bamboo plants are an essential component of tropical ecosystems,yet their vulnerability to climate extremes,such as drought,is poorly understood due to limited knowledge of their hydraulic *** pergracile,a commonly used tropical bamboo species,exhibited a substantially higher mortality rate than other co-occurring bamboos during a severe drought event in 2019,but the underlying mechanisms remain *** study investigated the leaf and stem hydraulic traits related to drought responses,including leaf-stem embolism resistance(P50leaf;P50stem) estimated using optical and X-ray microtomography methods,leaf pressure-volume and water-releasing ***,we investigated the seasonal water potentials,native embolism level(PLC) and xylem water source using stable *** found that *** exhibited strong resistance to embolism,showing low P50leaf,P50stem,and turgor loss point,despite its rapid leaf water ***,its leaves displayed greater resistance to embolism than its stem,suggesting a lack of effective hydraulic vulnerability segmentation(HVS) to protect the stem from excessive xylem *** the dry season,approximately 49% of the water was absorbed from the upper 20-cm-deep soil ***,significant diurnal variation in leaf water potentials and an increase in midday PLC from 5.87±2.33% in the wet season to 12.87±4.09%in the dry season were *** summary,this study demonstrated that the rapid leaf water loss,high reliance on surface water,and a lack of effective HVS in *** accelerated water depletion and increased xylem embolism even in the typical dry season,which may explain its high mortality rate during extreme drought events in 2019.

关键词： Climate change Drought Hydraulic safety Hydraulic vulnerability segmentation Stable isotope Tree mortality

Generation and characterization a conditional mouse model of Parkinson’s disease

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Generation and characterization a conditional mouse model of...