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PHYSIOLOGICAL INHIBITORY EFFECT OF OCS IN ARACHIDONIC ACID-RICH PARIETOCHLORIS INCISA (TREBOUXIOPHYCEAE, CHLOROPHYTA)

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Chinese Journal of Oceanology and Limnology 2002年第3期20卷 248-255页

作者：刘建国张成武 Zvi Cohen amos richmond Research and Development Center of Marine Biotechnology Institute of Oceanol ogy Chinese Academy of Sciences Qingdao 266071 China Division of life Sciences Nanjing Normal University Nanjing 210097 China Microalgal Biotechnology The Jacob Blaustein Institute for Desert Research Ben-Gurion University of Negev Sede Boker Campus 84993 Israel

Parietochloris incisa is an arachidonic acid rich snow green alga. The main physiological profiles, such as ash free dry weight (AFDW), chlorophyll, carotenoid, protein and total fatty acids (TFA), in this alga exposed to old culture supernatant (OCS) at the decline phase or its crude ethyl acetate extracts (CEAE) were investigated by using tubular photobioreactors of different diameters. Results showed that both OCS and CEAE had strong inhibitory effect on the above physiological parameters. The longer the culture was exposed to OCS and the more CEAE were added into the algal culture, the more the above physiological properties were inhibited. Arachidonic acid (AA), the dominant component of fatty acids in this alga, was also seriously inhibited with respect to total TFA, AFDW of cell mass, or culture volume, due to a probable reduction of enzymes activities catalyzing chain elongation from C18:1ω9 to AA. These results incontestably evidenced that some CEAE dissolving substances existing in OCS, like auto inhibitors, inhibited P. incisa growth through feedback. Hence, any efficient removal of auto inhibitors from algal culture to decrease their bioactivity could be good for maximal production of desired products like AA.

关键词： arachidonic acid Parietochloris incisa growth auto inhibitor

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雪藻高密度连续培养中生物量和花生四烯酸的高产率

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海洋与湖沼 2002年第5期33卷 499-508页

作者：刘建国刘伟 Zvi Cohen amos richmond 中国科学院海洋研究所海洋生物技术研究发展中心青岛266071 Microalgal Biotechnology The Jacob Blaustein Institute for Desert Research Ben-Gurion University of the Negev

以 1 .4cm和 2 .8cm光径的平板光生物反应器 ,恒定高光强 [2 4 0 0 μE/(m2 ·s) ],通气、无菌高密度连续培养雪地绿藻 (简称雪藻 )。连续采收并补充等量新鲜培养液 ,当藻体生物量(以去灰分干重表示 ,AFDW)达恒态时 ,研究雪藻比生长速率... 详细信息

以 1 .4cm和 2 .8cm光径的平板光生物反应器 ,恒定高光强 [2 4 0 0 μE/(m2 ·s) ],通气、无菌高密度连续培养雪地绿藻 (简称雪藻 )。连续采收并补充等量新鲜培养液 ,当藻体生物量(以去灰分干重表示 ,AFDW)达恒态时 ,研究雪藻比生长速率 (SGR)、藻体的生物产量、总脂肪酸 (TFA)和花生四烯酸 (AA)产率。结果表明 ,尽管随着比生长速率增加 ,雪藻高密度连续培养体系的AA/TFA、TFA/AFDW和AA/AFDW三个指标有不同程度的降低 ,但生物产量、总脂肪酸及其花生四烯酸产出都相当高。 2 .8cm光径的平板光生物反应器中 ,雪藻SGR为0 .2d- 1 时 ,每平方米光照面积日获 35 .3gAFDW的高产量 ;SGR 0 .1 35d- 1 时 ,TFA产率最大 ,为7.0g/(m2 ·d) ,此时AA产率也最高 ,达 2 .6g/(m2 ·d)。尽管 1 .4cm光径的反应器的培养体积比前者下降了 5 0 % ,但细胞浓度明显提高 ,单位光照面积的AFDW和TFA最大日产率只下降了37.3%和 1 0 % ,分别为 2 2 .1和 6.3g/(m2 ·d) ,而AA的最大日产率也是 2 .6g/(m2 ·d)。结果还表明 ,氮或磷营养亏缺能明显提高雪藻AA含量的各项指标 ,同时高光强也有利于雪藻累积AA。

关键词：雪藻高密度培养平板光生物反应器花生四烯酸产率比生长速率绿藻

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平板式光生物反应器的Parietochloris incisa超高密度培养

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烟台大学学报（自然科学与工程版） 2000年第3期13卷 230-234页

作者：王长海 amos richmond 烟台大学海洋生化工程研究所山东烟台264635 本.古里安大学勃鲁斯坦沙漠研究学院微藻生物技术实验室以色列sedeboker84990

利用平板式光生物反应器对一种新分离的微藻Parietochlorisincisa进行了室外放大培养研究。在最适培养条件下 ,实现了对该微藻的超高密度培养 ,使单位培养体积和单位培养面积的细胞生物量分别达到了 7.35g/ (L·d)和 96 .12 g/ (m2 ·d)

关键词：平板式光生物反应器超高密度培养微藻

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雪藻高密度连续培养中生物量和花生四烯酸的高产出

雪藻高密度连续培养中生物量和花生四烯酸的高产出

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中国藻类学会第十一次学术讨论会

作者：刘建国 Zvi Cohen amos richmond 中国科学院海洋研究所海洋生物技术研究发展中心 Microalgal Biotechology The Jacob Blaustein Institute for Desert Research Ben-Gurion University of the NegevSede Boker Campus 84993Israel

以1.4和2.8cm光径的平板光生物反应器,和相当于自然光照强度的恒定高光强(2400μE·M·S),通气、无菌高密度连续培养雪地绿藻(简称雪藻),并以系列速率连续采收,同时补充等量新鲜培养液,当培养体系藻体生物量(以去灰分干重表示,AFDW)达... 详细信息

以1.4和2.8cm光径的平板光生物反应器,和相当于自然光照强度的恒定高光强(2400μE·M·S),通气、无菌高密度连续培养雪地绿藻(简称雪藻),并以系列速率连续采收,同时补充等量新鲜培养液,当培养体系藻体生物量(以去灰分干重表示,AFDW)达到恒态时,研究了雪藻比生长速率与藻体生物量、总脂肪酸(TFA)和花生四烯酸(AA)产率的关系。随着比生长速率的增加,雪藻的AA/TFA、TFA/AFDW和AA/AFD表现出程度不一但都相类似的变化规律,即三个指标均逐渐阵低.就2.8。m光径的平板光生物反应器产AFI)W而言,以比生长速串0.2时最大,每平方米光照面积日获35.3克的高产量;而以0.l的比生长速串生长时,AA/H比串最高;TFA产串以比生长速率0.135时最大,为 7.0 g·。-’·d-‘,此时的 AA产率也最高,达2.6 g·m”寸‘.对比二.4和***光径的平板光生物反应器的培养结果发现:尽管窄光径条件下,总的培养体积诚少了一半,但是单位体积内的生物量浓度捉高了,单位光照面积的AFl)W和Wb的最大日产率分别只下降了37 3你和lthe,为22.l和6.3 g·m-’·d’;d的最大日产串井没有变化,都是2.6 g·。”寸‘。本研究同时表明:汉或磷营养亏缺能明显提高雪藻M含量的各项指标,高光强也有利于雪藻昆积AA.

关键词：雪藻高密度培养花生四烯酸比生长速率

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Targeted community based interventions improved malaria management competencies in rural Ghana

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Global Health Research and Policy 2017年第1期2卷 64-73页

作者： Isabella A.Quakyi George O.Adjei David J.Sullivan Jr Judith K.Stephens amos Laar Vivian N.Ama Aubyn richmond Owusu Kwame S.Sakyi Nathaniel Coleman Francis D.Krampa Linda Vanotoo Julliette Tuakli Bernard B.Bortei Edward Essuman Felix Sorvor Isaac A.Boateng Constance Bart-Plange Ebenezer A.Addison Peter Winch Andrew A.Adjei Department of Biological Environmental and Occupational Health SciencesSchool of Public HealthCollege of Health SciencesUniversity of GhanaP.O.Box LG 13LegonAccraGhana Office of Research Innovation and DevelopmentUniversity of GhanaLegonAccraGhana Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health615 N.Wolfe StBaltimoreMD 21205USA Department of Population Familyand Reproductive HealthSchool of Public HealthUniversity of GhanaLegonAccraGhana National Malaria Control Programme Ministry of HealthAccraGhana Department of Health Policy Planning and ManagementSchool of Public HealthUniversity of GhanaLegonAccraGhana Department of International Health Social and Behavioural Interventions ProgramJohns Hopkins Bloomberg School of Public Health615 N.Wolfe StBaltimoreMD 21205USA Department of Biochemistry Cell and Molecular BiologyUniversity of GhanaLegonAccraGhana Regional Health Directorate Ghana Health ServicesAccraGhana Child and Associates AccraGhana Asante-Akim Central Municipal Health Directorate Ghana Health ServicesKonongoGhana Kpone Katamanso District Health Directorate Ghana Health ServicesKponeTemaGhana Worldwide Universities Network University of GhanaP.O.Box LG 13LegonAccraGhana

Background:Malaria is one of the most challenging public health concerns in the developing *** address its impact in endemic regions,several interventions are implemented by *** Affordable Medicine Facility-malaria(AMFm)is an example of such *** activities include communication interventions to enhance the knowledge of caregivers of children under five years,licensed chemical sellers(LCS)and prescribers on malaria management with artemisinin-based combination therapy(ACT).This study was conducted to evaluate the effectiveness of the AMFm activities on malaria among targeted groups in two rural communities in ***:A communication intervention study was conducted in the Asante-Akim North and South Districts of *** cross-sectional pre and post surveys were *** malaria messages were designed and used to develop the information,education and communication(IEC)tools for the *** the aid of posters and flipcharts developed by our study,community health workers(CHWs),prescribers,and licenced chemical sellers provided proper counselling to clients on malaria *** CHWs and community based volunteers educated caregivers of children under five years on malaria management at their homes and at public gatherings such as churches,mosques,***-square tests and logistic regression were run to determine associations and control for demographic differences ***:There was significantly high exposure to malaria/ACT interventions in the intervention district than in the comparison district(OR=16.02;95%CI=7.88–32.55)and same for malaria/ACT-related knowledge(OR=3.63;95%CI=2.52–5.23).The participants in the intervention district were also more knowledgeable about correct administration of dispersible drug for children<5 years than their counterparts in the unexposed ***:Our data show that targeted interventions improve malaria based competences in rural commun

关键词： malaria district gathering

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